KEGG   ENZYME: 2.1.1.209Help
Entry
EC 2.1.1.209                Enzyme                                 

Name
23S rRNA (guanine2535-N1)-methyltransferase;
AviRa
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:23S rRNA (guanine2535-N1)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + guanine2535 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
guanine2535 in 23S rRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N1-methylguanine2535 in 23S rRNA
Comment
Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis. To protect itself from the antibiotic, Streptomyces viridochromogenes utilizes two methyltransferases, 23S rRNA (guanine2535-N1)-methyltransferase and EC 2.1.1.208 [23S rRNA (uridine2479-2-O)-methyltransferase], whose actions confer avilamycin resistance to the RNA.
History
EC 2.1.1.209 created 2011
Orthology
K21515  23S rRNA (guanine2535-N1)-methyltransferase
Genes
BLE: BleG1_3359
GYM: GYMC10_5407
TCO: Theco_1547
THL: TEH_05890
CPY: Cphy_2160
SKE: Sked_37520
NDA: Ndas_4431
NAL: B005_2412(aviRa)
SRO: Sros_4106
PSEA: WY02_14740
PSEE: FRP1_27745
PSEH: XF36_26020
ACTI: UA75_10110
ACAD: UA74_10135
 » show all
Taxonomy
Reference
1  [PMID:12828631]
  Authors
Treede I, Jakobsen L, Kirpekar F, Vester B, Weitnauer G, Bechthold A, Douthwaite S
  Title
The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the  guanosine 2535 base and the uridine 2479 ribose.
  Journal
Mol Microbiol 49:309-18 (2003)
DOI:10.1046/j.1365-2958.2003.03558.x
  Sequence
Reference
2  [PMID:11181344]
  Authors
Weitnauer G, Gaisser S, Trefzer A, Stockert S, Westrich L, Quiros LM, Mendez C, Salas JA, Bechthold A
  Title
An ATP-binding cassette transporter and two rRNA methyltransferases are involved  in resistance to avilamycin in the producer organism Streptomyces viridochromogenes Tu57.
  Journal
Antimicrob Agents Chemother 45:690-5 (2001)
DOI:10.1128/AAC.45.3.690-695.2001
  Sequence
Reference
3  [PMID:12742024]
  Authors
Mosbacher TG, Bechthold A, Schulz GE
  Title
Crystal structure of the avilamycin resistance-conferring methyltransferase AviRa from Streptomyces viridochromogenes.
  Journal
J Mol Biol 329:147-57 (2003)
DOI:10.1016/S0022-2836(03)00407-8
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.209
IUBMB Enzyme Nomenclature: 2.1.1.209
ExPASy - ENZYME nomenclature database: 2.1.1.209
BRENDA, the Enzyme Database: 2.1.1.209

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