KEGG   ENZYME: 2.1.1.215Help
Entry
EC 2.1.1.215                Enzyme                                 

Name
tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase;
Trm1 (ambiguous);
tRNA (N2,N2-guanine)-dimethyltransferase;
tRNA (m2(2G26) methyltransferase;
Trm1[tRNA (m2(2)G26) methyltransferase]
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase
Reaction(IUBMB)
4 S-adenosyl-L-methionine + guanine26/guanine27 in tRNA = 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26/N2-dimethylguanine27 in tRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
guanine26/guanine27 in tRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N2-dimethylguanine26/N2-dimethylguanine27 in tRNA
Comment
The enzyme from Aquifex aeolicus is similar to the TRM1 methyltransferases of archaea and eukarya (see EC 2.1.1.216, tRNA (guanine26-N2)-dimethyltransferase). However, it catalyses the double methylation of guanines at both positions 26 and 27 of tRNA.
History
EC 2.1.1.215 created 2011 (EC 2.1.1.32 created 1972, part transferred 2011 to EC 2.1.1.215)
Orthology
K00555  tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase
Genes
HSA: 55621(TRMT1)
PTR: 455758(TRMT1)
PPS: 100990135(TRMT1)
GGO: 101151313(TRMT1)
PON: 100454032(TRMT1)
NLE: 100602011(TRMT1)
MCC: 718227(TRMT1)
CSAB: 103234011(TRMT1)
RRO: 104661074(TRMT1)
RBB: 108544104(TRMT1)
CJC: 100389881(TRMT1)
SBQ: 101041404(TRMT1)
MMU: 212528(Trmt1)
RNO: 288914(Trmt1)
CGE: 100765046(Trmt1)
NGI: 103751868(Trmt1)
HGL: 101723979(Trmt1)
CCAN: 109681261(Trmt1)
OCU: 100350974(TRMT1)
TUP: 102473074(TRMT1)
CFA: 476692(TRMT1)
AML: 100473704(TRMT1)
UMR: 103682140(TRMT1)
ORO: 101376712(TRMT1)
FCA: 101094328(TRMT1)
PTG: 102963269(TRMT1)
AJU: 106986093(TRMT1)
BTA: 539613(TRMT1)
BOM: 102277611(TRMT1)
BIU: 109561518(TRMT1)
PHD: 102340657(TRMT1)
CHX: 102176952(TRMT1)
OAS: 101121680(TRMT1)
SSC: 100627711(TRMT1)
CFR: 102513537(TRMT1)
CDK: 105100763(TRMT1)
BACU: 103008602(TRMT1)
LVE: 103079920(TRMT1)
OOR: 101277455(TRMT1)
ECB: 100146178(TRMT1)
EPZ: 103567234(TRMT1)
EAI: 106844329(TRMT1)
MYB: 102248201(TRMT1)
MYD: 102754350(TRMT1)
HAI: 109394288(TRMT1)
RSS: 109454975(TRMT1)
PALE: 102883077(TRMT1)
LAV: 100655459(TRMT1)
TMU: 101352949
MDO: 100027314(TRMT1)
SHR: 100925495(TRMT1)
PHI: 102101496(TRMT1)
FPG: 101916870(TRMT1)
FCH: 106630862(TRMT1)
AAM: 106498059(TRMT1)
ASN: 102376457(TRMT1)
AMJ: 102561841(TRMT1)
PSS: 102446740(TRMT1)
CMY: 102934172(TRMT1)
CPIC: 101942796(TRMT1)
ACS: 100557970(trmt1)
PVT: 110082638(TRMT1)
PBI: 103065048(TRMT1)
GJA: 107114393(TRMT1)
XLA: 100158298(trmt1.L) 108703489
XTR: 779562(trmt1)
NPR: 108794968(TRMT1)
DRE: 553298(trmt1)
SGH: 107556090(trmt1) 107581597
IPU: 108259495(trmt1)
AMEX: 103032882(trmt1)
TRU: 101073165(trmt1)
LCO: 104924534(trmt1)
MZE: 101487551(trmt1) 112434923
OLA: 101168669(trmt1)
XMA: 102226647(trmt1)
PRET: 103474063(trmt1)
NFU: 107391290(trmt1)
KMR: 108230061(trmt1)
CSEM: 103383430(trmt1)
LCF: 108880839(trmt1)
SDU: 111235867(trmt1)
HCQ: 109509013 109511066(trmt1)
BPEC: 110173983
MALB: 109970349(trmt1)
SASA: 100380529(trmt1) 106590623
ELS: 105012091(trmt1)
SFM: 108927503(trmt1)
LCM: 102355111(TRMT1)
CMK: 103172159(trmt1)
CIN: 100185676
SPU: 585741(trmt1)
APLC: 110978622
SKO: 100377630
DME: Dmel_CG6388(CG6388)
DSI: Dsimw501_GD23837(Dsim_GD23837)
MDE: 101899661
AAG: 5573251
AME: 412163
BIM: 105680525
BTER: 105666548
AEC: 105146507
ACEP: 105625457
PBAR: 105430038
HST: 105186365
DQU: 106744717
CFO: 105250385
LHU: 105670793
PGC: 109862923
PCF: 106786970
NVI: 100120072
MDL: 103572384
TCA: 654887
DPA: 109544566
NVL: 108568775
BMOR: 101738076
PMAC: 106714674
PRAP: 111003273
HAW: 110378855
DNX: 107161567
CLEC: 106666067
ZNE: 110835743
FCD: 110853929
TUT: 107361647
CEL: CELE_ZC376.5(trm-1)
CBR: CBG04743(Cbr-trm-1)
BMY: Bm1_16350
TSP: Tsp_02445
CRG: 105345140
OBI: 106882066
SHX: MS3_01070
EGL: EGR_03191
EPA: 110243239
ADF: 107355120
HMG: 100201984
LJA: Lj0g3v0159199.1(Lj0g3v0159199.1) Lj0g3v0302299.1(Lj0g3v0302299.1) Lj0g3v0302739.1(Lj0g3v0302739.1) Lj1g3v1182040.1(Lj1g3v1182040.1) Lj1g3v1182040.2(Lj1g3v1182040.2)
DOSA: Os03t0786700-01(Os03g0786700) Os05t0324200-01(Os05g0324200) Os10t0357800-01(Os10g0357800)
ATS: 109747522(LOC109747522) 109762476(LOC109762476) 109785912(LOC109785912)
ZMA: 100274276 100275038 100276957(si605032f04) 100382564(si605022b11(700))
APRO: F751_4586
SCE: YDR120C(TRM1)
ERC: Ecym_4296
KMX: KLMA_40150(TRM1)
NCS: NCAS_0B03760(NCAS0B03760)
NDI: NDAI_0J01300(NDAI0J01300)
TPF: TPHA_0A01730(TPHA0A01730)
TBL: TBLA_0H01440(TBLA0H01440)
TDL: TDEL_0F04010(TDEL0F04010)
KAF: KAFR_0B05430(KAFR0B05430)
PIC: PICST_65944(TRM3)
CAL: CAALFM_CR00900WA(TRM1)
CAUR: QG37_01692
SLB: AWJ20_4100(TRM1)
NCR: NCU06336
NTE: NEUTE1DRAFT60425(NEUTE1DRAFT_60425)
MGR: MGG_01448
SSCK: SPSK_00432
MAW: MAC_08423
MAJ: MAA_06435
CMT: CCM_04696
BFU: BCIN_01g01230(Bctrm1)
MBE: MBM_05244
ANI: AN9406.2
ANG: ANI_1_2058014(An01g00070)
ABE: ARB_06049
TVE: TRV_06924
PTE: PTT_11253
SPO: SPBC25D12.05(trm1)
CNE: CNA00760
CNB: CNBA0740
ABP: AGABI1DRAFT97610(AGABI1DRAFT_97610)
ABV: AGABI2DRAFT183183(AGABI2DRAFT_183183)
MGL: MGL_0388
DFA: DFA_03998
EHI: EHI_148760(1.t00080)
PYO: PY17X_1419500(PY04503)
PCB: PCHAS_141960(PC000082.01.0)
TAN: TA13660
TPV: TP02_0525
BBO: BBOV_II005710(18.m06475)
CPV: cgd4_3250
SMIN: v1.2.034519.t1(symbB.v1.2.034519.t1) v1.2.037272.t1(symbB.v1.2.037272.t1)
TCR: 511857.40
SYW: SYNW0682
SYC: syc1676_d
SYG: sync_0902
SYR: SynRCC307_1690(trm1)
SYX: SynWH7803_1642(trm1)
SYNR: KR49_05155
SYND: KR52_06330
SYNW: SynWH8103_00781(trm1)
HHG: XM38_002090(trm1)
PMT: PMT_1174
AMR: AM1_3892
MPK: VL20_30
CYT: cce_1559
AAE: aq_841(trm1)
HTH: HTH_0773
TAL: Thal_0545
MJA: MJ_0946
MMP: MMP0228(trm1)
MMD: GYY_01160
MAE: Maeo_0624
MVO: Mvol_1468
MTH: MTH_1190
MMG: MTBMA_c15670(trm)
METC: MTCT_1083
MWO: MWSIV6_1583(trm1)
METE: tca_01146(trm1_1)
MST: Msp_0291(trm1)
MSI: Msm_1031
MRU: mru_1960(trm1)
MEB: Abm4_1543(trm1)
MMIL: sm9_2043(trm1)
MEYE: TL18_09400
MOL: YLM1_1407
METH: MBMB1_1709(trm1)
MFC: BRM9_0961
MFI: DSM1535_0931(trm1)
MCUB: MCBB_2007(trm1)
MFV: Mfer_1045
MKA: MK0154(trm1_1)
AFU: AF_0815
FPL: Ferp_1309
GAC: GACE_1318
GAH: GAH_00093
TAC: Ta0997
TVO: TVG0752240(TVG0752240)
PTO: PTO0220
FAI: FAD_1424
CDIV: CPM_1187
MEAR: Mpt1_c06940(trm1)
MARC: AR505_1166
ABI: Aboo_0297
PHO: PH1829(PH1829)
PAB: PAB2092
PFU: PF1871
PFI: PFC_09230
PYN: PNA2_0427
PYS: Py04_1759
TKO: TK0970
TON: TON_0648
TGA: TGAM_1392(trm1)
TSI: TSIB_0515
THE: GQS_01745
THA: TAM4_2149
THM: CL1_0098
TLT: OCC_07861
THS: TES1_0378
TNU: BD01_0741
TEU: TEU_07760
PPAC: PAP_03145
MAC: MA_1455(trm1)
MBAR: MSBR2_0542
MBAK: MSBR3_0426
MMA: MM_2528
MMAC: MSMAC_2278
METM: MSMTP_2033
MTHE: MSTHC_0097
MTHR: MSTHT_0623
MHOR: MSHOH_2781
MBU: Mbur_0659
MMET: MCMEM_0802
MMH: Mmah_1144
MPY: Mpsy_3095
MTP: Mthe_1426
MCJ: MCON_1370(trm1)
MHI: Mhar_0029
MHU: Mhun_0626
MLA: Mlab_0794
MBG: BN140_0747(trm1)
MPI: Mpet_0923
MBN: Mboo_1845
MPL: Mpal_2545
MPD: MCP_1029(trm1)
MEZ: Mtc_1921(trm1)
RCI: RCIX2707(trm1)
HAL: VNG_2088G(trm1)
HSL: OE_3917F(trm1)
HHB: Hhub_3381(trm1)
HALH: HTSR_1892(trm1)
HHSR: HSR6_1962(trm1)
HSU: HLASF_2008(trm1)
HSF: HLASA_1994(trm1)
HMA: rrnAC2788(trm1)
HHI: HAH_0070(trm1)
NPH: NP_0194A(trm1)
NMO: Nmlp_1115(trm1)
HUT: Huta_1529
HTI: HTIA_1514
HMU: Hmuk_0713
HWA: HQ_3646A(trm1)
HWC: Hqrw_4058(trm1)
HVO: HVO_0236(trm1)
HME: HFX_0240(trm1)
HLA: Hlac_2358
HTU: Htur_3148
NMG: Nmag_1456(trm1)
NAT: NJ7G_1665
SALI: L593_05775
APE: APE_0782.1(trm1)
ACJ: ACAM_0549(trm1)
SMR: Smar_1252
IHO: Igni_1184
IIS: EYM_03930
DKA: DKAM_0811
TAG: Tagg_0152
IAG: Igag_0019
HBU: Hbut_0460
STO: STK_12690(trm1)
SSO: SSO0943
SOL: Ssol_1919
SSOA: SULA_1951
SSOL: SULB_1952
SSOF: SULC_1950
SAI: Saci_1343(trm1)
SID: M164_1267
SII: LD85_1395
SIH: SiH_1228
SIR: SiRe_1146
SIC: SiL_1143
MSE: Msed_1726
MCN: Mcup_0502
AHO: Ahos_1391
PAI: PAE1628
PIS: Pisl_1656
PCL: Pcal_0595
PAS: Pars_1987
PYR: P186_0010
POG: Pogu_0135
TNE: Tneu_0683
CMA: Cmaq_1309
TUZ: TUZN_2061
TTN: TTX_1473(trm)
VDI: Vdis_0307
VMO: VMUT_1325
TPE: Tpen_0379
ASC: ASAC_0213
NMR: Nmar_1394
NCT: NMSP_0351(trm1)
NGA: Ngar_c34550(trm1)
NVN: NVIE_017420(trm)
NEV: NTE_00628
TAA: NMY3_02258(trm1)
NCV: NCAV_0735(trm)
NBV: T478_1086
NDV: NDEV_1682
NEQ: NEQ108
NAA: Nps_01040
MARH: Mia14_0212
KCR: Kcr_1510
BARC: AOA65_0500(trm1)
BARB: AOA66_1039(trm1)
LOKI: Lokiarch_25110(trm1)
 » show all
Taxonomy
Reference
1  [PMID:19491098]
  Authors
Awai T, Kimura S, Tomikawa C, Ochi A, Ihsanawati, Bessho Y, Yokoyama S, Ohno S, Nishikawa K, Yokogawa T, Suzuki T, Hori H
  Title
Aquifex aeolicus tRNA (N2,N2-guanine)-dimethyltransferase (Trm1) catalyzes transfer of methyl groups not only to guanine 26 but also to guanine 27 in tRNA.
  Journal
J Biol Chem 284:20467-78 (2009)
DOI:10.1074/jbc.M109.020024
  Sequence
[aae:aq_841]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.215
IUBMB Enzyme Nomenclature: 2.1.1.215
ExPASy - ENZYME nomenclature database: 2.1.1.215
BRENDA, the Enzyme Database: 2.1.1.215

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