KEGG   ENZYME: 2.1.1.230Help
Entry
EC 2.1.1.230                Enzyme                                 

Name
23S rRNA (adenosine1067-2'-O)-methyltransferase;
23S rRNA A1067 2'-methyltransferase;
thiostrepton-resistance methylase;
nosiheptide-resistance methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:23S rRNA (adenosine1067-2'-O)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + adenosine1067 in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methyladenosine1067 in 23S rRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
adenosine1067 in 23S rRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
2'-O-methyladenosine1067 in 23S rRNA
Comment
The methylase that is responsible for autoimmunity in the thiostrepton producer Streptomyces azureus, renders ribosomes completely resistant to thiostrepton [2].
History
EC 2.1.1.230 created 2011
Orthology
K05926  23S rRNA (adenosine1067-2'-O)-methyltransferase
Genes
CGY: CGLY_05730
CMV: CMUST_02135
STRE: GZL_04335
CFL: Cfla_0863
NAL: B005_2149(nhs)
Taxonomy
Reference
1  [PMID:7925357]
  Authors
Bechthold A, Floss HG
  Title
Overexpression of the thiostrepton-resistance gene from Streptomyces azureus in Escherichia coli and characterization of recognition sites of the 23S rRNA A1067  2'-methyltransferase in the guanosine triphosphatase center of 23S ribosomal RNA.
  Journal
Eur J Biochem 224:431-7 (1994)
DOI:10.1111/j.1432-1033.1994.00431.x
Reference
2  [PMID:6806287]
  Authors
Thompson J, Schmidt F, Cundliffe E
  Title
Site of action of a ribosomal RNA methylase conferring resistance to thiostrepton.
  Journal
J Biol Chem 257:7915-7 (1982)
Reference
3
  Authors
Thompson, J. and Cundliffe, E.
  Title
Purification and properties of an RNA methylase produced by Streptomyces azureus and involved in resistance to thiostrepton.
  Journal
J Gen Microbiol 124:291-297 (1981)
Reference
4  [PMID:20550164]
  Authors
Yang H, Wang Z, Shen Y, Wang P, Jia X, Zhao L, Zhou P, Gong R, Li Z, Yang Y, Chen D, Murchie AI, Xu Y
  Title
Crystal structure of the nosiheptide-resistance methyltransferase of Streptomyces actuosus.
  Journal
Biochemistry 49:6440-50 (2010)
DOI:10.1021/bi1005915
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.230
IUBMB Enzyme Nomenclature: 2.1.1.230
ExPASy - ENZYME nomenclature database: 2.1.1.230
BRENDA, the Enzyme Database: 2.1.1.230

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