KEGG   ENZYME: 2.1.1.244Help
Entry
EC 2.1.1.244                Enzyme                                 

Name
protein N-terminal methyltransferase;
NMT1 (gene name);
METTL11A (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:N-terminal-(A,P,S)PK-[protein] methyltransferase
Reaction(IUBMB)
(1) 3 S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = 3 S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein] (overall reaction);
(1a) S-adenosyl-L-methionine + N-terminal-(A,S)PK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-(A,S)PK-[protein];
(1b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-(A,S)PK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-(A,S)PK-[protein];
(1c) S-adenosyl-L-methionine + N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein] = S-adenosyl-L-homocysteine + N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein];
(2) 2 S-adenosyl-L-methionine + N-terminal-PPK-[protein] = 2 S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein] (overall reaction);
(2a) S-adenosyl-L-methionine + N-terminal-PPK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N-methyl-N-PPK-[protein];
(2b) S-adenosyl-L-methionine + N-terminal-N-methyl-N-PPK-[protein] = S-adenosyl-L-homocysteine + N-terminal-N,N-dimethyl-N-PPK-[protein]
Substrate
S-adenosyl-L-methionine [CPD:C00019];
N-terminal-(A,S)PK-[protein];
N-terminal-N-methyl-N-(A,S)PK-[protein];
N-terminal-N,N-dimethyl-N-(A,S)PK-serine-[protein];
N-terminal-PPK-[protein];
N-terminal-N-methyl-N-PPK-[protein]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N-terminal-N,N,N-trimethyl-N-(A,S)PK-[protein];
N-terminal-N-methyl-N-(A,S)PK-[protein];
N-terminal-N,N-dimethyl-N-(A,S)PK-[protein];
N-terminal-N,N-dimethyl-N-PPK-[protein];
N-terminal-N-methyl-N-PPK-[protein]
Comment
This enzyme methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys after the initiator L-methionine is cleaved. When the terminal amino acid is L-proline, the enzyme catalyses two successive methylations of its alpha-amino group. When the first amino acid is either L-alanine or L-serine, the enzyme catalyses three successive methylations.
The Pro-Lys in positions 2-3 cannot be exchanged for other amino acids [1,2].
History
EC 2.1.1.244 created 2012
Orthology
K16219  protein N-terminal methyltransferase
Genes
HSA: 28989(NTMT1)
PTR: 464790(NTMT1)
PPS: 100987374(NTMT1)
GGO: 101142309(NTMT1)
PON: 100431666(NTMT1)
NLE: 100603188(NTMT1)
MCC: 716763(NTMT1)
MCF: 102126169(NTMT1)
CSAB: 103239762(NTMT1)
RRO: 104660538(NTMT1)
RBB: 108512842(NTMT1)
CJC: 100415418(NTMT1)
SBQ: 101030485(NTMT1)
MMU: 66617(Ntmt1)
RNO: 362103(Ntmt1)
CGE: 100771449(Ntmt1)
NGI: 103727878(Ntmt1)
HGL: 101704679(Ntmt1)
CCAN: 109696680(Ntmt1)
OCU: 100339797(NTMT1)
TUP: 102484497(NTMT1)
CFA: 491300(NTMT1)
AML: 100464977(NTMT1)
UMR: 103670267(NTMT1)
ORO: 101376956(NTMT1)
FCA: 101085326(NTMT1)
PTG: 102970588(NTMT1)
AJU: 106989375(NTMT1)
BTA: 617042(NTMT1)
BOM: 102266812(NTMT1)
BIU: 109566536(NTMT1)
PHD: 102332242(NTMT1)
CHX: 102172454(NTMT1)
OAS: 101115521(NTMT1)
SSC: 100153919(NTMT1)
CFR: 102516861(NTMT1)
CDK: 105092546(NTMT1)
BACU: 103004017(NTMT1)
LVE: 103077988(NTMT1)
OOR: 101286272(NTMT1)
ECB: 100066820(NTMT1)
EPZ: 103551894(NTMT1)
EAI: 106845408(NTMT1)
MYB: 102242208(NTMT1) 106724132
MYD: 102765040(NTMT1)
HAI: 109380490(NTMT1)
RSS: 109445930(NTMT1)
PALE: 102893601(NTMT1)
LAV: 100653829(NTMT1)
TMU: 101360319
MDO: 100012728(NTMT1)
SHR: 100927559(NTMT1) 100931033
GGA: 425539(NTMT1)
MGP: 100549024(NTMT1) 100549986
CJO: 107321904(NTMT1)
APLA: 101791577(NTMT1)
ACYG: 106047278(NTMT1)
TGU: 100222445(NTMT1)
FAB: 101813844(NTMT1)
PHI: 102112016(NTMT1)
PMAJ: 107212070(NTMT1)
CCW: 104688292(NTMT1)
FPG: 101914918(NTMT1)
FCH: 102054150(NTMT1)
CLV: 102084809(NTMT1)
EGZ: 104129807(NTMT1)
ASN: 102385028(NTMT1)
AMJ: 102572961(NTMT1)
PSS: 102449305(NTMT1)
CMY: 102947090(NTMT1)
CPIC: 101942466(NTMT1)
PVT: 110079706(NTMT1)
PBI: 103065843(NTMT1)
GJA: 107109083(NTMT1)
XLA: 734671(ntmt1.S) 735155(ntmt1.L)
XTR: 100145456(ntmt1)
NPR: 108792684(NTMT1)
DRE: 405834(ntmt1)
IPU: 100528268(ntmt1)
AMEX: 103027538(ntmt1)
TRU: 101071402(ntmt1)
LCO: 104926251(ntmt1)
NCC: 104944993
MZE: 101485931(ntmt1)
OLA: 101168668(ntmt1)
XMA: 102223153(ntmt1)
PRET: 103474089(ntmt1)
NFU: 107375044(ntmt1)
CSEM: 103389150(ntmt1)
LCF: 108877709(ntmt1)
HCQ: 109529156(ntmt1)
BPEC: 110161386(ntmt1)
SASA: 106570305(ntmt1)
ELS: 105021779(ntmt1)
SFM: 108922683(ntmt1)
LCM: 102358139(NTMT1)
CMK: 103184690(ntmt1)
CIN: 100185139
SPU: 579562
APLC: 110976444
SKO: 100371905
DME: Dmel_CG1675(Ntmt)
DSI: Dsimw501_GD10692(Dsim_GD10692)
MDE: 101889727
AAG: 5566860
AME: 550780
BIM: 100741318
BTER: 100651146
SOC: 105199772
AEC: 105154167
ACEP: 105622691
PBAR: 105424877
HST: 105190277
CFO: 105250764
LHU: 105679798
PGC: 109858708
NVI: 100121605
TCA: 659517
DPA: 109538561
NVL: 108568005
API: 100167499(Mettl11a)
DNX: 107163768
ZNE: 110833881
FCD: 110846999
TUT: 107362614
CEL: CELE_Y74C9A.3(homt-1)
CBR: CBG03917
BMY: Bm1_20180
TSP: Tsp_08116
CRG: 105321221
MYI: 110451703
OBI: 106875627
EPA: 110254177
HMG: 101236709
AQU: 100636448
ATH: AT5G44450
CRB: 17876613
THJ: 104812070
CPAP: 110821864
CIT: 102626144
TCC: 18595119
GRA: 105777102
DZI: 111275399
EGR: 104441224
VRA: 106752829
VAR: 108321030
CCAJ: 109814486
CAM: 101504939
LJA: Lj4g3v0333650.1(Lj4g3v0333650.1) Lj4g3v0333650.2(Lj4g3v0333650.2)
ADU: 107481711
AIP: 107631571
FVE: 101297996
PPER: 18779269
PMUM: 103325303
PAVI: 110764958
PXB: 103945075
CSV: 101208679
CMO: 103484292
MCHA: 111015851
RCU: 8287902
JCU: 105636784
HBR: 110633462
POP: 7485157
JRE: 108985678
VVI: 104880540
SLY: 101263650
SPEN: 107011688
SOT: 102580009
CANN: 107857634
NSY: 104236734
NTO: 104118695
INI: 109153045
SIND: 105175818
OEU: 111370461
HAN: 110894132
LSV: 111882930
DCR: 108201251
BVG: 104903184
SOE: 110785214
NNU: 104587869
OSA: 4334312
DOSA: Os03t0780900-01(Os03g0780900)
OBR: 102721148
BDI: 100831888
ATS: 109753013(LOC109753013)
SBI: 8083934
ZMA: 100193831
SITA: 101781056
PDA: 103709829
EGU: 105033081
MUS: 103989578
DCT: 110103417
AOF: 109833088
ATR: 18443027
PPP: 112274777
MNG: MNEG_9034
APRO: F751_6105
SCE: YBR261C(TAE1)
ERC: Ecym_2334
KMX: KLMA_50089(TAE1)
NCS: NCAS_0B07330(NCAS0B07330)
NDI: NDAI_0B04650(NDAI0B04650)
TPF: TPHA_0O01360(TPHA0O01360)
TBL: TBLA_0F03410(TBLA0F03410)
TDL: TDEL_0D02090(TDEL0D02090)
KAF: KAFR_0C04340(KAFR0C04340)
CAL: CAALFM_C700490CA(CaO19.7069)
CAUR: QG37_08102
SLB: AWJ20_2262(TAE1)
NCR: NCU01114
NTE: NEUTE1DRAFT76613(NEUTE1DRAFT_76613)
MGR: MGG_05014
SSCK: SPSK_06537
MAW: MAC_08013
MAJ: MAA_03167
BFU: BCIN_03g02980(Bctae1)
MBE: MBM_08281
ANG: ANI_1_940134(An15g06850)
ABE: ARB_05928
TVE: TRV_00128
SPO: SPAC16E8.14c(tae1)
ABP: AGABI1DRAFT35058(AGABI1DRAFT_35058)
ABV: AGABI2DRAFT148576(AGABI2DRAFT_148576)
MGL: MGL_0691
DFA: DFA_02725
EHI: EHI_036850(283.t00004)
SMIN: v1.2.005502.t1(symbB.v1.2.005502.t1) v1.2.010577.t1(symbB.v1.2.010577.t1)
 » show all
Taxonomy
Reference
1  [PMID:20481588]
  Authors
Webb KJ, Lipson RS, Al-Hadid Q, Whitelegge JP, Clarke SG
  Title
Identification of protein N-terminal methyltransferases in yeast and humans.
  Journal
Biochemistry 49:5225-35 (2010)
DOI:10.1021/bi100428x
  Sequence
[hsa:28989] [sce:YBR261C]
Reference
2  [PMID:20668449]
  Authors
Tooley CE, Petkowski JJ, Muratore-Schroeder TL, Balsbaugh JL, Shabanowitz J, Sabat M, Minor W, Hunt DF, Macara IG
  Title
NRMT is an alpha-N-methyltransferase that methylates RCC1 and retinoblastoma protein.
  Journal
Nature 466:1125-8 (2010)
DOI:10.1038/nature09343
  Sequence
[hsa:28989] [mmu:66617]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.244
IUBMB Enzyme Nomenclature: 2.1.1.244
ExPASy - ENZYME nomenclature database: 2.1.1.244
BRENDA, the Enzyme Database: 2.1.1.244

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