KEGG   ENZYME: 2.1.1.257Help
Entry
EC 2.1.1.257                Enzyme                                 

Name
tRNA (pseudouridine54-N1)-methyltransferase;
TrmY;
m1Psi methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNA (pseudouridine54-N1)-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + pseudouridine54 in tRNA = S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
Substrate
S-adenosyl-L-methionine [CPD:C00019];
pseudouridine54 in tRNA
Product
S-adenosyl-L-homocysteine [CPD:C00021];
N1-methylpseudouridine54 in tRNA
Comment
While this archaeal enzyme is specific for the 54 position and does not methylate pseudouridine at position 55, the presence of pseudouridine at position 55 is necessary for the efficient methylation of pseudouridine at position 54 [2,3].
History
EC 2.1.1.257 created 2012
Orthology
K16317  tRNA (pseudouridine54-N1)-methyltransferase
Genes
LUM: CNR27_09035
DTX: ATSB10_14670
XBA: C7S18_10395
VCH: VCA1059
VCF: IR04_04680
VCS: MS6_A1089
VCE: Vch1786_II0744
VCQ: EN18_03535
VCJ: VCD_000281
VCI: O3Y_18448
VVU: VV2_1434
VVY: VVA0268
VPA: VPA0140
VAG: N646_3572
VSP: VS_II1248
VAN: VAA_00947
VSC: VSVS12_04457(trmY)
VTA: B1432
PPR: PBPRA1526(VVA0268)
SFR: Sfri_1778
SBL: Sbal_2539
ABO: ABO_1734
ADI: B5T_04327
APAC: S7S_12770
AXE: P40_21080
BANA: BARAN1_0567(trmY)
MJA: MJ_1640
MMP: MMP0924
MMD: GYY_05340
MAE: Maeo_0669
MVO: Mvol_0946
MFV: Mfer_0333
MKA: MK1018
AFU: AF_1056
FPL: Ferp_0695
GAC: GACE_1384
GAH: GAH_01072
MEAR: Mpt1_c04950(trmY)
MARC: AR505_0759
ABI: Aboo_0918
PHO: PH1401(PH1401)
PAB: PAB1866
PFU: PF1403
PFI: PFC_06240
PYN: PNA2_1979
PYS: Py04_1348
TKO: TK0554
TON: TON_0782
TGA: TGAM_1665
THA: TAM4_1784
THM: CL1_0088
TLT: OCC_08450
THS: TES1_0609
TNU: BD01_0967
TEU: TEU_10635
MAC: MA_1448
MBAR: MSBR2_1010
MBAK: MSBR3_0431
MMA: MM_2534
MMAC: MSMAC_2283
METM: MSMTP_2029
MTHE: MSTHC_0102
MTHR: MSTHT_0618
MHOR: MSHOH_2777
MBU: Mbur_0907
MMET: MCMEM_1261
MMH: Mmah_1033
MPY: Mpsy_2777
MTP: Mthe_0609
MCJ: MCON_2748
MHI: Mhar_0820
MHU: Mhun_3024
MLA: Mlab_1566
MEMA: MMAB1_0381(trmY)
MPI: Mpet_1855
MBN: Mboo_1876
MPL: Mpal_0545
MPD: MCP_2651
MEZ: Mtc_1405
RCI: RCIX2338
HAL: VNG_1980C
HSL: OE_3775R(trmY)
HHB: Hhub_3285(trmY)
HALH: HTSR_0146
HHSR: HSR6_0147(trmY)
HMA: rrnAC3253
HHI: HAH_0499
NPH: NP_2324A(trmY)
NMO: Nmlp_3002(trmY)
HUT: Huta_1107
HTI: HTIA_0957
HMU: Hmuk_1447
HVO: HVO_1989(trmY)
HME: HFX_2082
HLA: Hlac_1588
HTU: Htur_0735
NMG: Nmag_2697(trmY)
NAT: NJ7G_4014
SALI: L593_14300
APE: APE_0797
ACJ: ACAM_0562
IHO: Igni_0291
IIS: EYM_07865
HBU: Hbut_0777
PAI: PAE2175
PIS: Pisl_0819
PCL: Pcal_1090
PAS: Pars_0801
PYR: P186_0414
POG: Pogu_1531
TNE: Tneu_1364
TUZ: TUZN_1182
TTN: TTX_0164a
LOKI: Lokiarch_47640(trmY_1) Lokiarch_49910(trmY_2)
 » show all
Taxonomy
Reference
1  [PMID:21098051]
  Authors
Chen HY, Yuan YA
  Title
Crystal structure of Mj1640/DUF358 protein reveals a putative SPOUT-class RNA methyltransferase.
  Journal
J Mol Cell Biol 2:366-74 (2010)
DOI:10.1093/jmcb/mjq034
  Sequence
[mja:MJ_1640]
Reference
2  [PMID:22274954]
  Authors
Wurm JP, Griese M, Bahr U, Held M, Heckel A, Karas M, Soppa J, Wohnert J
  Title
Identification of the enzyme responsible for N1-methylation of pseudouridine 54 in archaeal tRNAs.
  Journal
RNA 18:412-20 (2012)
DOI:10.1261/rna.028498.111
  Sequence
[hvo:HVO_1989] [mja:MJ_1640]
Reference
3  [PMID:22274953]
  Authors
Chatterjee K, Blaby IK, Thiaville PC, Majumder M, Grosjean H, Yuan YA, Gupta R, de Crecy-Lagard V
  Title
The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at  position 54 of tRNA.
  Journal
RNA 18:421-33 (2012)
DOI:10.1261/rna.030841.111
  Sequence
[hvo:HVO_1989] [mja:MJ_1640]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.257
IUBMB Enzyme Nomenclature: 2.1.1.257
ExPASy - ENZYME nomenclature database: 2.1.1.257
BRENDA, the Enzyme Database: 2.1.1.257

DBGET integrated database retrieval system