KEGG   ENZYME: 2.1.1.258
Entry
EC 2.1.1.258                Enzyme                                 

Name
5-methyltetrahydrofolate---corrinoid/iron-sulfur protein Co-methyltransferase;
acsE (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
5-methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase
Reaction(IUBMB)
a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate = a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate [RN:R02289]
Reaction(KEGG)
R02289;
(other) R10243
Substrate
[methyl-Co(III) corrinoid Fe-S protein];
tetrahydrofolate [CPD:C00101]
Product
[Co(I) corrinoid Fe-S protein];
5-methyltetrahydrofolate [CPD:C00440]
Comment
Catalyses the transfer of a methyl group from the N5 group of methyltetrahydrofolate to the 5-methoxybenzimidazolylcobamide cofactor of a corrinoid/Fe-S protein. Involved, together with EC 1.2.7.4, anaerobic carbon-monoxide dehydrogenase and EC 2.3.1.169, CO-methylating acetyl-CoA synthase, in the reductive acetyl coenzyme A (Wood-Ljungdahl) pathway of autotrophic carbon fixation in various bacteria and archaea.
History
EC 2.1.1.258 created 2012
Pathway
ec00720  Carbon fixation pathways in prokaryotes
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K15023  5-methyltetrahydrofolate corrinoid/iron sulfur protein methyltransferase
Genes
XOR: XOC_1783
XOZ: BE73_08365
DAK: DaAHT2_1991 DaAHT2_2486
DSF: UWK_01140
DOL: Dole_2576 Dole_2853 Dole_3182
DML: Dmul_15220 Dmul_30880(metH) Dmul_32250
DAL: Dalk_0683 Dalk_4619 Dalk_4910
DAT: HRM2_00520(metH1) HRM2_16640(metH3)
DTO: TOL2_C05310 TOL2_C11790(metH)
DOV: DSCO28_10640 DSCO28_24780 DSCO28_59710
SFU: Sfum_2563
BVY: NCTC9239_01829(metH_2)
CLJ: CLJU_c07500(metH2) CLJU_c07550(metH3) CLJU_c20620(metH4) CLJU_c20690(metH5) CLJU_c37560(meTr)
CCE: Ccel_2473
CCEL: CCDG5_0495
ROB: CK5_28650
PDC: CDIF630_00843(acsE)
DAE: Dtox_1276
PTH: PTH_1004(MetH)
HCV: FTV88_3024(metH)
AWO: Awo_c05180(mttA1) Awo_c09210(mttA3) Awo_c10730(acsE) Awo_c15660(mttA4) Awo_c18280(mttA5) Awo_c22740 Awo_c23260(mttB19) Awo_c23750(mttA8)
CTHM: CFE_1661
TKI: TKV_c19830(acsE)
CHY: CHY_1227(acsE)
MTA: Moth_1197
MTHO: MOTHE_c03140(acsE1) MOTHE_c11730(acsE2)
MTHZ: MOTHA_c12600(acsE2)
ADG: Adeg_0341
TPZ: Tph_c01810 Tph_c15130(acsE) Tph_c27600(metH3)
CSC: Csac_0898
ATE: Athe_0749
MANA: MAMMFC1_00471(acsE_1) MAMMFC1_01778(acsE_2) MAMMFC1_02498(acsE_3)
STED: SPTER_03920(acsE_1) SPTER_09890(acsE_2) SPTER_13730(acsE_3) SPTER_28050(acsE_4) SPTER_30460(acsE_6)
OTE: Oter_4334
TTF: THTE_3701
KST: KSMBR1_1319(acsE)
PBU: L21SP3_00049(acsE)
PBP: STSP1_00914(metH_1)
ALUS: STSP2_01509(acsE)
VBL: L21SP4_02015(acsE_1) L21SP4_02433(acsE_2)
RAG: B739_0062
RAT: M949_0493
CTHI: THC_1765
 » show all
Reference
1  [PMID:7928975]
  Authors
Roberts DL, Zhao S, Doukov T, Ragsdale SW
  Title
The reductive acetyl coenzyme A pathway: sequence and heterologous expression of  active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum.
  Journal
J Bacteriol 176:6127-30 (1994)
DOI:10.1128/JB.176.19.6127-6130.1994
  Sequence
[mta:Moth_1197]
Reference
2  [PMID:10997901]
  Authors
Doukov T, Seravalli J, Stezowski JJ, Ragsdale SW
  Title
Crystal structure of a methyltetrahydrofolate- and corrinoid-dependent methyltransferase.
  Journal
Structure 8:817-30 (2000)
DOI:10.1016/S0969-2126(00)00172-6
  Sequence
[mta:Moth_1197]
Reference
3  [PMID:17172470]
  Authors
Doukov TI, Hemmi H, Drennan CL, Ragsdale SW
  Title
Structural and kinetic evidence for an extended hydrogen-bonding network in catalysis of methyl group transfer. Role of an active site asparagine residue in  activation of methyl transfer by methyltransferases.
  Journal
J Biol Chem 282:6609-18 (2007)
DOI:10.1074/jbc.M609828200
  Sequence
[mta:Moth_1197]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.258
IUBMB Enzyme Nomenclature: 2.1.1.258
ExPASy - ENZYME nomenclature database: 2.1.1.258
BRENDA, the Enzyme Database: 2.1.1.258

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