KEGG   ENZYME: 2.1.1.314Help
Entry
EC 2.1.1.314                Enzyme                                 

Name
diphthine methyl ester synthase;
S-adenosyl-L-methionine:elongation factor 2 methyltransferase (ambiguous);
diphthine methyltransferase (ambiguous);
Dph5 (ambiguous)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] methyltransferase (diphthine methyl ester-[translation elongation factor 2]-forming)
Reaction(IUBMB)
4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2] [RN:R11165]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] [CPD:C04441]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
diphthine methyl ester-[translation elongation factor 2] [CPD:C21151]
Comment
This eukaryotic enzyme is part of the biosynthetic pathway of diphthamide. Different from the archaeal enzyme, which performs only 3 methylations, producing diphthine (cf. EC 2.1.1.98). The relevant histidine of elongation factor 2 is His715 in mammals and His699 in yeast. The order of the 4 methylations is not known.
History
EC 2.1.1.314 created 2015
Orthology
K00586  diphthine methyl ester synthase
Genes
HSA: 51611(DPH5)
PTR: 457062(DPH5)
PPS: 100976633(DPH5)
GGO: 101127053(DPH5)
PON: 100453387(DPH5)
NLE: 100586352(DPH5)
MCC: 712253(DPH5)
MCF: 102128354(DPH5)
CSAB: 103224358(DPH5)
RRO: 104673263(DPH5)
RBB: 108519964(DPH5)
CJC: 100415127(DPH5)
SBQ: 101042608(DPH5)
MMU: 69740(Dph5)
RNO: 295394(Dph5)
CGE: 100755934(Dph5)
NGI: 103741876(Dph5)
HGL: 101696986(Dph5)
CCAN: 109698072(Dph5)
OCU: 100345003(DPH5)
TUP: 102477552(DPH5)
CFA: 612092(DPH5)
AML: 100481131(DPH5)
UMR: 103669323(DPH5)
ORO: 101369128(DPH5)
FCA: 101090957(DPH5)
PTG: 102958220(DPH5)
AJU: 106976419(DPH5)
BTA: 508904(DPH5)
BOM: 102267176(DPH5)
BIU: 109556005(DPH5)
PHD: 102335687(DPH5)
CHX: 102173522(DPH5)
OAS: 101115329(DPH5)
SSC: 100157715(DPH5)
CFR: 102518705(DPH5)
CDK: 105084897(DPH5)
BACU: 103000130(DPH5)
LVE: 103089744(DPH5)
OOR: 101285892(DPH5)
ECB: 100050115(DPH5)
EPZ: 103550054(DPH5)
EAI: 106837770(DPH5)
MYB: 102248566(DPH5)
MYD: 102755928(DPH5)
HAI: 109371355(DPH5)
RSS: 109459343(DPH5)
PALE: 102884526(DPH5)
LAV: 100660768(DPH5)
TMU: 101346258
MDO: 100032968(DPH5)
SHR: 100914997(DPH5)
OAA: 100074886(DPH5)
GGA: 424463(DPH5)
MGP: 100549424(DPH5)
CJO: 107317334(DPH5)
APLA: 101793155(DPH5)
ACYG: 106039414(DPH5)
TGU: 100219750(DPH5)
GFR: 102036363(DPH5)
FAB: 101821538(DPH5)
PHI: 102114227(DPH5)
PMAJ: 107208069(DPH5)
CCAE: 111932728(DPH5)
CCW: 104695384(DPH5)
FPG: 101921285(DPH5)
FCH: 102056137(DPH5)
CLV: 102086197(DPH5)
EGZ: 104134012(DPH5)
AAM: 106499746(DPH5)
ASN: 102388223(DPH5)
AMJ: 102573130(DPH5)
PSS: 102462255(DPH5)
CMY: 102931019(DPH5)
CPIC: 101944042(DPH5)
ACS: 100562876(dph5)
PVT: 110076528(DPH5)
PBI: 103049292(DPH5)
GJA: 107107446(DPH5)
XLA: 380263(dph5.L)
XTR: 448611(dph5)
NPR: 108791464(DPH5)
DRE: 492514(dph5)
SRX: 107715097 107757489(dph5)
SANH: 107669825
SGH: 107588067(dph5) 107592713
CCAR: 109047508
IPU: 108271146(dph5)
AMEX: 103037464(dph5)
TRU: 101065858(dph5)
LCO: 104936355(dph5)
NCC: 104954007(dph5)
MZE: 101480932(dph5)
OLA: 101171985(dph5)
XMA: 102236269(dph5)
PRET: 103463161(dph5)
NFU: 107393084(dph5)
KMR: 108248681(dph5)
CSEM: 103398551(dph5)
LCF: 108874223(dph5)
SDU: 111236192(dph5)
HCQ: 109506828(dph5)
MALB: 109965234(dph5)
SASA: 106573967(dph5) 106613590
OTW: 112250700
ELS: 105011378(dph5)
SFM: 108919061(dph5)
LCM: 102345453(DPH5)
CMK: 103188848(dph5)
CIN: 100181968
APLC: 110977432
SKO: 100366716
DME: Dmel_CG31289(Dph5)
DSI: Dsimw501_GD18446(Dsim_GD18446)
DYA: Dyak_GE24024(Dyak_Dph5)
MDE: 101896586
AAG: 5576116
AME: 412557
BIM: 100747247
BTER: 100646946
SOC: 105197794
AEC: 105152429
ACEP: 105626466
PBAR: 105427617
HST: 105184193
DQU: 106750767
CFO: 105254164
LHU: 105668887
PGC: 109857302
PCF: 106790251
NVI: 100120480(DPH5)
MDL: 103572391
DPA: 109537206
NVL: 108567934
BMOR: 101746700
PMAC: 106721558
PRAP: 110997434
HAW: 110373393
PXY: 105382354
API: 100160206
DNX: 107166759
CLEC: 106669101
ZNE: 110831424
FCD: 110859232
TUT: 107360805
CEL: CELE_B0491.7(B0491.7)
CBR: CBG02918
BMY: Bm1_10785
TSP: Tsp_10674
CRG: 105329665
MYI: 110440663
OBI: 106876766
LAK: 106177069
SHX: MS3_04725
EGL: EGR_02199
EPA: 110255079
HMG: 105845166
AQU: 100639400
ATH: AT4G31790
CRB: 17879784
BRP: 103851539
THJ: 104825772
CPAP: 110813746
CIT: 102624591
TCC: 18589955
GRA: 105790074
GHI: 107938393
EGR: 104448830
VRA: 106767046
VAR: 108341879
CCAJ: 109805078
CAM: 101492091
LJA: Lj1g3v2162820.1(Lj1g3v2162820.1) Lj5g3v1807020.1(Lj5g3v1807020.1) Lj5g3v1807020.2(Lj5g3v1807020.2)
LANG: 109338598
FVE: 101310189
PPER: 18792326
PMUM: 103321264
PAVI: 110771090
PXB: 103927928
CSV: 101207207
MCHA: 111013896
CMAX: 111492052
CMOS: 111462364
CPEP: 111806080
RCU: 8274645
JCU: 105639907
HBR: 110673830
POP: 7490655
VVI: 100242000
SLY: 101259434
SPEN: 107015939
SOT: 102593085
CANN: 107866886
NSY: 104231683
NTO: 104104526
INI: 109152797
SIND: 105159337
OEU: 111378944
HAN: 110865884
CCAV: 112502490
DCR: 108215109
BVG: 104897101
SOE: 110791440
NNU: 104588007
OSA: 4332866
DOSA: Os03t0355800-01(Os03g0355800)
OBR: 102714357
BDI: 100846135
ATS: 109767419(LOC109767419)
SBI: 8082642
ZMA: 100191377(pco083348)
SITA: 101779467
PDA: 103719882
EGU: 105044158
MUS: 103976150
DCT: 110104280
PEQ: 110033313
AOF: 109838810
PPP: 112277156
APRO: F751_4143
SCE: YLR172C(DPH5)
ERC: Ecym_2725
KMX: KLMA_40290(DPH5)
NCS: NCAS_0A08800(NCAS0A08800)
NDI: NDAI_0G05220(NDAI0G05220)
TPF: TPHA_0B03580(TPHA0B03580)
TBL: TBLA_0C01150(TBLA0C01150)
TDL: TDEL_0B06200(TDEL0B06200)
KAF: KAFR_0I02070(KAFR0I02070)
PIC: PICST_78373(DPH5)
CAL: CAALFM_C503640WA(CaO19.6676) CAALFM_C503700CA(CaO19.6682)
CAUR: QG37_03403
SLB: AWJ20_4140(DPH5)
NCR: NCU07266
NTE: NEUTE1DRAFT61330(NEUTE1DRAFT_61330)
MGR: MGG_10496
SSCK: SPSK_09544
MAW: MAC_06371
MAJ: MAA_05103
CMT: CCM_04207
BFU: BCIN_11g05290(Bcdph5)
MBE: MBM_01613
ANI: AN0720.2
ANG: ANI_1_1012074(An08g07220)
ABE: ARB_00078
TVE: TRV_00926
PTE: PTT_18731
SPO: SPCC576.14(dph5)
CNE: CNF03650
CNB: CNBF1170
ABP: AGABI1DRAFT56726(AGABI1DRAFT_56726)
ABV: AGABI2DRAFT222176(AGABI2DRAFT_222176)
MGL: MGL_2369
DDI: DDB_G0276115(dph5)
DFA: DFA_02534(dph5)
EHI: EHI_127240(129.t00009)
PYO: PY17X_1210500(PY01561)
PCB: PCHAS_120800(PC000390.05.0)
TAN: TA03050
TPV: TP01_0634
BBO: BBOV_IV002920(21.m02908)
CPV: cgd6_1200
SMIN: v1.2.008542.t1(symbB.v1.2.008542.t1)
SPAR: SPRG_18083
 » show all
Taxonomy
Reference
1  [PMID:3042777]
  Authors
Chen JY, Bodley JW.
  Title
Biosynthesis of diphthamide in Saccharomyces cerevisiae. Partial purification and characterization of a specific S-adenosylmethionine:elongation factor 2 methyltransferase.
  Journal
J Biol Chem 263:11692-6 (1988)
Reference
2  [PMID:3346227]
  Authors
Moehring JM, Moehring TJ.
  Title
The post-translational trimethylation of diphthamide studied in vitro.
  Journal
J Biol Chem 263:3840-4 (1988)
Reference
3  [PMID:24739148]
  Authors
Lin Z, Su X, Chen W, Ci B, Zhang S, Lin H
  Title
Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis.
  Journal
J Am Chem Soc 136:6179-82 (2014)
DOI:10.1021/ja5009272
  Sequence
[sce:YLR172C]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.314
IUBMB Enzyme Nomenclature: 2.1.1.314
ExPASy - ENZYME nomenclature database: 2.1.1.314
BRENDA, the Enzyme Database: 2.1.1.314

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