KEGG   ENZYME: 2.1.1.98Help
Entry
EC 2.1.1.98                 Enzyme                                 

Name
diphthine synthase;
S-adenosyl-L-methionine:elongation factor 2 methyltransferase (ambiguous);
diphthine methyltransferase (ambiguous);
S-adenosyl-L-methionine:2-(3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] methyltransferase;
Dph5 (ambiguous)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] methyltransferase (diphthine-[translation elongation factor 2]-forming)
Reaction(IUBMB)
3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] (overall reaction) [RN:R10306];
(1a) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] [RN:R04481];
(1b) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] [RN:R08468];
(1c) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] [RN:R08469]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] [CPD:C04441];
2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C04692];
2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C16720]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
diphthine-[translation elongation factor 2] [CPD:C01573];
2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C04692];
2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C16720]
Comment
This archaeal enzyme produces the trimethylated product diphthine, which is converted into diphthamide by EC 6.3.1.14, diphthine---ammonia ligase. Different from the eukaryotic enzyme, which produces diphthine methyl ester (cf. EC 2.1.1.314). In the archaeon Pyrococcus horikoshii the enzyme acts on His600 of elongation factor 2.
History
EC 2.1.1.98 created 1990, modified 2013, modified 2015
Orthology
K20215  diphthine synthase
Genes
MJA: MJ_1274
MFE: Mefer_0044
MVU: Metvu_1279
MFS: MFS40622_0657
MIF: Metin_0832
MJH: JH146_0451
MIG: Metig_1174
MMP: MMP0588(dph5)
MMQ: MmarC5_1019
MMX: MmarC6_0305
MMD: GYY_03540
MAE: Maeo_0435
MVO: Mvol_0810
MTH: MTH_1874
MMG: MTBMA_c04450(dphB)
METC: MTCT_1712
MWO: MWSIV6_0405(dphB)
METE: tca_01810
MST: Msp_1017(dphB)
MSI: Msm_0801
MRU: mru_1764(dphB)
MEB: Abm4_1315(dphB)
MMIL: sm9_1721(dphB)
MEYE: TL18_07670
MOL: YLM1_1219
METH: MBMB1_1281(dphB)
MFC: BRM9_0500(dphB)
MFI: DSM1535_0480(dphB)
MCUB: MCBB_1499(dphB)
MFV: Mfer_0700
MKA: MK0747(dph5)
AFU: AF_0381
FPL: Ferp_1481
GAC: GACE_1631
GAH: GAH_00943
TAC: Ta0883
TVO: TVG1034064(TVG1034064)
PTO: PTO1233
FAI: FAD_0348
CDIV: CPM_1291
MEAR: Mpt1_c02170(dphB)
MARC: AR505_1284
ABI: Aboo_0591
PHO: PH0725(PH0725)
PAB: PAB1501
PFU: PF0595
PFI: PFC_02105
PYN: PNA2_1369
PYS: Py04_0739
TKO: TK0106
TON: TON_1372
TGA: TGAM_0314(dph5)
TSI: TSIB_0124
THE: GQS_08695
THA: TAM4_1375
THM: CL1_1363
TLT: OCC_06971
THS: TES1_1349
TNU: BD01_2145
TEU: TEU_05045
PPAC: PAP_06625
MAC: MA_1370(dph5)
MBAR: MSBR2_0974
MBAK: MSBR3_0271
MMA: MM_2355
MMAC: MSMAC_2101
METM: MSMTP_2166
MTHE: MSTHC_2422
MTHR: MSTHT_0849
MHOR: MSHOH_2966
MBU: Mbur_0971
MMET: MCMEM_1474
MMH: Mmah_0645
MPY: Mpsy_3166
MTP: Mthe_1120
MCJ: MCON_1449(dphB)
MHI: Mhar_0531
MHU: Mhun_0788
MLA: Mlab_0386
MBG: BN140_0607(dphB)
MEMA: MMAB1_0789(dphB)
MPI: Mpet_0734
MBN: Mboo_2068
MPL: Mpal_0116
MPD: MCP_0924(dphB)
MEZ: Mtc_2082
RCI: RRC519(dphB)
HAL: VNG_1118G(lds)
HSL: OE_2613R(dph5)
HHB: Hhub_2319(dph5)
HALH: HTSR_1064(dph5)
HHSR: HSR6_1101(dph5)
HMA: rrnAC1406(dph5)
HHI: HAH_1989(dph5)
NPH: NP_1884A(dph5)
NMO: Nmlp_1959(dph5)
HUT: Huta_0243
HTI: HTIA_0426
HMU: Hmuk_1953
HARC: HARCEL1_11525(dph5)
HWA: HQ_1603A(dph5)
HWC: Hqrw_1714(dph5)
HVO: HVO_0916(dph5)
HME: HFX_0887(dph5)
HLA: Hlac_0892
HTU: Htur_2752
NMG: Nmag_0897(dph5)
NAT: NJ7G_1756
SALI: L593_14760
APE: APE_0931(dphB)
ACJ: ACAM_0608(dphB)
SMR: Smar_0509
IHO: Igni_0657
IIS: EYM_06210
DKA: DKAM_1445
TAG: Tagg_1079
IAG: Igag_0048
HBU: Hbut_0128
STO: STK_12780(dphB)
SSO: SSO0953
SOL: Ssol_1929
SSOA: SULA_1961
SSOL: SULB_1962
SSOF: SULC_1960
SAI: Saci_1334
SID: M164_1257
SII: LD85_1385
SIH: SiH_1218
SIR: SiRe_1136
SIC: SiL_1133
MSE: Msed_1735
MCN: Mcup_0493
AHO: Ahos_1400
PAI: PAE1139
PIS: Pisl_1283
PCL: Pcal_0705
PAS: Pars_0230
PYR: P186_2537
POG: Pogu_2233
TNE: Tneu_0216
CMA: Cmaq_1990
TUZ: TUZN_1249
TTN: TTX_0095(dph5)
VDI: Vdis_2028
VMO: VMUT_0435
TPE: Tpen_0689
ASC: ASAC_0414
NMR: Nmar_1297
NCT: NMSP_0391(dphB)
NEV: NTE_01580
NCV: NCAV_1393
NBV: T478_1052(dph5)
NDV: NDEV_1632(dph)
NEQ: NEQ422
NAA: Nps_01115
MARH: Mia14_0002
BARC: AOA65_2396(dphB)
BARB: AOA66_1245(dphB)
 » show all
Taxonomy
Reference
1  [PMID:20873788]
  Authors
Zhu X, Kim J, Su X, Lin H
  Title
Reconstitution of diphthine synthase activity in vitro.
  Journal
Biochemistry 49:9649-57 (2010)
DOI:10.1021/bi100812h
  Sequence
[pho:PH0725]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.98
IUBMB Enzyme Nomenclature: 2.1.1.98
ExPASy - ENZYME nomenclature database: 2.1.1.98
BRENDA, the Enzyme Database: 2.1.1.98
CAS: 114514-25-9

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