KEGG   ENZYME: 2.3.1.165Help
Entry
EC 2.3.1.165                Enzyme                                 

Name
6-methylsalicylic-acid synthase;
MSAS;
6-methylsalicylic acid synthase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl-reducing, thioester-hydrolysing and cyclizing)
Reaction(IUBMB)
acetyl-CoA + 3 malonyl-CoA + NADPH + H+ = 6-methylsalicylate + 4 CoA + 3 CO2 + NADP+ + H2O [RN:R07253]
Reaction(KEGG)
Substrate
acetyl-CoA [CPD:C00024];
malonyl-CoA [CPD:C00083];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Product
6-methylsalicylate [CPD:C02657];
CoA [CPD:C00010];
CO2 [CPD:C00011];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A multienzyme complex with a 4'-phosphopantetheine prosthetic group on the acyl carrier protein. It has a similar sequence to vertebrate type I fatty acid synthase. Acetoacetyl-CoA can also act as a starter molecule.
History
EC 2.3.1.165 created 2002
Pathway
ec01059  Biosynthesis of enediyne antibiotics
ec01130  Biosynthesis of antibiotics
Orthology
K15320  6-methylsalicylic acid synthase
Genes
ELA: UCREL1_5691
PFY: PFICI_12928
GLZ: GLAREA_12216
AOR: AO090206000074
ANG: ANI_1_136174(An10g00140)
AFV: AFLA_114820
ACT: ACLA_093660
PCS: Pc16g00370 Pc22g08170
PDP: PDIP_42600
ABE: ARB_05854
TVE: TRV_06867
PTE: PTT_13075
MMC: Mmcs_3472
MKM: Mkms_3535
MJL: Mjls_3485
MVA: Mvan_3852
MGI: Mflv_2690
MPHL: MPHLCCUG_03492(ppsA_2)
MVQ: MYVA_3673
MTHN: 4412656_02813(ppsA_3)
MJD: JDM601_1527(ppsD)
SALJ: SMD11_6411
SEN: SACE_5308(chlB1)
AOI: AORI_5397(ppsA)
PSEH: XF36_29085
SESP: BN6_39100(pks6-1)
ACTI: UA75_18040
ACAD: UA74_17515
 » show all
Taxonomy
Reference
1  [PMID:1471999]
  Authors
Spencer JB, Jordan PM.
  Title
Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum.
  Journal
Biochem J 288 ( Pt 3):839-46 (1992)
Reference
2  [PMID:8823160]
  Authors
Child CJ, Spencer JB, Bhogal P, Shoolingin-Jordan PM.
  Title
Structural similarities between 6-methylsalicylic acid synthase from Penicillium patulum and vertebrate type I fatty acid synthase: evidence from thiol modification studies.
  Journal
Biochemistry 35:12267-74 (1996)
DOI:10.1021/bi960422e
Reference
3  [PMID:10935930]
  Authors
Richardson MT, Pohl NL, Kealey JT, Khosla C.
  Title
Tolerance and specificity of recombinant 6-methylsalicyclic acid synthase.
  Journal
Metab Eng 1:180-7 (1999)
DOI:10.1006/mben.1999.0113
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.165
IUBMB Enzyme Nomenclature: 2.3.1.165
ExPASy - ENZYME nomenclature database: 2.3.1.165
BRENDA, the Enzyme Database: 2.3.1.165
CAS: 9045-37-8

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