KEGG   ENZYME: 2.3.1.175Help
Entry
EC 2.3.1.175                Enzyme                                 

Name
deacetylcephalosporin-C acetyltransferase;
acetyl-CoA:deacetylcephalosporin-C acetyltransferase;
DAC acetyltransferase;
cefG;
deacetylcephalosporin C acetyltransferase;
acetyl coenzyme A:DAC acetyltransferase;
acetyl-CoA:DAC acetyltransferase;
CPC acetylhydrolase;
acetyl-CoA:DAC O-acetyltransferase;
DAC-AT
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acetyl-CoA:deacetylcephalosporin-C O-acetyltransferase
Reaction(IUBMB)
acetyl-CoA + deacetylcephalosporin C = CoA + cephalosporin C [RN:R03064]
Reaction(KEGG)
Substrate
acetyl-CoA [CPD:C00024];
deacetylcephalosporin C [CPD:C03112]
Product
CoA [CPD:C00010];
cephalosporin C [CPD:C00916]
Comment
This enzyme catalyses the final step in the biosynthesis of cephalosporin C.
History
EC 2.3.1.175 created 2005
Pathway
ec00311  Penicillin and cephalosporin biosynthesis
ec01100  Metabolic pathways
ec01130  Biosynthesis of antibiotics
Orthology
K12747  deacetylcephalosporin-C acetyltransferase
Reference
1  [PMID:1368946]
  Authors
Matsuyama K, Matsumoto H, Matsuda A, Sugiura H, Komatsu K, Ichikawa S.
  Title
Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum.
  Journal
Biosci Biotechnol Biochem 56:1410-2 (1992)
DOI:10.1271/bbb.56.1410
Reference
2  [PMID:1569032]
  Authors
Gutierrez S, Velasco J, Fernandez FJ, Martin JF.
  Title
The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase.
  Journal
J Bacteriol 174:3056-64 (1992)
DOI:10.1128/JB.174.9.3056-3064.1992
  Sequence
Reference
3  [PMID:1632779]
  Authors
Matsuda A, Sugiura H, Matsuyama K, Matsumoto H, Ichikawa S, Komatsu K.
  Title
Cloning and disruption of the cefG gene encoding acetyl coenzyme A: deacetylcephalosporin C o-acetyltransferase from Acremonium chrysogenum.
  Journal
Biochem Biophys Res Commun 186:40-6 (1992)
DOI:10.1016/S0006-291X(05)80772-7
  Sequence
Reference
4  [PMID:9421924]
  Authors
Gutierrez S, Velasco J, Marcos AT, Fernandez FJ, Fierro F, Barredo JL, Diez B, Martin JF.
  Title
Expression of the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum.
  Journal
Appl Microbiol Biotechnol 48:606-14 (1997)
Reference
5  [PMID:9895280]
  Authors
Velasco J, Gutierrez S, Campoy S, Martin JF.
  Title
Molecular characterization of the Acremonium chrysogenum cefG gene product: the native deacetylcephalosporin C acetyltransferase is not processed into subunits.
  Journal
Biochem J 337 ( Pt 3):379-85 (1999)
Reference
6  [PMID:7847890]
  Authors
Martin JF, Gutierrez S, Fernandez FJ, Velasco J, Fierro F, Marcos AT, Kosalkova K.
  Title
Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins.
  Journal
Antonie Van Leeuwenhoek 65:227-43 (1994)
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.175
IUBMB Enzyme Nomenclature: 2.3.1.175
ExPASy - ENZYME nomenclature database: 2.3.1.175
BRENDA, the Enzyme Database: 2.3.1.175
CAS: 57827-76-6

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