KEGG   ENZYME: 2.3.1.182Help
Entry
EC 2.3.1.182                Enzyme                                 

Name
(R)-citramalate synthase;
CimA
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Reaction(IUBMB)
acetyl-CoA + pyruvate + H2O = CoA + (2R)-2-hydroxy-2-methylbutanedioate [RN:R07399]
Reaction(KEGG)
Substrate
acetyl-CoA [CPD:C00024];
pyruvate [CPD:C00022];
H2O [CPD:C00001]
Product
CoA [CPD:C00010];
(2R)-2-hydroxy-2-methylbutanedioate [CPD:C02612]
Comment
One of the enzymes involved in a novel pyruvate pathway for isoleucine biosynthesis that is found in some, mainly archaeal, bacteria [1,2]. The enzyme can be inhibited by isoleucine, the end-product of the pathway, but not by leucine [2]. The enzyme is highly specific for pyruvate as substrate, as the 2-oxo acids 3-methyl-2-oxobutanoate, 2-oxobutanoate, 4-methyl-2-oxopentanoate, 2-oxohexanoate and 2-oxoglutarate cannot act as substrate [1,2].
History
EC 2.3.1.182 created 2007
Pathway
ec00290  Valine, leucine and isoleucine biosynthesis
ec00660  C5-Branched dibasic acid metabolism
ec01100  Metabolic pathways
Orthology
K09011  (R)-citramalate synthase
Genes
MMT: Metme_0870
MDN: JT25_018920
MDH: AYM39_07415
MKO: MKLM6_1526
MAH: MEALZ_1503
CYQ: Q91_0333
CZA: CYCME_2324(leuA2)
CYY: CPC19_11450
TIG: THII_3562
BLEP: AL038_13250
CSS: Cst_c15490(leuA2)
CSD: Clst_1496
LIL: LA_2350(leuA)
LIE: LIF_A1919(leuA)
LIC: LIC_11597(leuA2)
LIS: LIL_11716(leuA2)
LBJ: LBJ_1280(leuA-3)
LBL: LBL_1505(leuA-3)
LBI: LEPBI_I1291(leuA1)
LBF: LBF_1237(leuA-3)
LST: LSS_16681
BTH: BT_1858
BTHO: Btheta7330_01153(leuA_2)
BFR: BF3445
BVU: BVU_2007
BXY: BXY_15010
BOA: Bovatus_01914(leuA_1)
BCEL: BcellWH2_01467(leuA_1)
PMUC: ING2E5A_2804(cimA)
PDI: BDI_2061
TFO: BFO_0823
PSAC: PSM36_0865
APS: CFPG_592
PRU: PRU_0732
AFD: Alfi_1453
ASH: AL1_28190
DORI: FH5T_15045
MBAS: ALGA_0733
PHE: Phep_3427
MUC: MuYL_0828
MGOT: MgSA37_03484(leuA_2)
DFE: Dfer_3672
SLI: Slin_5900
LBY: Lbys_2678
FAE: FAES_4907
PSEZ: HME7025_01306(nifV)
FJO: Fjoh_1305
FJG: BB050_01318(leuA_1)
MARM: YQ22_15945
CBAL: M667_15080
CBAT: M666_15050
ZGA: ZOBELLIA_4558(leuA1)
MLT: VC82_2917
POM: MED152_03680(leuA1)
SPON: HME9304_03288(cimA)
MARF: CJ739_3432
FBU: UJ101_01114(cimA)
MJA: MJ_1392
MMP: MMP1018(cimA)
MMD: GYY_05920
MAE: Maeo_0675
MVO: Mvol_0864
MTH: MTH_723
MMG: MTBMA_c11190(cimA)
METC: MTCT_0645
MWO: MWSIV6_1098(cimA)
METE: tca_00693(leuA_1)
MST: Msp_1318
MSI: Msm_0350
MRU: mru_1414(cimA)
MEB: Abm4_0969(cimA)
MMIL: sm9_0678(cimA)
MEYE: TL18_03265
MOL: YLM1_0912
METH: MBMB1_0337(cimA)
MFC: BRM9_1931(cimA)
MFI: DSM1535_0118(cimA)
MCUB: MCBB_0310(cimA)
MFV: Mfer_0514
MKA: MK0275
AFU: AF_0957
FPL: Ferp_1410
GAC: GACE_1212
GAH: GAH_01414
MAC: MA_3793(leuA)
MBAR: MSBR2_2701
MBAK: MSBR3_2727
MMA: MM_0671
MMAC: MSMAC_0644
METM: MSMTP_0706
MTHE: MSTHC_0928
MTHR: MSTHT_2351
MHOR: MSHOH_0752
MBU: Mbur_0711(cimA)
MMET: MCMEM_1395
MMH: Mmah_0839
MPY: Mpsy_0753
MTP: Mthe_0109
MCJ: MCON_0409(cimA)
MHI: Mhar_1016
MHU: Mhun_1243
MLA: Mlab_0604
MBG: BN140_2289(leuA3)
MEMA: MMAB1_3043(cimA)
MPI: Mpet_0411
MBN: Mboo_1442
MPL: Mpal_2402
MPD: MCP_2701(cimA)
MEZ: Mtc_0210(cimA)
RCI: LRC535(leuA-1)
HALH: HTSR_1495(leuA2)
HHSR: HSR6_1566(leuA2)
HSU: HLASF_0559(leuA1)
HSF: HLASA_0556(leuA1)
HMA: rrnAC1690(leuA1)
HHI: HAH_2225(leuA2)
NPH: NP_0624A(leuA2)
NMO: Nmlp_1234(leuA2)
HUT: Huta_2428
HTI: HTIA_2159(leuA)
HMU: Hmuk_0025
HWA: HQ_1292A(leuA2)
HWC: Hqrw_1326(leuA2)
HVO: HVO_0644(leuA1)
HME: HFX_0617(leuA)
HLA: Hlac_0588
HTU: Htur_2489
NMG: Nmag_0476(leuA2)
NAT: NJ7G_1272
SALI: L593_05815
BARB: AOA66_1694(cimA)
 » show all
Taxonomy
Reference
1  [PMID:9864346]
  Authors
Howell DM, Xu H, White RH.
  Title
(R)-citramalate synthase in methanogenic archaea.
  Journal
J Bacteriol 181:331-3 (1999)
  Sequence
[mja:MJ_1392]
Reference
2  [PMID:15292141]
  Authors
Xu H, Zhang Y, Guo X, Ren S, Staempfli AA, Chiao J, Jiang W, Zhao G.
  Title
Isoleucine biosynthesis in Leptospira interrogans serotype lai strain 56601 proceeds via a threonine-independent pathway.
  Journal
J Bacteriol 186:5400-9 (2004)
DOI:10.1128/JB.186.16.5400-5409.2004
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.182
IUBMB Enzyme Nomenclature: 2.3.1.182
ExPASy - ENZYME nomenclature database: 2.3.1.182
BRENDA, the Enzyme Database: 2.3.1.182

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