KEGG   ENZYME: 2.3.1.189Help
Entry
EC 2.3.1.189                Enzyme                                 

Name
mycothiol synthase;
MshD
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
acetyl-CoA:desacetylmycothiol O-acetyltransferase
Reaction(IUBMB)
desacetylmycothiol + acetyl-CoA = CoA + mycothiol [RN:R09622]
Reaction(KEGG)
Substrate
desacetylmycothiol [CPD:C19703];
acetyl-CoA [CPD:C00024]
Product
CoA [CPD:C00010];
mycothiol [CPD:C06717]
Comment
This enzyme catalyses the last step in the biosynthesis of mycothiol, the major thiol in most actinomycetes, including Mycobacterium [1]. The enzyme is a member of a large family of GCN5-related N-acetyltransferases (GNATs) [2]. The enzyme has been purified from Mycobacterium tuberculosis H37Rv. Acetyl-CoA is the preferred CoA thioester but propionyl-CoA is also a substrate [3].
History
EC 2.3.1.189 created 2010
Orthology
K15520  mycothiol synthase
Genes
MSD: MYSTI_06535
MBD: MEBOL_002064
HOH: Hoch_0457
BRK: CWS35_03445
PBD: PBOR_01460
PGM: PGRAT_12155
PAEN: P40081_13025
PAEA: R70723_11675
PRI: PRIO_0403
TAB: CIG75_08965
MTU: Rv0819(mshD)
MTV: RVBD_0819
MTC: MT0841
MRA: MRA_0829
MTUR: CFBS_0860
MTD: UDA_0819
MTUB: MT7199_0838(mshD)
MTUC: J113_05750
MTUE: J114_04360
MTUL: TBHG_00808
MTUT: HKBT1_0861
MTUU: HKBT2_0861
MBO: BQ2027_MB0842(mshD)
MBB: BCG_0871
MBT: JTY_0841
MBX: BCGT_0619
MAF: MAF_08290
MMIC: RN08_0915
MCQ: BN44_10893(mshD)
MCV: BN43_20262(mshD)
MCX: BN42_20581(mshD)
MCZ: BN45_20088(mshD)
MLE: ML2193
MLB: MLBr02193
MPA: MAP_0650
MAO: MAP4_3216
MAVI: RC58_15955
MAVU: RE97_15985
MAV: MAV_0761
MAVD: NF84_03485
MAVR: LA63_03575
MAVA: LA64_03585
MIT: OCO_06660
MIA: OCU_06710
MID: MIP_01176
MYO: OEM_06750
MIR: OCQ_06810
MMAL: CKJ54_03265(mshD)
MLP: MLM_0882
MUL: MUL_0436(mshD)
MMC: Mmcs_4524
MKM: Mkms_4611
MJL: Mjls_4907
MMI: MMAR_4864(mshD)
MMAE: MMARE11_46720(mshD)
MMM: W7S_03275
MLI: MULP_05097(mshD)
MHAD: B586_05445
MVA: Mvan_5096
MGI: Mflv_1651
MFT: XA26_10530(mshD)
MPHL: MPHLCCUG_00716(mshD_1)
MVQ: MYVA_4948
MTHN: 4412656_03920(mshD)
MAB: MAB_0745
MABB: MASS_0714
MABO: NF82_03750
MCHE: BB28_03585
MSTE: MSTE_00704(mshD)
MJD: JDM601_3540(mshD)
MTER: 4434518_03510(mshD)
ASD: AS9A_0748(mshD)
CGL: NCgl2487(Cgl2576)
CGB: cg2847
CGU: WA5_2487(MshD)
CGT: cgR_2479
CGM: cgp_2847(mshD)
CGJ: AR0_12395
CEF: CE2468
CDI: DIP1911
CDP: CD241_1822(mshD)
CDH: CDB402_1777(mshD)
CDT: CDHC01_1824(mshD)
CDE: CDHC02_1812(mshD)
CDR: CDHC03_1793(mshD)
CDA: CDHC04_1796(mshD)
CDZ: CD31A_1907(mshD)
CDB: CDBH8_1883(mshD)
CDS: CDC7B_1881(mshD)
CDW: CDPW8_1878(mshD)
CDV: CDVA01_1757(mshD)
CDIP: ERS451417_01932(mshD)
CJK: jk0376
CUR: cu1609
CUA: CU7111_1550(mshD)
CAR: cauri_2155(mshD)
CPU: cpfrc_01714(mshD)
CPL: Cp3995_1763(mshD)
CPG: Cp316_1767(mshD)
CPP: CpP54B96_1744(mshD)
CPK: Cp1002_1715(mshD)
CPQ: CpC231_1707(mshD)
CPX: CpI19_1723(mshD1)
CPZ: CpPAT10_1715(mshD)
COR: Cp267_1784(mshD)
COP: Cp31_1706(mshD2)
COD: Cp106_1672(mshD)
COS: Cp4202_1704(mshD)
COI: CpCIP5297_1732(mshD2)
COE: Cp258_1731(mshD)
COU: Cp162_1692(mshD2)
CPSE: CPTA_00093
CPSU: CPTB_01434
CPSF: CPTC_01104
CRD: CRES_0111(mshD)
CUL: CULC22_01890(mshD)
CUC: CULC809_01788(mshD)
CUE: CULC0102_1931(mshD)
CUN: Cul210932_1905(mshD2)
CUS: CulFRC11_1801(mshD2)
CUQ: Cul210931_1799(mshD2)
CUZ: Cul05146_1896(mshD2)
CUJ: CUL131002_1823c(mshD2)
CVA: CVAR_0578(mshD)
CTER: A606_02605
CGY: CGLY_13040(mshD)
COA: DR71_697(mshD)
CSX: CSING_11145(mshD)
CMQ: B840_10550(mshD)
CKU: UL82_02875(mshD)
CCJ: UL81_09640(mshD)
CMV: CMUST_12905(mshD)
CEI: CEPID_10370(mshD)
CTED: CTEST_11175(mshD)
CUT: CUTER_09195(mshD)
CDX: CDES_11710(mshD)
CSP: WM42_1860(mshD)
CPHO: CPHO_02500
CGV: CGLAU_10450(mshD2)
CAQU: CAQU_10385
CAMG: CAMM_10850
CMIN: NCTC10288_00413(mshD)
NFA: NFA_5990(mshD)
NFR: ERS450000_03621(mshD)
NSR: NS506_01398(mshD)
NTP: CRH09_03895(mshD)
RER: RER_48050(mshD)
REY: O5Y_22760
ROP: ROP_49430(mshD)
REQ: REQ_08610(mshD)
RHB: NY08_2607
RFA: A3L23_00766(mshD_2)
RHS: A3Q41_02586(mshD_5)
RRZ: CS378_20800(mshD)
RHU: A3Q40_02787(mshD_5)
RQI: C1M55_24490(mshD)
RRT: 4535765_00796(mshD)
GBR: Gbro_3934
GPO: GPOL_c40580(mshD)
GOC: CXX93_06775(mshD)
GIT: C6V83_04540(mshD)
TPR: Tpau_3544
SRT: Srot_3047
DIT: C3V38_09800(mshD)
DIZ: CT688_03730(mshD)
SCO: SCO4151(SCD84.18c)
SMA: SAVERM_4058(mshD)
SGR: SGR_3940
SGB: WQO_15905
SCT: SCAT_2559(mshD)
SFA: Sfla_2896
SBH: SBI_05018
SHY: SHJG_4932
SVE: SVEN_3908
SDV: BN159_4782(mshD)
SALB: XNR_3309
SALS: SLNWT_4051
STRP: F750_3895(mshD)
SFI: SFUL_3927
SALU: DC74_4724
SALL: SAZ_25195
SLV: SLIV_17620(mshD)
SGU: SGLAU_15955(mshD)
STRE: GZL_04118
SLD: T261_3527
STRM: M444_16600
SAMB: SAM23877_3532(mshD)
SPRI: SPRI_4005
SRW: TUE45_04686(mshD_4)
SLE: sle_38040(sle_38040)
SRN: A4G23_02540(mshD_3)
SNR: SNOUR_18295(mshD)
STRD: NI25_21135
SMAL: SMALA_3540
SLAU: SLA_4003
SALJ: SMD11_2907
SLX: SLAV_17920(mshD3)
SFK: KY5_3802
KSK: KSE_43360(mshD)
STRI: C7M71_013525(mshD)
CMI: CMM_2502
CMS: CMS2730
CMC: CMN_02455(mshD)
CRY: B7495_13650(mshD)
MYL: C3E77_10890(mshD)
SALC: C2138_01545(mshD)
HUM: DVJ78_15600(mshD)
ART: Arth_3013
ARM: ART_2163
ARN: CGK93_17010(mshD)
ARX: ARZXY2_688(mshD)
ARTH: C3B78_14420(mshD)
AAU: AAur_2987
ACH: Achl_2725
KRH: KRH_05710(mshD)
RDN: HMPREF0733_10355(mshD)
AUL: DCC27_000195(mshD)
BCV: Bcav_3429
BRV: CFK39_05370(mshD)
BGG: CFK41_03580(mshD)
BRZ: CFK38_05715(mshD)
BSAU: DWV08_10610(mshD)
JDE: Jden_2234
LMOI: VV02_07155
XCE: Xcel_0316
IDO: I598_1982(mshD_4)
CFL: Cfla_2814
SERJ: SGUI_2593
ORN: DV701_08570(mshD)
BLIN: BLSMQ_3335
PFRE: RM25_1900
PPC: HMPREF9154_0459(mshD)
MPH: MLP_47690(mshD)
NCA: Noca_4248
NDK: I601_1724(mshD_2)
KFL: Kfla_0970
PSIM: KR76_22915
AEZ: C3E78_02515(mshD)
MGG: MPLG2_3704(mshD)
TFU: Tfu_2739
NDA: Ndas_4378
NAL: B005_1560(mshD)
NGV: CDO52_04525(mshD)
TCU: Tcur_0594
SRO: Sros_0893
FAL: FRAAL0935
ACE: Acel_2026
NML: Namu_4982
GOB: Gobs_4647
BSD: BLASA_4534(mshD)
MMAR: MODMU_5050(mshD)
KRA: Krad_0878
SEN: SACE_7100(mshD)
AMD: AMED_9007
AMN: RAM_46200
AMM: AMES_8871
AMZ: B737_8872
AOI: AORI_7750
AJA: AJAP_40445(mshD)
AMQ: AMETH_3199(mshD) AMETH_6921(mshD)
AMYC: CU254_37495(mshD)
PDX: Psed_0460
AMI: Amir_6822
APRE: CNX65_33740(mshD)
SESP: BN6_81960(mshD)
KAL: KALB_8511
ACTI: UA75_29645
ACAD: UA74_29115
AHG: AHOG_26885(mshD7)
ACTA: C1701_12185(mshD)
ALL: CRK60405
SAQ: Sare_0335
MIL: ML5_2774
ASE: ACPL_7972
ACTN: L083_7756
ACTS: ACWT_7841
PLK: CIK06_26435(mshD)
PLAB: C6361_11175(mshD)
PLAT: C6W10_08270(mshD)
CAI: Caci_8254
SNA: Snas_6377
TBI: Tbis_0451
AEY: CDG81_21635(mshD)
ABAM: B1s21122_05850(mshD)
AFO: Afer_1462
AYM: YM304_22590(mshD)
PLH: VT85_25145(mshD)
PBOR: BSF38_03408(mshD_4)
BARC: AOA65_1748
 » show all
Taxonomy
Reference
1  [PMID:8076641]
  Authors
Spies HS, Steenkamp DJ
  Title
Thiols of intracellular pathogens. Identification of ovothiol A in Leishmania donovani and structural analysis of a novel thiol from Mycobacterium bovis.
  Journal
Eur J Biochem 224:203-13 (1994)
DOI:10.1111/j.1432-1033.1994.tb20013.x
Reference
2  [PMID:12375100]
  Authors
Koledin T, Newton GL, Fahey RC
  Title
Identification of the mycothiol synthase gene (mshD) encoding the acetyltransferase producing mycothiol in actinomycetes.
  Journal
Arch Microbiol 178:331-7 (2002)
DOI:10.1007/s00203-002-0462-y
  Sequence
Reference
3  [PMID:12930994]
  Authors
Vetting MW, Roderick SL, Yu M, Blanchard JS
  Title
Crystal structure of mycothiol synthase (Rv0819) from Mycobacterium tuberculosis  shows structural homology to the GNAT family of N-acetyltransferases.
  Journal
Protein Sci 12:1954-9 (2003)
DOI:10.1110/ps.03153703
  Sequence
[mtu:Rv0819]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.189
IUBMB Enzyme Nomenclature: 2.3.1.189
ExPASy - ENZYME nomenclature database: 2.3.1.189
BRENDA, the Enzyme Database: 2.3.1.189

DBGET integrated database retrieval system