KEGG   ENZYME: 2.3.1.65Help
Entry
EC 2.3.1.65                 Enzyme                                 

Name
bile acid-CoA:amino acid N-acyltransferase;
glycine---taurine N-acyltransferase;
amino acid N-choloyltransferase;
BAT;
glycine N-choloyltransferase;
BACAT;
cholyl-CoA glycine-taurine N-acyltransferase;
cholyl-CoA:taurine N-acyltransferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
choloyl-CoA:glycine N-choloyltransferase
Reaction(IUBMB)
choloyl-CoA + glycine = CoA + glycocholate [RN:R03718]
Reaction(KEGG)
Substrate
choloyl-CoA [CPD:C01794];
glycine [CPD:C00037]
Product
CoA [CPD:C00010];
glycocholate [CPD:C01921]
Comment
Also acts on CoA derivatives of other bile acids. Taurine and 2-fluoro-beta-alanine can act as substrates, but more slowly [4]. The enzyme can also conjugate fatty acids to glycine and can act as a very-long-chain acyl-CoA thioesterase [7]. Bile-acid---amino-acid conjugates serve as detergents in the gastrointestinal tract, solubilizing long chain fatty acids, mono- and diglycerides, fat-soluble vitamins and cholesterol [4]. This is the second enzyme in a two-step process leading to the conjugation of bile acids with amino acids; the first step is the conversion of bile acids into their acyl-CoA thioesters, which is catalysed by EC 6.2.1.7, cholate---CoA ligase.
History
EC 2.3.1.65 created 1983, modified 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec00430  Taurine and hypotaurine metabolism
ec01100  Metabolic pathways
Orthology
K00659  bile acid-CoA:amino acid N-acyltransferase
Genes
HSA: 570(BAAT)
PTR: 472997
PPS: 100972861 100992429(BAAT)
GGO: 101140183(BAAT)
PON: 100431907(BAAT)
NLE: 100598200(BAAT)
MCC: 100429419(BAAT)
MCF: 102132073(BAAT) 107127621
CSAB: 103219118(BAAT)
RRO: 104670797(BAAT)
RBB: 108537176(BAAT)
CJC: 100394190 100396712(BAAT)
MMU: 12012(Baat)
RNO: 29725(Baat)
CGE: 100756252(Baat) 100756541
HGL: 101716142(Baat) 101717293
CCAN: 109696243(Baat)
TUP: 102472797 102473647(BAAT)
CFA: 481635
AML: 100476252(BAAT)
FCA: 101095691(BAAT) 101096116
PTG: 102969591(BAAT) 102969870
AJU: 106972069(BAAT) 106972070
BTA: 530653 786798(BAAT)
BOM: 102268176(BAAT) 102268462
BIU: 109563300(BAAT)
PHD: 102325859 102326210(BAAT)
CHX: 102174570(BAAT)
CFR: 102523415(BAAT)
CDK: 105086911(BAAT)
BACU: 103006095 103017253(BAAT)
LVE: 103081315 103082496(BAAT)
OOR: 101284164(BAAT) 101284403
ECB: 100054567 100063137(BAAT)
EPZ: 103552469(BAAT) 103568036
HAI: 109395040(BAAT)
RSS: 109446124(BAAT)
PALE: 102892341(BAAT) 102897591
TMU: 101357199
MDO: 100023136(BAAT)
GGA: 769879(BAAT)
MGP: 100539658(BAAT)
CJO: 107314177
APLA: 101797844
ACYG: 106048282
GFR: 102037789
FAB: 101815920
PHI: 102101793
PMAJ: 107206148
CCAE: 111930612
FPG: 101924136
FCH: 102059453
CLV: 102094683
EGZ: 104134405(BAAT)
AAM: 106494986
CPIC: 101932563 101938826(BAAT)
ACS: 100566890(baat)
PVT: 110078385(BAAT)
PBI: 103065567(BAAT)
GJA: 107106123 107106126(BAAT)
XLA: 100137716(LOC100488333.L) 108707337
SRX: 107726829
SANH: 107689257
IPU: 108280978
OLA: 101166298
XMA: 102226402
PRET: 103465285
NFU: 107385610
SDU: 111221490
HCQ: 109522368
ELS: 105020184
CIN: 100175018
CRG: 105343873
MYI: 110465077
OBI: 106881808
ADF: 107346883
 » show all
Taxonomy
Reference
1  [PMID:7372637]
  Authors
Czuba B, Vessey DA.
  Title
Kinetic characterization of cholyl-CoA glycine-taurine N-acyltransferase from bovine liver.
  Journal
J Biol Chem 255:5296-9 (1980)
Reference
2
  Authors
Jordan, T.W., Lee, R. and Lim, W.C.
  Title
Isoelectric focussing of soluble and particulate benzoyl-CoA and cholyl-CoA:amino acid N-acyltransferases from rat liver.
  Journal
Biochem Int 1:325-330 (1980)
Reference
3  [PMID:422567]
  Authors
Vessey DA.
  Title
The co-purification and common identity of cholyl CoA:glycine- and cholyl CoA:taurine-N-acyltransferase activities from bovine liver.
  Journal
J Biol Chem 254:2059-63 (1979)
Reference
4  [PMID:2037576]
  Authors
Johnson MR, Barnes S, Kwakye JB, Diasio RB.
  Title
Purification and characterization of bile acid-CoA:amino acid N-acyltransferase from human liver.
  Journal
J Biol Chem 266:10227-33 (1991)
  Sequence
[hsa:570]
Reference
5  [PMID:12454267]
  Authors
Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S.
  Title
Molecular cloning and expression of rat liver bile acid CoA ligase.
  Journal
J Lipid Res 43:2062-71 (2002)
Reference
6  [PMID:12951368]
  Authors
He D, Barnes S, Falany CN.
  Title
Rat liver bile acid CoA:amino acid N-acyltransferase: expression, characterization, and peroxisomal localization.
  Journal
J Lipid Res 44:2242-9 (2003)
DOI:10.1194/jlr.M300128-JLR200
  Sequence
[rno:29725]
Reference
7  [PMID:12810727]
  Authors
O'Byrne J, Hunt MC, Rai DK, Saeki M, Alexson SE.
  Title
The human bile acid-CoA:amino acid N-acyltransferase functions in the conjugation of fatty acids to glycine.
  Journal
J Biol Chem 278:34237-44 (2003)
DOI:10.1074/jbc.M300987200
  Sequence
[hsa:570]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.65
IUBMB Enzyme Nomenclature: 2.3.1.65
ExPASy - ENZYME nomenclature database: 2.3.1.65
BRENDA, the Enzyme Database: 2.3.1.65
CAS: 65979-40-0

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