KEGG   ENZYME: 2.3.1.97Help
Entry
EC 2.3.1.97                 Enzyme                                 

Name
glycylpeptide N-tetradecanoyltransferase;
NMT (gene name);
peptide N-myristoyltransferase;
myristoyl-CoA-protein N-myristoyltransferase;
myristoyl-coenzyme A:protein N-myristoyl transferase;
myristoylating enzymes;
protein N-myristoyltransferase;
tetradecanoyl-CoA:glycylpeptide N-tetradecanoyltransferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
BRITE hierarchy
Sysname
tetradecanoyl-CoA:N-terminal-glycine-[protein] N-tetradecanoyltransferase
Reaction(IUBMB)
tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein] [RN:R03992]
Reaction(KEGG)
Substrate
tetradecanoyl-CoA [CPD:C02593];
N-terminal-glycyl-[protein] [CPD:C02038]
Product
CoA [CPD:C00010];
N-terminal-N-tetradecanoylglycyl-[protein] [CPD:C03881]
Comment
The enzyme catalyses the transfer of myristic acid from myristoyl-CoA to the amino group of the N-terminal glycine residue in a variety of eukaryotic proteins. It uses an ordered Bi Bi reaction in which myristoyl-CoA binds to the enzyme prior to the binding of the peptide substrate, and CoA release precedes the release of the myristoylated peptide. The enzyme from yeast is profoundly affected by amino acids further from the N-terminus, and is particularly stimulated by a serine residue at position 5.
History
EC 2.3.1.97 created 1989, modified 1990, modified 2018
Orthology
K00671  glycylpeptide N-tetradecanoyltransferase
Genes
HSA: 4836(NMT1) 9397(NMT2)
PTR: 450321(NMT2) 454744(NMT1)
PPS: 100972009(NMT2) 100981120(NMT1)
GGO: 101132237(NMT2) 101143947(NMT1)
PON: 100174462(NMT1) 100438396(NMT2)
NLE: 100602350(NMT2) 100604682(NMT1)
MCC: 703394(NMT2) 715885(NMT1)
MCF: 102132842(NMT1) 102143732(NMT2)
CSAB: 103237937(NMT2) 103243298(NMT1)
RRO: 104654735(NMT1) 104664161 104664481(NMT2)
RBB: 108530958(NMT2) 108532210(NMT1)
CJC: 100394723(NMT1) 100403561(NMT2)
SBQ: 101044627(NMT2) 101048199(NMT1)
MMU: 18107(Nmt1) 18108(Nmt2)
RNO: 259274(Nmt1) 291318(Nmt2)
CGE: 100756019(Nmt2) 100759338(Nmt1)
NGI: 103735179(Nmt2) 103738312(Nmt1)
HGL: 101697570(Nmt2) 101706184(Nmt1)
CCAN: 109691355(Nmt2) 109691569(Nmt1)
OCU: 100340182(NMT1) 100348670(NMT2)
TUP: 102494402(NMT1) 102494701(NMT2)
CFA: 480494(NMT1) 487120(NMT2)
AML: 100465245(NMT1) 100479016(NMT2)
UMR: 103664240(NMT2) 103665877(NMT1)
ORO: 101365018(NMT1) 101376568(NMT2)
FCA: 101082703(NMT1) 101090271(NMT2)
PTG: 102957537(NMT1) 102963277(NMT2)
AJU: 106970997(NMT2) 106988151(NMT1)
BTA: 281351(NMT1) 282049(NMT2)
BOM: 102269914(NMT1) 102273686(NMT2) 102276941
BIU: 109573525(NMT1)
PHD: 102324029(NMT1) 102324080(NMT2)
CHX: 102180607(NMT2) 102187801(NMT1)
OAS: 101117182(NMT2) 101117775(NMT1) 101122794
SSC: 100524705(NMT1) 100627249(NMT2)
CFR: 102503609(NMT1) 102522093(NMT2)
CDK: 105094410(NMT1) 105104530(NMT2)
BACU: 103017535(NMT1) 103021054(NMT2)
LVE: 103080943(NMT1) 103083512(NMT2)
OOR: 101271335(NMT1) 101280336(NMT2)
ECB: 100050277(NMT1) 100068766(NMT2)
EPZ: 103554678(NMT1) 103556819(NMT2)
EAI: 106836473(NMT1) 106840449(NMT2)
MYB: 102239257(NMT2) 102259380(NMT1)
MYD: 102754083 102759111(NMT2) 102760509(NMT1)
HAI: 109393804(NMT2) 109394977(NMT1)
RSS: 109441220 109450705(NMT2) 109458500(NMT1)
PALE: 102895637(NMT2) 102896428(NMT1)
LAV: 100661077(NMT1) 100675378(NMT2)
MDO: 100020457(NMT1) 100020704(NMT2)
SHR: 100914067(NMT2) 100916560(NMT1)
OAA: 100077553(NMT2)
GGA: 419966(NMT1) 420529(NMT2)
MGP: 100546804(NMT2) 100550671(NMT1)
CJO: 107309020(NMT2) 107325008(NMT1)
APLA: 101795652(NMT1) 101796368(NMT2)
ACYG: 106039667(NMT2) 106046932(NMT1)
TGU: 100218058(NMT2) 100226494(NMT1)
GFR: 102037477(NMT2) 102038594(NMT1)
FAB: 101808830(NMT1) 101819865(NMT2)
PHI: 102109880(NMT2) 102113086(NMT1)
PMAJ: 107199973(NMT2) 107215290(NMT1)
CCW: 104689202(NMT2) 104698535(NMT1)
FPG: 101919223(NMT2) 101919935(NMT1)
FCH: 102048284(NMT1) 102058808(NMT2)
CLV: 102084902(NMT2) 102087955(NMT1)
EGZ: 104128742(NMT2) 104132241(NMT1)
AAM: 106482872 106483500(NMT2) 106495648(NMT1)
ASN: 102385616(NMT2) 102386761(NMT1)
AMJ: 102576715(NMT1) 106736799(NMT2)
PSS: 102462346(NMT2)
CMY: 102931451(NMT2) 102940056(NMT1)
CPIC: 101933923(NMT1) 101946890(NMT2)
ACS: 100555077(nmt2) 100559200(nmt1)
PVT: 110070294(NMT2) 110081387(NMT1)
PBI: 103052935(NMT1) 103065880(NMT2)
GJA: 107120602(NMT1) 107120952(NMT2)
XLA: 108702554 108706069 108719884 379884(nmt2.L) 444545(nmt1.L)
XTR: 100380009(nmt2) 779719(nmt1)
NPR: 108785979(NMT1) 108795076(NMT2)
DRE: 323956(nmt1a) 556483(nmt2)
IPU: 108256512(nmt2) 108260599(nmt1) 108273814
AMEX: 103042142(nmt2) 103044353(nmt1) 103045678
TRU: 101065497 101075895(nmt2) 101076413(nmt1)
LCO: 104923046(nmt2) 104926429(nmt1) 104930723
NCC: 104946858(nmt1) 104953571 104959140(nmt2)
XMA: 102218082(nmt1) 102227956 102228322(nmt2)
PRET: 103469337(nmt1) 103478683(nmt2) 103481311
NFU: 107378558(nmt1) 107379648(nmt2) 107396746
LCF: 108872771(nmt1) 108873429 108889590(nmt2)
HCQ: 109506793(nmt1) 109523896 109528980(nmt2)
BPEC: 110159280(nmt1) 110163117(nmt2) 110171043
LCM: 102346969(NMT1) 102357978(NMT2)
CMK: 103187068(nmt2) 103189700(nmt1)
CIN: 100181870
APLC: 110973435
SKO: 100378935
DME: Dmel_CG7436(Nmt)
DSI: Dsimw501_GD13014(Dsim_GD13014)
MDE: 101900950
AAG: 5565981
AME: 552485
BIM: 100749273
BTER: 100652054
SOC: 105196777
AEC: 105148711
ACEP: 105623311
PBAR: 105426306
HST: 105188714
CFO: 105251111
LHU: 105669889
PGC: 109854928
NVI: 100115755
TCA: 661933
DPA: 109542013
NVL: 108560391
BMOR: 101735807
PMAC: 106708997
PRAP: 111003236
PXY: 105389467
API: 100163432
DNX: 107169641
ZNE: 110837394
FCD: 110845885
TUT: 107368114
CEL: CELE_T17E9.2(nmt-1)
CBR: CBG21106(Cbr-nmt-1)
BMY: Bm1_22900
TSP: Tsp_00547
CRG: 105326790
MYI: 110457073
OBI: 106875636
LAK: 106162072
SHX: MS3_09237
EGL: EGR_04495
EPA: 110254670
ADF: 107329364
AQU: 100637114
ATH: AT5G57020(NMT1)
CPAP: 110816817
LJA: Lj3g3v2664340.1(Lj3g3v2664340.1) Lj6g3v1226280.1(Lj6g3v1226280.1)
ADU: 107464435
AIP: 107618291
FVE: 101304413
CSV: 101203401
CMO: 103492341
MCHA: 111016535
BVG: 104888039
SOE: 110775579
OSA: 4327681
DOSA: Os01t0708100-01(Os01g0708100)
OBR: 102704564
ATS: 109744913(LOC109744913)
SBI: 8059165
SITA: 101777188
AOF: 109833399
CRE: CHLREDRAFT_97790(NMT1)
MNG: MNEG_5641
MIS: MICPUN_58555(nmt)
APRO: F751_3117
SCE: YLR195C(NMT1)
ERC: Ecym_4716
KMX: KLMA_50308(NMT1)
NCS: NCAS_0E02830(NCAS0E02830)
NDI: NDAI_0E04430(NDAI0E04430)
TPF: TPHA_0F03040(TPHA0F03040)
TBL: TBLA_0C05020(TBLA0C05020)
TDL: TDEL_0C01910(TDEL0C01910)
KAF: KAFR_0L01530(KAFR0L01530)
PIC: PICST_80771(NMT1)
CAL: CAALFM_C401440WA(NMT1)
CDU: CD36_41450(NMT1)
CAUR: QG37_01962
SLB: AWJ20_4856(NMT1)
NCR: NCU17177
NTE: NEUTE1DRAFT123655(NEUTE1DRAFT_123655)
MGR: MGG_07229
SSCK: SPSK_06150
MAW: MAC_08789
MAJ: MAA_08962
BFU: BCIN_01g02420(Bcnmt1)
MBE: MBM_00920
ANI: AN3839.2
ANG: ANI_1_508184(An04g02500)
PTE: PTT_14787
CNE: CNN00080
CNB: CNBN0070
ABP: AGABI1DRAFT34763(AGABI1DRAFT_34763)
ABV: AGABI2DRAFT148548(AGABI2DRAFT_148548)
MGL: MGL_0604
DDI: DDB_G0275863(nmt)
DFA: DFA_10733(nmt)
EHI: EHI_159730(88.t00002)
PYO: PY17X_1032200(PY01548)
PCB: PCHAS_103060(PC300802.00.0)
TAN: TA13465
TPV: TP02_0057
BBO: BBOV_III010870(17.m07935)
CPV: cgd3_320
SMIN: v1.2.001977.t1(symbB.v1.2.001977.t1) v1.2.001979.t1(symbB.v1.2.001979.t1) v1.2.039797.t1(symbB.v1.2.039797.t1) v1.2.039797.t2(symbB.v1.2.039797.t2)
TPS: THAPSDRAFT_3304(MNT1)
LMA: LMJF_32_0080(NMT)
LIF: LINJ_32_0080(NMT)
 » show all
Taxonomy
Reference
1  [PMID:3566958]
  Authors
Guertin D, Grise-Miron L, Riendeau D.
  Title
Identification of a 51-kilodalton polypeptide fatty acyl chain acceptor in soluble extracts from mouse cardiac tissue.
  Journal
Biochem Cell Biol 64:1249-55 (1986)
Reference
2  [PMID:3123489]
  Authors
Heuckeroth RO, Towler DA, Adams SP, Glaser L, Gordon JI.
  Title
11-(Ethylthio)undecanoic acid. A myristic acid analogue of altered hydrophobicity which is functional for peptide N-myristoylation with wheat germ and yeast acyltransferase.
  Journal
J Biol Chem 263:2127-33 (1988)
Reference
3  [PMID:3106975]
  Authors
Towler DA, Adams SP, Eubanks SR, Towery DS, Jackson-Machelski E, Glaser L, Gordon JI
  Title
Purification and characterization of yeast myristoyl CoA:protein N-myristoyltransferase.
  Journal
Proc Natl Acad Sci U S A 84:2708-12 (1987)
DOI:10.1073/pnas.84.9.2708
Reference
4  [PMID:9677304]
  Authors
McIlhinney RA, Young K, Egerton M, Camble R, White A, Soloviev M
  Title
Characterization of human and rat brain myristoyl-CoA:protein N-myristoyltransferase: evidence for an alternative splice variant of the enzyme.
  Journal
Biochem J 333 ( Pt 3):491-5 (1998)
Reference
5  [PMID:11371195]
  Authors
Farazi TA, Waksman G, Gordon JI
  Title
Structures of Saccharomyces cerevisiae N-myristoyltransferase with bound myristoylCoA and peptide provide insights about substrate recognition and catalysis.
  Journal
Biochemistry 40:6335-43 (2001)
DOI:10.1021/bi0101401
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.97
IUBMB Enzyme Nomenclature: 2.3.1.97
ExPASy - ENZYME nomenclature database: 2.3.1.97
BRENDA, the Enzyme Database: 2.3.1.97
CAS: 110071-61-9

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