KEGG   ENZYME: 2.3.2.17Help
Entry
EC 2.3.2.17                 Enzyme                                 

Name
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase;
femA (gene name);
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
BRITE hierarchy
Sysname
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc:glycine glycyltransferase
Reaction(IUBMB)
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNAGly [RN:R08777]
Reaction(KEGG)
Substrate
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
glycyl-tRNA(Gly) [CPD:C02412]
Product
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
tRNA(Gly) [CPD:C01642]
Comment
This enzyme catalyses the successive transfer of two Gly moieties from charged tRNAs to MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc, attaching them to a Gly residue previously attached by EC 2.3.2.16 (lipid II:glycine glycyltransferase) to the N6 of the L-Lys at position 3 of the pentapeptide. This is the second step in the synthesis of the pentaglycine interpeptide bridge that is used by Staphylococcus aureus for the crosslinking of different glycan strands to each other. The next step is catalysed by EC 2.3.2.18 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase). This enzyme is essential for methicillin resistance [1].
History
EC 2.3.2.17 created 2010
Pathway
ec00550  Peptidoglycan biosynthesis
ec01100  Metabolic pathways
Orthology
K11694  peptidoglycan pentaglycine glycine transferase (the second and third glycine)
Genes
SAU: SA1206(femA)
SAV: SAV1374(femA)
SAW: SAHV_1362(femA)
SAH: SaurJH1_1464
SAJ: SaurJH9_1435
SAM: MW1261(femA)
SAS: SAS1314
SAR: SAR1387(femA)
SAC: SACOL1410(femA)
SAX: USA300HOU_1309(femA)
SAA: SAUSA300_1269(femA)
SAE: NWMN_1286(femA)
SAD: SAAV_1356(femA)
SUE: SAOV_1384
SUJ: SAA6159_01241(femA)
SUK: SAA6008_01339(femA)
SUZ: MS7_1331
SUX: SAEMRSA15_12220(femA)
SUW: SATW20_13740(femA)
SUG: SAPIG1375
SUF: SARLGA251_12830(femA)
SAUA: SAAG_01984
SAUE: RSAU_001255(femA)
SAUS: SA40_1251(femA)
SAUU: SA957_1266(femA)
SAUG: SA268_1271(femA)
SAUZ: SAZ172_1385(femA)
SAUF: X998_1382(femA)
SAB: SAB1229(femA)
SUY: SA2981_1328(femA)
SAUB: C248_1412(femA)
SAUC: CA347_1311(femA)
SAUR: SABB_00138(femA)
SAUI: AZ30_06695
SAUD: CH52_12195
SAMS: NI36_06780
SEP: SE1057
SER: SERP0946(femA)
SEPP: SEB_01083
SEPS: DP17_11(femA)
SHA: SH1532(femA)
SHH: ShL2_01411(femA)
SSP: SSP1371
SCA: SCA_1019(femA)
SLN: SLUG_14700(femA)
SDT: SPSE_1403(femA)
SPAS: STP1_2404(femA2)
SXO: SXYL_01495(femA)
SHU: SHYC_07545(femA)
SCAP: AYP1020_0683(femA_1)
SSCH: LH95_06785
SSCZ: RN70_07255
SAGQ: EP23_02880
MCL: MCCL_1041(femA)
MCAK: MCCS_12180(femA_2)
SIK: K710_1043
TPYO: X956_00015
 » show all
Taxonomy
Reference
1  [PMID:2559314]
  Authors
Berger-Bachi B, Barberis-Maino L, Strassle A, Kayser FH
  Title
FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: molecular cloning and characterization.
  Journal
Mol Gen Genet 219:263-9 (1989)
  Sequence
[sau:SA1206]
Reference
2  [PMID:7590176]
  Authors
Johnson S, Kruger D, Labischinski H
  Title
FemA of Staphylococcus aureus: isolation and immunodetection.
  Journal
FEMS Microbiol Lett 132:221-8 (1995)
DOI:10.1111/j.1574-6968.1995.tb07837.x
  Sequence
Reference
3  [PMID:12176388]
  Authors
Benson TE, Prince DB, Mutchler VT, Curry KA, Ho AM, Sarver RW, Hagadorn JC, Choi GH, Garlick RL
  Title
X-ray crystal structure of Staphylococcus aureus FemA.
  Journal
Structure 10:1107-15 (2002)
DOI:10.1016/S0969-2126(02)00807-9
  Sequence
Reference
4  [PMID:15228543]
  Authors
Schneider T, Senn MM, Berger-Bachi B, Tossi A, Sahl HG, Wiedemann I
  Title
In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus.
  Journal
Mol Microbiol 53:675-85 (2004)
DOI:10.1111/j.1365-2958.2004.04149.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.17
IUBMB Enzyme Nomenclature: 2.3.2.17
ExPASy - ENZYME nomenclature database: 2.3.2.17
BRENDA, the Enzyme Database: 2.3.2.17

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