KEGG   ENZYME: 2.3.2.18Help
Entry
EC 2.3.2.18                 Enzyme                                 

Name
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase;
femB (gene name);
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-triglycine)-D-alanyl-D-alanine-ditrans,octacis-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
BRITE hierarchy
Sysname
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc:glycine glycyltransferase
Reaction(IUBMB)
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 glycyl-tRNAGly = MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-penta-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc + 2 tRNAGly [RN:R08778]
Reaction(KEGG)
Substrate
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
glycyl-tRNA(Gly) [CPD:C02412]
Product
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-penta-Gly)-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc;
tRNA(Gly) [CPD:C01642]
Comment
This Staphylococcus aureus enzyme catalyses the successive transfer of two Gly moieties from charged tRNAs to MurNAc-L-Ala-D-isoglutaminyl-L-Lys-(N6-tri-Gly)-D-Ala-D-Ala-diphosphoundecaprenyl-GlcNAc, attaching them to the three Gly molecules that were previously attached to the N6 of the L-Lys at position 3 of the pentapeptide by EC 2.3.2.16 (lipid II:glycine glycyltransferase) and EC 2.3.2.17 (N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-(N6-glycyl)-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine glycyltransferase). This is the last step in the synthesis of the pentaglycine interpeptide bridge that is used in this organism for the crosslinking of different glycan strands to each other.
History
EC 2.3.2.18 created 2010
Pathway
ec00550  Peptidoglycan biosynthesis
ec01100  Metabolic pathways
Orthology
K11695  peptidoglycan pentaglycine glycine transferase (the fourth and fifth glycine)
Genes
SAU: SA1207(femB)
SAV: SAV1375(femB)
SAW: SAHV_1363(femB)
SAH: SaurJH1_1465
SAJ: SaurJH9_1436
SAM: MW1262(femB)
SAS: SAS1315
SAR: SAR1388(femB)
SAC: SACOL1411
SAX: USA300HOU_1310(femB)
SAA: SAUSA300_1270(femB)
SAE: NWMN_1287(femB)
SAD: SAAV_1357
SUE: SAOV_1385
SUJ: SAA6159_01242(femB)
SUK: SAA6008_01340(femB)
SUQ: HMPREF0772_11832(femB)
SUZ: MS7_1332(femB)
SUX: SAEMRSA15_12230(femB)
SUW: SATW20_13750(femB)
SUG: SAPIG1376
SUF: SARLGA251_12840(femB)
SAUA: SAAG_01985
SAUE: RSAU_001256(femB)
SAUS: SA40_1252(femB)
SAUU: SA957_1267(femB)
SAUG: SA268_1272(femB)
SAUZ: SAZ172_1386(femB)
SAUT: SAI1T1_2009900(femB)
SAUJ: SAI2T2_1009920(femB)
SAUK: SAI3T3_1009910(femB)
SAUQ: SAI4T8_1009900(femB)
SAUV: SAI7S6_1009910(femB)
SAUW: SAI5S5_1009870(femB)
SAUX: SAI6T6_1009880(femB)
SAUY: SAI8T7_1009910(femB)
SAUF: X998_1383(femB)
SAB: SAB1230(femB)
SUY: SA2981_1329(femB)
SAUB: C248_1413(femB)
SAUC: CA347_1312(femB)
SAUR: SABB_00137(femB)
SAUI: AZ30_06700
SAUD: CH52_12190
SAMS: NI36_06785
SEP: SE1058
SER: SERP0947(femB)
SEPP: SEB_01084
SEPS: DP17_12(femB)
SHA: SH1531(femB)
SHH: ShL2_01410(femB)
SSP: SSP1370
SCA: SCA_1020(femB)
SLN: SLUG_14710(femB)
SDT: SPSE_1402(femB)
SPAS: STP1_2405(femB)
SXO: SXYL_01494(femB)
SHU: SHYC_07540(femB-2)
SCAP: AYP1020_0684(femB)
SSCH: LH95_06780
SSCZ: RN70_07250
SAGQ: EP23_02885
MCL: MCCL_1042(femB)
MCAK: MCCS_12190(femB)
GMO: NCTC11323_00360(femB_2)
 » show all
Taxonomy
Reference
1  [PMID:9393725]
  Authors
Ehlert K, Schroder W, Labischinski H
  Title
Specificities of FemA and FemB for different glycine residues: FemB cannot substitute for FemA in staphylococcal peptidoglycan pentaglycine side chain formation.
  Journal
J Bacteriol 179:7573-6 (1997)
DOI:10.1128/JB.179.23.7573-7576.1997
  Sequence
Reference
2  [PMID:14523106]
  Authors
Rohrer S, Berger-Bachi B
  Title
Application of a bacterial two-hybrid system for the analysis of protein-protein  interactions between FemABX family proteins.
  Journal
Microbiology 149:2733-8 (2003)
DOI:10.1099/mic.0.26315-0
  Sequence
Reference
3  [PMID:15228543]
  Authors
Schneider T, Senn MM, Berger-Bachi B, Tossi A, Sahl HG, Wiedemann I
  Title
In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus.
  Journal
Mol Microbiol 53:675-85 (2004)
DOI:10.1111/j.1365-2958.2004.04149.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.18
IUBMB Enzyme Nomenclature: 2.3.2.18
ExPASy - ENZYME nomenclature database: 2.3.2.18
BRENDA, the Enzyme Database: 2.3.2.18

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