KEGG   ENZYME: 2.3.2.24Help
Entry
EC 2.3.2.24                 Enzyme                                 

Name
(E3-independent) E2 ubiquitin-conjugating enzyme;
E2-230K;
UBE2O;
E3-independent ubiquitin-conjugating enzyme E2
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
BRITE hierarchy
Sysname
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine:L-lysine ubiquitinyl transferase ([E3 ubiquitin transferase]-independent)
Reaction(IUBMB)
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine (overall reaction);
(1a) [E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) ubiquitin-conjugating enzyme]-S-monoubiquitinyl-L-cysteine;
(1b) [(E3-independent) E2 ubiquitin-conjugating E2 enzyme]-S-monoubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine
Substrate
[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine;
[acceptor protein]-L-lysine;
[(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine;
[(E3-independent) E2 ubiquitin-conjugating E2 enzyme]-S-monoubiquitinyl-L-cysteine
Product
[E1 ubiquitin-activating enzyme]-L-cysteine;
[acceptor protein]-N6-monoubiquitinyl-L-lysine;
[(E3-independent) ubiquitin-conjugating enzyme]-S-monoubiquitinyl-L-cysteine;
[(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine
Comment
The enzyme transfers a single ubiquitin directly from an ubiquitinated E1 ubiquitin-activating enzyme to itself, and on to a lysine residue of the acceptor protein without involvement of E3 ubiquitin transferases (cf. EC 2.3.2.26, EC 2.3.2.27). It forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and Mg2+-ATP and catalyses the conjugation of ubiquitin to protein substrates, independently of E3. This transfer has only been observed with small proteins. In vitro a transfer to small acceptors (e.g. L-lysine, N-acetyl-L-lysine methyl ester) has been observed [1].
History
EC 2.3.2.24 created 2015
Orthology
K10581  ubiquitin-conjugating enzyme E2 O
Genes
HSA: 63893(UBE2O)
PTR: 468335(UBE2O)
PPS: 100976425(UBE2O)
GGO: 101127491(UBE2O)
PON: 100433599(UBE2O)
NLE: 100599786(UBE2O)
MCC: 710529(UBE2O)
MCF: 102127058(UBE2O)
CSAB: 103242990(UBE2O)
RRO: 104681273(UBE2O)
RBB: 108537423(UBE2O)
CJC: 100394130(UBE2O)
SBQ: 101028721(UBE2O)
MMU: 217342(Ube2o)
RNO: 303689(Ube2o)
CGE: 100762350(Ube2o)
HGL: 101713045(Ube2o)
CCAN: 109685604
OCU: 100355504(UBE2O)
TUP: 102470674(UBE2O)
CFA: 483330(UBE2O)
AML: 100482599(UBE2O)
UMR: 103665762(UBE2O)
ORO: 101375521(UBE2O)
FCA: 101097933(UBE2O)
PTG: 102966677(UBE2O)
AJU: 106987763(UBE2O)
BTA: 789422(UBE2O)
BOM: 102285394(UBE2O)
BIU: 109573989(UBE2O)
PHD: 102327806(UBE2O)
CHX: 102191553(UBE2O)
OAS: 101116847(UBE2O)
SSC: 100519047(UBE2O)
CFR: 102522952(UBE2O)
CDK: 105092186(UBE2O)
BACU: 103011378(UBE2O)
LVE: 103070455(UBE2O)
OOR: 101279654(UBE2O)
ECB: 100058843(UBE2O)
EPZ: 103541303(UBE2O)
EAI: 106830205(UBE2O)
MYB: 102258004(UBE2O)
MYD: 102762782(UBE2O)
HAI: 109383760(UBE2O)
RSS: 109439986 109454674(UBE2O)
PALE: 102877740(UBE2O)
LAV: 104846542(UBE2O)
TMU: 101354325
MDO: 100619214(UBE2O)
SHR: 100914729(UBE2O)
OAA: 100076608(UBE2O)
GGA: 417359(UBE2O)
MGP: 100539166(UBE2O)
CJO: 107322118(UBE2O)
APLA: 101798552(UBE2O)
ACYG: 106034705(UBE2O)
TGU: 100225560(UBE2O)
GFR: 102044693(UBE2O)
FAB: 101817887(UBE2O)
PHI: 102106940(UBE2O)
PMAJ: 107212568(UBE2O)
CCAE: 111937274(UBE2O)
CCW: 104694393(UBE2O)
FPG: 101916974(UBE2O)
FCH: 102045675(UBE2O)
CLV: 102093303(UBE2O)
EGZ: 104125380(UBE2O)
AAM: 106485395(UBE2O)
ASN: 102379656(UBE2O)
AMJ: 102568632(UBE2O)
PSS: 102464036(UBE2O)
CMY: 102935279(UBE2O)
CPIC: 101950220(UBE2O)
ACS: 100552973(ube2o)
PVT: 110085899(UBE2O)
PBI: 103060302(UBE2O)
GJA: 107121145(UBE2O)
XTR: 100327234(ube2o)
NPR: 108796859(UBE2O)
DRE: 100331775(ube2o)
SRX: 107715484(ube2o)
SANH: 107702992(ube2o)
SGH: 107573494(ube2o)
CCAR: 109068918(ube2o)
IPU: 108273099(ube2o)
AMEX: 103022781(ube2o)
LCO: 104932981(ube2o)
MZE: 101472861(ube2o)
OLA: 101167846(ube2o)
XMA: 102228272(ube2o)
PRET: 103469664(ube2o)
NFU: 107382242(ube2o)
KMR: 108248778(ube2o)
CSEM: 103383774(ube2o)
LCF: 108875850(ube2o)
SDU: 111236315(ube2o)
HCQ: 109519879(ube2o)
BPEC: 110166226(ube2o)
MALB: 109966274(ube2o)
ELS: 105021628(ube2o)
SFM: 108939871(ube2o)
LCM: 102349983(UBE2O)
CMK: 103191118(ube2o)
SPU: 586721
APLC: 110980920
SKO: 102802790
DME: Dmel_CG10254(CG10254)
DSI: Dsimw501_GD18366(Dsim_GD18366)
MDE: 101898374
AAG: 5567422
AME: 724799
BIM: 100748178
BTER: 100645522
SOC: 105198011
AEC: 105145030
ACEP: 105619366
PBAR: 105430228
HST: 105189881
DQU: 106742004
CFO: 105249030
LHU: 105669127
PGC: 109858322
PCF: 106791680
NVI: 100118905
MDL: 103570750
TCA: 659924
DPA: 109546527
NVL: 108566284
BMOR: 101740080
PRAP: 111000908
HAW: 110377903
DNX: 107166601
CLEC: 106673859
ZNE: 110839013
CRG: 105342002
MYI: 110465099
OBI: 106868036
LAK: 106174980
SHX: MS3_00798
EPA: 110240082
ADF: 107356386
HMG: 100203835
ATH: AT1G53023 AT1G53025 AT2G16920(UBC23) AT2G33770(PHO2) AT3G15355(UBC25)
LJA: Lj0g3v0123939.1(Lj0g3v0123939.1) Lj0g3v0266399.1(Lj0g3v0266399.1) Lj0g3v0304639.1(Lj0g3v0304639.1) Lj0g3v0326929.1(Lj0g3v0326929.1) Lj0g3v0355129.1(Lj0g3v0355129.1) Lj1g3v1651360.1(Lj1g3v1651360.1) Lj2g3v3054240.1(Lj2g3v3054240.1) Lj3g3v1666210.1(Lj3g3v1666210.1) Lj4g3v0451220.1(Lj4g3v0451220.1) Lj4g3v0451220.2(Lj4g3v0451220.2) Lj4g3v2435000.1(Lj4g3v2435000.1) Lj4g3v2435000.2(Lj4g3v2435000.2) Lj5g3v2244630.1(Lj5g3v2244630.1) Lj6g3v2030860.1(Lj6g3v2030860.1)
DOSA: Os01t0125900-01(Os01g0125900) Os01t0232500-01(Os01g0232500) Os01t0233900-01(Os01g0233900) Os01t0677500-00(Os01g0677500) Os05t0153132-00(Os05g0153132) Os05t0232500-01(Os05g0232500) Os05t0557600-01(Os05g0557600) Os09t0294300-01(Os09g0294300)
ATS: 109733100(LOC109733100) 109739038(LOC109739038) 109739120(LOC109739120) 109746297(LOC109746297) 109748786(LOC109748786) 109749680(LOC109749680) 109751578(LOC109751578) 109751580(LOC109751580) 109753429(LOC109753429) 109757315(LOC109757315) 109760591(LOC109760591) 109760592(LOC109760592) 109767283(LOC109767283) 109768423(LOC109768423) 109773380(LOC109773380) 109776213(LOC109776213) 109776215(LOC109776215) 109776715(LOC109776715) 109780160(LOC109780160) 109786599(LOC109786599) 109787010(LOC109787010)
ANI: AN3136.2
ANG: ANI_1_2850024(An02g08940)
PNO: SNOG_05701(SNOG_05700)
PTE: PTT_18749
CNE: CNF01270
CNB: CNBF3440
HIR: HETIRDRAFT_166454(ubc3)
MRR: Moror_480
ABP: AGABI1DRAFT117056(AGABI1DRAFT_117056)
ABV: AGABI2DRAFT114397(AGABI2DRAFT_114397)
DFA: DFA_11688
SMIN: v1.2.012600.t1(symbB.v1.2.012600.t1)
 » show all
Taxonomy
Reference
1  [PMID:2981864]
  Authors
Pickart CM, Rose IA
  Title
Functional heterogeneity of ubiquitin carrier proteins.
  Journal
J Biol Chem 260:1573-81 (1985)
Reference
2  [PMID:17588522]
  Authors
Hoeller D, Hecker CM, Wagner S, Rogov V, Dotsch V, Dikic I
  Title
E3-independent monoubiquitination of ubiquitin-binding proteins.
  Journal
Mol Cell 26:891-8 (2007)
DOI:10.1016/j.molcel.2007.05.014
Reference
3  [PMID:23701900]
  Authors
Ramanathan HN, Zhang G, Ye Y
  Title
Monoubiquitination of EEA1 regulates endosome fusion and trafficking.
  Journal
Cell Biosci 3:24 (2013)
DOI:10.1186/2045-3701-3-24
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.24
IUBMB Enzyme Nomenclature: 2.3.2.24
ExPASy - ENZYME nomenclature database: 2.3.2.24
BRENDA, the Enzyme Database: 2.3.2.24

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