KEGG   ENZYME: 2.3.2.25Help
Entry
EC 2.3.2.25                 Enzyme                                 

Name
N-terminal E2 ubiquitin-conjugating enzyme;
Ube2w;
N-terminal ubiquitin-conjugating enzyme E2
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
BRITE hierarchy
Sysname
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine:acceptor protein ubiquitin ligase (peptide bond-forming)
Reaction(IUBMB)
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [E1 ubiquitin-activating enzyme]-L-cysteine + N-terminal-ubiquitinyl-[acceptor protein] (overall reaction);
(1a) S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[N-terminal ubiquitin-conjugating enzyme]-L-cysteine;
(1b) S-ubiquitinyl-[N-terminal E2 ubiquitin-conjugating E2 enzyme]-L-cysteine + [acceptor protein]-N-terminal-amino acid = [N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine + N-ubiquitinyl-[acceptor protein]-N-terminal amino acid
Substrate
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine;
[acceptor protein]-N-terminal-amino acid;
[N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine;
S-ubiquitinyl-[N-terminal E2 ubiquitin-conjugating E2 enzyme]-L-cysteine
Product
[E1 ubiquitin-activating enzyme]-L-cysteine;
N-terminal-ubiquitinyl-[acceptor protein];
S-ubiquitinyl-[N-terminal ubiquitin-conjugating enzyme]-L-cysteine;
[N-terminal E2 ubiquitin-conjugating enzyme]-L-cysteine;
N-ubiquitinyl-[acceptor protein]-N-terminal amino acid
Comment
The enzyme ubiquitinylates the N-terminus of the acceptor protein. It is not reactive towards free lysine.
History
EC 2.3.2.25 created 2015
Orthology
K10688  ubiquitin-conjugating enzyme E2 W
Genes
HSA: 55284(UBE2W)
PTR: 464234(UBE2W)
PPS: 100968686(UBE2W)
GGO: 101125477(UBE2W)
PON: 100436654(UBE2W)
NLE: 100595750(UBE2W)
MCC: 696276(UBE2W)
MCF: 102114921(UBE2W)
CSAB: 103236976(UBE2W)
RRO: 104664483(UBE2W)
RBB: 108515041(UBE2W)
CJC: 100411128(UBE2W)
SBQ: 101049437(UBE2W)
MMU: 66799(Ube2w)
RNO: 100909445(Ube2w) 103694506
CGE: 100762319(Ube2w)
NGI: 103746622(Ube2w)
HGL: 101724035(Ube2w)
CCAN: 109692113(Ube2w)
OCU: 100340823(UBE2W) 103347952
TUP: 102471306(UBE2W)
CFA: 486999(UBE2W)
AML: 100470712(UBE2W)
UMR: 103681261(UBE2W)
ORO: 101366102(UBE2W)
FCA: 101100361(UBE2W)
PTG: 102963747(UBE2W)
AJU: 106968387(UBE2W)
BTA: 613423(UBE2W)
BOM: 102267353(UBE2W)
PHD: 102331416
CHX: 102182239(UBE2W)
OAS: 101107531(UBE2W)
SSC: 100152196(UBE2W)
CFR: 102522747(UBE2W)
CDK: 105088551(UBE2W)
BACU: 103018032(UBE2W)
LVE: 103070624(UBE2W)
OOR: 101269254(UBE2W)
ECB: 100051082(UBE2W)
EPZ: 103565253(UBE2W)
EAI: 106839238(UBE2W)
MYB: 102254981(UBE2W)
MYD: 102774697(UBE2W)
HAI: 109383300(UBE2W)
RSS: 109444460(UBE2W)
PALE: 102895199(UBE2W)
LAV: 100655720(UBE2W)
TMU: 101359004
MDO: 100028124(UBE2W)
SHR: 100930487(UBE2W)
OAA: 100089545(UBE2W)
GGA: 420185(UBE2W)
MGP: 100547672(UBE2W)
CJO: 107310283(UBE2W)
APLA: 101792772(UBE2W)
ACYG: 106030591(UBE2W)
TGU: 100227910(UBE2W)
SCAN: 103812906(UBE2W)
GFR: 102033188(UBE2W)
FAB: 101813609(UBE2W)
PHI: 102109159(UBE2W)
PMAJ: 107199696(UBE2W)
CCAE: 111924643(UBE2W)
CCW: 104692817(UBE2W)
FPG: 101913697(UBE2W)
FCH: 102051497(UBE2W)
CLV: 102090304(UBE2W)
EGZ: 104122379(UBE2W)
NNI: 104016171(UBE2W)
ACUN: 113476554(UBE2W)
AAM: 106484622(UBE2W)
ASN: 102384644(UBE2W)
AMJ: 102574930(UBE2W)
PSS: 102443519(UBE2W)
CMY: 102941571(UBE2W)
CPIC: 101950923(UBE2W)
ACS: 100561037(ube2w)
PVT: 110089643(UBE2W)
PBI: 103048188(UBE2W)
PMUR: 107297275(UBE2W)
GJA: 107114639(UBE2W)
XLA: 108695347(ube2w.S) 108705626
XTR: 548886(ube2w)
NPR: 108797560(UBE2W)
DRE: 553013(ube2w) 692325(flj11011l)
PHYP: 113541067(ube2w) 113543281
TRU: 101065623(ube2w)
LCO: 104921310(ube2w)
NCC: 104967921(ube2w)
MZE: 101487815(ube2w)
OLA: 101168999(ube2w)
XMA: 102224996(ube2w)
PRET: 103456677(ube2w)
NFU: 107382925(ube2w)
KMR: 108230264(ube2w)
CSEM: 103377002(ube2w)
LCF: 108881822(ube2w)
SDU: 111221031(ube2w)
HCQ: 109517000(ube2w)
BPEC: 110160941(ube2w)
MALB: 109960119(ube2w)
SASA: 106579737(ube2w) 106591739
OTW: 112236392(ube2w)
SALP: 112072669(ube2w)
ELS: 105019654(ube2w)
SFM: 108935406(ube2w)
LCM: 102361547(UBE2W)
CMK: 103174572(ube2w)
CIN: 100177184
SPU: 582071
APLC: 110988165
SKO: 100371975
DME: Dmel_CG7220(CG7220)
DER: 6547556
DPE: 6592511
DSI: Dsimw501_GD10753(Dsim_GD10753)
DWI: 6639848
MDE: 101889802
AAG: 5570741
AME: 409100
BIM: 100743714
BTER: 100648710
SOC: 105193427
MPHA: 105834299
AEC: 105145047
ACEP: 105619446
PBAR: 105430277
HST: 105189835
DQU: 106749409
CFO: 105255644
LHU: 105678064
PGC: 109856413
OBO: 105279036
PCF: 106791725
MDL: 103573819
TCA: 663661
DPA: 109544758
NVL: 108563601
BMOR: 101742775
PMAC: 106709150
PRAP: 111003478
TNL: 113498056
CLEC: 106663108
ZNE: 110831634
FCD: 110842473
TUT: 107366439
DPTE: 113799677
CEL: CELE_Y54E5B.4(ubc-16)
CBR: CBG19703(Cbr-ubc-16)
BMY: Bm1_47885
TSP: Tsp_07604
CRG: 105319798
MYI: 110457543
OBI: 106871524
LAK: 106169942
EPA: 110241364
ADF: 107341212
PDAM: 113670640
SPIS: 111332059
HMG: 100208384
AQU: 100635218
ATH: AT1G45050(ATUBC2-1) AT1G75440(UBC16) AT4G36410(UBC17) AT5G42990(UBC18)
EGR: 104419696
VRA: 106776419
VAR: 108335984
CCAJ: 109799881
LJA: Lj0g3v0299709.1(Lj0g3v0299709.1)
ADU: 107488018
AIP: 107642925
CSV: 101202936
CMO: 103493218
MCHA: 111008493
DOSA: Os03t0681400-01(Os03g0681400) Os12t0636800-01(Os12g0636800)
ATS: 109759242(LOC109759242) 109762224(LOC109762224)
ZMA: 100272309 100280549 100282569(pco120990)
ATR: 18429279
APRO: F751_3522
CAL: CAALFM_C210580WA(UBC15)
NCR: NCU09122
NTE: NEUTE1DRAFT90783(NEUTE1DRAFT_90783)
MGR: MGG_09913
SSCK: SPSK_09847
MAW: MAC_00992
MAJ: MAA_05741
CMT: CCM_02340
MBE: MBM_02438
ANI: AN2413.2
ANG: ANI_1_760024(An02g05480)
ABE: ARB_04411
TVE: TRV_06810
PTE: PTT_07968
CNE: CNE04250
CNB: CNBE4240
HIR: HETIRDRAFT_145590(ubc2)
MRR: Moror_347
ABP: AGABI1DRAFT81426(AGABI1DRAFT_81426)
ABV: AGABI2DRAFT189796(AGABI2DRAFT_189796)
DDI: DDB_G0268938(ube2w)
DFA: DFA_07886(ube2w)
PYO: PY17X_1032100(PY05645)
PCB: PCHAS_103050(PC302472.00.0)
TAN: TA14305
TPV: TP02_0640
BBO: BBOV_II006740(18.m06553)
SMIN: v1.2.018903.t1(symbB.v1.2.018903.t1)
 » show all
Taxonomy
Reference
1  [PMID:9774340]
  Authors
Breitschopf K, Bengal E, Ziv T, Admon A, Ciechanover A
  Title
A novel site for ubiquitination: the N-terminal residue, and not internal lysines of MyoD, is essential for conjugation and degradation of the protein.
  Journal
EMBO J 17:5964-73 (1998)
DOI:10.1093/emboj/17.20.5964
Reference
2  [PMID:23560854]
  Authors
Tatham MH, Plechanovova A, Jaffray EG, Salmen H, Hay RT
  Title
Ube2W conjugates ubiquitin to alpha-amino groups of protein N-termini.
  Journal
Biochem J 453:137-45 (2013)
DOI:10.1042/BJ20130244
  Sequence
[hsa:55284]
Reference
3  [PMID:23696636]
  Authors
Scaglione KM, Basrur V, Ashraf NS, Konen JR, Elenitoba-Johnson KS, Todi SV, Paulson HL
  Title
The ubiquitin-conjugating enzyme (E2) Ube2w ubiquitinates the N terminus of substrates.
  Journal
J Biol Chem 288:18784-8 (2013)
DOI:10.1074/jbc.C113.477596
  Sequence
[hsa:55284]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.25
IUBMB Enzyme Nomenclature: 2.3.2.25
ExPASy - ENZYME nomenclature database: 2.3.2.25
BRENDA, the Enzyme Database: 2.3.2.25

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