KEGG   ENZYME: 2.4.1.14Help
Entry
EC 2.4.1.14                 Enzyme                                 

Name
sucrose-phosphate synthase;
UDP-glucose---fructose-phosphate glucosyltransferase;
sucrosephosphate---UDP glucosyltransferase;
UDP-glucose-fructose-phosphate glucosyltransferase;
SPS;
uridine diphosphoglucose-fructose phosphate glucosyltransferase;
sucrose 6-phosphate synthase;
sucrose phosphate synthetase;
sucrose phosphate-uridine diphosphate glucosyltransferase;
sucrose phosphate synthase;
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase (configuration-retaining)
Reaction(IUBMB)
UDP-alpha-D-glucose + D-fructose 6-phosphate = UDP + sucrose 6F-phosphate [RN:R00766 R06073]
Reaction(KEGG)
Substrate
UDP-alpha-D-glucose [CPD:C00029];
D-fructose 6-phosphate [CPD:C00085]
Product
UDP [CPD:C00015];
sucrose 6F-phosphate [CPD:C02591]
Comment
Requires Mg2+ or Mn2+ for maximal activity [2]. The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates [2]. The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not [2]. While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible [3]. The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.
History
EC 2.4.1.14 created 1961, modified 2008
Pathway
ec00500  Starch and sucrose metabolism
ec01100  Metabolic pathways
Orthology
K00696  sucrose-phosphate synthase
Genes
RSS: 109441750
ATH: AT1G04920(SPS3F) AT4G10120(ATSPS4F) AT5G11110(SPS2F) AT5G20280(SPS1F)
ALY: ARALYDRAFT_470477 ARALYDRAFT_487943 ARALYDRAFT_488935 ARALYDRAFT_489859
CRB: 111829932 17881554 17884088 17900430
CSAT: 104705376 104706379 104707307 104721265 104736104 104737013 104737937 104739022 104754635 104762737 104769326 104770411
EUS: EUTSA_v10006639mg EUTSA_v10012540mg EUTSA_v10012643mg EUTSA_v10028384mg
BRP: 103844199 103845691 103851339 103853568 103855902 103874391
BNA: 106348525 106366768 106370972 106371763 106377423 106397279 106399433 106407297 106410155 106425833 106430056 106439492 111210345
BOE: 106307062 106318106 106327013 106327645 106328688 106343737
THJ: 104799636 104801022 104824613
LJA: Lj0g3v0058449.1(Lj0g3v0058449.1) Lj0g3v0281579.1(Lj0g3v0281579.1) Lj2g3v3101760.1(Lj2g3v3101760.1) Lj3g3v1955590.1(Lj3g3v1955590.1) Lj4g3v1893200.1(Lj4g3v1893200.1)
BVG: 104901950(SBSPS1) 104909043
DOSA: Os01t0919400-00(Os01g0919400) Os02t0184400-01(Os02g0184400) Os06t0643800-01(Os06g0643800) Os08t0301500-01(Os08g0301500) Os11t0236100-01(Os11g0236100)
ATS: 109738481(LOC109738481) 109762784(LOC109762784) 109780026(LOC109780026) 109780714(LOC109780714) 109787086(LOC109787086)
CRE: CHLREDRAFT_105328(GTR7)
APRO: F751_1987
MAH: MEALZ_0614(sps)
TCX: Tcr_1794
MEJ: Q7A_1564
MEC: Q7C_247
NOC: Noc_3069
NHL: Nhal_3941
NWA: Nwat_3124
TEE: Tel_08340
TGR: Tgr7_0708
TKM: TK90_2312
TNI: TVNIR_0279(mfpsA_[H])
TVR: TVD_01115
GAI: IMCC3135_06955(mfpsA_2)
NEU: NE1213(sps)
NET: Neut_1080
MFA: Mfla_2610
MEP: MPQ_2706
DEU: DBW_2297(mfpsA)
DBA: Dbac_2912
DSF: UWK_00548
DOL: Dole_0650
DAT: HRM2_24290(spsA)
MEX: Mext_0200
MDI: METDI0081
MCH: Mchl_0143
MPO: Mpop_0284
MOR: MOC_0555
META: Y590_00410
MAQU: Maq22A_1p31500(rfaG)
ANH: A6F65_02402(mfpsA)
MGM: Mmc1_3516
AFR: AFE_1551
ACU: Atc_1728
JDA: BW727_101969(mshA)
MBJ: KQ51_01499(mfpsA)
SYN: sll0045(sps)
SYZ: MYO_128450(sps)
SYY: SYNGTS_2819(sps)
SYT: SYNGTI_2818(sps)
SYS: SYNPCCN_2817(sps)
SYQ: SYNPCCP_2817(sps)
SYJ: D082_07330(spsA)
SYW: SYNW2520(sps)
SYC: syc0730_d(sps)
SYG: sync_2936
SYR: SynRCC307_2529(sps)
SYP: SYNPCC7002_A0888(spsA)
SYNR: KR49_08880
SYND: KR52_02500
SYH: Syncc8109_2757(spsA)
SYNW: SynWH8103_02908(sps_sds)
TEL: tlr0582(sps)
THN: NK55_11890(sps)
LET: O77CONTIG1_00929(mfpsA_1)
PMA: Pro_1880(rfaG)
PMM: PMM1711(sps)
PMT: PMT_2268
PMF: P9303_30171(sps)
PME: NATL1_21951(sps)
PRC: EW14_2080
PRM: EW15_2282
RBA: RB5197(sps)
PIR: VN12_23145(mfpsA_3)
PLM: Plim_3683
FMR: Fuma_03286(mfpsA_2)
LBY: Lbys_0264
FJO: Fjoh_3967
FJG: BB050_03561(mfpsA)
MRO: MROS_1315
TME: Tmel_0533
TAF: THA_1341
THER: Y592_06205
FNO: Fnod_0446
PMO: Pmob_1867
DTN: DTL3_0896
KOL: Kole_1069
 » show all
Taxonomy
Reference
1  [PMID:13769376]
  Authors
MENDICINO J.
  Title
Sucrose phosphate synthesis in wheat germ and green leaves.
  Journal
J Biol Chem 235:3347-52 (1960)
Reference
2  [PMID:9852031]
  Authors
Curatti L, Folco E, Desplats P, Abratti G, Limones V, Herrera-Estrella L, Salerno G
  Title
Sucrose-phosphate synthase from Synechocystis sp. strain PCC 6803: identification of the spsA gene and characterization of the enzyme expressed in Escherichia coli.
  Journal
J Bacteriol 180:6776-9 (1998)
Reference
3  [PMID:15012296]
  Authors
Huber SC, Huber JL
  Title
ROLE AND REGULATION OF SUCROSE-PHOSPHATE SYNTHASE IN HIGHER PLANTS.
  Journal
Annu Rev Plant Physiol Plant Mol Biol 47:431-444 (1996)
DOI:10.1146/annurev.arplant.47.1.431
Reference
4  [PMID:12062401]
  Authors
Cumino A, Curatti L, Giarrocco L, Salerno GL
  Title
Sucrose metabolism: Anabaena sucrose-phosphate synthase and sucrose-phosphate phosphatase define minimal functional domains shuffled during evolution.
  Journal
FEBS Lett 517:19-23 (2002)
DOI:10.1016/S0014-5793(02)02516-4
Reference
5  [PMID:18424616]
  Authors
Chua TK, Bujnicki JM, Tan TC, Huynh F, Patel BK, Sivaraman J
  Title
The structure of sucrose phosphate synthase from Halothermothrix orenii reveals its mechanism of action and binding mode.
  Journal
Plant Cell 20:1059-72 (2008)
DOI:10.1105/tpc.107.051193
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.14
IUBMB Enzyme Nomenclature: 2.4.1.14
ExPASy - ENZYME nomenclature database: 2.4.1.14
BRENDA, the Enzyme Database: 2.4.1.14
CAS: 9030-06-2

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