KEGG   ENZYME: 2.4.1.143Help
Entry
EC 2.4.1.143                Enzyme                                 

Name
alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase;
MGAT2 (gene name);
N-acetylglucosaminyltransferase II;
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II;
acetylglucosaminyltransferase II;
uridine diphosphoacetylglucosamine-mannoside alpha1->6-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-alpha-1,6-mannosylglycoprotein beta-1-2-N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-alpha-D-mannoside beta1-2-acetylglucosaminyltransferase;
UDP-GlcNAc:mannoside alpha1-6 acetylglucosaminyltransferase;
alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase;
GnTII;
GlcNAc-T II;
UDP-N-acetyl-D-glucosamine:6-(alpha-D-mannosyl)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 2-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] [RN:R05985]
Reaction(KEGG)
R05985(G);
(other) R04653 R07258
Show
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Product
UDP [CPD:C00015];
beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Comment
The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. Its activity initiates the synthesis of the second antenna of di-antennary complex N-glycans. While the natural substrate (produced by EC 3.2.1.114, mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) is described here, the minimal substrate recognized by the enzyme is alpha-D-Man-(1->6)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)]-beta-D-Man-R.
History
EC 2.4.1.143 created 1984, modified 2001 (EC 2.4.1.51 created 1972, part incorporated 1984), modified 2018
Pathway
ec00510  N-Glycan biosynthesis
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K00736  alpha-1,6-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Genes
HSA: 4247(MGAT2)
PTR: 740608(MGAT2)
PPS: 100985211(MGAT2)
GGO: 101130348(MGAT2)
PON: 100460559(MGAT2)
NLE: 100596086(MGAT2)
MCC: 706426(MGAT2)
MCF: 101866719(MGAT2)
CSAB: 103228939(MGAT2)
RRO: 104682761(MGAT2)
RBB: 108513657(MGAT2)
CJC: 100403225(MGAT2)
SBQ: 101052146(MGAT2)
MMU: 217664(Mgat2)
RNO: 94273(Mgat2)
CGE: 100753385(Mgat2)
NGI: 103731525(Mgat2)
HGL: 101707621(Mgat2)
CCAN: 109693265(Mgat2)
OCU: 100348676(MGAT2)
TUP: 102472350(MGAT2)
CFA: 480312(MGAT2)
AML: 100469937(MGAT2)
UMR: 103666304(MGAT2)
ORO: 101382060(MGAT2)
FCA: 101092119(MGAT2)
PTG: 102949276(MGAT2)
AJU: 106985090(MGAT2)
BTA: 101906103(MGAT2)
BOM: 102282541(MGAT2)
BIU: 109564345(MGAT2)
PHD: 102331940(MGAT2)
CHX: 102188772(MGAT2)
OAS: 101122525(MGAT2)
SSC: 100151745(MGAT2)
CFR: 102518813(MGAT2)
CDK: 105091398(MGAT2)
BACU: 103017062(MGAT2)
LVE: 103084076(MGAT2)
OOR: 101281892(MGAT2)
ECB: 100065740(MGAT2)
EPZ: 103556135(MGAT2)
EAI: 106839813(MGAT2)
MYB: 102251136(MGAT2)
MYD: 102755530(MGAT2)
HAI: 109393313(MGAT2)
RSS: 109449606(MGAT2)
PALE: 102884447(MGAT2)
LAV: 100670034(MGAT2)
TMU: 101345081
MDO: 100023862(MGAT2)
SHR: 100927464(MGAT2)
OAA: 100084500(MGAT2)
GGA: 428925(MGAT2)
MGP: 100539456(MGAT2)
CJO: 107315497(MGAT2)
APLA: 101805390(MGAT2)
ACYG: 106046961(MGAT2)
TGU: 105759302(MGAT2)
GFR: 102034157(MGAT2)
FAB: 101807768(MGAT2)
PHI: 102104535(MGAT2)
PMAJ: 107206212(MGAT2)
CCAE: 111929511(MGAT2)
CCW: 104695920(MGAT2)
FPG: 101913920(MGAT2)
FCH: 102059974(MGAT2)
CLV: 102088921(MGAT2)
EGZ: 104135015(MGAT2)
AAM: 106487024(MGAT2)
PSS: 102448885(MGAT2)
CMY: 102939955(MGAT2)
CPIC: 101942140(MGAT2)
PVT: 110070669(MGAT2)
PBI: 103052483(MGAT2)
GJA: 107122497(MGAT2)
XLA: 414590(mgat2.L) 444674(mgat2.S) 446383 734412
XTR: 448439(mgat2)
NPR: 108786864(MGAT2) 108800163
DRE: 100000478(mgat2)
IPU: 108258131(mgat2)
AMEX: 103024773(mgat2)
TRU: 101063078(mgat2) 101067570
LCO: 104924696(mgat2) 104939903
NCC: 104943934 104961827(mgat2)
MZE: 101475602(mgat2) 101487794
OLA: 101158071 101165022(mgat2)
XMA: 102219064(mgat2) 102232240
PRET: 103457757 103457913(mgat2)
NFU: 107378078(mgat2) 107378240
KMR: 108231761(mgat2) 108245450
CSEM: 103380991(mgat2) 103398574
LCF: 108895810 108896893(mgat2)
SDU: 111238109 111240326(mgat2)
HCQ: 109531026(mgat2) 109531778
BPEC: 110159416 110168298(mgat2)
MALB: 109962268(mgat2) 109969812
ELS: 105017600 105030576(mgat2)
SFM: 108921073 108941417(mgat2)
LCM: 102345092 102367380(MGAT2)
CMK: 103175354(mgat2) 103177928
CIN: 104265636
SPU: 593053
APLC: 110985093
SKO: 100371978
DME: Dmel_CG7921(Mgat2)
DER: 6554185
DSI: Dsimw501_GD17443(Dsim_GD17443)
DWI: 6651706
AAG: 5567292
AME: 726476
BIM: 100740734
BTER: 100648803
SOC: 105196388
AEC: 105142912
ACEP: 105621431
PBAR: 105428964
HST: 105187914
DQU: 106748266
CFO: 105250520
LHU: 105674886
PGC: 109859540
PCF: 106786134
NVI: 103315305
MDL: 103575871
TCA: 658173
NVL: 108563072
BMOR: 101735304
PMAC: 106709226
PRAP: 111002058
DNX: 107162987
CLEC: 106666176
ZNE: 110834632
FCD: 110862032
TUT: 107360018
CEL: CELE_C03E10.4(gly-20)
CBR: CBG23158(Cbr-gly-20)
BMY: Bm1_39220
TSP: Tsp_00241
CRG: 105331306
MYI: 110462356
OBI: 106880532
LAK: 106168558
SHX: MS3_08042
EGL: EGR_00151
ADF: 107338785
HMG: 100199919
ATH: AT2G05320
CRB: 17893574
BRP: 103860332
BOE: 106336243
THJ: 104808847
CPAP: 110808688
CIT: 102618199
TCC: 18612481
EGR: 104456601
GMX: 100819421
VRA: 106769957
VAR: 108324991
CCAJ: 109794988
CAM: 101500842
LJA: Lj5g3v1974400.1(Lj5g3v1974400.1) Lj6g3v0098640.1(Lj6g3v0098640.1)
ADU: 107477874
AIP: 107629873
LANG: 109325898
FVE: 101309374
PPER: 18783149
PMUM: 103329256
PAVI: 110749976
CSV: 101213679
CMO: 103500510
MCHA: 111007879
CMAX: 111468194
CMOS: 111455025
RCU: 8264003
JCU: 105641508
HBR: 110668339
POP: 7456009
JRE: 108994044
VVI: 100240895
SLY: 101259650
SPEN: 107026462
SOT: 102596137
CANN: 107878105
NSY: 104214949
NTO: 104091096
INI: 109188318
OEU: 111370345
HAN: 110889682
LSV: 111900476
CCAV: 112508227
DCR: 108219797
BVG: 104903002
SOE: 110805451
OSA: 9266501
DOSA: Os02t0798100-00(Os02g0798100)
OBR: 102702352
BDI: 100832652
ATS: 109779500(LOC109779500)
ZMA: 100381400
SITA: 101755999
PDA: 103700877
EGU: 105033217
PEQ: 110033504
AOF: 109849873
ATR: 18446103
SMO: SELMODRAFT_80130(GntII)
 » show all
Taxonomy
Reference
1  [PMID:6445358]
  Authors
Harpaz N, Schachter H.
  Title
Control of glycoprotein synthesis. Bovine colostrum UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I. Separation from UDP-N-acetylglucosamine:alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase II, partial purification, and substrate specificity.
  Journal
J Biol Chem 255:4885-93 (1980)
Reference
2  [PMID:6452163]
  Authors
Mendicino J, Chandrasekaran EV, Anumula KR, Davila M.
  Title
Isolation and properties of alpha-D-mannose:beta-1,2-N-acetylglucosaminyltransferase from trachea mucosa.
  Journal
Biochemistry 20:967-76 (1981)
Reference
3  [PMID:6457827]
  Authors
Oppenheimer CL, Eckhardt AE, Hill RL.
  Title
The nonidentity of porcine N-acetylglucosaminyltransferases I and II.
  Journal
J Biol Chem 256:11477-82 (1981)
Reference
4  [PMID:6366476]
  Authors
Schachter H, Narasimhan S, Gleeson P, Vella G.
  Title
Glycosyltransferases involved in elongation of N-glycosidically linked oligosaccharides of the complex or N-acetyllactosamine type.
  Journal
Methods Enzymol 98:98-134 (1983)
Reference
5  [PMID:2952645]
  Authors
Bendiak B, Schachter H
  Title
Control of glycoprotein synthesis. Kinetic mechanism, substrate specificity, and  inhibition characteristics of UDP-N-acetylglucosamine:alpha-D-mannoside beta 1-2  N-acetylglucosaminyltransferase II from rat liver.
  Journal
J Biol Chem 262:5784-90 (1987)
  Sequence
[rno:94273]
Reference
6  [PMID:2952644]
  Authors
Bendiak B, Schachter H.
  Title
Control of glycoprotein synthesis. Purification of UDP-N-acetylglucosamine:alpha-D-mannoside beta 1-2 N-acetylglucosaminyltransferase II from rat liver.
  Journal
J Biol Chem 262:5775-83 (1987)
Reference
7  [PMID:7635144]
  Authors
Tan J, D'Agostaro AF, Bendiak B, Reck F, Sarkar M, Squire JA, Leong P, Schachter H
  Title
The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2- N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of  the recombinant protein.
  Journal
Eur J Biochem 231:317-28 (1995)
DOI:10.1111/j.1432-1033.1995.0317e.x
  Sequence
[hsa:4247]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.143
IUBMB Enzyme Nomenclature: 2.4.1.143
ExPASy - ENZYME nomenclature database: 2.4.1.143
BRENDA, the Enzyme Database: 2.4.1.143
CAS: 105913-04-0

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