KEGG   ENZYME: 2.4.1.146Help
Entry
EC 2.4.1.146                Enzyme                                 

Name
beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase;
O-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II;
uridine diphosphoacetylglucosamine-mucin beta(1->3)-acetylglucosaminyltransferase (elongating);
elongation 3beta-GalNAc-transferase;
UDP-N-acetyl-D-glucosamine:O-glycosyl-glycoprotein (N-acetyl-D-glucosamine to beta-D-galactose of beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R) beta-1,3-N-acetyl-D-glucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->3)-[N-acetyl-D-glucosaminyl-(1->6)]-N-acetyl-D-galactosaminyl-R 3-beta-N-acetyl-D-glucosaminyltransferase;
B3GNT3 (gene name)
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] 3-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + 3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] = UDP + 3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein] [RN:R04636 R05901]
Reaction(KEGG)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
3-O-{beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]
Product
UDP [CPD:C00015];
3-O-{N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->3)-[N-acetyl-beta-D-glucosaminyl-(1->6)]-N-acetyl-alpha-D-galactosaminyl}-L-seryl/threonyl-[protein]
Comment
The enzyme catalyses the addition of N-acetyl-alpha-D-glucosamine to the core 2 structure of O-glycans.
History
EC 2.4.1.146 created 1984, modified 2018
Orthology
K07970  beta-1,3-N-acetylglucosaminyltransferase 3
Genes
HSA: 10331(B3GNT3)
PTR: 455847(B3GNT3)
PPS: 100982986(B3GNT3)
GGO: 101151557(B3GNT3)
PON: 100433243(B3GNT3)
NLE: 100596245(B3GNT3)
MCC: 719221(B3GNT3)
MCF: 102134304(B3GNT3)
CSAB: 103234153(B3GNT3)
RRO: 104660961(B3GNT3)
RBB: 108534279(B3GNT3)
CJC: 100404385(B3GNT3)
SBQ: 101033879(B3GNT3)
MMU: 72297(B3gnt3)
RNO: 290638(B3gnt3)
CGE: 100773213(B3gnt3)
NGI: 103726889(B3gnt3)
HGL: 101713138(B3gnt3)
CCAN: 109698339(B3gnt3)
TUP: 102472378(B3GNT3) 102476277
CFA: 484831(B3GNT3)
AML: 100473756(B3GNT3)
UMR: 103675168(B3GNT3)
ORO: 101368545(B3GNT3)
FCA: 101092089(B3GNT3)
PTG: 102965987(B3GNT3)
AJU: 106969093(B3GNT3)
BTA: 784997(B3GNT3)
BOM: 102281061(B3GNT3)
BIU: 109560822(B3GNT3)
CHX: 102171777(B3GNT3)
OAS: 101113066(B3GNT3)
SSC: 100518473(B3GNT3)
CFR: 102512477(B3GNT3)
CDK: 105101856(B3GNT3)
BACU: 103012961(B3GNT3)
LVE: 103080965(B3GNT3)
OOR: 101282171(B3GNT3)
ECB: 100146466(B3GNT3)
EPZ: 103542315(B3GNT3)
EAI: 106830835(B3GNT3)
MYB: 102254606(B3GNT3)
MYD: 102767713(B3GNT3)
HAI: 109382386(B3GNT3)
RSS: 109450104(B3GNT3)
PALE: 102882932(B3GNT3)
LAV: 100673587(B3GNT3)
MDO: 100010637(B3GNT3)
SHR: 100920625(B3GNT3)
OAA: 100086714
MGP: 100549756(B3GNT3)
CJO: 107325454(B3GNT3)
APLA: 101789872(B3GNT3)
ACYG: 106048207(B3GNT3)
FPG: 106112886(B3GNT3)
FCH: 102054592(B3GNT3)
CLV: 102095902(B3GNT3)
EGZ: 104124478(B3GNT3)
AAM: 106486607
ASN: 102373956(B3GNT3)
AMJ: 106737906(B3GNT3)
PSS: 102443521(B3GNT3)
CMY: 102944348(B3GNT3)
CPIC: 101936651(B3GNT3)
PVT: 110072506(B3GNT3)
PBI: 103061136(B3GNT3)
GJA: 107120629(B3GNT3)
XTR: 100145701(b3gnt3) 100494334
DRE: 100002020(b3gnt3.2) 561172(b3gnt3.4)
SRX: 107755313
SANH: 107665060 107700302(b3gnt3)
SGH: 107599054
AMEX: 103026573(b3gnt3)
TRU: 101072648(b3gnt3)
NCC: 104958016
SDU: 111218271(b3gnt3) 111235274
HCQ: 109519123(b3gnt3)
BPEC: 110160181
MALB: 109958356
OTW: 112250760
SFM: 108930515(b3gnt3)
LCM: 102345207(B3GNT3)
CMK: 103181970(b3gnt3)
 » show all
Taxonomy
Reference
1  [PMID:6226356]
  Authors
Brockhausen I, Rachaman ES, Matta KL, Schachter H.
  Title
The separation by liquid chromatography (under elevated pressure) of phenyl, benzyl, and O-nitrophenyl glycosides of oligosaccharides. Analysis of substrates and products for four N-acetyl-D-glucosaminyl-transferases involved in mucin synthesis.
  Journal
Carbohydr Res 120:3-16 (1983)
DOI:10.1016/0008-6215(83)88002-1
Reference
2  [PMID:11042166]
  Authors
Shiraishi N, Natsume A, Togayachi A, Endo T, Akashima T, Yamada Y, Imai N, Nakagawa S, Koizumi S, Sekine S, Narimatsu H, Sasaki K.
  Title
Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family.
  Journal
J Biol Chem 276:3498-507 (2001)
DOI:10.1074/jbc.M004800200
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.146
IUBMB Enzyme Nomenclature: 2.4.1.146
ExPASy - ENZYME nomenclature database: 2.4.1.146
BRENDA, the Enzyme Database: 2.4.1.146
CAS: 87927-99-9

KEGG   ENZYME: 2.4.1.149Help
Entry
EC 2.4.1.149                Enzyme                                 

Name
N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-acetyllactosaminide beta1->3-acetylglucosaminyltransferase;
poly-N-acetyllactosamine extension enzyme;
Galbeta1->4GlcNAc-R beta1->3 N-acetylglucosaminyltransferase;
UDP-GlcNAc:GalR beta-D-3-N-acetylglucosaminyltransferase;
N-acetyllactosamine beta(1-3)N-acetylglucosaminyltransferase;
UDP-GlcNAc:Galbeta1->4GlcNAcbeta-Rbeta1->3-N-acetylglucosaminyltransferase;
GnTE;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-1,4-N-acetyl-D-glucosamine beta-1,3-acetyl-D-glucosaminyltransferase;
beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase;
UDP-N-acetyl-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine 3-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R 3-beta N-acetylglucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R [RN:R02789]
Reaction(KEGG)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R [CPD:C00694]
Product
UDP [CPD:C00015];
N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R [CPD:C04890]
Comment
Acts on beta-galactosyl-1,4-N-acetylglucosaminyl termini on glycoproteins, glycolipids, and oligosaccharides.
History
EC 2.4.1.149 created 1984 (EC 2.4.1.163 created 1989, incorporated 2016), modified 2016
Pathway
ec00533  Glycosaminoglycan biosynthesis - keratan sulfate
ec00601  Glycosphingolipid biosynthesis - lacto and neolacto series
ec01100  Metabolic pathways
Orthology
K00741  N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase
K07970  beta-1,3-N-acetylglucosaminyltransferase 3
K07971  beta-1,3-N-acetylglucosaminyltransferase 4
Genes
HSA: 10331(B3GNT3) 10678(B3GNT2) 79369(B3GNT4)
PTR: 455847(B3GNT3) 744166(B3GNT4) 745838(B3GNT2)
PPS: 100973625(B3GNT2) 100982200(B3GNT4) 100982986(B3GNT3)
GGO: 101126374(B3GNT4) 101130700(B3GNT2) 101151557(B3GNT3)
PON: 100433243(B3GNT3) 100437574(B3GNT4) 100461200(B3GNT2)
NLE: 100585350(B3GNT4) 100596245(B3GNT3) 100607325(B3GNT2)
MCC: 693396(B3GNT2) 702023(B3GNT4) 719221(B3GNT3)
MCF: 102134304(B3GNT3) 102136094(B3GNT2) 102138868(B3GNT4)
CSAB: 103220181(B3GNT2) 103234153(B3GNT3) 103239303(B3GNT4)
RRO: 104660961(B3GNT3) 104666296(B3GNT4) 104666963(B3GNT2)
RBB: 108517019(B3GNT2) 108534279(B3GNT3) 108541058(B3GNT4)
CJC: 100385332 100402866(LRRC43) 100404385(B3GNT3) 100407247(B3GNT2)
SBQ: 101033879(B3GNT3) 101041127(B3GNT4) 101043174(B3GNT2) 104650627
MMU: 231727(B3gnt4) 53625(B3gnt2) 72297(B3gnt3)
RNO: 288752(B3gnt4) 290638(B3gnt3) 305571(B3gnt2)
CGE: 100766691(B3gnt2) 100769077(B3gnt4) 100773213(B3gnt3)
NGI: 103726889(B3gnt3) 103739056(B3gnt4) 103743255(B3gnt2)
HGL: 101697436(B3gnt4) 101713138(B3gnt3) 101714518 101723595(B3gnt2)
CCAN: 109684612(B3gnt4) 109698339(B3gnt3) 109698721(B3gnt2)
OCU: 100342947(B3GNT2) 103345065(B3GNT4)
TUP: 102471847(B3GNT2) 102472378(B3GNT3) 102476277 102485148(B3GNT4)
CFA: 474611(B3GNT2) 484831(B3GNT3) 486255(B3GNT4)
AML: 100473756(B3GNT3) 100480301(B3GNT4) 100484275(B3GNT2)
UMR: 103661263(B3GNT4) 103671308(B3GNT2) 103675168(B3GNT3)
ORO: 101368545(B3GNT3) 101372054(B3GNT4) 101381726(B3GNT2)
FCA: 101080523(B3GNT2) 101092089(B3GNT3) 101092795(B3GNT4)
PTG: 102953748(B3GNT2) 102965987(B3GNT3) 102969555(B3GNT4)
AJU: 106967555(B3GNT4) 106969093(B3GNT3) 106973472(B3GNT2)
BTA: 515585(B3GNT2) 534619(B3GNT4) 784997(B3GNT3)
BOM: 102270553 102277608 102278923(B3GNT4) 102281061(B3GNT3) 102285789(B3GNT2)
BIU: 109560124 109560822(B3GNT3) 109566200(B3GNT2) 109568239 109571378(B3GNT4)
PHD: 102337098(B3GNT2) 102344674(B3GNT4)
CHX: 102168846(B3GNT4) 102171777(B3GNT3) 102172908(B3GNT2)
OAS: 101102267(B3GNT4) 101113066(B3GNT3) 101117403(B3GNT2)
SSC: 100518473(B3GNT3) 100524195(B3GNT2) 100736645(B3GNT4)
CFR: 102506509(B3GNT2) 102512477(B3GNT3) 102519695(B3GNT4)
CDK: 105097252(B3GNT2) 105101856(B3GNT3) 105102523(B3GNT4)
BACU: 103010834(B3GNT4) 103012961(B3GNT3) 103013589(B3GNT2) 103018714
LVE: 103079240(B3GNT2) 103080965(B3GNT3) 103086312(B3GNT4)
OOR: 101277117(B3GNT4) 101282171(B3GNT3) 101290494(B3GNT2)
ECB: 100060158(B3GNT4) 100064405(B3GNT2) 100146466(B3GNT3)
EPZ: 103541201(B3GNT4) 103542315(B3GNT3) 103566217(B3GNT2)
EAI: 106828376(B3GNT2) 106830835(B3GNT3) 106842309(B3GNT4)
MYB: 102254606(B3GNT3) 102258133(B3GNT4) 106723541(B3GNT2)
MYD: 102762333(B3GNT2) 102767713(B3GNT3) 102772504(B3GNT4)
HAI: 109377910(B3GNT2) 109382386(B3GNT3) 109386073(B3GNT4)
RSS: 109438062(B3GNT2) 109448569(B3GNT4) 109450104(B3GNT3)
PALE: 102879999(B3GNT2) 102882932(B3GNT3) 102898492(B3GNT4)
LAV: 100656431(B3GNT2) 100667062(B3GNT4) 100673587(B3GNT3)
MDO: 100010637(B3GNT3) 100022055(B3GNT4) 100031891(B3GNT2)
SHR: 100920625(B3GNT3) 100928418(B3GNT2) 100929127(B3GNT4)
OAA: 100074841(B3GNT4) 100078191(B3GNT2) 100086714
GGA: 100858043(B3GNT4) 421286(B3GNT2)
MGP: 100540421(B3GNT2) 100545749(B3GNT4) 100549756(B3GNT3)
CJO: 107310873(B3GNT2) 107321114(B3GNT4) 107325454(B3GNT3)
APLA: 101789872(B3GNT3) 101793948(B3GNT2) 101804408(B3GNT4)
ACYG: 106031126(B3GNT4) 106045606(B3GNT2) 106048207(B3GNT3)
TGU: 100217506(B3GNT2) 100226631(B3GNT4)
GFR: 102031557(B3GNT4) 102038254(B3GNT2)
FAB: 101811159(B3GNT4) 101811829(B3GNT2)
PHI: 102101528(B3GNT4) 102107608(B3GNT2)
PMAJ: 107202442(B3GNT2) 107211771(B3GNT4)
CCAE: 111926547(B3GNT2) 111936404(B3GNT4)
CCW: 104688690(B3GNT4) 104696618(B3GNT2)
FPG: 101910333(B3GNT2) 101917583(B3GNT4) 106112886(B3GNT3)
FCH: 102047111(B3GNT2) 102054592(B3GNT3) 102054954(B3GNT4)
CLV: 102085751(B3GNT2) 102087378(B3GNT4) 102095902(B3GNT3)
EGZ: 104122740(B3GNT4) 104124478(B3GNT3) 104129481(B3GNT2)
AAM: 106486607 106495676(B3GNT4) 106499878(B3GNT2)
ASN: 102370599(B3GNT2) 102373956(B3GNT3) 102377528(B3GNT4)
AMJ: 102572682(B3GNT4) 102573235(B3GNT2) 106737906(B3GNT3)
PSS: 102443521(B3GNT3) 102444677(B3GNT4) 102453681 102460724(B3GNT2)
CMY: 102933292 102935807(B3GNT4) 102936051(B3GNT2) 102944348(B3GNT3)
CPIC: 101935467(B3GNT4) 101936651(B3GNT3) 101937115 101943630(B3GNT2) 103305821
ACS: 100554272(b3gnt2) 100566391(b3gnt4)
PVT: 110072506(B3GNT3) 110077488(B3GNT2) 110083863(B3GNT4)
PBI: 103052938(B3GNT2) 103061136(B3GNT3)
GJA: 107115872(B3GNT4) 107120629(B3GNT3) 107121952(B3GNT2)
XLA: 108703715 108705729(b3gnt4.S) 108711807 108716744(b3gnt4.L) 414647(b3gnt2.L) 446358(b3gnt2.S) 734264
XTR: 100145701(b3gnt3) 100170561(b3gnt2) 100491670(b3gnt4) 100494334
DRE: 100002020(b3gnt3.2) 561172(b3gnt3.4) 793362(b3gnt2b)
TRU: 101061479 101071706 101072648(b3gnt3) 101079085(b3gnt2)
HCQ: 109515786 109518316 109519123(b3gnt3) 109526674(b3gnt2)
SFM: 108920231 108921069 108930515(b3gnt3) 108933745(b3gnt2) 108934765(b3gnt4)
LCM: 102345207(B3GNT3) 102350925 102359554(B3GNT2) 102362021(B3GNT4) 106704705
BMY: Bm1_50875
CRG: 105333708
 » show all
Taxonomy
Reference
1  [PMID:6219989]
  Authors
van den Eijnden DH, Winterwerp H, Smeeman P, Schiphorst WE.
  Title
Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity.
  Journal
J Biol Chem 258:3435-7 (1983)
Reference
2  [PMID:6238026]
  Authors
Basu M, Basu S.
  Title
Biosynthesis in vitro of Ii core glycosphingolipids from neolactotetraosylceramide by beta 1-3- and beta 1-6-N-acetylglucosaminyltransferases from mouse T-lymphoma.
  Journal
J Biol Chem 259:12557-62 (1984)
Reference
3  [PMID:3160874]
  Authors
Takeya A, Hosomi O, Kogure T.
  Title
The presence of N-acetyllactosamine and lactose: beta (1-3)N-acetylglucosaminyltransferase activity in human urine.
  Journal
Jpn J Med Sci Biol 38:1-8 (1985)
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.149
IUBMB Enzyme Nomenclature: 2.4.1.149
ExPASy - ENZYME nomenclature database: 2.4.1.149
BRENDA, the Enzyme Database: 2.4.1.149
CAS: 85638-39-7

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