KEGG   ENZYME: 2.4.1.155Help
Entry
EC 2.4.1.155                Enzyme                                 

Name
alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase;
MGAT5 (gene name);
N-acetylglucosaminyltransferase V;
alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase;
uridine diphosphoacetylglucosamine-alpha-mannoside beta1->6-acetylglucosaminyltransferase;
UDP-N-acetylglucosamine:alpha-mannoside-beta1,6 N-acetylglucosaminyltransferase;
alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetylglucosaminyltransferase;
GnTV;
GlcNAc-T V;
UDP-N-acetyl-D-glucosamine:6-[2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl]-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)-beta-D-mannosyl-glycoprotein 6-beta-N-acetyl-D-glucosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] [RN:R05991]
Reaction(KEGG)
R05991(G);
(other) R04665
Show
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Product
UDP [CPD:C00015];
beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein]
Comment
Requires Mg2+. The enzyme, found in vertebrates, participates in the processing of N-glycans in the Golgi apparatus. It catalyses the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides, and thus enables the synthesis of tri- and tetra-antennary complexes.
History
EC 2.4.1.155 created 1986, modified 2001, modified 2018
Pathway
ec00510  N-Glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K00744  alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetyl-glucosaminyltransferase
Genes
HSA: 4249(MGAT5)
PTR: 459625(MGAT5)
PPS: 100989629(MGAT5)
GGO: 101129003(MGAT5)
PON: 100445523(MGAT5)
NLE: 100597446(MGAT5)
MCC: 709108(MGAT5)
MCF: 102123398(MGAT5)
CSAB: 103216975(MGAT5)
RRO: 104654400(MGAT5) 104676211
RBB: 108539646(MGAT5)
CJC: 100414526(MGAT5)
SBQ: 101038866(MGAT5)
MMU: 107895(Mgat5)
RNO: 65271(Mgat5)
CGE: 100760162(Mgat5)
NGI: 103733594(Mgat5)
HGL: 101721991(Mgat5)
OCU: 100346879(MGAT5)
TUP: 102497975(MGAT5)
CFA: 483895(MGAT5)
AML: 100483104(MGAT5)
UMR: 103667662(MGAT5)
ORO: 101379628(MGAT5)
FCA: 101088682(MGAT5)
PTG: 102969054(MGAT5)
AJU: 106983003(MGAT5)
BTA: 537595(MGAT5)
BOM: 102275628(MGAT5)
BIU: 109568385(MGAT5)
PHD: 102335781(MGAT5)
CHX: 102174983(MGAT5)
OAS: 101107672(MGAT5)
SSC: 100512563(MGAT5)
CFR: 102521418(MGAT5)
CDK: 105092441(MGAT5)
BACU: 102997655(MGAT5)
LVE: 103072240(MGAT5)
OOR: 101288542(MGAT5)
ECB: 100050356(MGAT5)
EPZ: 103555913(MGAT5)
EAI: 106836533(MGAT5)
MYB: 102246850(MGAT5)
MYD: 102761898(MGAT5)
HAI: 109374684(MGAT5)
RSS: 109447110(MGAT5)
PALE: 102884190(MGAT5)
LAV: 100664057(MGAT5)
TMU: 101345363
MDO: 100011845(MGAT5)
SHR: 100929992(MGAT5)
OAA: 100091600(MGAT5)
GGA: 424286(MGAT5)
MGP: 100541307(MGAT5)
CJO: 107316876(MGAT5)
APLA: 101797784(MGAT5)
ACYG: 106039337(MGAT5)
TGU: 100228988(MGAT5)
GFR: 102042048(MGAT5)
FAB: 101814359(MGAT5)
PHI: 102101886(MGAT5)
PMAJ: 107207494(MGAT5)
CCAE: 111932311(MGAT5) 111941973
CCW: 104694065(MGAT5)
FPG: 101918414(MGAT5)
FCH: 102055854(MGAT5)
CLV: 102084918(MGAT5)
EGZ: 104129527(MGAT5)
AAM: 106498453(MGAT5)
ASN: 102386992(MGAT5)
AMJ: 102563315(MGAT5)
PSS: 102455461(MGAT5)
CMY: 102946323(MGAT5)
CPIC: 101938965(MGAT5)
ACS: 100566008(mgat5)
PVT: 110071860(MGAT5)
PBI: 103063095(MGAT5) 103064781
GJA: 107108668(MGAT5) 107111387
XTR: 100145551(mgat5)
NPR: 108792298(MGAT5)
DRE: 724006(mgat5)
SGH: 107586987 107593035(mgat5)
IPU: 108259240(mgat5)
AMEX: 103037113(mgat5)
LCO: 104925340(mgat5) 109142334
MZE: 101481161(mgat5)
OLA: 101168186(mgat5)
XMA: 102232481(mgat5) 102236488
PRET: 103460330 103476254(mgat5)
NFU: 107381041 107390173(mgat5)
KMR: 108238485 108246486(mgat5)
CSEM: 103389683(mgat5)
SDU: 111218827 111222699(mgat5)
HCQ: 109514989(mgat5)
BPEC: 110153275(mgat5)
MALB: 109955689(mgat5) 109968552
ELS: 105019667(mgat5)
SFM: 108929747 108941965(mgat5)
LCM: 102363158(MGAT5)
CMK: 103180379(mgat5) 103184928(ccdc126)
CIN: 100175898
SPU: 100888694
APLC: 110989436
SKO: 100366560
CEL: CELE_C55B7.2(gly-2)
CBR: CBG12645(Cbr-gly-2)
BMY: Bm1_56690
TSP: Tsp_11593
CRG: 105330629
MYI: 110458649
SHX: MS3_04856
 » show all
Taxonomy
Reference
1  [PMID:6216250]
  Authors
Cummings RD, Trowbridge IS, Kornfeld S.
  Title
A mouse lymphoma cell line resistant to the leukoagglutinating lectin from Phaseolus vulgaris is deficient in UDP-GlcNAc: alpha-D-mannoside beta 1,6 N-acetylglucosaminyltransferase.
  Journal
J Biol Chem 257:13421-7 (1982)
Reference
2  [PMID:2834054]
  Authors
Hindsgaul O, Tahir SH, Srivastava OP, Pierce M.
  Title
The trisaccharide beta-D-GlcpNAc-(1----2)-alpha-D-Manp-(1----6)-beta-D-Manp, as its 8-methoxycarbonyloctyl glycoside, is an acceptor selective for N-acetylglucosaminyltransferase V.
  Journal
Carbohydr Res 173:263-72 (1988)
DOI:10.1016/S0008-6215(00)90822-X
Reference
3  [PMID:1531335]
  Authors
Shoreibah MG, Hindsgaul O, Pierce M
  Title
Purification and characterization of rat kidney UDP-N-acetylglucosamine: alpha-6-D-mannoside beta-1,6-N-acetylglucosaminyltransferase.
  Journal
J Biol Chem 267:2920-7 (1992)
Reference
4  [PMID:8393437]
  Authors
Gu J, Nishikawa A, Tsuruoka N, Ohno M, Yamaguchi N, Kangawa K, Taniguchi N
  Title
Purification and characterization of UDP-N-acetylglucosamine: alpha-6-D-mannoside beta 1-6N-acetylglucosaminyltransferase (N-acetylglucosaminyltransferase V) from  a human lung cancer cell line.
  Journal
J Biochem 113:614-9 (1993)
Reference
5  [PMID:10395745]
  Authors
Park C, Jin UH, Lee YC, Cho TJ, Kim CH
  Title
Characterization of UDP-N-acetylglucosamine:alpha-6-d-mannoside beta-1,6-N-acetylglucosaminyltransferase V from a human hepatoma cell line Hep3B.
  Journal
Arch Biochem Biophys 367:281-8 (1999)
DOI:10.1006/abbi.1999.1252
  Sequence
[hsa:4249]
Reference
6  [PMID:11872751]
  Authors
Saito T, Miyoshi E, Sasai K, Nakano N, Eguchi H, Honke K, Taniguchi N
  Title
A secreted type of beta 1,6-N-acetylglucosaminyltransferase V (GnT-V) induces tumor angiogenesis without mediation of glycosylation: a novel function of GnT-V  distinct from the original glycosyltransferase activity.
  Journal
J Biol Chem 277:17002-8 (2002)
DOI:10.1074/jbc.M200521200
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.155
IUBMB Enzyme Nomenclature: 2.4.1.155
ExPASy - ENZYME nomenclature database: 2.4.1.155
BRENDA, the Enzyme Database: 2.4.1.155
CAS: 83588-90-3

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