KEGG   ENZYME: 2.4.1.175Help
Entry
EC 2.4.1.175                Enzyme                                 

Name
glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase;
N-acetylgalactosaminyltransferase II;
UDP-N-acetyl-D-galactosamine:D-glucuronyl-N-acetyl-1,3-beta-D-galactosaminylproteoglycan beta-1,4-N-acetylgalactosaminyltransferase;
chondroitin synthase;
glucuronyl-N-acetylgalactosaminylproteoglycan beta-1,4-N-acetylgalactosaminyltransferase;
uridine diphosphoacetylgalactosamine-chondroitin acetylgalactosaminyltransferase II;
UDP-N-acetyl-D-galactosamine:beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase;
UDP-N-acetyl-alpha-D-galactosamine:beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-galactosamine:[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine 4-beta-N-acetylgalactosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
(1) UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [RN:R04603 R05932];
(2) UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n+1-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Reaction(KEGG)
R04603 R05932(G);
(other) R05934(G)
Show
Substrate
UDP-N-acetyl-alpha-D-galactosamine [CPD:C00203];
[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [CPD:C04800];
[protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Product
UDP [CPD:C00015];
[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [CPD:C04913];
[protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n+1-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Comment
Involved in the biosynthesis of chondroitin sulfate. The human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase (EC 2.4.1.226, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase) activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase 'co-polymerases' can be found in Pasteurella multocida and Escherichia coli.
History
EC 2.4.1.175 created 1989, modified 2002
Pathway
ec00532  Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
ec01100  Metabolic pathways
Orthology
K00746  chondroitin sulfate N-acetylgalactosaminyltransferase 1/2
K00747  chondroitin polymerizing factor
K13499  chondroitin sulfate synthase
K13500  chondroitin synthase
Genes
HSA: 22856(CHSY1) 337876(CHSY3) 55454(CSGALNACT2) 55790(CSGALNACT1) 79586(CHPF)
PTR: 450416(CSGALNACT2) 464030(CSGALNACT1) 467777(CHSY1) 471623(CHSY3) 743513(CHPF)
PPS: 100968775(CHSY1) 100971582(CHPF) 100972801(CHSY3) 100974901 100987599(CSGALNACT1) 100994377(CSGALNACT2)
GGO: 101126045(CHSY3) 101136357(CHSY1) 101144411(CHPF) 101151962(CSGALNACT2) 101154228(CSGALNACT1)
PON: 100172853(CSGALNACT2) 100442293(CSGALNACT1) 100449809(CHSY1) 103888550(CHPF)
NLE: 100583001(CSGALNACT1) 100588681(CHSY3) 100589763(CHPF) 100602432(CSGALNACT2) 100602507(CHSY1)
MCC: 694481(CHSY1) 703703(CSGALNACT2) 703860(CHPF) 704901(CSGALNACT1) 705715(CHSY3)
MCF: 101866114(CHPF) 102125913(CSGALNACT1) 102136125(CHSY1) 102136240(CHSY3) 102136560(CSGALNACT2)
CSAB: 103215414(CSGALNACT1) 103215756(CSGALNACT2) 103217919(CHPF) 103231288(CHSY1) 103244479(CHSY3)
RRO: 104653929(CSGALNACT2) 104660068(CHPF) 104662459(CSGALNACT1) 104676683(CHSY3) 104676811(CHSY1)
RBB: 108513317(CSGALNACT2) 108518435(CHPF) 108522937(CHSY1) 108526479(CSGALNACT1) 108531341(CHSY3)
CJC: 100392140(CHSY3) 100399122(CSGALNACT2) 100403329(CHPF) 100405485(CHSY1) 100407007(CSGALNACT1)
SBQ: 101027448(CSGALNACT2) 101046087(CHPF) 101047506(CHSY3) 101048885(CSGALNACT1) 101054458(CHSY1)
MMU: 234356(Csgalnact1) 269941(Chsy1) 74241(Chpf) 78752(Csgalnact2) 78923(Chsy3)
RNO: 100910780(Chsy3l) 291577(Chsy3) 292999(Chsy1) 297554(Csgalnact2) 306375(Csgalnact1) 316533(Chpf)
CGE: 100754969(Csgalnact1) 100765856(Chpf) 100767985(Chsy3) 100770803(Chsy1) 100770830(Csgalnact2)
NGI: 103727760(Chpf) 103733802(Csgalnact2) 103747203(Chsy1) 103747604(Csgalnact1) 103751045(Chsy3)
HGL: 101700301(Chsy1) 101700905(Csgalnact1) 101701741(Chsy3) 101707555(Chpf) 101719048(Csgalnact2)
CCAN: 109673993 109683396 109685049(Chsy1) 109688882(Csgalnact1) 109695115(Chpf) 109698852(Csgalnact2)
OCU: 100328892(CHPF) 100339411(CSGALNACT1) 100339435(CSGALNACT2) 100351896(CHSY3) 103351288(CHSY1)
TUP: 102468989(CSGALNACT2) 102488411(CHPF) 102490797(CSGALNACT1) 102491913(CHSY3) 102495682(CHSY1)
CFA: 481492(CHSY3) 482831(CSGALNACT1) 486786(CSGALNACT2) 488539(CHPF) 488704(CHSY1)
AML: 100466495(CHSY1) 100468746(CSGALNACT1) 100484507(CHSY3) 100484755(CSGALNACT2) 105240013(CHPF)
UMR: 103660037(CHSY1) 103662872(CHPF) 103667936(CSGALNACT2) 103670570(CHSY3) 103677036(CSGALNACT1)
ORO: 101369554(CSGALNACT2) 101377082(CSGALNACT1) 101380609(CHSY1) 101382953(CHSY3) 101383392(CHPF)
FCA: 101083179(CHSY1) 101096311(CSGALNACT1) 101097752(CHPF) 101100841(CSGALNACT2) 105260677 111556250(CHSY3)
PTG: 102952320(CHSY1) 102956824(CSGALNACT1) 102959394(CHPF) 102966347(CSGALNACT2) 102971026(CHSY3)
AJU: 106970041(CHPF) 106970692(CHSY3) 106972094(CHSY1) 106972130(CSGALNACT2) 106978257(CSGALNACT1)
BTA: 281690(CHSY1) 509328(CSGALNACT2) 528057(CSGALNACT1) 528149(CHSY3) 540002(CHPF)
BOM: 102266543(CSGALNACT1) 102281638(CHSY1) 102281650(CHSY3) 102284379(CHPF) 102285203(CSGALNACT2)
BIU: 109553356(CSGALNACT1) 109553754(CSGALNACT2) 109561832(CHSY3) 109571205(CHPF) 109575124(CHSY1)
PHD: 102319423(CHPF) 102326020 102327197 102330279(CSGALNACT2) 102333649(CSGALNACT1) 102334956 102344003(CHSY1)
CHX: 102170413(CHSY3) 102179324(CHSY1) 102179349(CSGALNACT1) 102181823(CHPF) 102185714(CSGALNACT2)
OAS: 101104485(CHSY3) 101109305(CHSY1) 101114088(CHPF) 101118243(CSGALNACT2) 101120469 105605252
SSC: 100157878(CSGALNACT2) 100517735 100518720(CHSY3) 100520288(CHSY1) 100623047(CHPF) 102166124(CSGALNACT1)
CFR: 102504589(CHSY3) 102513810 102514190(CSGALNACT2) 102514921(CSGALNACT1) 102523829(CHSY1) 102524541(CHPF)
CDK: 105086444(CSGALNACT1) 105089922(CHSY3) 105092509(CSGALNACT2) 105093456(CHSY1) 105096119(CHPF)
BACU: 103007226(CSGALNACT2) 103010484(CSGALNACT1) 103011106(CHPF) 103012595(CHSY1) 103019431(CHSY3)
LVE: 103069443(CSGALNACT2) 103069960(CHPF) 103072590(CHSY3) 103082442(CHSY1) 103083412 103084873(CSGALNACT1)
OOR: 101279931(CHPF) 101283608(CHSY3) 101287224(CHSY1) 101288535(CSGALNACT2) 101289782(CSGALNACT1)
ECB: 100050200(CSGALNACT2) 100052192(CSGALNACT1) 100057477(CHSY1) 100059511(CHPF) 100072984(CHSY3)
EPZ: 103540863(CHPF) 103541884 103542759 103548592(CHSY3) 103550620(CSGALNACT2) 103557115(CHSY1)
EAI: 106824857(CSGALNACT1) 106830921 106831340(CSGALNACT2) 106839479(CHSY1) 106840544(CHPF) 106842799(CHSY3)
MYB: 102240097(CHPF) 102240619(CHSY3) 102244364(CSGALNACT1) 102249440(CHSY1) 102262652(CSGALNACT2)
MYD: 102761493(CHPF) 102764495(CSGALNACT2) 102767110(CHSY3) 102768473(CHSY1) 102768920(CSGALNACT1)
HAI: 109375394(CHSY3) 109380347(CHSY1) 109380579(CHPF) 109386291(CSGALNACT1) 109395550(CSGALNACT2)
RSS: 109438924(CSGALNACT2) 109444955(CHSY1) 109452676(CHPF) 109452852(CSGALNACT1) 109460586(CHSY3)
PALE: 102878385(CSGALNACT2) 102881434(CHPF) 102889741(CSGALNACT1) 102890373(CHSY1) 102897068(CHSY3)
LAV: 100654033(CSGALNACT1) 100657082(CHSY1) 100661660(CSGALNACT2) 100671059(CHPF) 100677323(CHSY3)
MDO: 100010621(CHPF) 100012272(CSGALNACT2) 100020781(CHSY1) 100030310(CSGALNACT1)
SHR: 100913326(CHPF) 100923374(CHSY1) 100925396(CSGALNACT1) 100925905(CSGALNACT2)
OAA: 100076249(CSGALNACT2) 100078026(CSGALNACT1) 100680815 103170669(CHSY1)
GGA: 415521(CHSY1) 415598(CHSY3) 422488(CSGALNACT1) 423632(CSGALNACT2) 769112(CHPF)
MGP: 100538576(CSGALNACT1) 100540185(CHSY1) 100550036(CHPF) 100550089(CSGALNACT2)
CJO: 107306320(CHSY3) 107313214(CSGALNACT1) 107315593(CSGALNACT2) 107316900(CHPF) 107318958(CHSY1)
APLA: 101796158(CSGALNACT2) 101799131(CSGALNACT1) 101799372(CHSY3) 101800894(CHPF) 101801430(CHSY1)
ACYG: 106029815(CHPF) 106032482(CSGALNACT2) 106035122(CSGALNACT1) 106036484(CHSY1) 106045258(CHSY3)
TGU: 100222602(CHSY1) 100223510(CSGALNACT1) 100223757(CHSY3) 100225582(CHPF) 100231851(CSGALNACT2)
GFR: 102033481(CSGALNACT1) 102038477(CHPF) 102041114(CHSY3) 102043050(CSGALNACT2) 102043414(CHSY1)
FAB: 101809918(CHPF) 101812240(CSGALNACT1) 101812932(CHSY3) 101818662(CHSY1) 101820745(CSGALNACT2)
PHI: 102104336(CHSY1) 102105051(CHPF) 102107624(CHSY3) 102113363(CSGALNACT2) 102113419(CSGALNACT1)
PMAJ: 107203353(CSGALNACT1) 107207041(CSGALNACT2) 107207171(CHPF) 107209427(CHSY1) 107216421(CHSY3)
CCAE: 111922517(CHPF) 111931022(CSGALNACT2) 111933994(CHSY1) 111941742(CHSY3) 111944213(CSGALNACT1)
CCW: 104684076(CHSY1) 104689810(CHSY3) 104690220(CSGALNACT2) 104693941(CHPF) 104697139(CSGALNACT1)
FPG: 101910473(CSGALNACT1) 101919107(CSGALNACT2) 101919161(CHSY1) 101919353(CHSY3) 101923812(CHPF)
FCH: 102049509(CSGALNACT2) 102054731(CHSY1) 102054841(CHSY3) 102056391(CHPF) 102059429(CSGALNACT1)
CLV: 102088360(CHPF) 102088612(CSGALNACT1) 102090190(CHSY1) 102091017(CHSY3) 102097544(CSGALNACT2)
EGZ: 104122848(CHSY1) 104128815(CHPF) 104129088(CSGALNACT2) 104135239(CHSY3) 104135540(CSGALNACT1)
AAM: 106484360(CSGALNACT1) 106495060(CHSY3) 106495829(CHSY1) 106498950(CSGALNACT2) 106499326(CHPF)
ASN: 102375772(CHSY1) 102384714(CHSY3) 102388579(CSGALNACT2) 102388616(CSGALNACT1) 102388640(CHPF)
AMJ: 102562491(CHSY1) 102562660(CSGALNACT2) 102566190(CHSY3) 102572696(CHPF) 106736723(CSGALNACT1)
PSS: 102444940(CHSY3) 102446783(CHPF) 102447091(CSGALNACT1) 102458703(CSGALNACT2) 102459255(CHSY1)
CMY: 102932901(CHPF) 102938881(CHSY1) 102939048(CHSY3) 102940405(CSGALNACT2) 102945055(CSGALNACT1)
CPIC: 101937753(CSGALNACT2) 101942137(CHSY3) 101945190(CHSY1) 101949751(CHPF) 101950451(CSGALNACT1)
ACS: 100554248(chsy1) 100556433(chsy3) 100558813(csgalnact2) 100560221(chpf) 100560783(csgalnact1)
PVT: 110072945(CHSY1) 110078232(CHPF) 110081118(CHSY3) 110083670(CSGALNACT2) 110086324(CSGALNACT1)
PBI: 103048163(CSGALNACT1) 103049632(CHSY3) 103057166(CHSY1) 103058428(CSGALNACT2) 103068101(CHPF)
GJA: 107107034(CSGALNACT1) 107115005(CSGALNACT2) 107116102(CHSY1) 107119174 107123338(CHSY3) 107123996(CHPF)
XLA: 100158319(chpf.L) 108696289(csgalnact2.L) 108702913(chpf.S) 108706822(csgalnact1.S) 108707191 108711484(chsy1.L) 108712229(csgalnact1.L) 108712816(chsy1.S) 108717241
XTR: 100036707(chsy1) 100124741(csgalnact2) 100379780(chpf) 100494038(chsy3) 100497098(csgalnact1) 108645693
NPR: 108786604(CSGALNACT2) 108792306(CHPF) 108795493(CHSY1) 108797053 108804915(CSGALNACT1)
DRE: 100331155(chpfa) 324407(chsy1) 368477(chsy3) 560826(csgalnact2) 793671(csgalnact1a)
IPU: 108263569(csgalnact2) 108265223 108271046(chsy1) 108273176(chpf) 108277248(csgalnact1) 108277355(chsy3)
AMEX: 103021869 103029300(csgalnact2) 103038686(chsy3) 103040456 103042931(chpf) 103045746 103047541(chsy1)
TRU: 101061544(chpf) 101067591 101071007 101079035(chsy1) 101079052(csgalnact2) 101079135(csgalnact1)
LCO: 104920113(csgalnact1) 104920841 104921883(csgalnact2) 104927007(chpf) 104929727(chsy1) 104937434(chsy3) 104939177
MZE: 101469087(chpf) 101469922(csgalnact1) 101473522 101474654 101476345(csgalnact2) 101478644(chsy1)
OLA: 101155099(chpf) 101155793(csgalnact2) 101157180(chsy1) 101159591 101162617(csgalnact1)
XMA: 102221723(csgalnact1) 102232292(chsy1) 102233977 102237743(csgalnact2) 102237805(chpf)
PRET: 103460892(chpf) 103462273(chsy1) 103470100 103473476(csgalnact1) 103477114(csgalnact2)
NFU: 107372807(chsy1) 107373900(csgalnact1) 107375371(csgalnact2) 107390225(chpf) 107394138
KMR: 108231091(csgalnact1) 108233606 108238553(chpf) 108240679(csgalnact2) 108251564(chsy1)
CSEM: 103379026(chsy1) 103387459(csgalnact2) 103390413(csgalnact1) 103392304(chpf) 103397716
SDU: 111218469(chpf) 111220601(csgalnact2) 111222480(chsy1) 111230915 111239886(csgalnact1)
BPEC: 110156154(chpf) 110157439 110162232(csgalnact2) 110162594(chsy1) 110167048
MALB: 109956612 109960538(csgalnact2) 109972367 109972382(chpf) 109973812(chsy1)
ELS: 105014092 105014387(chsy1) 105024013(chsy3) 105026679(csgalnact1) 105028646(csgalnact2) 105029529(chpf)
SFM: 108924956(csgalnact1) 108927889 108928905 108929877(chsy3) 108932703(csgalnact2) 108940853(chsy1) 108941998
LCM: 102346351 102357727(CHSY3) 102358556(CSGALNACT2) 102363926(CHPF) 102366614(CSGALNACT1) 102366930(CHSY1)
CMK: 103172383 103178043(chsy3) 103181211(csgalnact2) 103186731(csgalnact1) 103187742(chsy1)
SPU: 579124 579341(CHSY1)
DME: Dmel_CG12913(CG12913) Dmel_CG43313(CG43313) Dmel_CG9220(CG9220)
DSI: Dsimw501_GD10724(Dsim_GD10724) Dsimw501_GD15794(Dsim_GD15794) Dsimw501_GD24523(Dsim_GD24523)
AME: 413549
FCD: 110850630
TUT: 107368932
CEL: CELE_PAR2.4(mig-22) CELE_T24D1.1(sqv-5)
CBR: CBG03828(Cbr-sqv-5) CBG06915(Cbr-mig-22)
PMU: PM0775
PMP: Pmu_08530(hyaD)
PMUL: DR93_1611(hyaD)
FBL: Fbal_0446
 » show all
Taxonomy
Reference
1  [PMID:3922754]
  Authors
Rohrmann K, Niemann R, Buddecke E.
  Title
Two N-acetylgalactosaminyltransferase are involved in the biosynthesis of chondroitin sulfate.
  Journal
Eur J Biochem 148:463-9 (1985)
DOI:10.1111/j.1432-1033.1985.tb08862.x
Reference
2  [PMID:11514575]
  Authors
Kitagawa H, Uyama T, Sugahara K.
  Title
Molecular cloning and expression of a human chondroitin synthase.
  Journal
J Biol Chem 276:38721-6 (2001)
DOI:10.1074/jbc.M106871200
  Sequence
[hsa:22856]
Reference
3  [PMID:10818104]
  Authors
DeAngelis PL, Padgett-McCue AJ.
  Title
Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F.
  Journal
J Biol Chem 275:24124-9 (2000)
DOI:10.1074/jbc.M003385200
  Sequence
[pmu:PM0775]
Reference
4  [PMID:11943778]
  Authors
Ninomiya T, Sugiura N, Tawada A, Sugimoto K, Watanabe H, Kimata K.
  Title
Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4.
  Journal
J Biol Chem 277:21567-75 (2002)
DOI:10.1074/jbc.M201719200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.175
IUBMB Enzyme Nomenclature: 2.4.1.175
ExPASy - ENZYME nomenclature database: 2.4.1.175
BRENDA, the Enzyme Database: 2.4.1.175
CAS: 96189-40-1

DBGET integrated database retrieval system