KEGG   ENZYME: 2.4.1.175Help
Entry
EC 2.4.1.175                Enzyme                                 

Name
glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase;
N-acetylgalactosaminyltransferase II;
UDP-N-acetyl-D-galactosamine:D-glucuronyl-N-acetyl-1,3-beta-D-galactosaminylproteoglycan beta-1,4-N-acetylgalactosaminyltransferase;
chondroitin synthase;
glucuronyl-N-acetylgalactosaminylproteoglycan beta-1,4-N-acetylgalactosaminyltransferase;
uridine diphosphoacetylgalactosamine-chondroitin acetylgalactosaminyltransferase II;
UDP-N-acetyl-D-galactosamine:beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase;
UDP-N-acetyl-alpha-D-galactosamine:beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-galactosamine:[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine 4-beta-N-acetylgalactosaminyltransferase (configuration-inverting)
Reaction(IUBMB)
(1) UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [RN:R04603 R05932];
(2) UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n+1-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Reaction(KEGG)
R04603 R05932(G);
(other) R05934(G)
Show
Substrate
UDP-N-acetyl-alpha-D-galactosamine [CPD:C00203];
[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [CPD:C04800];
[protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Product
UDP [CPD:C00015];
[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [CPD:C04913];
[protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n+1-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Comment
Involved in the biosynthesis of chondroitin sulfate. The human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase (EC 2.4.1.226, N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase) activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase 'co-polymerases' can be found in Pasteurella multocida and Escherichia coli.
History
EC 2.4.1.175 created 1989, modified 2002
Pathway
ec00532  Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
ec01100  Metabolic pathways
Orthology
K00746  chondroitin sulfate N-acetylgalactosaminyltransferase 1/2
K00747  chondroitin polymerizing factor
K13499  chondroitin sulfate synthase
K13500  chondroitin synthase
Genes
HSA: 22856(CHSY1) 337876(CHSY3) 55454(CSGALNACT2) 55790(CSGALNACT1) 79586(CHPF)
PTR: 450416(CSGALNACT2) 464030(CSGALNACT1) 467777(CHSY1) 471623(CHSY3) 743513(CHPF)
PPS: 100968775(CHSY1) 100971582(CHPF) 100972801(CHSY3) 100974901 100987599(CSGALNACT1) 100994377(CSGALNACT2)
GGO: 101126045(CHSY3) 101136357(CHSY1) 101144411(CHPF) 101151962(CSGALNACT2) 101154228(CSGALNACT1)
PON: 100172853(CSGALNACT2) 100442293(CSGALNACT1) 100449809(CHSY1) 103888550(CHPF)
NLE: 100583001(CSGALNACT1) 100588681(CHSY3) 100589763(CHPF) 100602432(CSGALNACT2) 100602507(CHSY1)
MCC: 694481(CHSY1) 703703(CSGALNACT2) 703860(CHPF) 704901(CSGALNACT1) 705715(CHSY3)
MCF: 101866114(CHPF) 102125913(CSGALNACT1) 102136125(CHSY1) 102136240(CHSY3) 102136560(CSGALNACT2)
CSAB: 103215414(CSGALNACT1) 103215756(CSGALNACT2) 103217919(CHPF) 103231288(CHSY1) 103244479(CHSY3)
RRO: 104653929(CSGALNACT2) 104660068(CHPF) 104662459(CSGALNACT1) 104676683(CHSY3) 104676811(CHSY1)
RBB: 108513317(CSGALNACT2) 108518435(CHPF) 108522937(CHSY1) 108526479(CSGALNACT1) 108531341(CHSY3)
CJC: 100392140(CHSY3) 100399122(CSGALNACT2) 100403329(CHPF) 100405485(CHSY1) 100407007(CSGALNACT1)
SBQ: 101027448(CSGALNACT2) 101046087(CHPF) 101047506(CHSY3) 101048885(CSGALNACT1) 101054458(CHSY1)
MMU: 234356(Csgalnact1) 269941(Chsy1) 74241(Chpf) 78752(Csgalnact2) 78923(Chsy3)
RNO: 100910780(Chsy3l) 291577(Chsy3) 292999(Chsy1) 297554(Csgalnact2) 306375(Csgalnact1) 316533(Chpf)
CGE: 100754969(Csgalnact1) 100765856(Chpf) 100767985(Chsy3) 100770803(Chsy1) 100770830(Csgalnact2)
NGI: 103727760(Chpf) 103733802(Csgalnact2) 103747203(Chsy1) 103747604(Csgalnact1) 103751045(Chsy3)
HGL: 101700301(Chsy1) 101700905(Csgalnact1) 101701741(Chsy3) 101707555(Chpf) 101719048(Csgalnact2)
CCAN: 109673993 109683396 109685049(Chsy1) 109688882(Csgalnact1) 109695115(Chpf) 109698852(Csgalnact2)
OCU: 100328892(CHPF) 100339411(CSGALNACT1) 100339435(CSGALNACT2) 100351896(CHSY3) 103351288(CHSY1)
TUP: 102468989(CSGALNACT2) 102488411(CHPF) 102490797(CSGALNACT1) 102491913(CHSY3) 102495682(CHSY1)
CFA: 481492(CHSY3) 482831(CSGALNACT1) 486786(CSGALNACT2) 488539(CHPF) 488704(CHSY1)
AML: 100466495(CHSY1) 100468746(CSGALNACT1) 100484507(CHSY3) 100484755(CSGALNACT2) 105240013(CHPF)
UMR: 103660037(CHSY1) 103662872(CHPF) 103667936(CSGALNACT2) 103670570(CHSY3) 103677036(CSGALNACT1)
ORO: 101369554(CSGALNACT2) 101377082(CSGALNACT1) 101380609(CHSY1) 101382953(CHSY3) 101383392(CHPF)
FCA: 101083179(CHSY1) 101096311(CSGALNACT1) 101097752(CHPF) 101100841(CSGALNACT2) 105260677 111556250(CHSY3)
PTG: 102952320(CHSY1) 102956824(CSGALNACT1) 102959394(CHPF) 102966347(CSGALNACT2) 102971026(CHSY3)
AJU: 106970041(CHPF) 106970692(CHSY3) 106972094(CHSY1) 106972130(CSGALNACT2) 106978257(CSGALNACT1)
BTA: 281690(CHSY1) 509328(CSGALNACT2) 528057(CSGALNACT1) 528149(CHSY3) 540002(CHPF)
BOM: 102266543(CSGALNACT1) 102281638(CHSY1) 102281650(CHSY3) 102284379(CHPF) 102285203(CSGALNACT2)
BIU: 109553356(CSGALNACT1) 109553754(CSGALNACT2) 109561832(CHSY3) 109571205(CHPF) 109575124(CHSY1)
PHD: 102319423(CHPF) 102326020 102327197 102330279(CSGALNACT2) 102333649(CSGALNACT1) 102334956 102344003(CHSY1)
CHX: 102170413(CHSY3) 102179324(CHSY1) 102179349(CSGALNACT1) 102181823(CHPF) 102185714(CSGALNACT2)
OAS: 101104485(CHSY3) 101109305(CHSY1) 101114088(CHPF) 101118243(CSGALNACT2) 101120469 105605252
SSC: 100157878(CSGALNACT2) 100517735 100518720(CHSY3) 100520288(CHSY1) 100623047(CHPF) 102166124(CSGALNACT1)
CFR: 102504589(CHSY3) 102513810 102514190(CSGALNACT2) 102514921(CSGALNACT1) 102523829(CHSY1) 102524541(CHPF)
CDK: 105086444(CSGALNACT1) 105089922(CHSY3) 105092509(CSGALNACT2) 105093456(CHSY1) 105096119(CHPF)
BACU: 103007226(CSGALNACT2) 103010484(CSGALNACT1) 103011106(CHPF) 103012595(CHSY1) 103019431(CHSY3)
LVE: 103069443(CSGALNACT2) 103069960(CHPF) 103072590(CHSY3) 103082442(CHSY1) 103083412 103084873(CSGALNACT1)
OOR: 101279931(CHPF) 101283608(CHSY3) 101287224(CHSY1) 101288535(CSGALNACT2) 101289782(CSGALNACT1)
ECB: 100050200(CSGALNACT2) 100052192(CSGALNACT1) 100057477(CHSY1) 100059511(CHPF) 100072984(CHSY3)
EPZ: 103540863(CHPF) 103541884 103542759 103548592(CHSY3) 103550620(CSGALNACT2) 103557115(CHSY1)
EAI: 106824857(CSGALNACT1) 106830921 106831340(CSGALNACT2) 106839479(CHSY1) 106840544(CHPF) 106842799(CHSY3)
MYB: 102240097(CHPF) 102240619(CHSY3) 102244364(CSGALNACT1) 102249440(CHSY1) 102262652(CSGALNACT2)
MYD: 102761493(CHPF) 102764495(CSGALNACT2) 102767110(CHSY3) 102768473(CHSY1) 102768920(CSGALNACT1)
HAI: 109375394(CHSY3) 109380347(CHSY1) 109380579(CHPF) 109386291(CSGALNACT1) 109395550(CSGALNACT2)
RSS: 109438924(CSGALNACT2) 109444955(CHSY1) 109452676(CHPF) 109452852(CSGALNACT1) 109460586(CHSY3)
PALE: 102878385(CSGALNACT2) 102881434(CHPF) 102889741(CSGALNACT1) 102890373(CHSY1) 102897068(CHSY3)
LAV: 100654033(CSGALNACT1) 100657082(CHSY1) 100661660(CSGALNACT2) 100671059(CHPF) 100677323(CHSY3)
MDO: 100010621(CHPF) 100012272(CSGALNACT2) 100020781(CHSY1) 100030310(CSGALNACT1)
SHR: 100913326(CHPF) 100923374(CHSY1) 100925396(CSGALNACT1) 100925905(CSGALNACT2)
OAA: 100076249(CSGALNACT2) 100078026(CSGALNACT1) 100680815 103170669(CHSY1)
GGA: 415521(CHSY1) 415598(CHSY3) 422488(CSGALNACT1) 423632(CSGALNACT2) 769112(CHPF)
MGP: 100538576(CSGALNACT1) 100540185(CHSY1) 100550036(CHPF) 100550089(CSGALNACT2)
CJO: 107306320(CHSY3) 107313214(CSGALNACT1) 107315593(CSGALNACT2) 107316900(CHPF) 107318958(CHSY1)
APLA: 101796158(CSGALNACT2) 101799131(CSGALNACT1) 101799372(CHSY3) 101800894(CHPF) 101801430(CHSY1)
ACYG: 106029815(CHPF) 106032482(CSGALNACT2) 106035122(CSGALNACT1) 106036484(CHSY1) 106045258(CHSY3)
TGU: 100222602(CHSY1) 100223510(CSGALNACT1) 100223757(CHSY3) 100225582(CHPF) 100231851(CSGALNACT2)
GFR: 102033481(CSGALNACT1) 102038477(CHPF) 102041114(CHSY3) 102043050(CSGALNACT2) 102043414(CHSY1)
FAB: 101809918(CHPF) 101812240(CSGALNACT1) 101812932(CHSY3) 101818662(CHSY1) 101820745(CSGALNACT2)
PHI: 102104336(CHSY1) 102105051(CHPF) 102107624(CHSY3) 102113363(CSGALNACT2) 102113419(CSGALNACT1)
PMAJ: 107203353(CSGALNACT1) 107207041(CSGALNACT2) 107207171(CHPF) 107209427(CHSY1) 107216421(CHSY3)
CCAE: 111922517(CHPF) 111931022(CSGALNACT2) 111933994(CHSY1) 111941742(CHSY3) 111944213(CSGALNACT1)
CCW: 104684076(CHSY1) 104689810(CHSY3) 104690220(CSGALNACT2) 104693941(CHPF) 104697139(CSGALNACT1)
FPG: 101910473(CSGALNACT1) 101919107(CSGALNACT2) 101919161(CHSY1) 101919353(CHSY3) 101923812(CHPF)
FCH: 102049509(CSGALNACT2) 102054731(CHSY1) 102054841(CHSY3) 102056391(CHPF) 102059429(CSGALNACT1)
CLV: 102088360(CHPF) 102088612(CSGALNACT1) 102090190(CHSY1) 102091017(CHSY3) 102097544(CSGALNACT2)
EGZ: 104122848(CHSY1) 104128815(CHPF) 104129088(CSGALNACT2) 104135239(CHSY3) 104135540(CSGALNACT1)
AAM: 106484360(CSGALNACT1) 106495060(CHSY3) 106495829(CHSY1) 106498950(CSGALNACT2) 106499326(CHPF)
ASN: 102375772(CHSY1) 102384714(CHSY3) 102388579(CSGALNACT2) 102388616(CSGALNACT1) 102388640(CHPF)
AMJ: 102562491(CHSY1) 102562660(CSGALNACT2) 102566190(CHSY3) 102572696(CHPF) 106736723(CSGALNACT1)
PSS: 102444940(CHSY3) 102446783(CHPF) 102447091(CSGALNACT1) 102458703(CSGALNACT2) 102459255(CHSY1)
CMY: 102932901(CHPF) 102938881(CHSY1) 102939048(CHSY3) 102940405(CSGALNACT2) 102945055(CSGALNACT1)
CPIC: 101937753(CSGALNACT2) 101942137(CHSY3) 101945190(CHSY1) 101949751(CHPF) 101950451(CSGALNACT1)
ACS: 100554248(chsy1) 100556433(chsy3) 100558813(csgalnact2) 100560221(chpf) 100560783(csgalnact1)
PVT: 110072945(CHSY1) 110078232(CHPF) 110081118(CHSY3) 110083670(CSGALNACT2) 110086324(CSGALNACT1)
PBI: 103048163(CSGALNACT1) 103049632(CHSY3) 103057166(CHSY1) 103058428(CSGALNACT2) 103068101(CHPF)
GJA: 107107034(CSGALNACT1) 107115005(CSGALNACT2) 107116102(CHSY1) 107119174 107123338(CHSY3) 107123996(CHPF)
XLA: 100158319(chpf.L) 108696289(csgalnact2.L) 108702913(chpf.S) 108706822(csgalnact1.S) 108707191 108711484(chsy1.L) 108712229(csgalnact1.L) 108712816(chsy1.S) 108717241
XTR: 100036707(chsy1) 100124741(csgalnact2) 100379780(chpf) 100494038(chsy3) 100497098(csgalnact1) 108645693
NPR: 108786604(CSGALNACT2) 108792306(CHPF) 108795493(CHSY1) 108797053 108804915(CSGALNACT1)
DRE: 100331155(chpfa) 324407(chsy1) 368477(chsy3) 560826(csgalnact2) 793671(csgalnact1a)
IPU: 108263569(csgalnact2) 108265223 108271046(chsy1) 108273176(chpf) 108277248(csgalnact1) 108277355(chsy3)
AMEX: 103021869 103029300(csgalnact2) 103038686(chsy3) 103040456 103042931(chpf) 103045746 103047541(chsy1)
TRU: 101061544(chpf) 101067591 101071007 101079035(chsy1) 101079052(csgalnact2) 101079135(csgalnact1)
LCO: 104920113(csgalnact1) 104920841 104921883(csgalnact2) 104927007(chpf) 104929727(chsy1) 104937434(chsy3) 104939177
MZE: 101469087(chpf) 101469922(csgalnact1) 101473522 101474654 101476345(csgalnact2) 101478644(chsy1)
OLA: 101155099(chpf) 101155793(csgalnact2) 101157180(chsy1) 101159591 101162617(csgalnact1)
XMA: 102221723(csgalnact1) 102232292(chsy1) 102233977 102237743(csgalnact2) 102237805(chpf)
PRET: 103460892(chpf) 103462273(chsy1) 103470100 103473476(csgalnact1) 103477114(csgalnact2)
NFU: 107372807(chsy1) 107373900(csgalnact1) 107375371(csgalnact2) 107390225(chpf) 107394138
KMR: 108231091(csgalnact1) 108233606 108238553(chpf) 108240679(csgalnact2) 108251564(chsy1)
CSEM: 103379026(chsy1) 103387459(csgalnact2) 103390413(csgalnact1) 103392304(chpf) 103397716
SDU: 111218469(chpf) 111220601(csgalnact2) 111222480(chsy1) 111230915 111239886(csgalnact1)
BPEC: 110156154(chpf) 110157439 110162232(csgalnact2) 110162594(chsy1) 110167048
MALB: 109956612 109960538(csgalnact2) 109972367 109972382(chpf) 109973812(chsy1)
ELS: 105014092 105014387(chsy1) 105024013(chsy3) 105026679(csgalnact1) 105028646(csgalnact2) 105029529(chpf)
SFM: 108924956(csgalnact1) 108927889 108928905 108929877(chsy3) 108932703(csgalnact2) 108940853(chsy1) 108941998
LCM: 102346351 102357727(CHSY3) 102358556(CSGALNACT2) 102363926(CHPF) 102366614(CSGALNACT1) 102366930(CHSY1)
CMK: 103172383 103178043(chsy3) 103181211(csgalnact2) 103186731(csgalnact1) 103187742(chsy1)
SPU: 579124 579341(CHSY1)
DME: Dmel_CG12913(CG12913) Dmel_CG43313(CG43313) Dmel_CG9220(CG9220)
DPE: 6597818
DSI: Dsimw501_GD10724(Dsim_GD10724) Dsimw501_GD15794(Dsim_GD15794) Dsimw501_GD24523(Dsim_GD24523)
AME: 413549
FCD: 110850630
TUT: 107368932
CEL: CELE_PAR2.4(mig-22) CELE_T24D1.1(sqv-5)
CBR: CBG03828(Cbr-sqv-5) CBG06915(Cbr-mig-22)
PMU: PM0775
PMP: Pmu_08530(hyaD)
PMUL: DR93_1611(hyaD)
FBL: Fbal_0446
 » show all
Taxonomy
Reference
1  [PMID:3922754]
  Authors
Rohrmann K, Niemann R, Buddecke E.
  Title
Two N-acetylgalactosaminyltransferase are involved in the biosynthesis of chondroitin sulfate.
  Journal
Eur J Biochem 148:463-9 (1985)
DOI:10.1111/j.1432-1033.1985.tb08862.x
Reference
2  [PMID:11514575]
  Authors
Kitagawa H, Uyama T, Sugahara K.
  Title
Molecular cloning and expression of a human chondroitin synthase.
  Journal
J Biol Chem 276:38721-6 (2001)
DOI:10.1074/jbc.M106871200
  Sequence
[hsa:22856]
Reference
3  [PMID:10818104]
  Authors
DeAngelis PL, Padgett-McCue AJ.
  Title
Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F.
  Journal
J Biol Chem 275:24124-9 (2000)
DOI:10.1074/jbc.M003385200
  Sequence
[pmu:PM0775]
Reference
4  [PMID:11943778]
  Authors
Ninomiya T, Sugiura N, Tawada A, Sugimoto K, Watanabe H, Kimata K.
  Title
Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4.
  Journal
J Biol Chem 277:21567-75 (2002)
DOI:10.1074/jbc.M201719200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.175
IUBMB Enzyme Nomenclature: 2.4.1.175
ExPASy - ENZYME nomenclature database: 2.4.1.175
BRENDA, the Enzyme Database: 2.4.1.175
CAS: 96189-40-1

KEGG   ENZYME: 2.4.1.226Help
Entry
EC 2.4.1.226                Enzyme                                 

Name
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase;
chondroitin glucuronyltransferase II;
alpha-D-glucuronate:N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan 3-beta-glucuronosyltransferase;
UDP-alpha-D-glucuronate:N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan 3-beta-glucuronosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-glucuronate:[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine 3-beta-glucuronosyltransferase (configuration-inverting)
Reaction(IUBMB)
(1) UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [RN:R05931];
(2) UDP-alpha-D-glucuronate + [protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Reaction(KEGG)
R05931(G);
(other) R05933(G) R07336
Show
Substrate
UDP-alpha-D-glucuronate [CPD:C00167];
[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine;
[protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Product
UDP [CPD:C00015];
[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [CPD:C04800];
[protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Comment
Involved in the biosynthesis of chondroitin and dermatan sulfate. The human chondroitin synthetase is a bifunctional glycosyltransferase, which has the 3-beta-glucuronosyltransferase and 4-beta-N-acetylgalactosaminyltransferase (EC 2.4.1.175) activities required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase 'co-polymerases' can be found in Pasteurella multocida and Escherichia coli. There is also another human protein with apparently only the 3-beta-glucuronosyltransferase activity.
History
EC 2.4.1.226 created 2002, modified 2018
Pathway
ec00532  Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
ec01100  Metabolic pathways
Orthology
K00747  chondroitin polymerizing factor
K03419  chondroitin polymerizing factor 2
K13499  chondroitin sulfate synthase
K13500  chondroitin synthase
Genes
HSA: 22856(CHSY1) 337876(CHSY3) 54480(CHPF2) 79586(CHPF)
PTR: 467777(CHSY1) 471623(CHSY3) 743513(CHPF)
PPS: 100968775(CHSY1) 100971582(CHPF) 100972801(CHSY3) 100981399(CHPF2)
GGO: 101126045(CHSY3) 101136357(CHSY1) 101144411(CHPF)
PON: 100445095(CHPF2) 100449809(CHSY1) 103888550(CHPF)
NLE: 100588681(CHSY3) 100589763(CHPF) 100600610(CHPF2) 100602507(CHSY1)
MCC: 100689486(CHPF2) 694481(CHSY1) 703860(CHPF) 705715(CHSY3)
MCF: 101866114(CHPF) 102122122(CHPF2) 102136125(CHSY1) 102136240(CHSY3)
CSAB: 103217919(CHPF) 103227251(CHPF2) 103231288(CHSY1) 103244479(CHSY3)
RRO: 104660068(CHPF) 104675407(CHPF2) 104676683(CHSY3) 104676811(CHSY1)
RBB: 108518435(CHPF) 108522937(CHSY1) 108531341(CHSY3) 108532540(CHPF2)
CJC: 100392140(CHSY3) 100403329(CHPF) 100405384(CHPF2) 100405485(CHSY1)
SBQ: 101041738(CHPF2) 101046087(CHPF) 101047506(CHSY3) 101054458(CHSY1)
MMU: 100910(Chpf2) 269941(Chsy1) 74241(Chpf) 78923(Chsy3)
RNO: 100910780(Chsy3l) 291577(Chsy3) 292999(Chsy1) 296733(Chpf2) 316533(Chpf)
CGE: 100760284 100765856(Chpf) 100767985(Chsy3) 100770803(Chsy1)
NGI: 103727760(Chpf) 103745316(Chpf2) 103747203(Chsy1) 103751045(Chsy3)
HGL: 101700301(Chsy1) 101701741(Chsy3) 101703677(Chpf2) 101707555(Chpf)
CCAN: 109673993 109683396 109685049(Chsy1) 109690748(Chpf2) 109695115(Chpf)
OCU: 100328892(CHPF) 100344738(CHPF2) 100351896(CHSY3) 103351288(CHSY1)
TUP: 102488411(CHPF) 102491913(CHSY3) 102495682(CHSY1)
CFA: 481492(CHSY3) 488539(CHPF) 488704(CHSY1)
AML: 100466495(CHSY1) 100481231(CHPF2) 100484507(CHSY3) 105240013(CHPF)
UMR: 103660037(CHSY1) 103660990(CHPF2) 103662872(CHPF) 103670570(CHSY3)
ORO: 101367866 101380609(CHSY1) 101382953(CHSY3) 101383392(CHPF) 101386610(CHPF2)
FCA: 101083179(CHSY1) 101097752(CHPF) 101099111(CHPF2) 105260677 111556250(CHSY3)
PTG: 102952320(CHSY1) 102959394(CHPF) 102969582(CHPF2) 102971026(CHSY3)
AJU: 106969568(CHPF2) 106970041(CHPF) 106970692(CHSY3) 106972094(CHSY1)
BTA: 100616099(CHPF2) 281690(CHSY1) 528149(CHSY3) 540002(CHPF)
BOM: 102264916(CHPF2) 102281638(CHSY1) 102281650(CHSY3) 102284379(CHPF)
BIU: 109557805(CHPF2) 109561832(CHSY3) 109571205(CHPF) 109575124(CHSY1)
CHX: 102170413(CHSY3) 102179324(CHSY1) 102181823(CHPF)
OAS: 101104485(CHSY3) 101109305(CHSY1) 101114088(CHPF) 101114606(CHPF2)
SSC: 100518720(CHSY3) 100520288(CHSY1) 100623047(CHPF)
CFR: 102504589(CHSY3) 102505571(CHPF2) 102523829(CHSY1) 102524541(CHPF)
CDK: 105089922(CHSY3) 105093456(CHSY1) 105096119(CHPF) 105098683(CHPF2)
BACU: 102999558(CHPF2) 103011106(CHPF) 103012595(CHSY1) 103019431(CHSY3)
LVE: 103069960(CHPF) 103072590(CHSY3) 103081746(CHPF2) 103082442(CHSY1) 103083412
OOR: 101279931(CHPF) 101283608(CHSY3) 101284703(CHPF2) 101287224(CHSY1)
ECB: 100057477(CHSY1) 100059511(CHPF) 100072984(CHSY3)
EPZ: 103540863(CHPF) 103548592(CHSY3) 103557115(CHSY1) 103564172(CHPF2)
EAI: 106823347(CHPF2) 106839479(CHSY1) 106840544(CHPF) 106842799(CHSY3)
MYB: 102240097(CHPF) 102240619(CHSY3) 102249440(CHSY1) 102251364(CHPF2)
MYD: 102757536(CHPF2) 102761493(CHPF) 102767110(CHSY3) 102768473(CHSY1)
HAI: 109375394(CHSY3) 109380347(CHSY1) 109380579(CHPF) 109389660(CHPF2)
RSS: 109435167(CHPF2) 109444955(CHSY1) 109452676(CHPF) 109460586(CHSY3)
PALE: 102881434(CHPF) 102890106(CHPF2) 102890373(CHSY1) 102897068(CHSY3)
LAV: 100657082(CHSY1) 100671059(CHPF) 100673470(CHPF2) 100677323(CHSY3)
MDO: 100010621(CHPF) 100015571(CHPF2) 100020781(CHSY1)
SHR: 100913326(CHPF) 100922404 100923374(CHSY1) 111721141(CHPF2)
OAA: 100082626(CHPF2) 100680815 103170669(CHSY1)
GGA: 415521(CHSY1) 415598(CHSY3) 426039(CHPF2) 769112(CHPF)
MGP: 100540185(CHSY1) 100544997(CHPF2) 100550036(CHPF)
CJO: 107306320(CHSY3) 107308711(CHPF2) 107316900(CHPF) 107318958(CHSY1)
APLA: 101792856(CHPF2) 101799372(CHSY3) 101800894(CHPF) 101801430(CHSY1)
ACYG: 106029815(CHPF) 106036484(CHSY1) 106045258(CHSY3) 106049036
TGU: 100222602(CHSY1) 100223757(CHSY3) 100225582(CHPF) 100229326(CHPF2)
GFR: 102033641(CHPF2) 102038477(CHPF) 102041114(CHSY3) 102043414(CHSY1)
FAB: 101809918(CHPF) 101812023(CHPF2) 101812932(CHSY3) 101818662(CHSY1)
PHI: 102104336(CHSY1) 102105051(CHPF) 102107624(CHSY3) 102110734(CHPF2)
PMAJ: 107199845(CHPF2) 107207171(CHPF) 107209427(CHSY1) 107216421(CHSY3)
CCAE: 111922517(CHPF) 111933994(CHSY1) 111941742(CHSY3) 111942574(CHPF2)
CCW: 104684076(CHSY1) 104684255(CHPF2) 104689810(CHSY3) 104693941(CHPF)
FPG: 101911790(CHPF2) 101919161(CHSY1) 101919353(CHSY3) 101923812(CHPF)
FCH: 102054731(CHSY1) 102054841(CHSY3) 102055615(CHPF2) 102056391(CHPF)
CLV: 102085164 102088360(CHPF) 102090190(CHSY1) 102091017(CHSY3)
EGZ: 104122848(CHSY1) 104127865(CHPF2) 104128815(CHPF) 104135239(CHSY3)
AAM: 106484437(CHPF2) 106495060(CHSY3) 106495829(CHSY1) 106499326(CHPF)
ASN: 102375772(CHSY1) 102383905(CHPF2) 102384714(CHSY3) 102388640(CHPF)
AMJ: 102562491(CHSY1) 102566190(CHSY3) 102572696(CHPF) 102576552(CHPF2)
PSS: 102444940(CHSY3) 102446783(CHPF) 102449597 102459255(CHSY1) 102462055
CMY: 102932901(CHPF) 102938881(CHSY1) 102939048(CHSY3) 102939164(CHPF2)
CPIC: 101942137(CHSY3) 101945190(CHSY1) 101949751(CHPF) 101950003(CHPF2)
ACS: 100554248(chsy1) 100556433(chsy3) 100560221(chpf)
PVT: 110072945(CHSY1) 110078232(CHPF) 110081118(CHSY3) 110082572(CHPF2)
PBI: 103049632(CHSY3) 103053825(CHPF2) 103057166(CHSY1) 103068101(CHPF)
GJA: 107116102(CHSY1) 107119174 107123338(CHSY3) 107123460(CHPF2) 107123996(CHPF)
XLA: 100158319(chpf.L) 108695510(chpf2.S) 108702913(chpf.S) 108707191 108711484(chsy1.L) 108712816(chsy1.S) 108717241
XTR: 100036707(chsy1) 100216189(chpf2) 100379780(chpf) 100494038(chsy3) 108645693
DRE: 100000654(chpf2) 100331155(chpfa) 324407(chsy1) 368477(chsy3)
IPU: 108268162(chpf2) 108271046(chsy1) 108273176(chpf) 108277355(chsy3)
AMEX: 103021853(chpf2) 103038686(chsy3) 103040456 103042931(chpf) 103047541(chsy1)
TRU: 101061544(chpf) 101061867(chpf2) 101067591 101079035(chsy1)
LCO: 104927007(chpf) 104929602(chpf2) 104929727(chsy1) 104937434(chsy3) 104939177
MZE: 101469087(chpf) 101470813(chpf2) 101473522 101478644(chsy1)
OLA: 101155099(chpf) 101157180(chsy1) 101162559(chpf2)
XMA: 102223626(chpf2) 102232292(chsy1) 102237805(chpf)
PRET: 103456833(chpf2) 103460892(chpf) 103462273(chsy1)
NFU: 107372807(chsy1) 107382546(chpf2) 107390225(chpf)
KMR: 108230426(chpf2) 108238553(chpf) 108251564(chsy1)
CSEM: 103377411(chpf2) 103379026(chsy1) 103392304(chpf)
LCF: 108880339 108886899(chpf) 108894287(chsy1) 108895025(chpf2) 108900706
SDU: 111218469(chpf) 111222480(chsy1) 111237922(chpf2)
BPEC: 110156154(chpf) 110162594(chsy1) 110170036(chpf2)
MALB: 109971514(chpf2) 109972382(chpf) 109973812(chsy1)
ELS: 105014387(chsy1) 105019378(chpf2) 105024013(chsy3) 105029529(chpf)
LCM: 102346351 102348174(CHPF2) 102357727(CHSY3) 102363926(CHPF) 102366930(CHSY1)
SPU: 578984 579341(CHSY1)
DME: Dmel_CG43313(CG43313) Dmel_CG9220(CG9220)
DPE: 6597818
DSI: Dsimw501_GD15794(Dsim_GD15794) Dsimw501_GD24523(Dsim_GD24523)
TUT: 107368932
CEL: CELE_PAR2.4(mig-22) CELE_T24D1.1(sqv-5)
CBR: CBG03828(Cbr-sqv-5) CBG06915(Cbr-mig-22)
CRG: 105340352
EPA: 110236249
PMU: PM0775
PMP: Pmu_08530(hyaD)
PMUL: DR93_1611(hyaD)
FBL: Fbal_0446
 » show all
Taxonomy
Reference
1  [PMID:11514575]
  Authors
Kitagawa H, Uyama T, Sugahara K.
  Title
Molecular cloning and expression of a human chondroitin synthase.
  Journal
J Biol Chem 276:38721-6 (2001)
DOI:10.1074/jbc.M106871200
  Sequence
[hsa:22856]
Reference
2  [PMID:10818104]
  Authors
DeAngelis PL, Padgett-McCue AJ.
  Title
Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F.
  Journal
J Biol Chem 275:24124-9 (2000)
DOI:10.1074/jbc.M003385200
  Sequence
[pmu:PM0775]
Reference
3  [PMID:11943778]
  Authors
Ninomiya T, Sugiura N, Tawada A, Sugimoto K, Watanabe H, Kimata K.
  Title
Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4.
  Journal
J Biol Chem 277:21567-75 (2002)
DOI:10.1074/jbc.M201719200
  Sequence
Reference
4  [PMID:12145278]
  Authors
Gotoh M, Yada T, Sato T, Akashima T, Iwasaki H, Mochizuki H, Inaba N, Togayachi A, Kudo T, Watanabe H, Kimata K, Narimatsu H.
  Title
Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine.
  Journal
J Biol Chem 277:38179-88 (2002)
DOI:10.1074/jbc.M202601200
  Sequence
[hsa:54480]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.226
IUBMB Enzyme Nomenclature: 2.4.1.226
ExPASy - ENZYME nomenclature database: 2.4.1.226
BRENDA, the Enzyme Database: 2.4.1.226
CAS: 269077-98-7

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