KEGG   ENZYME: 2.4.1.226Help
Entry
EC 2.4.1.226                Enzyme                                 

Name
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase;
chondroitin glucuronyltransferase II;
alpha-D-glucuronate:N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan 3-beta-glucuronosyltransferase;
UDP-alpha-D-glucuronate:N-acetyl-beta-D-galactosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan 3-beta-glucuronosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-glucuronate:[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine 3-beta-glucuronosyltransferase (configuration-inverting)
Reaction(IUBMB)
(1) UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [RN:R05931];
(2) UDP-alpha-D-glucuronate + [protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Reaction(KEGG)
R05931(G);
(other) R05933(G) R07336
Show
Substrate
UDP-alpha-D-glucuronate [CPD:C00167];
[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine;
[protein]-3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Product
UDP [CPD:C00015];
[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine [CPD:C04800];
[protein]-3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)]n-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Comment
Involved in the biosynthesis of chondroitin and dermatan sulfate. The human chondroitin synthetase is a bifunctional glycosyltransferase, which has the 3-beta-glucuronosyltransferase and 4-beta-N-acetylgalactosaminyltransferase (EC 2.4.1.175) activities required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase 'co-polymerases' can be found in Pasteurella multocida and Escherichia coli. There is also another human protein with apparently only the 3-beta-glucuronosyltransferase activity.
History
EC 2.4.1.226 created 2002, modified 2018
Pathway
ec00532  Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate
ec01100  Metabolic pathways
Orthology
K00747  chondroitin polymerizing factor
K03419  chondroitin polymerizing factor 2
K13499  chondroitin sulfate synthase
K13500  chondroitin synthase
Genes
HSA: 22856(CHSY1) 337876(CHSY3) 54480(CHPF2) 79586(CHPF)
PTR: 467777(CHSY1) 471623(CHSY3) 743513(CHPF)
PPS: 100968775(CHSY1) 100971582(CHPF) 100972801(CHSY3) 100981399(CHPF2)
GGO: 101126045(CHSY3) 101136357(CHSY1) 101144411(CHPF)
PON: 100445095(CHPF2) 100449809(CHSY1) 103888550(CHPF)
NLE: 100588681(CHSY3) 100589763(CHPF) 100600610(CHPF2) 100602507(CHSY1)
MCC: 100689486(CHPF2) 694481(CHSY1) 703860(CHPF) 705715(CHSY3)
MCF: 101866114(CHPF) 102122122(CHPF2) 102136125(CHSY1) 102136240(CHSY3)
CSAB: 103217919(CHPF) 103227251(CHPF2) 103231288(CHSY1) 103244479(CHSY3)
RRO: 104660068(CHPF) 104675407(CHPF2) 104676683(CHSY3) 104676811(CHSY1)
RBB: 108518435(CHPF) 108522937(CHSY1) 108531341(CHSY3) 108532540(CHPF2)
CJC: 100392140(CHSY3) 100403329(CHPF) 100405384(CHPF2) 100405485(CHSY1)
SBQ: 101041738(CHPF2) 101046087(CHPF) 101047506(CHSY3) 101054458(CHSY1)
MMU: 100910(Chpf2) 269941(Chsy1) 74241(Chpf) 78923(Chsy3)
RNO: 100910780(Chsy3l) 291577(Chsy3) 292999(Chsy1) 296733(Chpf2) 316533(Chpf)
CGE: 100760284 100765856(Chpf) 100767985(Chsy3) 100770803(Chsy1)
NGI: 103727760(Chpf) 103745316(Chpf2) 103747203(Chsy1) 103751045(Chsy3)
HGL: 101700301(Chsy1) 101701741(Chsy3) 101703677(Chpf2) 101707555(Chpf)
CCAN: 109673993 109683396 109685049(Chsy1) 109690748(Chpf2) 109695115(Chpf)
OCU: 100328892(CHPF) 100344738(CHPF2) 100351896(CHSY3) 103351288(CHSY1)
TUP: 102488411(CHPF) 102491913(CHSY3) 102495682(CHSY1)
CFA: 481492(CHSY3) 488539(CHPF) 488704(CHSY1)
AML: 100466495(CHSY1) 100481231(CHPF2) 100484507(CHSY3) 105240013(CHPF)
UMR: 103660037(CHSY1) 103660990(CHPF2) 103662872(CHPF) 103670570(CHSY3)
ORO: 101367866 101380609(CHSY1) 101382953(CHSY3) 101383392(CHPF) 101386610(CHPF2)
FCA: 101083179(CHSY1) 101097752(CHPF) 101099111(CHPF2) 105260677 111556250(CHSY3)
PTG: 102952320(CHSY1) 102959394(CHPF) 102969582(CHPF2) 102971026(CHSY3)
AJU: 106969568(CHPF2) 106970041(CHPF) 106970692(CHSY3) 106972094(CHSY1)
BTA: 100616099(CHPF2) 281690(CHSY1) 528149(CHSY3) 540002(CHPF)
BOM: 102264916(CHPF2) 102281638(CHSY1) 102281650(CHSY3) 102284379(CHPF)
BIU: 109557805(CHPF2) 109561832(CHSY3) 109571205(CHPF) 109575124(CHSY1)
CHX: 102170413(CHSY3) 102179324(CHSY1) 102181823(CHPF)
OAS: 101104485(CHSY3) 101109305(CHSY1) 101114088(CHPF) 101114606(CHPF2)
SSC: 100518720(CHSY3) 100520288(CHSY1) 100623047(CHPF)
CFR: 102504589(CHSY3) 102505571(CHPF2) 102523829(CHSY1) 102524541(CHPF)
CDK: 105089922(CHSY3) 105093456(CHSY1) 105096119(CHPF) 105098683(CHPF2)
BACU: 102999558(CHPF2) 103011106(CHPF) 103012595(CHSY1) 103019431(CHSY3)
LVE: 103069960(CHPF) 103072590(CHSY3) 103081746(CHPF2) 103082442(CHSY1) 103083412
OOR: 101279931(CHPF) 101283608(CHSY3) 101284703(CHPF2) 101287224(CHSY1)
ECB: 100057477(CHSY1) 100059511(CHPF) 100072984(CHSY3)
EPZ: 103540863(CHPF) 103548592(CHSY3) 103557115(CHSY1) 103564172(CHPF2)
EAI: 106823347(CHPF2) 106839479(CHSY1) 106840544(CHPF) 106842799(CHSY3)
MYB: 102240097(CHPF) 102240619(CHSY3) 102249440(CHSY1) 102251364(CHPF2)
MYD: 102757536(CHPF2) 102761493(CHPF) 102767110(CHSY3) 102768473(CHSY1)
HAI: 109375394(CHSY3) 109380347(CHSY1) 109380579(CHPF) 109389660(CHPF2)
RSS: 109435167(CHPF2) 109444955(CHSY1) 109452676(CHPF) 109460586(CHSY3)
PALE: 102881434(CHPF) 102890106(CHPF2) 102890373(CHSY1) 102897068(CHSY3)
LAV: 100657082(CHSY1) 100671059(CHPF) 100673470(CHPF2) 100677323(CHSY3)
MDO: 100010621(CHPF) 100015571(CHPF2) 100020781(CHSY1)
SHR: 100913326(CHPF) 100922404 100923374(CHSY1) 111721141(CHPF2)
OAA: 100082626(CHPF2) 100680815 103170669(CHSY1)
GGA: 415521(CHSY1) 415598(CHSY3) 426039(CHPF2) 769112(CHPF)
MGP: 100540185(CHSY1) 100544997(CHPF2) 100550036(CHPF)
CJO: 107306320(CHSY3) 107308711(CHPF2) 107316900(CHPF) 107318958(CHSY1)
APLA: 101792856(CHPF2) 101799372(CHSY3) 101800894(CHPF) 101801430(CHSY1)
ACYG: 106029815(CHPF) 106036484(CHSY1) 106045258(CHSY3) 106049036
TGU: 100222602(CHSY1) 100223757(CHSY3) 100225582(CHPF) 100229326(CHPF2)
GFR: 102033641(CHPF2) 102038477(CHPF) 102041114(CHSY3) 102043414(CHSY1)
FAB: 101809918(CHPF) 101812023(CHPF2) 101812932(CHSY3) 101818662(CHSY1)
PHI: 102104336(CHSY1) 102105051(CHPF) 102107624(CHSY3) 102110734(CHPF2)
PMAJ: 107199845(CHPF2) 107207171(CHPF) 107209427(CHSY1) 107216421(CHSY3)
CCW: 104684076(CHSY1) 104684255(CHPF2) 104689810(CHSY3) 104693941(CHPF)
FPG: 101911790(CHPF2) 101919161(CHSY1) 101919353(CHSY3) 101923812(CHPF)
FCH: 102054731(CHSY1) 102054841(CHSY3) 102055615(CHPF2) 102056391(CHPF)
CLV: 102085164 102088360(CHPF) 102090190(CHSY1) 102091017(CHSY3)
EGZ: 104122848(CHSY1) 104127865(CHPF2) 104128815(CHPF) 104135239(CHSY3)
AAM: 106484437(CHPF2) 106495060(CHSY3) 106495829(CHSY1) 106499326(CHPF)
ASN: 102375772(CHSY1) 102383905(CHPF2) 102384714(CHSY3) 102388640(CHPF)
AMJ: 102562491(CHSY1) 102566190(CHSY3) 102572696(CHPF) 102576552(CHPF2)
PSS: 102444940(CHSY3) 102446783(CHPF) 102449597 102459255(CHSY1) 102462055
CMY: 102932901(CHPF) 102938881(CHSY1) 102939048(CHSY3) 102939164(CHPF2)
CPIC: 101942137(CHSY3) 101945190(CHSY1) 101949751(CHPF) 101950003(CHPF2)
ACS: 100554248(chsy1) 100556433(chsy3) 100560221(chpf)
PVT: 110072945(CHSY1) 110078232(CHPF) 110081118(CHSY3) 110082572(CHPF2)
PBI: 103049632(CHSY3) 103053825(CHPF2) 103057166(CHSY1) 103068101(CHPF)
GJA: 107116102(CHSY1) 107119174 107123338(CHSY3) 107123460(CHPF2) 107123996(CHPF)
XLA: 100158319(chpf.L) 108695510(chpf2.S) 108702913(chpf.S) 108707191 108711484(chsy1.L) 108712816(chsy1.S) 108717241
XTR: 100036707(chsy1) 100216189(chpf2) 100379780(chpf) 100494038(chsy3) 108645693
DRE: 100000654(chpf2) 100331155(chpfa) 324407(chsy1) 368477(chsy3)
IPU: 108268162(chpf2) 108271046(chsy1) 108273176(chpf) 108277355(chsy3)
AMEX: 103021853(chpf2) 103038686(chsy3) 103040456 103042931(chpf) 103047541(chsy1)
TRU: 101061544(chpf) 101061867(chpf2) 101067591 101079035(chsy1)
LCO: 104927007(chpf) 104929602(chpf2) 104929727(chsy1) 104937434(chsy3) 104939177
MZE: 101469087(chpf) 101470813(chpf2) 101473522 101478644(chsy1)
OLA: 101155099(chpf) 101157180(chsy1) 101162559(chpf2)
XMA: 102223626(chpf2) 102232292(chsy1) 102237805(chpf)
PRET: 103456833(chpf2) 103460892(chpf) 103462273(chsy1)
NFU: 107372807(chsy1) 107382546(chpf2) 107390225(chpf)
CSEM: 103377411(chpf2) 103379026(chsy1) 103392304(chpf)
LCF: 108880339 108886899(chpf) 108894287(chsy1) 108895025(chpf2) 108900706
BPEC: 110156154(chpf) 110162594(chsy1) 110170036(chpf2)
ELS: 105014387(chsy1) 105019378(chpf2) 105024013(chsy3) 105029529(chpf)
LCM: 102346351 102348174(CHPF2) 102357727(CHSY3) 102363926(CHPF) 102366930(CHSY1)
SPU: 578984 579341(CHSY1)
DME: Dmel_CG43313(CG43313) Dmel_CG9220(CG9220)
DSI: Dsimw501_GD15794(Dsim_GD15794) Dsimw501_GD24523(Dsim_GD24523)
TUT: 107368932
CEL: CELE_PAR2.4(mig-22) CELE_T24D1.1(sqv-5)
CBR: CBG03828(Cbr-sqv-5) CBG06915(Cbr-mig-22)
CRG: 105340352
EPA: 110236249
PMU: PM0775
PMP: Pmu_08530(hyaD)
PMUL: DR93_1611(hyaD)
FBL: Fbal_0446
 » show all
Taxonomy
Reference
1  [PMID:11514575]
  Authors
Kitagawa H, Uyama T, Sugahara K.
  Title
Molecular cloning and expression of a human chondroitin synthase.
  Journal
J Biol Chem 276:38721-6 (2001)
DOI:10.1074/jbc.M106871200
  Sequence
[hsa:22856]
Reference
2  [PMID:10818104]
  Authors
DeAngelis PL, Padgett-McCue AJ.
  Title
Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F.
  Journal
J Biol Chem 275:24124-9 (2000)
DOI:10.1074/jbc.M003385200
  Sequence
[pmu:PM0775]
Reference
3  [PMID:11943778]
  Authors
Ninomiya T, Sugiura N, Tawada A, Sugimoto K, Watanabe H, Kimata K.
  Title
Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4.
  Journal
J Biol Chem 277:21567-75 (2002)
DOI:10.1074/jbc.M201719200
  Sequence
Reference
4  [PMID:12145278]
  Authors
Gotoh M, Yada T, Sato T, Akashima T, Iwasaki H, Mochizuki H, Inaba N, Togayachi A, Kudo T, Watanabe H, Kimata K, Narimatsu H.
  Title
Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine.
  Journal
J Biol Chem 277:38179-88 (2002)
DOI:10.1074/jbc.M202601200
  Sequence
[hsa:54480]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.226
IUBMB Enzyme Nomenclature: 2.4.1.226
ExPASy - ENZYME nomenclature database: 2.4.1.226
BRENDA, the Enzyme Database: 2.4.1.226
CAS: 269077-98-7

DBGET integrated database retrieval system