KEGG   ENZYME: 2.4.1.229Help
Entry
EC 2.4.1.229                Enzyme                                 

Name
[Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase;
Skp1-HyPro GlcNAc-transferase;
UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-transferase;
UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase;
UDP-GlcNAc:hydroxyproline polypeptide GlcNAc-transferase;
UDP-N-acetyl-D-glucosamine:[Skp1-protein]-hydroxyproline N-acetyl-D-glucosaminyl-transferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:[Skp1-protein]-trans-4-hydroxy-L-proline N-acetyl-alpha-D-glucosaminyl-transferase
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + [Skp1-protein]-trans-4-hydroxy-L-proline = UDP + [Skp1-protein]-O-(N-acetyl-alpha-D-glucosaminyl)-trans-4-hydroxy-L-proline
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
[Skp1-protein]-trans-4-hydroxy-L-proline
Product
UDP [CPD:C00015];
[Skp1-protein]-O-(N-acetyl-alpha-D-glucosaminyl)-trans-4-hydroxy-L-proline
Comment
Skp1 is a cytoplasmic and nuclear protein required for the ubiquitination of cell cycle regulatory proteins and transcriptional factors. In Dictyostelium Skp1 is modified by the linear pentasaccharide Galalpha1-6Galalpha1-L-Fucalpha1-2Galbeta1-3GlcNAc, which is attached to a hydroxyproline residue at position 143. This enzyme catalyses the first step in the building up of the pentasaccharide by attaching an N-acetylglucosaminyl group to the hydroxyproline residue. It requires dithiothreitol and a divalent cation for activity.
History
EC 2.4.1.229 created 2003, modified 2013
Orthology
K12244  [Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase
Genes
VCN: VOLCADRAFT_96541
CSL: COCSUDRAFT_58780 COCSUDRAFT_60908
DDI: DDB_G0275699(gnt1) DDB_G0291241(gnt2)
DPP: DICPUDRAFT_89733(gnt1)
DFA: DFA_01363(gnt1) DFA_01972(gnt2)
ACAN: ACA1_174930
TPS: THAPSDRAFT_261550 THAPSDRAFT_4441
NGD: NGA_2029800
SPAR: SPRG_09935
EHX: EMIHUDRAFT_453190
 » show all
Taxonomy
Reference
1  [PMID:12244115]
  Authors
Van Der Wel H, Morris HR, Panico M, Paxton T, Dell A, Kaplan L, West CM.
  Title
Molecular cloning and expression of a UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-transferase that modifies Skp1 in the cytoplasm of dictyostelium.
  Journal
J Biol Chem 277:46328-37 (2002)
DOI:10.1074/jbc.M208024200
  Sequence
Reference
2  [PMID:10593934]
  Authors
Teng-Umnuay P, van der Wel H, West CM.
  Title
Identification of a UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase in the cytoplasm of Dictyostelium.
  Journal
J Biol Chem 274:36392-402 (1999)
DOI:10.1074/jbc.274.51.36392
  Sequence
Reference
3  [PMID:11886837]
  Authors
West CM, van der Wel H, Gaucher EA.
  Title
Complex glycosylation of Skp1 in Dictyostelium: implications for the modification of other eukaryotic cytoplasmic and nuclear proteins.
  Journal
Glycobiology 12:17R-27R (2002)
DOI:10.1093/glycob/12.2.17R
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.229
IUBMB Enzyme Nomenclature: 2.4.1.229
ExPASy - ENZYME nomenclature database: 2.4.1.229
BRENDA, the Enzyme Database: 2.4.1.229
CAS: 256531-81-4

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