KEGG   ENZYME: 2.4.1.250Help
Entry
EC 2.4.1.250                Enzyme                                 

Name
D-inositol-3-phosphate glycosyltransferase;
mycothiol glycosyltransferases;
MshA;
UDP-N-acetyl-D-glucosamine:1D-myo-inositol 3-phosphate alpha-D-glycosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-glucosamine:1D-myo-inositol 3-phosphate alpha-D-glycosyltransferase (configuration-retaining)
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-glucosamine + 1D-myo-inositol 3-phosphate = 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate + UDP [RN:R09634]
Reaction(KEGG)
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
1D-myo-inositol 3-phosphate [CPD:C04006]
Product
1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate [CPD:C19761];
UDP [CPD:C00015]
Comment
The enzyme, which belongs to the GT-B fold superfamily, catalyses the first dedicated reaction in the biosynthesis of mycothiol [1]. The substrate was initially believed to be inositol, but eventually shown to be D-myo-inositol 3-phosphate [2]. A substantial conformational change occurs upon UDP binding, which generates the binding site for D-myo-inositol 3-phosphate [3].
History
EC 2.4.1.250 created 2010
Orthology
K15521  D-inositol-3-phosphate glycosyltransferase
Genes
MASS: CR152_19275
SSY: SLG_37050
PKU: AUO94_14520
PRT: AUC31_07605
PANA: BBH88_00320
PDG: BCM40_13660
PHC: BBI08_15060
PPLA: BBI15_14415
PFAE: AJGP001_02580
PLX: CW734_03760
MTU: Rv0486(mshA)
MTV: RVBD_0486
MTC: MT0504
MRA: MRA_0493
MTUR: CFBS_0506
MTD: UDA_0486
MTUB: MT7199_0496(mshA)
MTUE: J114_02585
MTUH: I917_03485
MTUL: TBHG_00484
MTUT: HKBT1_0506
MTUU: HKBT2_0506
MBO: BQ2027_MB0496(msha)
MBB: BCG_0527
MBT: JTY_0497
MBX: BCGT_0261
MAF: MAF_04900
MMIC: RN08_0540
MCQ: BN44_10539(mshA)
MCV: BN43_10528(mshA)
MCX: BN42_20213(mshA)
MCZ: BN45_10536(mshA)
MLE: ML2443
MLB: MLBr02443
MPA: MAP_3979
MAO: MAP4_4095
MAVI: RC58_20345
MAV: MAV_4664
MAVD: NF84_21510
MAVR: LA63_21725
MAVA: LA64_21690
MIT: OCO_45560
MIA: OCU_45280
MID: MIP_06904
MYO: OEM_45700
MIR: OCQ_46610
MMAL: CKJ54_21665(mshA)
MLP: MLM_3829
MUL: MUL_4556
MMC: Mmcs_0657
MKM: Mkms_0670
MJL: Mjls_0650
MMI: MMAR_0812
MMM: W7S_22905
MHAD: B586_19590
MVA: Mvan_0827
MGI: Mflv_0086
MFT: XA26_52200(mshA)
MPHL: MPHLCCUG_04512(mshA_2)
MVQ: MYVA_0658
MTHN: 4412656_00583(mshA_1)
MAB: MAB_4057c
MABB: MASS_4068
MABO: NF82_20335
MCHE: BB28_20905
MSTE: MSTE_04227(mshA)
MTER: 4434518_00470(mshA_1)
ASD: AS9A_0623(mshA)
CGL: NCgl0389(Cgl0401)
CGB: cg0481
CGU: WA5_0389(MshA)
CGT: cgR_0473
CGM: cgp_0481(mshA)
CGJ: AR0_02555
CEF: CE0422
CDI: DIP0388
CDP: CD241_0326(mshA)
CDH: CDB402_0302(mshA)
CDT: CDHC01_0327(mshA)
CDE: CDHC02_0338(mshA)
CDR: CDHC03_0317(mshA)
CDA: CDHC04_0298(mshA)
CDZ: CD31A_0389(mshA)
CDB: CDBH8_0327(mshA)
CDS: CDC7B_0332(mshA)
CDD: CDCE8392_0338(mshA)
CDW: CDPW8_0388(mshA)
CDV: CDVA01_0281(mshA)
CDIP: ERS451417_00310(mshA)
CJK: jk1914
CUR: cu0213
CUA: CU7111_0218(mshA)
CAR: cauri_0284(mshA)
CPU: cpfrc_00256(mshA)
CPL: Cp3995_0259(mshA)
CPP: CpP54B96_0261(mshA)
CPK: Cp1002_0257(mshA)
CPQ: CpC231_0260(mshA)
CPX: CpI19_0259(mshA)
CPZ: CpPAT10_0262(mshA)
COR: Cp267_0271(mshA)
COP: Cp31_0265(mshA)
COD: Cp106_0250(mshA)
COS: Cp4202_0254(mshA)
COI: CpCIP5297_0263(mshA)
COE: Cp258_0261(mshA)
COU: Cp162_0254(mshA)
CPSE: CPTA_00788
CPSU: CPTB_00744
CPSF: CPTC_00406
CRD: CRES_1964(mshA)
CUL: CULC22_00304(mshA)
CUC: CULC809_00300(mshA)
CUE: CULC0102_0350(mshA)
CUN: Cul210932_0316(mshA)
CUS: CulFRC11_0299(mshA)
CUQ: Cul210931_0304(mshA)
CUZ: Cul05146_0325(mshA)
CUJ: CUL131002_0302(mshA)
CVA: CVAR_2751(mshA)
CTER: A606_10195
CGY: CGLY_03145(mshA)
COA: DR71_1349(mshA)
CSX: CSING_01650(mshA)
CMQ: B840_01475(mshA)
CKU: UL82_01190(mshA)
CCJ: UL81_01260(mshA)
CMV: CMUST_01540(mshA)
CEI: CEPID_11160(mshA)
CTED: CTEST_01540(mshA)
CUT: CUTER_01180(mshA)
CDX: CDES_02215(mshA)
CSP: WM42_1075(mshA)
CPHO: CPHO_01585
CGV: CGLAU_01270(mshA)
CAMG: CAMM_01955
CMIN: NCTC10288_02245(mshA)
NFA: NFA_51920(mshA)
NFR: ERS450000_04691(mshA_3)
NNO: NONO_c70620(mshA)
NSR: NS506_00164(mshA)
NTP: CRH09_37055(mshA)
RER: RER_16170(mshA)
REY: O5Y_07720
ROP: ROP_17450(mshA)
REQ: REQ_37850(mshA)
RHB: NY08_4710
RFA: A3L23_02775(mshA_3)
RHS: A3Q41_00585(mshA_1)
RRZ: CS378_08885(mshA)
RHU: A3Q40_00239(mshA_1)
RQI: C1M55_08190(mshA)
RRT: 4535765_03708(mshA_1)
GBR: Gbro_0989
GPO: GPOL_c10210(mshA)
GOR: KTR9_0891
GOC: CXX93_00240(mshA)
GIT: C6V83_17130(mshA)
TPR: Tpau_0627
SRT: Srot_2070
DIT: C3V38_03185(mshA)
DIZ: CT688_13725(mshA)
SCO: SCO4204(2SCD46.18)
SMA: SAVERM_4006(mshA)
SGR: SGR_3993
SGB: WQO_15655
SCT: SCAT_2500
SFA: Sfla_2850
SBH: SBI_05009
SHY: SHJG_4888
SDV: BN159_4831(mshA)
SALB: XNR_3380
SALS: SLNWT_4108
STRP: F750_3943(mshA)
SFI: SFUL_4029
SALU: DC74_4731
SALL: SAZ_25230
SLV: SLIV_17355(mshA)
SGU: SGLAU_15730(mshA)
STRE: GZL_04111
SLD: T261_3494
STRM: M444_16420
SAMB: SAM23877_3492(mshA)
SPRI: SPRI_4052
SRW: TUE45_04740(mshA_4)
SLE: sle_38490(sle_38490)
SRN: A4G23_02492(mshA_4)
SNR: SNOUR_18260(mshA)
STRD: NI25_21375
SMAL: SMALA_3526
SALJ: SMD11_2898
SLX: SLAV_09105(mshA1) SLAV_17730(mshA2)
SFK: KY5_3749c
KSK: KSE_34590
STRI: C7M71_013285(mshA)
TWH: TWT_797
TWS: TW807
CMI: CMM_2955
CMS: CMS3091
CMC: CMN_02927
ART: Arth_2900
ARM: ART_2068
ARN: CGK93_16520(mshA)
ARX: ARZXY2_789(mshA)
ARTH: C3B78_13905(mshA)
AAU: AAur_2891
ACH: Achl_2609
KRH: KRH_06620(mshA)
JDE: Jden_2087
LMOI: VV02_07265
XCE: Xcel_2973
IDO: I598_3033(mshA_1)
CFL: Cfla_0653
CFI: Celf_0584
SERJ: SGUI_2603
ORN: DV701_08505(mshA)
BLIN: BLSMQ_3298
DCO: SAMEA4475696_0882(mshA)
PFRE: RM25_2108
MPH: MLP_42560
NCA: Noca_4050
NDK: I601_1838(mshA_1)
KFL: Kfla_1007
PSIM: KR76_22105
AEZ: C3E78_02775(mshA)
MGG: MPLG2_3560(mshA)
TFU: Tfu_2913
NDA: Ndas_4336
NAL: B005_1520(mshA)
NGV: CDO52_04710(mshA)
TCU: Tcur_0695
SRO: Sros_0956
FAL: FRAAL0950
ACE: Acel_0073
NML: Namu_4935
GOB: Gobs_4609
MMAR: MODMU_5019
KRA: Krad_0890
SEN: SACE_6971
AMD: AMED_0453
AMN: RAM_02330
AMM: AMES_0453
AMZ: B737_0454
AOI: AORI_0460
AJA: AJAP_37345(mshA)
AMYC: CU254_01010(mshA)
PDX: Psed_5645
PSEA: WY02_13350
PSEE: FRP1_00890
PSEH: XF36_00290
AMI: Amir_6757
APRE: CNX65_33310(mshA)
SESP: BN6_81180(gtuS4-21)
KAL: KALB_8383
ACTI: UA75_02230
ACAD: UA74_02220
AHG: AHOG_02170(mshA1)
ACTA: C1701_12670(mshA)
ALL: CRK60232
SAQ: Sare_0398
MIL: ML5_2816
AMS: AMIS_76040(mshA)
ASE: ACPL_7885
ACTN: L083_7672
AFS: AFR_40315
ACTS: ACWT_7754
PLK: CIK06_26200(mshA)
PLAB: C6361_11465(mshA)
PLAT: C6W10_08560(mshA)
SNA: Snas_5953
TBI: Tbis_0494
AEY: CDG81_20995(mshA)
ABAM: B1s21122_05810(mshA)
AFO: Afer_0358
AYM: YM304_37900(mshA)
DET: DET1002
DEH: cbdbA963
DEV: DhcVS_874
DMC: btf_951
DMD: dcmb_937
DMG: GY50_0881
DEW: DGWBC_1264(mshA)
DFO: Dform_01228(mshA)
RCA: Rcas_2209
CAU: Caur_1317
CAG: Cagg_1960
HAU: Haur_2249
TRO: trd_0418
STI: Sthe_1464
ATM: ANT_01730
PBF: CFX0092_A0267(mshA)
TTR: Tter_0868
 » show all
Taxonomy
Reference
1  [PMID:12754249]
  Authors
Newton GL, Koledin T, Gorovitz B, Rawat M, Fahey RC, Av-Gay Y
  Title
The glycosyltransferase gene encoding the enzyme catalyzing the first step of mycothiol biosynthesis (mshA).
  Journal
J Bacteriol 185:3476-9 (2003)
DOI:10.1128/JB.185.11.3476-3479.2003
  Sequence
[msm:MSMEG_0933] [mtu:Rv0486]
Reference
2  [PMID:16940050]
  Authors
Newton GL, Ta P, Bzymek KP, Fahey RC
  Title
Biochemistry of the initial steps of mycothiol biosynthesis.
  Journal
J Biol Chem 281:33910-20 (2006)
DOI:10.1074/jbc.M604724200
  Sequence
Reference
3  [PMID:18390549]
  Authors
Vetting MW, Frantom PA, Blanchard JS
  Title
Structural and enzymatic analysis of MshA from Corynebacterium glutamicum: substrate-assisted catalysis.
  Journal
J Biol Chem 283:15834-44 (2008)
DOI:10.1074/jbc.M801017200
  Sequence
[cgl:NCgl0389]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.250
IUBMB Enzyme Nomenclature: 2.4.1.250
ExPASy - ENZYME nomenclature database: 2.4.1.250
BRENDA, the Enzyme Database: 2.4.1.250

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