KEGG   ENZYME: 2.4.1.255Help
Entry
EC 2.4.1.255                Enzyme                                 

Name
protein O-GlcNAc transferase;
O-GlcNAc transferase;
OGTase;
O-linked N-acetylglucosaminyltransferase;
uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase;
protein O-linked beta-N-acetylglucosamine transferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-alpha-acetyl-D-glucosamine:[protein]-3-O-N-acetyl-beta-D-glucosaminyl transferase
Reaction(IUBMB)
(1) UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine [RN:R09676];
(2) UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine [RN:R09677]
Reaction(KEGG)
R09676 R09677;
(other) R09304(G)
Show
Substrate
UDP-N-acetyl-alpha-D-glucosamine [CPD:C00043];
[protein]-L-serine [CPD:C02189];
[protein]-L-threonine [CPD:C19803]
Product
UDP [CPD:C00015];
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine [CPD:C19802];
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine [CPD:C19804]
Comment
Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.
History
EC 2.4.1.255 created 2011
Pathway
ec00514  Other types of O-glycan biosynthesis
Orthology
K09667  protein O-GlcNAc transferase
K18134  EGF domain-specific O-GlcNAc transferase
Genes
HSA: 285203(EOGT) 8473(OGT)
PTR: 465702(OGT) 494220(EOGT)
PPS: 100970968(EOGT) 100989146(OGT)
GGO: 101130943(EOGT) 101140533(OGT)
PON: 100174354(OGT) 100440288(EOGT)
NLE: 100591966(OGT) 100597324(EOGT)
MCC: 696742(EOGT) 698268(OGT)
MCF: 101866582(OGT) 102115709(EOGT)
CSAB: 103227871(EOGT) 103232164(OGT)
RRO: 104664903(OGT) 104677919(EOGT)
RBB: 108520117(EOGT) 108522675 108527587(OGT)
CJC: 100386886(OGT) 100390811(EOGT)
SBQ: 101045971(OGT) 101048286(EOGT)
MMU: 101351(Eogt) 108155(Ogt)
RNO: 26295(Ogt) 494219(Eogt)
CGE: 100757071(Eogt) 100768670(Ogt)
NGI: 103729142(Eogt) 103751946(Ogt)
HGL: 101710902 101715607(Eogt) 101721730(Ogt)
CCAN: 109691372(Eogt) 109699231(Ogt)
OCU: 100342123(EOGT) 100354727(OGT)
TUP: 102479359(OGT) 102499112(EOGT)
CFA: 480955(OGT) 494221(EOGT)
AML: 100475282(OGT) 100479105(EOGT)
UMR: 103674238(EOGT) 103682584(OGT)
ORO: 101369290(OGT) 101370331(EOGT)
FCA: 101089680(EOGT) 101094162(OGT)
PTG: 102959921(EOGT) 102964072(OGT)
AJU: 106975137(EOGT) 106982362(OGT)
BTA: 508782(EOGT) 532053(OGT)
BOM: 102279900(EOGT) 102286677(OGT)
PHD: 102328809(EOGT) 102344387(OGT)
CHX: 102176741(EOGT) 102187305(OGT)
OAS: 101106996(OGT) 101117114(EOGT)
SSC: 100155485(EOGT) 664652(OGT)
CFR: 102516733(OGT) 102521209(EOGT)
CDK: 105087990(OGT) 105095255(EOGT)
BACU: 103018912(EOGT) 103019745 103020800(OGT)
LVE: 103071794(EOGT) 103073601(OGT)
OOR: 101276173(EOGT) 101282914(OGT)
ECB: 100053493(EOGT) 100056148(OGT)
EPZ: 103542615(OGT) 103550858(EOGT)
EAI: 106827921(EOGT) 106828870(OGT)
MYB: 102245549(OGT) 102247874(EOGT)
MYD: 102751826(EOGT) 102773180(OGT)
HAI: 109385597(OGT) 109392531(EOGT)
RSS: 109434630(EOGT) 109441729 109455993(OGT)
PALE: 102879009(EOGT) 102890872(OGT)
LAV: 100662852(OGT) 100671086(EOGT)
MDO: 100011073(OGT) 100618477(EOGT)
SHR: 100929019(OGT) 100931735(EOGT)
OAA: 100078373(EOGT)
GGA: 422203(OGT) 426961(EOGT)
MGP: 100538830(OGT) 100550764(EOGT)
CJO: 107312490(OGT) 107319994(EOGT)
APLA: 101796788(EOGT) 101799851(OGT)
ACYG: 106035493(EOGT) 106037709(OGT)
TGU: 100224237(OGT) 100225538(EOGT)
GFR: 102042181(EOGT) 102044727(OGT)
FAB: 101808611(OGT) 101809614(EOGT)
PHI: 102112943(OGT) 102113990(EOGT)
PMAJ: 107203795(OGT) 107210283(EOGT)
CCAE: 111929384(OGT) 111934977(EOGT)
CCW: 104683359(EOGT) 104691638(OGT)
FPG: 101915918(OGT) 101923329(EOGT)
FCH: 102050428(EOGT) 102052264(OGT)
CLV: 102092523(OGT) 102092951(EOGT)
EGZ: 104124274(OGT) 104132663(EOGT)
AAM: 106482679(OGT) 106494570(EOGT)
ASN: 102381905(EOGT) 102387350(OGT)
AMJ: 102575029(OGT) 102576718(EOGT)
PSS: 102445994(OGT) 102448245(EOGT)
CPIC: 101937402(OGT) 101947597(EOGT)
ACS: 100554805(eogt) 100561277(ogt)
PVT: 110080200(OGT) 110083322(EOGT)
PBI: 103048321(EOGT) 103059316(OGT)
GJA: 107108561(OGT) 107121813(EOGT)
XLA: 443983(eogt.L) 447694(ogt.L)
XTR: 553157(ogt) 780148(eogt)
NPR: 108786181(EOGT) 108802573(OGT)
DRE: 337685(ogt.1) 652952(ogt.2) 798087(eogt)
IPU: 108254783(eogt) 108268666(ogt)
AMEX: 103028765(ogt) 103044264(eogt)
TRU: 101076057(ogt) 777950(eogt)
LCO: 104923189(ogt) 104933151(eogt)
MZE: 101479094(ogt) 101484112(eogt)
OLA: 101164571(ogt) 101170339(eogt)
XMA: 102227742(ogt) 102236401(eogt)
PRET: 103465051(eogt) 103471170(ogt)
NFU: 107385431(eogt) 107395869(ogt)
KMR: 108240572(ogt) 108244079(eogt)
CSEM: 103386183(eogt) 103391144(ogt)
LCF: 108882891(ogt) 108885653(eogt)
SDU: 111221765(eogt) 111226555(ogt)
HCQ: 109509878(eogt) 109515817(ogt)
BPEC: 110163678(ogt) 110165803(eogt)
MALB: 109956143(eogt) 109968379(ogt)
ELS: 105017304(eogt) 105025795(ogt)
LCM: 102352761(EOGT) 102361281(OGT)
CMK: 103179783(eogt) 103189357(ogt)
CIN: 100176976 100181206(ogt) 497241(aer61)
SPU: 579289
DME: Dmel_CG10392(sxc) Dmel_CG9867(Eogt)
DSI: Dsimw501_GD17889(Dsim_GD17889) Dsimw501_GD23152(Dsim_GD23152) Dsimw501_GD28381(Dsim_GD28381)
NVI: 100114611 100116809(Ogt)
CEL: CELE_H12D21.10(H12D21.10) CELE_K04G7.3(ogt-1)
CBR: CBG16605(Cbr-ogt-1)
BMY: Bm1_01735
SHX: MS3_06840
EGL: EGR_08361
HMG: 100201589
ATH: AT3G04240(SEC) AT3G11540(SPY)
LJA: Lj5g3v0803050.1(Lj5g3v0803050.1) Lj5g3v1303350.1(Lj5g3v1303350.1) Lj5g3v1303350.2(Lj5g3v1303350.2)
DOSA: Os02t0489550-00(Os02g0489550) Os08t0559300-01(Os08g0559300)
ATS: 109756755 109763129(LOC109763129) 109776406 109782465(LOC109782465)
ZMA: 100191509(pco141552) 100381672(cl27878_1(278))
CRE: CHLREDRAFT_117813(OGT1)
MNG: MNEG_0970
OLU: OSTLU_863
MAJ: MAA_03264
CMT: CCM_09057
ABP: AGABI1DRAFT78054(AGABI1DRAFT_78054)
DDI: DDB_G0285263(spyA)
DFA: DFA_05779(spyA)
SMIN: v1.2.002533.t1(symbB.v1.2.002533.t1) v1.2.010212.t1(symbB.v1.2.010212.t1) v1.2.015572.t1(symbB.v1.2.015572.t1) v1.2.015572.t2(symbB.v1.2.015572.t2) v1.2.021970.t1(symbB.v1.2.021970.t1) v1.2.023338.t1(symbB.v1.2.023338.t1) v1.2.023339.t1(symbB.v1.2.023339.t1) v1.2.023339.t2(symbB.v1.2.023339.t2)
SPAR: SPRG_08133
SLIM: SCL_1574
SVA: SVA_1487
CABY: Cabys_826(pilF)
 » show all
Taxonomy
Reference
1  [PMID:18948359]
  Authors
Banerjee S, Robbins PW, Samuelson J
  Title
Molecular characterization of nucleocytosolic O-GlcNAc transferases of Giardia lamblia and Cryptosporidium parvum.
  Journal
Glycobiology 19:331-6 (2009)
DOI:10.1093/glycob/cwn107
  Sequence
Reference
2  [PMID:18818698]
  Authors
Clarke AJ, Hurtado-Guerrero R, Pathak S, Schuttelkopf AW, Borodkin V, Shepherd SM, Ibrahim AF, van Aalten DM
  Title
Structural insights into mechanism and specificity of O-GlcNAc transferase.
  Journal
EMBO J 27:2780-8 (2008)
DOI:10.1038/emboj.2008.186
Reference
3  [PMID:16541109]
  Authors
Rao FV, Dorfmueller HC, Villa F, Allwood M, Eggleston IM, van Aalten DM
  Title
Structural insights into the mechanism and inhibition of eukaryotic O-GlcNAc hydrolysis.
  Journal
EMBO J 25:1569-78 (2006)
DOI:10.1038/sj.emboj.7601026
Reference
4  [PMID:1533623]
  Authors
Haltiwanger RS, Blomberg MA, Hart GW
  Title
Glycosylation of nuclear and cytoplasmic proteins. Purification and characterization of a uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase.
  Journal
J Biol Chem 267:9005-13 (1992)
Reference
5  [PMID:9083068]
  Authors
Lubas WA, Frank DW, Krause M, Hanover JA
  Title
O-Linked GlcNAc transferase is a conserved nucleocytoplasmic protein containing tetratricopeptide repeats.
  Journal
J Biol Chem 272:9316-24 (1997)
DOI:10.1074/jbc.272.14.9316
  Sequence
[cel:CELE_K04G7.3] [hsa:8473]
Reference
6  [PMID:21240259]
  Authors
Lazarus MB, Nam Y, Jiang J, Sliz P, Walker S
  Title
Structure of human O-GlcNAc transferase and its complex with a peptide substrate.
  Journal
Nature 469:564-7 (2011)
DOI:10.1038/nature09638
  Sequence
[hsa:8473]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.255
IUBMB Enzyme Nomenclature: 2.4.1.255
ExPASy - ENZYME nomenclature database: 2.4.1.255
BRENDA, the Enzyme Database: 2.4.1.255

DBGET integrated database retrieval system