KEGG   ENZYME: 2.4.1.258Help
Entry
EC 2.4.1.258                Enzyme                                 

Name
dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase;
Man5GlcNAc2-PP-Dol mannosyltransferase;
ALG3;
dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase;
Not56-like protein;
Alg3 alpha-1,3-mannosyl transferase;
Dol-P-Man:Man5GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase;
dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-mannosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dolichyl beta-D-mannosyl phosphate:alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol 3-alpha-D-mannosyltransferase (configuration-inverting)
Reaction(IUBMB)
dolichyl beta-D-mannosyl phosphate + alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol = alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06258]
Reaction(KEGG)
R06258(G)
Show
Substrate
dolichyl beta-D-mannosyl phosphate [CPD:C03862];
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
Product
alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:C00110]
Comment
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-dolichol to Man9Glc-NAc2-PP-dolichol on the lumenal side use dolichyl beta-D-mannosyl phosphate. The first step of this assembly pathway on the luminal side of the endoplasmic reticulum is catalysed by ALG3.
History
EC 2.4.1.258 created 1976 as EC 2.4.1.130, part transferred 2011 to EC 2.4.1.258, modified 2012
Pathway
ec00510  N-Glycan biosynthesis
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K03845  alpha-1,3-mannosyltransferase
Genes
HSA: 10195(ALG3)
PTR: 460886(ALG3)
PPS: 100979287(ALG3)
GGO: 101148766(ALG3)
PON: 100448751(ALG3)
NLE: 100581760(ALG3)
MCC: 703087(ALG3)
MCF: 102134126(ALG3)
CSAB: 103221139(ALG3)
RRO: 104664632(ALG3)
RBB: 108524997(ALG3)
CJC: 100390805(ALG3)
SBQ: 101045776(ALG3)
MMU: 208624(Alg3)
RNO: 287983(Alg3)
CGE: 100772003(Alg3)
NGI: 103732650(Alg3)
HGL: 101714764(Alg3)
CCAN: 109694096(Alg3)
OCU: 100358108(ALG3)
TUP: 102473222(ALG3)
CFA: 478653(ALG3)
AML: 100472334(ALG3)
UMR: 103676182(ALG3)
ORO: 101377982(ALG3)
FCA: 101087681(ALG3)
PTG: 102971503(ALG3)
AJU: 106983810(ALG3)
BTA: 614624(ALG3)
BOM: 102285313(ALG3)
BIU: 109557412(ALG3)
PHD: 102320266(ALG3)
CHX: 102181899(ALG3)
OAS: 101121650(ALG3)
SSC: 100627022(ALG3)
CFR: 102504086(ALG3)
CDK: 105085886(ALG3)
BACU: 103005524(ALG3)
LVE: 103084099(ALG3)
OOR: 101272032(ALG3)
ECB: 100059007(ALG3)
EPZ: 103554746(ALG3)
EAI: 106834523(ALG3)
MYB: 102241054(ALG3)
MYD: 102766472(ALG3)
HAI: 109396699(ALG3)
RSS: 109449490(ALG3)
PALE: 102890134(ALG3)
LAV: 100674341(ALG3)
TMU: 101360342
MDO: 100025876(ALG3)
SHR: 100932630(ALG3)
OAA: 100681362
GGA: 424952(ALG3)
MGP: 100543165(ALG3)
CJO: 107318207(ALG3)
APLA: 101791055(ALG3)
ACYG: 106032204(ALG3)
TGU: 100230731(ALG3)
GFR: 102039749(ALG3)
FAB: 101819823(ALG3)
PHI: 102102878(ALG3)
PMAJ: 107208518(ALG3)
CCAE: 111933602(ALG3)
CCW: 104688139(ALG3)
FPG: 101916756(ALG3)
FCH: 102050162(ALG3)
CLV: 102094667(ALG3)
EGZ: 104122229(ALG3)
AAM: 106500262(ALG3)
ASN: 102369785(ALG3)
AMJ: 102560066(ALG3)
PSS: 102457770(ALG3)
CMY: 102937300(ALG3)
CPIC: 101941423(ALG3)
ACS: 100561775(alg3)
PVT: 110091600(ALG3)
PBI: 103048200(ALG3)
GJA: 107123667(ALG3)
XLA: 100037243(alg3.S)
XTR: 100127746(alg3)
NPR: 108804700(ALG3)
DRE: 553725(alg3)
CCAR: 109063669(alg3)
IPU: 108280746(alg3)
AMEX: 103037087(alg3)
TRU: 101070707(alg3)
LCO: 104935094(alg3)
NCC: 104957796(alg3)
MZE: 101479101(alg3)
OLA: 101167399(alg3)
XMA: 102230775(alg3)
PRET: 103479375(alg3)
NFU: 107384062(alg3)
KMR: 108239541(alg3)
CSEM: 103395988(alg3)
LCF: 108883543(alg3)
SDU: 111229092(alg3)
HCQ: 109524102(alg3)
BPEC: 110158566(alg3)
MALB: 109957667(alg3)
SASA: 106600580(alg3)
ELS: 105021055(alg3)
SFM: 108935289(alg3)
LCM: 102361691(ALG3)
CMK: 103182887(alg3)
CIN: 100176327
SPU: 576672
APLC: 110978953
SKO: 100370088
DME: Dmel_CG4084(l(2)not)
DSI: Dsimw501_GD25022(Dsim_GD25022)
DVI: Dvir_GJ18303(Dvir_l(2)not)
MDE: 101887633
AAG: 5574793
AME: 724399
BIM: 100740988
BTER: 100643648
SOC: 105199092
AEC: 105152598
ACEP: 105617243
PBAR: 105433742
HST: 105182205
DQU: 106748045
CFO: 105251420
LHU: 105668510
PGC: 109859340
PCF: 106784472
NVI: 100115752
MDL: 103575039
TCA: 655451
DPA: 109539990
NVL: 108558978
BMOR: 101741427
PMAC: 106712323
PRAP: 111002964
HAW: 110384151
API: 103308067
DNX: 107172979
CLEC: 106663453
ZNE: 110833535
FCD: 110846318
CEL: CELE_K09E4.2(K09E4.2)
CBR: CBG20997
TSP: Tsp_08712
CRG: 105346658
OBI: 106874294
LAK: 106174922
SHX: MS3_09308
EPA: 110251455
ADF: 107332168
HMG: 100214877
ATH: AT2G47760(ALG3)
CRB: 17890265
BRP: 103866347
BOE: 106337396
THJ: 104802172
CPAP: 110821460
CIT: 102617688
TCC: 18613533
GRA: 105762674
DZI: 111314136
EGR: 104456792
VRA: 106758012
CCAJ: 109812263
CAM: 101509815
LJA: Lj1g3v4899050.1(Lj1g3v4899050.1)
ADU: 107492492
AIP: 107645621
FVE: 101310494
PPER: 18767523
PMUM: 103333910
PAVI: 110761861
ZJU: 107426367
CSV: 101211394
CMO: 103492832
MCHA: 111020823
CMAX: 111488806
CMOS: 111456562
CPEP: 111807027
RCU: 8271753
JCU: 105642496
HBR: 110660934
POP: 7457948
VVI: 100253790
SLY: 101251511
SPEN: 107029293
SOT: 102598887
CANN: 107864015
NSY: 104225504
NTO: 104099176
INI: 109160269
SIND: 105165232
OEU: 111394355
LSV: 111917946
CCAV: 112513534
DCR: 108224117
BVG: 104906606
SOE: 110797544
NNU: 104610985
OSA: 9266078
DOSA: Os01t0172000-01(Os01g0172000)
OBR: 102705966
BDI: 100822122
ATS: 109779517(LOC109779517)
SBI: 8056549
ZMA: 100276684
SITA: 101768406
PDA: 103712729
EGU: 105060271
MUS: 103981289
DCT: 110101639
PEQ: 110026944
AOF: 109841030
ATR: 18430288
SMO: SELMODRAFT_96090(GT58A1)
PPP: 112287567
APRO: F751_3769
SCE: YBL082C(ALG3)
ERC: Ecym_7147
KMX: KLMA_80379(ALG3)
NCS: NCAS_0E02510(NCAS0E02510)
NDI: NDAI_0E04080(NDAI0E04080)
TPF: TPHA_0K00760(TPHA0K00760)
TBL: TBLA_0D00510(TBLA0D00510)
TDL: TDEL_0C02670(TDEL0C02670)
KAF: KAFR_0L01850(KAFR0L01850)
PIC: PICST_58095(RHK1)
CAL: CAALFM_C604340WA(CaO19.1092)
CAUR: QG37_08090
NCR: NCU06552
NTE: NEUTE1DRAFT78420(NEUTE1DRAFT_78420)
MGR: MGG_08010
SSCK: SPSK_09197
MAW: MAC_01438
MAJ: MAA_07040
CMT: CCM_00021
BFU: BCIN_12g04720(Bcalg3)
MBE: MBM_00831
ANI: AN0104.2
ANG: ANI_1_1172164(An18g02360)
ABE: ARB_04378
TVE: TRV_03168
PNO: SNOG_16505(SNOG_16504)
PTE: PTT_10389
SPO: SPAC7D4.06c(alg3)
CNE: CNL04800
CNB: CNBI2040
LBC: LACBIDRAFT_247511(ALG3)
SCM: SCHCODRAFT_51393(alg3)
ABP: AGABI1DRAFT118456(AGABI1DRAFT_118456)
ABV: AGABI2DRAFT200258(AGABI2DRAFT_200258)
DDI: DDB_G0268238(alg3)
DFA: DFA_08406 DFA_10860(alg3)
SMIN: v1.2.013786.t1(symbB.v1.2.013786.t1)
FCY: FRACYDRAFT_180557(ALG3)
SPAR: SPRG_04773
EHX: EMIHUDRAFT_426313(ALG3-1) EMIHUDRAFT_521974(ALG3-2)
LMA: LMJF_36_2040(ALG3)
LIF: LINJ_36_2160(ALG3)
 » show all
Taxonomy
Reference
1  [PMID:11308030]
  Authors
Sharma CB, Knauer R, Lehle L
  Title
Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.
  Journal
Biol Chem 382:321-8 (2001)
DOI:10.1515/BC.2001.039
  Sequence
[sce:YBL082C]
Reference
2  [PMID:10660594]
  Authors
Cipollo JF, Trimble RB
  Title
The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm  addition in downstream oligosaccharide-lipid and glycoprotein glycan processing.
  Journal
J Biol Chem 275:4267-77 (2000)
DOI:10.1074/jbc.275.6.4267
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.258
IUBMB Enzyme Nomenclature: 2.4.1.258
ExPASy - ENZYME nomenclature database: 2.4.1.258
BRENDA, the Enzyme Database: 2.4.1.258

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