KEGG   ENZYME: 2.4.1.266Help
Entry
EC 2.4.1.266                Enzyme                                 

Name
glucosyl-3-phosphoglycerate synthase;
GpgS protein;
GPG synthase;
glucosylphosphoglycerate synthase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
NDP-glucose:3-phospho-D-glycerate 2-alpha-D-glucosyltransferase
Reaction(IUBMB)
NDP-glucose + 3-phospho-D-glycerate = NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate [RN:R09663]
Reaction(KEGG)
Substrate
NDP-glucose [CPD:C15541];
3-phospho-D-glycerate [CPD:C00197]
Product
NDP [CPD:C00454];
2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate [CPD:C19791]
Comment
The enzyme is involved in biosynthesis of 2-O-(alpha-D-glucopyranosyl)-D-glycerate via the two-step pathway in which glucosyl-3-phosphoglycerate synthase catalyses the conversion of GDP-glucose and 3-phospho-D-glycerate into 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate, which is then converted to 2-O-(alpha-D-glucopyranosyl)-D-glycerate by EC 3.1.3.85 glucosyl-3-phosphoglycerate phosphatase. The activity is dependent on divalent cations (Mn2+, Co2+, or Mg2+). The enzyme from Persephonella marina shows moderate flexibility on the sugar donor concerning the nucleotide moiety (UDP-glucose, ADP-glucose, GDP-glucose) but is strictly specific for glucose. The enzyme is also strictly specific for 3-phospho-D-glycerate as acceptor [1]. The enzyme from Methanococcoides burtonii is strictly specific for GDP-glucose and 3-phospho-D-glycerate [2]. This enzyme catalyses the first glucosylation step in methylglucose lipopolysaccharide biosynthesis in mycobacteria [4,5].
History
EC 2.4.1.266 created 2011
Orthology
K13693  glucosyl-3-phosphoglycerate synthase
Genes
GQU: AWC35_23990
SECT: A359_01060
KOR: AWR26_09480
KRD: A3780_14500
PSTS: E05_33790
SPE: Spro_2340
SRR: SerAS9_2300
SRL: SOD_c21660(gpgS)
SRY: M621_11875
SPLY: Q5A_012060(gpgS)
SMAF: D781_2192
RAA: Q7S_11385
ECA: ECA2249
PATR: EV46_10800
PATO: GZ59_23120
PCT: PC1_2052
SOD: Sant_1649
DDQ: DDI_1964
ETA: ETA_20050
EPY: EpC_21280
EPR: EPYR_02288(wcaA)
EAM: EAMY_1494(wcaA)
EAY: EAM_1476
EBI: EbC_26750
EGE: EM595_2252(gpgS)
PAM: PANA_2314
PAJ: PAJ_1624
PVA: Pvag_1805(wcaa3)
PSTW: DSJ_11870
SFR: Sfri_2847
CPS: CPS_0507
PAT: Patl_2163
PSM: PSM_B0463
PSEO: OM33_12575
PSPO: PSPO_a2034
PART: PARC_b0578
MHC: MARHY2496
MAD: HP15_1147
AMAL: I607_10650
AMAE: I876_10980
AMAO: I634_10850
AMAD: I636_10870
AMAI: I635_11295
AMAG: I533_10605
AMAC: MASE_10295
AAUS: EP12_11405
GPS: C427_3015
PIN: Ping_2601
FBL: Fbal_3158
MVS: MVIS_2665
SDE: Sde_3209
SAGA: M5M_05060
MICC: AUP74_03225(gpgS)
TCX: Tcr_0773
MEC: Q7C_403
HCH: HCH_00270
CSA: Csal_2603
HAM: HALO3288
TOL: TOL_2108
SALN: SALB1_1008
PSPI: PS2015_704
DOL: Dole_2860
DAL: Dalk_4108
SAT: SYN_00867
DBR: Deba_0864
MMED: Mame_02360(gpgS)
RDE: RD1_3714
DSH: Dshi_1821
CID: P73_0394
MALG: MALG_02975
RSU: NHU_00526
RHC: RGUI_0581
SPSE: SULPSESMR1_00022(gpgS)
RMM: ROSMUCSMR3_02716(gpgS)
AHT: ANTHELSMS3_00038(gpgS)
HBA: Hbal_1871
SFLA: SPHFLASMR4Y_01596(gpgS)
TXI: TH3_11215
MTU: Rv1208(gpgS)
MTV: RVBD_1208
MTC: MT1246
MRA: MRA_1217
MTUR: CFBS_1287
MTD: UDA_1208
MTUC: J113_08475
MTUE: J114_06515
MTUL: TBHG_01191
MTUT: HKBT1_1285
MTUU: HKBT2_1291
MBO: BQ2027_MB1240(gpgs)
MBB: BCG_1268
MBT: JTY_1243
MBX: BCGT_1039
MAF: MAF_12270
MMIC: RN08_1351
MLE: ML1064
MLB: MLBr01064
MPA: MAP_2569c
MAO: MAP4_1250
MAVI: RC58_06185
MAVU: RE97_06180
MAV: MAV_1353
MIT: OCO_12620
MIA: OCU_12580
MID: MIP_02004
MYO: OEM_12760
MIR: OCQ_12600
MLP: MLM_1276
MUL: MUL_0959
MMC: Mmcs_4005
MKM: Mkms_4079
MJL: Mjls_4235
MMI: MMAR_4230
MMM: W7S_06145
MHAD: B586_07660
MVA: Mvan_4508
MGI: Mflv_2187
MPHL: MPHLCCUG_04048(gpgS)
MVQ: MYVA_4285
MAB: MAB_1346
MABB: MASS_1346
MCHE: BB28_06645
MSTE: MSTE_01312
ASD: AS9A_3352
CGL: NCgl1067(Cgl1112)
CGB: cg1263
CGU: WA5_1067
CGT: cgR_1196
CGM: cgp_1263
CGJ: AR0_06060
CEF: CE1169
CDI: DIP0985
CDIP: ERS451417_00888(crtQ)
COP: Cp31_0790
CPSE: CPTA_01337
CPSU: CPTB_00195
CPSF: CPTC_01327
CMV: CMUST_06045(gpgS)
CSP: WM42_0378
CPHO: CPHO_07855
CGV: CGLAU_04915(gpgS)
CAMG: CAMM_08515
CMIN: NCTC10288_01549(crtQ)
NFA: NFA_47360
NSR: NS506_01918(gpgS)
RER: RER_41660(gpgS)
REY: O5Y_19470
ROP: ROP_60290(gpgS)
REQ: REQ_14380
RFA: A3L23_02783(gpgS_1) A3L23_03104(gpgS_2)
RHS: A3Q41_00225(gpgS_1) A3Q41_00577(gpgS_2)
RHU: A3Q40_02311(gpgS)
RRT: 4535765_01343(crtQ_1)
GBR: Gbro_3310
GOR: KTR9_3350
TPR: Tpau_1165
SRT: Srot_1588
SCO: SCO4292(SCD95A.25c)
SALB: XNR_2397
SGR: SGR_3229
SGB: WQO_19545
SCT: SCAT_2451
SFA: Sfla_2795
SBH: SBI_04950
SHY: SHJG_4826
SVE: SVEN_4045
SALS: SLNWT_4201
STRP: F750_4001
SFI: SFUL_4092
SALU: DC74_4782
SALL: SAZ_25600
SLV: SLIV_16935(gpgS)
SGU: SGLAU_15430(gpgS)
STRE: GZL_04046
SLD: T261_3419
STRM: M444_16215
SAMB: SAM23877_3414(gpgS)
SPRI: SPRI_4107
SRW: TUE45_04178(gpgS)
SLE: sle_39110(sle_39110)
SRN: A4G23_02441(gpgS)
STRD: NI25_21785
SMAL: SMALA_3468
SLAU: SLA_4120
SALJ: SMD11_2841
SLX: SLAV_17490(gpgS)
SFK: KY5_3673
KSK: KSE_44070
NCA: Noca_4157
NDK: I601_1791(gpgS)
PSIM: KR76_22485
TCU: Tcur_2488
SRO: Sros_3452
MMAR: MODMU_0394
KRA: Krad_0167
PDX: Psed_1018
PSEE: FRP1_22050
KAL: KALB_946
MIL: ML5_5524
ACTN: L083_5153
AFS: AFR_17355
CAI: Caci_8126
TBI: Tbis_1863
RXY: Rxyl_2312
CWO: Cwoe_5332
AYM: YM304_16830(gpgS)
SYW: SYNW2436
SYG: sync_0360
SYNR: KR49_08495
SYND: KR52_02930
SYNW: SynWH8103_02816(gpgS)
HHG: XM38_048050(gpgS)
PMA: Pro_0727(wcaA)
PMM: PMM0962
PRC: EW14_0925
PRM: EW15_0930
AMR: AM1_3648
CAA: Caka_1159
XII: AMD24_00147(gpgS)
RBA: RB1005
FMR: Fuma_02599(gpgS)
KST: KSMBR1_2373(gpgS_1) KSMBR1_2962(gpgS_2)
VBL: L21SP4_00224(gpgS)
ABAC: LuPra_00692(gpgS)
DORI: FH5T_18345
PMO: Pmob_1142
CABY: Cabys_737
NDE: NIDE1666
NIO: NITINOP_2744(gpgS)
NJA: NSJP_3836(gpgS)
MOX: DAMO_1541
MBU: Mbur_0737(gpgS)
MMET: MCMEM_1377
MMH: Mmah_1335
MPY: Mpsy_2707
HAL: VNG_0271C
HSL: OE_1422F(gtl5)
HHB: Hhub_1426
HSU: HLASF_0602(gtl)
HSF: HLASA_0598(gtl)
NPH: NP_5222A(gtl5)
NMO: Nmlp_1894(gtl5)
HUT: Huta_1195
HTI: HTIA_1115
HMU: Hmuk_3045
HWA: HQ_1180A(gtl5)
HWC: Hqrw_1222(gtl5)
HVO: HVO_0760
HME: HFX_0717(wcaA)
HLA: Hlac_1456
HTU: Htur_1287
NMG: Nmag_2620
NAT: NJ7G_4120
SALI: L593_09240
 » show all
Taxonomy
Reference
1  [PMID:17189358]
  Authors
Costa J, Empadinhas N, da Costa MS
  Title
Glucosylglycerate biosynthesis in the deepest lineage of the Bacteria: characterization of the thermophilic proteins GpgS and GpgP from Persephonella marina.
  Journal
J Bacteriol 189:1648-54 (2007)
DOI:10.1128/JB.00841-06
  Sequence
Reference
2  [PMID:16428406]
  Authors
Costa J, Empadinhas N, Goncalves L, Lamosa P, Santos H, da Costa MS
  Title
Characterization of the biosynthetic pathway of glucosylglycerate in the archaeon Methanococcoides burtonii.
  Journal
J Bacteriol 188:1022-30 (2006)
DOI:10.1128/JB.188.3.1022-1030.2006
  Sequence
[ag:AAZ95006] [mbu:Mbur_0737]
Reference
3  [PMID:18221489]
  Authors
Empadinhas N, Albuquerque L, Mendes V, Macedo-Ribeiro S, da Costa MS
  Title
Identification of the mycobacterial glucosyl-3-phosphoglycerate synthase.
  Journal
FEMS Microbiol Lett 280:195-202 (2008)
DOI:10.1111/j.1574-6968.2007.01064.x
  Sequence
Reference
4  [PMID:19015727]
  Authors
Pereira PJ, Empadinhas N, Albuquerque L, Sa-Moura B, da Costa MS, Macedo-Ribeiro S
  Title
Mycobacterium tuberculosis glucosyl-3-phosphoglycerate synthase: structure of a key enzyme in methylglucose lipopolysaccharide biosynthesis.
  Journal
PLoS One 3:e3748 (2008)
DOI:10.1371/journal.pone.0003748
  Sequence
[mtu:Rv1208]
Reference
5  [PMID:19052364]
  Authors
Gest P, Kaur D, Pham HT, van der Woerd M, Hansen E, Brennan PJ, Jackson M, Guerin ME
  Title
Preliminary crystallographic analysis of GpgS, a key glucosyltransferase involved in methylglucose lipopolysaccharide biosynthesis in Mycobacterium tuberculosis.
  Journal
Acta Crystallogr Sect F Struct Biol Cryst Commun 64:1121-4 (2008)
DOI:10.1107/S1744309108032892
  Sequence
[mtu:Rv1208]
Reference
6  [PMID:19421329]
  Authors
Kaur D, Pham H, Larrouy-Maumus G, Riviere M, Vissa V, Guerin ME, Puzo G, Brennan PJ, Jackson M
  Title
Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria.
  Journal
PLoS One 4:e5447 (2009)
DOI:10.1371/journal.pone.0005447
  Sequence
[mtu:Rv1208] [msm:MSMEG_5084]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.266
IUBMB Enzyme Nomenclature: 2.4.1.266
ExPASy - ENZYME nomenclature database: 2.4.1.266
BRENDA, the Enzyme Database: 2.4.1.266

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