KEGG   ENZYME: 2.4.1.278Help
Entry
EC 2.4.1.278                Enzyme                                 

Name
3-alpha-mycarosylerythronolide B desosaminyl transferase;
EryCIII;
dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose:3-alpha-mycarosylerythronolide B 3-dimethylamino-4,6-dideoxy-alpha-D-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose:3-alpha-mycarosylerythronolide B 3-dimethylamino-3,4,6-trideoxy-beta-D-glucosyltransferase
Reaction(IUBMB)
dTDP-D-desosamine + 3-alpha-L-mycarosylerythronolide B = dTDP + erythromycin D [RN:R05531]
Reaction(KEGG)
Substrate
dTDP-D-desosamine [CPD:C11911];
3-alpha-L-mycarosylerythronolide B [CPD:C06630]
Product
dTDP [CPD:C00363];
erythromycin D [CPD:C06633]
Comment
The enzyme is involved in erythromycin biosynthesis.
History
EC 2.4.1.278 created 2012, modified 2014
Pathway
ec00522  Biosynthesis of 12-, 14- and 16-membered macrolides
ec01130  Biosynthesis of antibiotics
Orthology
K14368  3-alpha-mycarosylerythronolide B desosaminyl transferase
Genes
SEN: SACE_0726(eryCIII)
Taxonomy
Reference
1  [PMID:16218575]
  Authors
Yuan Y, Chung HS, Leimkuhler C, Walsh CT, Kahne D, Walker S
  Title
In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides.
  Journal
J Am Chem Soc 127:14128-9 (2005)
DOI:10.1021/ja053704n
  Sequence
[sen:SACE_0726]
Reference
2  [PMID:15303858]
  Authors
Lee HY, Chung HS, Hang C, Khosla C, Walsh CT, Kahne D, Walker S
  Title
Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway.
  Journal
J Am Chem Soc 126:9924-5 (2004)
DOI:10.1021/ja048836f
  Sequence
[sen:SACE_0726]
Reference
3  [PMID:22056329]
  Authors
Moncrieffe MC, Fernandez MJ, Spiteller D, Matsumura H, Gay NJ, Luisi BF, Leadlay PF
  Title
Structure of the glycosyltransferase EryCIII in complex with its activating P450  homologue EryCII.
  Journal
J Mol Biol 415:92-101 (2012)
DOI:10.1016/j.jmb.2011.10.036
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.278
IUBMB Enzyme Nomenclature: 2.4.1.278
ExPASy - ENZYME nomenclature database: 2.4.1.278
BRENDA, the Enzyme Database: 2.4.1.278

DBGET integrated database retrieval system