KEGG   ENZYME: 2.4.1.287Help
Entry
EC 2.4.1.287                Enzyme                                 

Name
rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,4/1,5-galactofuranosyltransferase;
arabinogalactan galactofuranosyl transferase 1;
GlfT1
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-galactofuranose:alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/4-beta-galactofuranosyltransferase (configuration-inverting)
Reaction(IUBMB)
2 UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 2 UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol (overall reaction);
(1a) UDP-alpha-D-galactofuranose + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol = UDP + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol;
(1b) UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol = UDP + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
Reaction(KEGG)
(other) R12078(G) R12079(G)
Show
Substrate
UDP-alpha-D-galactofuranose [CPD:C03733];
alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol [CPD:C20416];
alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol;
beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
Product
UDP [CPD:C00015];
beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol;
beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol;
beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans-octacis-decaprenol
Comment
Isolated from the bacteria Mycobacterium tuberculosis and M. smegmatis, the enzyme has dual beta-(1->4) and beta-(1->5) transferase action. Involved in the formation of the cell wall in mycobacteria.
History
EC 2.4.1.287 created 2012, modified 2017
Pathway
ec00572  Arabinogalactan biosynthesis - Mycobacterium
ec01100  Metabolic pathways
Orthology
K16649  rhamnopyranosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,3/1,4-galactofuranosyltransferase
Genes
MVR: X781_770
ABS: AZOBR_140059 AZOBR_40146
ABQ: ABAZ39_01435 ABAZ39_04995
ABF: AMK58_09060 AMK58_12400
AZT: TSH58p_12970 TSH58p_16510
MTU: Rv3782(glfT1)
MTV: RVBD_3782
MTC: MT3891
MRA: MRA_3822
MTF: TBFG_13816
MTUR: CFBS_4010
MTD: UDA_3782
MTUE: J114_20205
MTUL: TBHG_03720
MTUT: HKBT1_3993
MTUU: HKBT2_4003
MBO: BQ2027_MB3811(glft1)
MBB: BCG_3844
MBT: JTY_3846
MBX: BCGT_3642
MAF: MAF_37970
MMIC: RN08_4171
MCQ: BN44_120190(glfT)
MCV: BN43_90294(glfT)
MCX: BN42_90305(glfT)
MCZ: BN45_110144(glfT)
MLE: ML0113(rfbE)
MLB: MLBr00113(rfbE)
MPA: MAP_0240c(rfbE)
MAO: MAP4_3631
MAVI: RC58_18050
MAVU: RE97_18085
MAV: MAV_0237
MIT: OCO_02420
MIA: OCU_02480
MID: MIP_00575
MYO: OEM_02540
MIR: OCQ_02420
MLP: MLM_0184
MUL: MUL_0091
MMC: Mmcs_4992
MKM: Mkms_5080
MJL: Mjls_5373
MMI: MMAR_5337
MMM: W7S_01190
MHAD: B586_03135
MVA: Mvan_5619
MGI: Mflv_1185
MPHL: MPHLCCUG_00220(glfT1)
MVQ: MYVA_5549
MTHN: 4412656_04377(glfT1)
MAB: MAB_0202c
MABB: MASS_0200
MCHE: BB28_01040
MSTE: MSTE_00201
MTER: 4434518_04024(glfT1)
ASD: AS9A_0147
CGL: NCgl0195(Cgl0198)
CGB: cg0246
CGU: WA5_0195
CGT: cgR_0277
CGM: cgp_0246
CGJ: AR0_01280
CEF: CE0175
CDI: DIP0168
CDIP: ERS451417_00134(glfT1)
CJK: jk2043
CUR: cu1949
CPL: Cp3995_0112(glfT1)
CPG: Cp316_0122(glfT1)
CPP: CpP54B96_0115(glfT1)
CPK: Cp1002_0108(glfT1)
CPQ: CpC231_0109(glfT1)
CPX: CpI19_0110(glfT1)
CPZ: CpPAT10_0108(glfT1)
COR: Cp267_0119(glfT1)
COP: Cp31_0123(glfT1)
COD: Cp106_0111(glfT1)
COS: Cp4202_0108(glfT1)
COI: CpCIP5297_0119(glfT1)
COE: Cp258_0122(glfT1)
COU: Cp162_0115(glfT1)
CPSE: CPTA_00637
CPSU: CPTB_00896
CPSF: CPTC_00558
CRD: CRES_2108
CUN: Cul210932_0158(glfT1)
CUS: CulFRC11_0153(glfT1)
CUQ: Cul210931_0152(glfT1)
CUZ: Cul05146_0158(glfT1)
CUJ: CUL131002_0142c(glfT1)
CVA: CVAR_2917
CTER: A606_00605
COA: DR71_1182(glfT1)
CSX: CSING_00505(glfT1)
CMQ: B840_00815(glfT1)
CKU: UL82_00375(glfT1)
CCJ: UL81_00580(glfT1)
CMV: CMUST_00605(glfT1)
CEI: CEPID_01025(glfT1)
CTED: CTEST_00765(glfT1)
CUT: CUTER_00465(glfT1)
CDX: CDES_01130(glfT1)
CSP: WM42_1255(glfT1)
CPHO: CPHO_00655
CGV: CGLAU_00490(glfT1)
CAQU: CAQU_00445
CAMG: CAMM_01090
CMIN: NCTC10288_02439(glfT1)
NFA: NFA_2170
NFR: ERS450000_04021(glfT1)
RER: RER_02580(glfT)
REY: O5Y_01275
ROP: ROP_39760(glfT)
REQ: REQ_02490
RHB: NY08_3944
RFA: A3L23_02062(glfT1)
RHS: A3Q41_01300(glfT1)
RHU: A3Q40_03619(glfT1)
RRT: 4535765_00264(glfT1)
GBR: Gbro_0276
GOR: KTR9_0274
TPR: Tpau_4102
SRT: Srot_0341
ART: Arth_2615
ARM: ART_1454
ACH: Achl_2348
KRH: KRH_09720(glfT)
NDK: I601_0163(glfT1)
PSIM: KR76_19865
GOB: Gobs_4321
MMAR: MODMU_4716
SEN: SACE_0199
AMD: AMED_0238
AMN: RAM_01205
AMM: AMES_0233
AMZ: B737_0234
AOI: AORI_0244
AJA: AJAP_01270(glfT1)
PDX: Psed_0206
PSEA: WY02_17215
PSEE: FRP1_25555
PSEH: XF36_25000
AMI: Amir_0132
SESP: BN6_01700(gtuS2-4)
KAL: KALB_166
ACTI: UA75_00695
ACAD: UA74_00695
AHG: AHOG_00695(glfT2)
GFL: GRFL_0079
 » show all
Taxonomy
Reference
1  [PMID:16952951]
  Authors
Mikusova K, Belanova M, Kordulakova J, Honda K, McNeil MR, Mahapatra S, Crick DC, Brennan PJ
  Title
Identification of a novel galactosyl transferase involved in biosynthesis of the  mycobacterial cell wall.
  Journal
J Bacteriol 188:6592-8 (2006)
DOI:10.1128/JB.00489-06
  Sequence
[mtu:Rv3782]
Reference
2  [PMID:18055597]
  Authors
Belanova M, Dianiskova P, Brennan PJ, Completo GC, Rose NL, Lowary TL, Mikusova K
  Title
Galactosyl transferases in mycobacterial cell wall synthesis.
  Journal
J Bacteriol 190:1141-5 (2008)
DOI:10.1128/JB.01326-07
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.287
IUBMB Enzyme Nomenclature: 2.4.1.287
ExPASy - ENZYME nomenclature database: 2.4.1.287
BRENDA, the Enzyme Database: 2.4.1.287

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