KEGG   ENZYME: 2.4.1.288Help
Entry
EC 2.4.1.288                Enzyme                                 

Name
galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase;
GlfT2
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase
Reaction(IUBMB)
28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol = 28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
Reaction(KEGG)
(other) R12080(G)
Show
Substrate
UDP-alpha-D-galactofuranose [CPD:C03733];
beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
Product
UDP [CPD:C00015];
[beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
Comment
Isolated from Mycobacterium tuberculosis. The enzyme adds approximately twenty-eight galactofuranosyl residues with alternating 1->5 and 1->6 links forming a galactan domain with approximately thirty galactofuranosyl residues. Involved in the formation of the cell wall in mycobacteria.
History
EC 2.4.1.288 created 2012
Pathway
ec00572  Arabinogalactan biosynthesis - Mycobacterium
ec01100  Metabolic pathways
Orthology
K16650  galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
Genes
MVR: X781_810
HRB: Hrubri_4144
SINB: SIDU_12400
SCLO: SCLO_1028180
MTU: Rv3808c(glfT2)
MTV: RVBD_3808c
MTC: MT3915
MRA: MRA_3848(glfT)
MTF: TBFG_13842
MTB: TBMG_03855
MTUR: CFBS_4037(glfT)
MTO: MTCTRI2_3887(glfT)
MTD: UDA_3808c(glfT)
MTN: ERDMAN_4174(glfT)
MTUC: J113_26670
MTUE: J114_20340
MTUL: TBHG_03746
MTUT: HKBT1_4020(glfT)
MTUU: HKBT2_4030(glfT)
MTQ: HKBS1_4033(glfT)
MBO: BQ2027_MB3838C(glft2)
MBB: BCG_3870c
MBT: JTY_3872
MBX: BCGT_3670
MAF: MAF_38230(glfT)
MMIC: RN08_4202
MCE: MCAN_38271(glfT)
MCQ: BN44_120215(glfT)
MCV: BN43_90323(glfT)
MCX: BN42_90331(glfT)
MCZ: BN45_110169(glfT)
MLE: ML0093
MLB: MLBr00093
MPA: MAP_0212
MAO: MAP4_3659
MAVI: RC58_18195
MAVU: RE97_18230
MAV: MAV_0209
MAVD: NF84_00995
MAVR: LA63_01025
MAVA: LA64_01035
MIT: OCO_02150
MIA: OCU_02190
MID: MIP_00522
MYO: OEM_02240
MIR: OCQ_02130
MLP: MLM_0152
MUL: MUL_4992(glfT)
MMC: Mmcs_5019
MKM: Mkms_5107
MJL: Mjls_5400
MMI: MMAR_5372(glfT)
MMAE: MMARE11_51930(glfT)
MMM: W7S_01055
MLI: MULP_05651(glfT)
MHAD: B586_03010
MVA: Mvan_5649
MGI: Mflv_1160
MPHL: MPHLCCUG_00197(glfT2)
MVQ: MYVA_5575
MTHN: 4412656_04410(glfT2)
MAB: MAB_0171
MABB: MASS_0169
MABO: NF82_00860
MCHE: BB28_00875
MSTE: MSTE_00170
MJD: JDM601_4126(glfT)
MTER: 4434518_04065(glfT)
ASD: AS9A_0099
CGL: NCgl2783(Cgl2882)
CGB: cg3191
CGU: WA5_2783(GlfT)
CGT: cgR_2770
CGM: cgp_3191(glfT)
CGJ: AR0_14000
CEF: CE2714
CDI: DIP2198
CDP: CD241_2087(glfT)
CDH: CDB402_2062(glfT)
CDT: CDHC01_2088(glfT)
CDE: CDHC02_2082(glfT)
CDR: CDHC03_2084(glfT)
CDA: CDHC04_2114(glfT)
CDZ: CD31A_2218(glfT)
CDB: CDBH8_2174(glfT)
CDS: CDC7B_2180(glfT)
CDD: CDCE8392_2102(glfT)
CDW: CDPW8_2163(glfT)
CDV: CDVA01_2010(glfT)
CDIP: ERS451417_02233(glfT)
CJK: jk0130
CUR: cu0152
CUA: CU7111_0158(glfT)
CAR: cauri_2377(glfT)
CPU: cpfrc_01964(glfT)
CPG: Cp316_2020(glfT1)
CPK: Cp1002_1961(glfT1)
CPQ: CpC231_1955(glfT1)
CPX: CpI19_1976(glfT2)
CPZ: CpPAT10_1968(glfT1)
COP: Cp31_1954
COI: CpCIP5297_1990(glfT1B)
CPSE: CPTA_00358
CPSU: CPTB_01171
CPSF: CPTC_00840
CRD: CRES_0527(glfT)
CUL: CULC22_02196(glfT)
CUC: CULC809_02044(glfT)
CUE: CULC0102_2189(glfT)
CUN: Cul210932_2174(glfT)
CUS: CulFRC11_2066(glfT)
CUQ: Cul210931_2128(glfT)
CUZ: Cul05146_2166(glfT)
CUJ: CUL131002_2083c(glfT)
CVA: CVAR_0241(glfT)
CTER: A606_00975
CGY: CGLY_15105(glfT2)
COA: DR71_1179 DR71_969(glfT2)
CSX: CSING_12365(glfT2)
CMQ: B840_11685(glfT2)
CKU: UL82_09980(glfT)
CMV: CMUST_14225(glfT2)
CEI: CEPID_11660(glfT2)
CTED: CTEST_12405(glfT2)
CUT: CUTER_10435(glfT2)
CDX: CDES_12965(glfT2)
CSP: WM42_1635(glfT2)
CPHO: CPHO_11480
CGV: CGLAU_11485(glfT2)
CAQU: CAQU_11920
CAMG: CAMM_11885
CMIN: NCTC10288_00189(glfT)
NFA: NFA_1770(glfT) NFA_2140
NFR: ERS450000_04024(glfT2_2) ERS450000_04062(glfT2_3)
RER: RER_02120(glfT) RER_02550
ROP: ROP_39320(glfT) ROP_39720
RFA: A3L23_02021(glfT2_1) A3L23_02060(glfT2_2)
RHS: A3Q41_01302(glfT2_1) A3Q41_01342(glfT2_2)
RHU: A3Q40_03621(glfT2_1) A3Q40_03648(glfT2_2)
RRT: 4535765_00212(glfT2_1) 4535765_00247(glfT2_2)
GBR: Gbro_0205
GOR: KTR9_0202
TPR: Tpau_0162
SRT: Srot_0570
CMI: CMM_1021(wcqJ)
CMS: CMS0640
CMC: CMN_00984(wcqJ)
MTS: MTES_0550
ART: Arth_2693
ARM: ART_1683
ARX: ARZXY2_1014(glft2)
AAU: AAur_2675
ACH: Achl_2423
RSA: RSal33209_1617(glfT)
KRH: KRH_08980(glfT)
BCV: Bcav_1219
XCE: Xcel_2574
IDO: I598_3326(glfT2)
PFR: PFREUD_00580(gtfA)
PFRE: RM25_0068
NCA: Noca_1402
NDK: I601_0160(glfT2_1)
PSIM: KR76_19845
TCU: Tcur_0636
MMAR: MODMU_4715
KRA: Krad_3887
AMD: AMED_0232
AMN: RAM_01175
AMM: AMES_0227
AMZ: B737_0228
AOI: AORI_0237
SESP: BN6_01660(gtuS2-3)
KAL: KALB_165
ACTI: UA75_00685
ACAD: UA74_00685
AHG: AHOG_00675(glfT1)
BLO: BL0217
BLA: BLA_0860
BBB: BIF_01252
BBC: BLC1_1463
BLV: BalV_1465
BLW: W7Y_1509
BLS: W91_1540
BANI: Bl12_1417
BANL: BLAC_07580
BANM: EN10_07660
BDE: BDP_2042(rfbF)
BDN: BBDE_1924
BBF: BBB_0064(glfT)
BTP: D805_0393
BPSP: AH67_08100
BANG: BBAG_1470
BCAT: BBCT_1544
SIJ: SCIP_0116
PDO: PSDT_1451
CTHE: Chro_3133
LFI: LFML04_0770(glfT)
 » show all
Taxonomy
Reference
1  [PMID:18423586]
  Authors
Rose NL, Zheng RB, Pearcey J, Zhou R, Completo GC, Lowary TL
  Title
Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase.
  Journal
Carbohydr Res 343:2130-9 (2008)
DOI:10.1016/j.carres.2008.03.023
  Sequence
[mtu:Rv3808c]
Reference
2  [PMID:19571009]
  Authors
May JF, Splain RA, Brotschi C, Kiessling LL
  Title
A tethering mechanism for length control in a processive carbohydrate polymerization.
  Journal
Proc Natl Acad Sci U S A 106:11851-6 (2009)
DOI:10.1073/pnas.0901407106
  Sequence
[mtu:Rv3808c]
Reference
3  [PMID:22707726]
  Authors
Wheatley RW, Zheng RB, Richards MR, Lowary TL, Ng KK
  Title
Tetrameric Structure of the GlfT2 Galactofuranosyltransferase Reveals a Scaffold  for the Assembly of Mycobacterial Arabinogalactan.
  Journal
J Biol Chem 287:28132-43 (2012)
DOI:10.1074/jbc.M112.347484
  Sequence
[mtu:Rv3808c]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.288
IUBMB Enzyme Nomenclature: 2.4.1.288
ExPASy - ENZYME nomenclature database: 2.4.1.288
BRENDA, the Enzyme Database: 2.4.1.288

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