| Entry |
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| Name |
pyrimidine-nucleoside phosphorylase;
Py-NPase;
pdp (gene name)
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| Class |
Transferases;
Glycosyltransferases;
Pentosyltransferases
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| Sysname |
pyrimidine-nucleoside:phosphate (2'-deoxy)-alpha-D-ribosyltransferase
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| Reaction(IUBMB) |
(1) uridine + phosphate = uracil + alpha-D-ribose 1-phosphate [RN: R01876];
(2) thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate [RN: R01570];
(3) 2'-deoxyuridine + phosphate = uracil + 2-deoxy-alpha-D-ribose 1-phosphate [RN: R02484]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
uracil [CPD: C00106];
alpha-D-ribose 1-phosphate [CPD: C00620];
thymine [CPD: C00178];
2-deoxy-alpha-D-ribose 1-phosphate [CPD: C00672]
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| Comment |
Unlike EC 2.4.2.3, uridine phosphorylase, and EC 2.4.2.4, thymidine phosphorylase, this enzyme can accept both the ribonucleoside uridine and the 2'-deoxyribonucleosides 2'-deoxyuridine and thymidine [3]. The reaction is reversible, and the enzyme does not distinguish between alpha-D-ribose 1-phosphate and 2-deoxy-alpha-D-ribose 1-phosphate in the synthetic direction.
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| History |
EC 2.4.2.2 created 1961
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| Pathway |
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| Orthology |
| K00756 | pyrimidine-nucleoside phosphorylase |
| K09913 | purine/pyrimidine-nucleoside phosphorylase |
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| Genes |
» show all
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| Reference |
1 |
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| Authors |
Friedkin, M. and Kalckar, H. |
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| Title |
Nucleoside phosphorylases. |
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| Journal |
In: Boyer, P.D., Lardy, H. and Myrback, K. (Eds.), The Enzymes, 2nd ed., vol. 5, Academic Press, New York, 1961, p. 237-255. |
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| Reference |
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| Authors |
Saunders PP, Wilson BA, Saunders GF. |
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| Title |
Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus. |
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| Journal |
J Biol Chem 244:3691-7 (1969) |
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| Reference |
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| Authors |
Hamamoto T, Noguchi T, Midorikawa Y. |
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| Title |
Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2. |
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| Journal |
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| Reference |
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| Authors |
Okuyama K, Hamamoto T, Noguchi T, Midorikawa Y |
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| Title |
Molecular cloning and expression of the pyrimidine nucleoside phosphorylase gene from Bacillus stearothermophilus TH 6-2. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Pugmire MJ, Ealick SE |
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| Title |
The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation. |
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| Journal |
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| Sequence |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 2.4.2.2 |
| ExPASy - ENZYME nomenclature database: | 2.4.2.2 |
| BRENDA, the Enzyme Database: | 2.4.2.2 |
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