KEGG   ENZYME: 2.4.2.43Help
Entry
EC 2.4.2.43                 Enzyme                                 

Name
lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase;
undecaprenyl phosphate-alpha-L-Ara4N transferase;
4-amino-4-deoxy-L-arabinose lipid A transferase;
polymyxin resistance protein PmrK;
arnT (gene name)
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
BRITE hierarchy
Sysname
4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate:lipid IVA 4-amino-4-deoxy-L-arabinopyranosyltransferase
Reaction(IUBMB)
(1) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,octacis-undecaprenyl phosphate [RN:R09781];
(2) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + lipid IVA = lipid IIA + ditrans,octacis-undecaprenyl phosphate [RN:R09773];
(3) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA = 4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + ditrans,octacis-undecaprenyl phosphate [RN:R09774]
Reaction(KEGG)
Substrate
4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate [CPD:C16157];
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A [CPD:C06026];
lipid IVA;
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
Product
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A [CPD:C19890];
ditrans,octacis-undecaprenyl phosphate [CPD:C17556];
lipid IIA [CPD:C19884];
4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
Comment
Integral membrane protein present in the inner membrane of certain Gram negative endobacteria. In strains that do not produce 3-deoxy-D-manno-octulosonic acid (Kdo), the enzyme adds a single arabinose unit to the 1-phosphate moiety of the tetra-acylated lipid A precursor, lipid IVA. In the presence of a Kdo disaccharide, the enzyme primarily adds an arabinose unit to the 4-phosphate of lipid A molecules. The Salmonella typhimurium enzyme can add arabinose units to both positions.
History
EC 2.4.2.43 created 2010, modified 2011
Orthology
K07264  4-amino-4-deoxy-L-arabinose transferase
Genes
ECO: b2257(arnT)
ECJ: JW2251(arnT)
ECD: ECDH10B_2417(arnT)
EBW: BWG_2030(arnT)
ECOK: ECMDS42_1827(arnT)
ECE: Z3515
ECS: ECs3145(arnT)
ECF: ECH74115_3398(arnT)
ETW: ECSP_3134(arnT)
ELX: CDCO157_2908(arnT)
EOJ: ECO26_3247(arnT)
EOI: ECO111_3007(arnT)
EOH: ECO103_2723(arnT)
ECOO: ECRM13514_3013(arnT)
ECOH: ECRM13516_2958(arnT)
ECG: E2348C_2401(arnT)
EOK: G2583_2797(arnT)
ELR: ECO55CA74_13845(arnT)
ESO: O3O_17430(arnT)
ESM: O3M_08155(arnT)
ESL: O3K_08205(arnT)
ECW: EcE24377A_2552(arnT)
ELH: ETEC_2391
ECC: c2799(yfbI)
ECP: ECP_2300
ECI: UTI89_C2539(yfbI)
ECV: APECO1_4304(yfbI)
ECX: EcHS_A2402(arnT)
ECM: EcSMS35_2411(arnT)
ECY: ECSE_2516
ECR: ECIAI1_2334(arnT)
ECQ: ECED1_2724(arnT)
ECK: EC55989_2504(arnT)
ECT: ECIAI39_2404(arnT)
EOC: CE10_2640(arnT)
EUM: ECUMN_2599(arnT)
ECZ: ECS88_2407(arnT)
ELO: EC042_2500(arnT)
ELN: NRG857_11445(arnT)
ESE: ECSF_2137
EBR: ECB_02183(arnT)
EBD: ECBD_1402
EKF: KO11_11400(arnT)
EAB: ECABU_c25910(arnT)
EDJ: ECDH1ME8569_2193(arnT)
EIH: ECOK1_2493(arnT)
ENA: ECNA114_2350(yfbI)
ELU: UM146_05515(arnT)
ELW: ECW_m2448(arnT)
ELL: WFL_11980(arnT)
ELC: i14_2598(arnT)
ELD: i02_2598(arnT)
EBL: ECD_02183(arnT)
EBE: B21_02142(arnT)
ELF: LF82_0139(arnT)
ECOL: LY180_11720(arnT)
ECOI: ECOPMV1_02418(arnT)
ECOJ: P423_12635(arnT)
ECOS: EC958_2595(yfbI)
EFE: EFER_0912(arnT)
EAL: EAKF1_ch3722c(arnT)
STY: STY2531
STT: t0562(pqaB)
SENT: TY21A_02860(arnT)
STM: STM2301(pqaB)
SEO: STM14_2839(arnT)
SEY: SL1344_2270(arnT)
SEM: STMDT12_C23220(arnT)
SEJ: STMUK_2331(arnT)
SEB: STM474_2397(arnT)
SEF: UMN798_2483(pqaB)
SETU: STU288_07880(arnT)
SETC: CFSAN001921_05290(arnT)
SENR: STMDT2_22701(pqaB)
SEND: DT104_23591(pqaB)
SENI: CY43_12330(arnT)
SEEN: SE451236_17700(arnT)
SPT: SPA0562(pqaB)
SEK: SSPA0526
SEI: SPC_1410(pqaB)
SEC: SCH_2302(yfbI)
SENH: CFSAN002069_20340(arnT)
SEEH: SEEH1578_20780(arnT)
SENS: Q786_11345(arnT)
SED: SeD_A2645
SEG: SG2330(pqaB)
SEL: SPUL_0589(pqaB)
SEGA: SPUCDC_0589(pqaB)
SET: SEN2283(pqaB)
SENA: AU38_11540(arnT)
SENO: AU37_11550(arnT)
SENV: AU39_11550(arnT)
SENQ: AU40_12930(arnT)
SENL: IY59_11865(arnT)
SENJ: CFSAN001992_22070(arnT)
SEEC: CFSAN002050_18430(arnT)
SEEB: SEEB0189_008065(arnT)
SEEP: I137_02715(arnT)
SENB: BN855_23820(pqaB)
SENE: IA1_11460(arnT)
SENC: SEET0819_18595(arnT)
SBG: SBG_2090(pqaB)
SBZ: A464_2415
SBV: N643_10220(arnT)
SFL: SF2336(arnT)
SFX: S2469
SFV: SFV_2327
SFE: SFxv_2579(arnT)
SFN: SFy_3324
SFS: SFyv_3399
SFT: NCTC1_02569(arnT)
SSN: SSON_2318
SBO: SBO_2294
SBC: SbBS512_E2633(arnT)
SDY: SDY_2453(arnT)
SHQ: A0259_15105(arnT)
ENC: ECL_04859
ENO: ECENHK_20875(arnT)
ECLO: ENC_26900
EEC: EcWSU1_04280(arnT)
ENL: A3UG_21610(arnT)
ECLE: ECNIH2_22135(arnT)
ECLN: ECNIH4_01700(arnT)
ECLY: LI62_23400(arnT)
EHM: AB284_03410(arnT)
EAU: DI57_19855(arnT)
ECLS: LI67_022815(arnT)
ENX: NI40_020900(arnT)
ENF: AKI40_0314(arnT)
KPN: KPN_03843(arnT)
KPU: KP1_5180(arnT)
KPP: A79E_0273
KPH: KPNIH24_24910(arnT)
KPZ: KPNIH27_23830(arnT)
KPV: KPNIH29_24750(arnT)
KPW: KPNIH30_25200(arnT)
KPY: KPNIH31_24255(arnT)
KPG: KPNIH32_26070(arnT)
KPC: KPNIH10_24875(arnT)
KPQ: KPR0928_24395(arnT)
KPT: VK055_3627(arnT)
KPE: KPK_0270(arnT)
KPO: KPN2242_22225(arnT)
KPR: KPR_5074(arnT)
KPJ: N559_0311
KPX: PMK1_01345(arnT)
KPB: FH42_15550(arnT)
KPNE: KU54_001395(arnT)
KPNU: LI86_01390(arnT)
KPNK: BN49_0274(arnT)
KVA: Kvar_0258
KPK: A593_09420(arnT)
KOX: KOX_05060(arnT)
KOE: A225_5479
KOY: J415_04700(arnT)
KOM: HR38_03535(arnT)
KMI: VW41_09745(arnT)
KOK: KONIH1_27585(arnT)
KOC: AB185_08795(arnT)
EAE: EAE_05680(arnT)
EAR: CCG32862
CFD: CFNIH1_22850(arnT)
CAMA: F384_12085(arnT)
GQU: AWC35_22985(arnT)
HDE: HDEF_0859(arnT)
SECT: A359_07500
SEHC: A35E_00189
ROR: RORB6_20135(arnT)
RON: TE10_00860(arnT)
CNT: JT31_02760(arnT)
CEM: LH23_15435(arnT)
CEN: LH86_14585(arnT)
PGE: LG71_17155(arnT)
KLE: AO703_20160(arnT)
KOR: AWR26_01325(arnT)
KRD: A3780_23405(arnT)
EBF: D782_1297
YPE: YPO2418(arnT)
YPK: y1921
YPH: YPC_2053(arnT)
YPA: YPA_1762
YPN: YPN_1872
YPM: YP_2205
YPG: YpAngola_A2608(arnT)
YPZ: YPZ3_1469
YPT: A1122_14050(arnT)
YPD: YPD4_1434
YPX: YPD8_1511
YPW: CH59_4252
YPJ: CH55_494
YPV: BZ15_1107
YPL: CH46_2687
YPS: YPTB2326(pmrK)
YPO: BZ17_128
YPI: YpsIP31758_1729(arnT)
YPY: YPK_1835
YPB: YPTS_2401
YPQ: DJ40_4259
YPU: BZ21_1614
YPR: BZ20_3917
YPC: BZ23_1900
YPF: BZ19_1691
YEN: YE2188(arnT)
YEY: Y11_08021
YEW: CH47_1623
YET: CH48_3633
YSI: BF17_20730(arnT)
YAL: AT01_264
YFR: AW19_1394
YIN: CH53_4131
YKR: CH54_268
YRU: BD65_477
YRB: UGYR_02440(arnT)
SPE: Spro_2158
SRL: SOD_c19720(arnT)
SPLY: Q5A_010910(arnT)
SMAF: D781_1989
SMW: SMWW4_v1c21870(arnT)
SMAR: SM39_1619(arnT)
SMAC: SMDB11_1432(arnT)
SERF: L085_17830(arnT)
SERS: SERRSCBI_10255(arnT)
SFW: WN53_19970(arnT)
SFG: AV650_15790(arnT)
SFO: Z042_24145(arnT)
RAA: Q7S_14220(arnT)
ECA: ECA3142
PATR: EV46_15555(arnT)
PATO: GZ59_31520
PCT: PC1_2924
PCV: BCS7_14875(arnT)
PEC: W5S_1260
SOD: Sant_1034(arnT1) Sant_3567(arnT2)
PES: SOPEG_0622(arnT1) SOPEG_3211(arnT2)
DDD: Dda3937_01076(arnT)
DSO: A4U42_09045(arnT)
CED: LH89_12325(arnT)
DDQ: DDI_4293
BGJ: AWC36_13845(arnT)
ETA: ETA_23790(arnT)
EPY: EpC_25140(arnT)
EPR: EPYR_02724(yfbI)
EAM: EAMY_1087(yfbI)
EAY: EAM_1094(arnT)
EBI: EbC_12150(arnT)
ERJ: EJP617_22130(yfbI)
EGE: EM595_p0070(arnT)
WBR: b2257
WGL: WIGMOR_0620(arnT)
PAM: PANA_4006(arnT)
PLF: PANA5342_p10124(arnT)
PAJ: PAJ_p0003(arnT)
PAQ: PAGR_p095
PVA: Pvag_pPag10068(yfbI)
PANT: PSNIH1_p00705(arnT)
PSTW: DSJ_25415
TPTY: NCTC11468_02695(arnT)
PLU: plu2656(pbgE1)
PLUM: A4R40_13225(arnT)
PAY: PAU_01879(arnT)
PTT: VY86_18220(arnT)
PMR: PMI0275(arnT) PMI1047(arnT)
PMIB: BB2000_0425(arnT) BB2000_1084(arnT)
XBO: XBJ1_2532(arnT)
XBV: XBW1_3008(pbgE)
XNE: XNC1_1839(pbgE1)
XNM: XNC2_1784(pbgE)
XDO: XDD1_1783(pbgE)
XPO: XPG1_1550(pbgE)
PSI: S70_08185(arnT) S70_19375(arnT)
PSX: DR96_1542(arnT) DR96_2787
PRG: RB151_004810(arnT_1) RB151_019270(arnT_3)
PHEI: NCTC12003_00479(arnT_1) NCTC12003_02330(arnT_2)
ETR: ETAE_1295(arnT)
ETD: ETAF_1207(pqaB)
ETE: ETEE_3270(arnT)
ETC: ETAC_06140(arnT)
EDW: QY76_13715(arnT)
EDL: AAZ33_06740(arnT)
HAV: AT03_10635(arnT)
OPO: DSM2777_16840(arnT)
PFQ: QQ39_15915(arnT)
KIN: AB182_18770(arnT)
PAE: PA3556(arnT)
PAEV: N297_3679
PAEI: N296_3679
PAU: PA14_18330(arnT)
PAG: PLES_14771(arnT)
PAF: PAM18_1430(arnT)
PNC: NCGM2_4682(arnT)
PAEB: NCGM1900_2727(arnT)
PDK: PADK2_06585(arnT)
PSG: G655_07020(arnT)
PAEP: PA1S_07640
PAEM: U769_07115
PAEL: T223_07355
PAEU: BN889_03927(arnT)
PAEG: AI22_26265
PAEC: M802_3676
PAEO: M801_3544
PCQ: PcP3B5_10950(arnT_1)
PSB: Psyr_2693(arnT)
PSYR: N018_13785
PFL: PFL_3047(arnT_1) PFL_4461(arnT_2)
PPRC: PFLCHA0_c30750(arnT1) PFLCHA0_c45320(arnT2)
PPRO: PPC_3075(arnT_1) PPC_4570(arnT_2)
PFC: PflA506_2686(arnT_1) PflA506_3380(arnT_2)
PPZ: H045_08700(arnT) H045_10940(arnT)
PMAN: OU5_0513
PDR: H681_05030(arnT)
PKC: PKB_2816(arnT2) PKB_4723(arnT2)
PSOS: POS17_3051(arnT_1) POS17_4533(arnT_2)
SSE: Ssed_0922
AHA: AHA_0988(arnT)
AHY: AHML_05280(arnT)
AHD: AI20_14320(arnT)
AHR: V428_05500(arnT)
AHP: V429_05505(arnT)
AHJ: V469_17850(arnT)
AHH: RY45_05445(arnT)
AHI: VU14_17570(arnT)
ASA: ASA_3311(arnT)
ASR: WL1483_1567(arnT)
SALN: SALB1_1042
DEU: DBW_3191
FMR: Fuma_00661(arnT)
GES: VT84_32365(arnT_4)
DORI: FH5T_19895
HSW: Hsw_1557
GFO: GFO_1217
GFL: GRFL_3364
ZPR: ZPR_3258
 » show all
Taxonomy
Reference
1  [PMID:11535604]
  Authors
Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR
  Title
An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants  and role of a novel lipid-linked donor.
  Journal
J Biol Chem 276:43122-31 (2001)
DOI:10.1074/jbc.M106961200
  Sequence
[eco:b2257] [stm:STM2301]
Reference
2  [PMID:11535605]
  Authors
Trent MS, Ribeiro AA, Doerrler WT, Lin S, Cotter RJ, Raetz CR
  Title
Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm.
  Journal
J Biol Chem 276:43132-44 (2001)
DOI:10.1074/jbc.M106962200
Reference
3  [PMID:11535603]
  Authors
Zhou Z, Ribeiro AA, Lin S, Cotter RJ, Miller SI, Raetz CR
  Title
Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation.
  Journal
J Biol Chem 276:43111-21 (2001)
DOI:10.1074/jbc.M106960200
Reference
4  [PMID:16226890]
  Authors
Bretscher LE, Morrell MT, Funk AL, Klug CS
  Title
Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium.
  Journal
Protein Expr Purif 46:33-9 (2006)
DOI:10.1016/j.pep.2005.08.028
  Sequence
[stm:STM2301]
Reference
5  [PMID:19947657]
  Authors
Impellitteri NA, Merten JA, Bretscher LE, Klug CS
  Title
Identification of a functionally important loop in Salmonella typhimurium ArnT.
  Journal
Biochemistry 49:29-35 (2010)
DOI:10.1021/bi901572f
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.43
IUBMB Enzyme Nomenclature: 2.4.2.43
ExPASy - ENZYME nomenclature database: 2.4.2.43
BRENDA, the Enzyme Database: 2.4.2.43

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