KEGG   ENZYME: 2.4.99.18Help
Entry
EC 2.4.99.18                Enzyme                                 

Name
dolichyl-diphosphooligosaccharide---protein glycotransferase;
dolichyldiphosphooligosaccharide-protein glycosyltransferase;
asparagine N-glycosyltransferase;
dolichyldiphosphooligosaccharide-protein oligosaccharyltransferase;
dolichylpyrophosphodiacetylchitobiose-protein glycosyltransferase;
oligomannosyltransferase;
oligosaccharide transferase;
dolichyldiphosphoryloligosaccharide-protein oligosaccharyltransferase;
dolichyl-diphosphooligosaccharide:protein-L-asparagine oligopolysaccharidotransferase;
STT3
Class
Transferases;
Glycosyltransferases;
Transferring other glycosyl groups
BRITE hierarchy
Sysname
dolichyl-diphosphooligosaccharide:protein-L-asparagine N-beta-D-oligopolysaccharidotransferase
Reaction(IUBMB)
dolichyl diphosphooligosaccharide + [protein]-L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine [RN:R04216]
Reaction(KEGG)
Substrate
dolichyl diphosphooligosaccharide [CPD:C04213];
[protein]-L-asparagine
Product
dolichyl diphosphate [CPD:C00621];
glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
Comment
Occurs in eukaryotes that form a glycoprotein by the transfer of a glucosyl-mannosyl-glucosamine polysaccharide to the side-chain of an L-asparagine residue in the sequence -Asn-Xaa-Ser- or -Asn-Xaa-Thr- (Xaa not Pro) in nascent polypeptide chains. The basic oligosaccharide is the tetradecasaccharide Glc3Man9GlcNAc2 (for diagram {polysacc/Dol14}). However, smaller oligosaccharides derived from it and oligosaccharides with additional monosaccharide units attached may be involved. See ref [2] for a review of N-glycoproteins in eukaryotes. Man3GlcNAc2 seems to be common for all of the oligosaccharides involved with the terminal N-acetylglucosamine linked to the protein L-asparagine. Occurs on the cytosolic face of the endoplasmic reticulum. The dolichol involved normally has 14-21 isoprenoid units with two trans double-bonds at the omega end, and the rest of the double-bonds in cis form.
History
EC 2.4.99.18 created 1984 as EC 2.4.1.119, transferred 2012 to EC 2.4.99.18
Pathway
ec00510  N-Glycan biosynthesis
ec00513  Various types of N-glycan biosynthesis
ec01100  Metabolic pathways
Orthology
K07151  dolichyl-diphosphooligosaccharide---protein glycosyltransferase
Genes
HSA: 201595(STT3B) 3703(STT3A)
PTR: 451648(STT3A) 746396(STT3B)
PPS: 100971755(STT3B) 100981439(STT3A)
GGO: 101133351(STT3A) 101149109(STT3B)
PON: 100174363(STT3A) 100457422(STT3B)
NLE: 100596112(STT3A) 100607757(STT3B)
MCC: 703434(STT3B) 717056(STT3A)
MCF: 101864904(STT3B) 102135340(STT3A)
CSAB: 103241813(STT3B) 103248767(STT3A)
RRO: 104653935(STT3A) 104655080(STT3B)
RBB: 108516559(STT3B) 108542335(STT3A)
CJC: 100390946(STT3B) 100404567(STT3A)
SBQ: 101043918(STT3A) 101045399(STT3B)
MMU: 16430(Stt3a) 68292(Stt3b)
RNO: 363160(Stt3b) 500972(Stt3a)
CGE: 100751391(Stt3a) 100752084(Stt3b)
NGI: 103731954(Stt3b) 103735593(Stt3a)
HGL: 101700446(Stt3a) 101708251(Stt3b)
CCAN: 109697850(Stt3b) 109700304(Stt3a)
OCU: 100337742(STT3B) 100348537(STT3A)
TUP: 102489695(STT3B) 102500219(STT3A)
CFA: 485628(STT3B) 489300(STT3A)
AML: 100467601(STT3A) 100470123(STT3B)
UMR: 103660628(STT3A) 103680114(STT3B)
ORO: 101363111(STT3A) 101377932(STT3B)
FCA: 101083108(STT3A) 101092883(STT3B)
PTG: 102949644(STT3A) 102952968(STT3B)
AJU: 106976642(STT3A) 106984259(STT3B)
BTA: 504474(STT3B) 507815(STT3A)
BOM: 102267585(STT3B) 102278791(STT3A)
BIU: 109554454(STT3A) 109576183(STT3B)
PHD: 102319268(STT3B) 102340277(STT3A)
CHX: 102176834(STT3B) 102184597(STT3A)
OAS: 101110978(STT3A) 101111133(STT3B)
SSC: 100511525(STT3A) 100526009(STT3B)
CFR: 102506178(STT3A) 102516239(STT3B)
CDK: 105087201(STT3B) 105094455(STT3A)
BACU: 103004943(STT3B) 103016256(STT3A)
LVE: 103073153(STT3A) 103073836(STT3B)
OOR: 101269582(STT3A) 101289840(STT3B)
ECB: 100057241(STT3B) 100072333(STT3A)
EPZ: 103557007(STT3A) 103561989(STT3B)
EAI: 106830999(STT3B) 106835961(STT3A)
MYB: 102256403(STT3A) 102262523(STT3B)
MYD: 102753759(STT3B) 102765077(STT3A)
HAI: 109391402(STT3A) 109394442(STT3B)
PALE: 102882648(STT3A) 102889868(STT3B)
LAV: 100658795(STT3A) 100667133(STT3B)
MDO: 100016135(STT3A) 100617492(STT3B)
SHR: 100914144(STT3B) 100933132(STT3A)
OAA: 100077452(STT3B) 100090390(STT3A)
GGA: 100857165(STT3A) 428444(STT3B)
MGP: 100544158(STT3A) 100547170(STT3B)
CJO: 107309263(STT3B) 107324223(STT3A)
APLA: 101790992(STT3A) 101794604(STT3B)
ACYG: 106040606(STT3A) 106043315(STT3B)
TGU: 100225468(STT3B) 100230089(STT3A)
GFR: 102035202(STT3A) 102040723(STT3B)
FAB: 101810403(STT3B) 101815558(STT3A)
PHI: 102103326(STT3A) 102112167(STT3B)
PMAJ: 107199976(STT3B) 107214486(STT3A)
CCW: 104689039(STT3B) 104692142(STT3A)
FPG: 101914297(STT3B) 101924303(STT3A)
FCH: 102053953(STT3B) 102056036(STT3A)
CLV: 102084919(STT3A) 102088224(STT3B)
EGZ: 104122959(STT3B) 104129202(STT3A)
AAM: 106491217(STT3B) 106498504(STT3A)
ASN: 102381490(STT3B) 102383937(STT3A)
AMJ: 102572644(STT3B) 106736773(STT3A)
PSS: 102443860(STT3B) 102450612(STT3A)
CMY: 102932718(STT3B) 102945433(STT3A)
CPIC: 101933162(STT3B) 101933331(STT3A)
ACS: 100552304(stt3b) 100565006(stt3a)
PVT: 110070288(STT3A) 110082162(STT3B)
PBI: 103049912(STT3B) 103064352(STT3A)
GJA: 107116491(STT3B) 107117800(STT3A)
XLA: 100380973(stt3b.S) 108718938(stt3b.L) 399234(stt3a.L) 779022(stt3a.S)
XTR: 100495934(stt3b) 407861(stt3a)
NPR: 108783837(STT3A) 108792879(STT3B)
DRE: 266797(stt3a) 323918(stt3b)
IPU: 108270247(stt3b) 108276860(stt3a)
TRU: 101073975(stt3b) 101076802 101077895(stt3a)
LCO: 104922533 104932568(stt3b) 104933910(stt3a)
LCF: 108889079 108893455(stt3a)
HCQ: 109516367(stt3b) 109531141(stt3a)
ELS: 105008063 105010974(stt3a) 105023487(stt3b)
SFM: 108932108(stt3a) 108942511(stt3b)
LCM: 102353150(STT3B) 102360886(STT3A)
CMK: 103176264(stt3b) 103180290(stt3a)
DME: Dmel_CG1518(Stt3A) Dmel_CG7748(Stt3B)
DSI: Dsimw501_GD18237(Dsim_GD18237) Dsimw501_GD24643(Dsim_GD24643)
AME: 409265 551853(STT3B)
PXY: 105380797
CEL: CELE_T12A2.2(stt-3)
CBR: CBG17485
HMG: 100202029 100205648(stt3b)
ATH: AT1G34130(STT3B) AT5G19690(STT3A)
LJA: Lj2g3v0609780.1(Lj2g3v0609780.1) Lj6g3v2156550.1(Lj6g3v2156550.1)
VVI: 100232931(LTM1) 100261650
DOSA: Os04t0675500-01(Os04g0675500) Os05t0519900-01(Os05g0519900)
ATS: 109736166(LOC109736166) 109741062(LOC109741062) 109758378(LOC109758378)
APRO: F751_0618
SCE: YGL022W(STT3)
ERC: Ecym_1435
KMX: KLMA_30378(STT3)
NCS: NCAS_0A01720(NCAS0A01720)
NDI: NDAI_0D03640(NDAI0D03640)
TPF: TPHA_0K01660(TPHA0K01660)
TBL: TBLA_0A09010(TBLA0A09010)
TDL: TDEL_0F02490(TDEL0F02490)
KAF: KAFR_0F03180(KAFR0F03180)
PIC: PICST_82607(STT3)
CAL: CAALFM_C201670CA(STT3)
CAUR: QG37_02673
SLB: AWJ20_3455(STT3)
NCR: NCU10497(ti)
NTE: NEUTE1DRAFT150374(NEUTE1DRAFT_150374)
MGR: MGG_04773
SSCK: SPSK_00705
MAW: MAC_03175
MAJ: MAA_04270
CMT: CCM_06368
BFU: BCIN_02g02240(Bcstt3)
MBE: MBM_00171
ANI: AN1455.2
ANG: ANI_1_1150144(An16g08570)
ABE: ARB_06492
TVE: TRV_00571
PTE: PTT_06248
SPO: SPBC1271.02(stt3)
CNE: CNI01850
CNB: CNBH1740
LBC: LACBIDRAFT_305018(STT3)
ABP: AGABI1DRAFT111863(AGABI1DRAFT_111863)
ABV: AGABI2DRAFT193103(AGABI2DRAFT_193103)
MGL: MGL_3807
DDI: DDB_G0285159(stt3)
DFA: DFA_01189(stt3)
EHI: EHI_011940(16.t00004) EHI_118880(62.t00006)
PYO: PY17X_0933400(PY02151)
PCB: PCHAS_091290(PC000272.05.0)
BBO: BBOV_II000220(18.m05999)
CPV: cgd6_2040
SMIN: v1.2.011374.t1(symbB.v1.2.011374.t1) v1.2.013974.t1(symbB.v1.2.013974.t1) v1.2.021176.t1(symbB.v1.2.021176.t1) v1.2.022879.t1(symbB.v1.2.022879.t1) v1.2.034733.t1(symbB.v1.2.034733.t1) v1.2.036276.t1(symbB.v1.2.036276.t1) v1.2.036276.t2(symbB.v1.2.036276.t2) v1.2.038098.t1(symbB.v1.2.038098.t1)
TCR: 505163.80
NHL: Nhal_0376
WSU: WS0043(WLAF)
SDL: Sdel_1767
NIS: NIS_1250
SUN: SUN_0103
NAM: NAMH_1652
DVU: DVU1252
DVL: Dvul_1810
DVM: DvMF_0846
DDE: Dde_3699
DDS: Ddes_0746
DGG: DGI_2311
DAS: Daes_2981
DPI: BN4_12632
DBA: Dbac_3265
DRT: Dret_0285
ADE: Adeh_2819
TTK: TST_1762(pglB)
CTHI: THC_0639
METE: tca_00386(pglB_1) tca_01834 tca_01844(pglB_3)
MFV: Mfer_0623
FPL: Ferp_2437
GAC: GACE_1401
GAH: GAH_01455
TAC: Ta1136
TVO: TVG1271204(TVG1271204)
FAI: FAD_0235
CDIV: CPM_1322
ABI: Aboo_0310
PHO: PH0242(PH0242) PH1271(PH1271)
MBU: Mbur_1579
MMET: MCMEM_2091
MMH: Mmah_0123
MPY: Mpsy_2355
MLA: Mlab_0662
MEMA: MMAB1_1829
MPI: Mpet_0084
MPL: Mpal_0785
HAL: VNG_0005H VNG_1068G(tot)
HSL: OE_1008F OE_2548F(aglB)
HHB: Hhub_1167 Hhub_2271(aglB)
HALH: HTSR_0845(aglB)
HHSR: HSR6_0872(aglB)
HMA: rrnAC0431(tot) rrnAC3242
HHI: HAH_0492 HAH_1202(stt3)
NPH: NP_3720A(aglB)
NMO: Nmlp_2363(aglB)
HWA: HQ_2681A(aglB)
HWC: Hqrw_3013(aglB) Hqrw_3360
HVO: HVO_1530(aglB)
HME: HFX_1592(stt3)
HLA: Hlac_1062
HTU: Htur_2957
NMG: Nmag_0090 Nmag_0927(aglB)
SALI: L593_04990
SMR: Smar_0223
IHO: Igni_0016
IIS: EYM_04420
DKA: DKAM_0136
TAG: Tagg_0313
IAG: Igag_0094
HBU: Hbut_1205
STO: STK_09400
SSO: SSO1052
SOL: Ssol_2025
SSOA: SULA_2057
SSOL: SULB_2058
SSOF: SULC_2056
SAI: Saci_1274
SID: M164_1156
SII: LD85_1283
SIH: SiH_1127
SIR: SiRe_1041
SIC: SiL_1050
MSE: Msed_1805
MCN: Mcup_0430
AHO: Ahos_1254
PAI: PAE3030
PIS: Pisl_0431
PCL: Pcal_0997
PAS: Pars_1781
PYR: P186_1486
POG: Pogu_0350
TNE: Tneu_1689
CMA: Cmaq_0438
TUZ: TUZN_0151
TTN: TTX_0519
VDI: Vdis_2064
VMO: VMUT_0472
TPE: Tpen_0640
NMR: Nmar_0075
NCT: NMSP_0121
NEV: NTE_03038
TAA: NMY3_00408(ppiB_1)
NBV: T478_1421
NDV: NDEV_0103
NEQ: NEQ155
KCR: Kcr_1056
BARC: AOA65_2311
BARB: AOA66_0636
 » show all
Taxonomy
Reference
1  [PMID:6933437]
  Authors
Das RC, Heath EC.
  Title
Dolichyldiphosphoryloligosaccharide--protein oligosaccharyltransferase; solubilization, purification, and properties.
  Journal
Proc Natl Acad Sci U S A 77:3811-5 (1980)
DOI:10.1073/pnas.77.7.3811
Reference
2  [PMID:21605711]
  Authors
Song W, Henquet MG, Mentink RA, van Dijk AJ, Cordewener JH, Bosch D, America AH, van der Krol AR
  Title
N-glycoproteomics in plants: perspectives and challenges.
  Journal
J Proteomics 74:1463-74 (2011)
DOI:10.1016/j.jprot.2011.05.007
Other DBs
ExplorEnz - The Enzyme Database: 2.4.99.18
IUBMB Enzyme Nomenclature: 2.4.99.18
ExPASy - ENZYME nomenclature database: 2.4.99.18
BRENDA, the Enzyme Database: 2.4.99.18
CAS: 75302-32-8

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