KEGG   ENZYME: 2.4.99.19Help
Entry
EC 2.4.99.19                Enzyme                                 

Name
undecaprenyl-diphosphooligosaccharide---protein glycotransferase;
PglB
Class
Transferases;
Glycosyltransferases;
Transferring other glycosyl groups
BRITE hierarchy
Sysname
tritrans,heptacis-undecaprenyl-diphosphooligosaccharide:protein-L-asparagine N-beta-D-oligosaccharidotransferase
Reaction(IUBMB)
tritrans,heptacis-undecaprenyl diphosphooligosaccharide + [protein]-L-asparagine = tritrans,heptacis-undecaprenyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine
Substrate
tritrans,heptacis-undecaprenyl diphosphooligosaccharide;
[protein]-L-asparagine
Product
tritrans,heptacis-undecaprenyl diphosphate [CPD:C19852];
glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to protein L-asparagine
Comment
A bacterial enzyme that has been isolated from Campylobacter jejuni [1] and Campylobacter lari [2]. It forms a glycoprotein by the transfer of a glucosyl-N-acetylgalactosaminyl-N,N'-diacetylbacillosamine (GalNAc2(Glc)GalNAc3diNAcBac) polysaccharide and related oligosaccharides to the side-chain of an L-asparagine residue in the sequence -Asp/Glu-Xaa-Asn-Xaa'-Ser/Thr- (Xaa and Xaa' not Pro) in nascent polypeptide chains. Requires Mn2+ or Mg2+. Occurs on the external face of the plasma membrane. The polyprenol involved is normally tritrans,heptacis-undecaprenol but a decaprenol is used by some species.
History
EC 2.4.99.19 created 2012
Orthology
K17251  undecaprenyl-diphosphooligosaccharide---protein glycotransferase
Genes
SUA: Saut_1513
SULR: B649_10060
CJE: Cj1126c(pglB)
CJB: BN148_1126c(pglB)
CJJ: CJJ81176_1143(pglB)
CJU: C8J_1066(wlaF)
CJN: ICDCCJ07001_1082 ICDCCJ07001_1083
CJI: CJSA_1068(pglB)
CJM: CJM1_1104(wlaF)
CJS: CJS3_1172(pglB)
CJEJ: N564_01094
CJEU: N565_01137
CJEN: N755_01130
CJEI: N135_01163
CJER: H730_06600
CJV: MTVDSCj20_1129(pglB)
CJY: QZ67_01203(pglB_1) QZ67_01204(pglB_2)
CJQ: UC78_1083(pglB)
CJR: CJE1268(pglB)
CJD: JJD26997_0595(pglB)
CFT: CFF04554_1398(pglB)
CFV: CFVI03293_1421(pglB)
CFX: CFV97608_1521(pglB)
CFZ: CSG_15200
CAMP: CFT03427_1346(pglB)
CCV: CCV52592_1213(pglB)
CCO: CCC13826_0452(pglB)
CLA: Cla_1253(pglB)
CLR: UPTC16701_1236(pglB)
CLM: UPTC16712_1254(pglB)
CLQ: UPTC4110_1238(pglB)
CLN: UPTC3659_1473(pglB)
CLL: CONCH_1204(pglB)
CCQ: N149_1067
CCF: YSQ_03340
CCY: YSS_06095
CCOI: YSU_03375
CCOF: VC76_05525(pglB)
CCOO: ATE51_01352(pglB)
CAJ: CIG1485E_1392(pglB)
CIS: CINS_1218(pglB)
CVO: CVOL_1232(pglB)
CPEL: CPEL_1359(pglB)
CAMR: CAQ16704_1264(pglB)
CSM: CSUB8521_1440(pglB)
CSF: CSUB8523_1536(pglB)
CGRA: CGRAC_1490(pglB) CGRAC_1892
CURE: CUREO_1374(pglB)
CHYO: CHH_0377(pglB)
CHV: CHELV3228_0554(pglB)
CSPF: CSF_0893(pglB)
CPIN: CPIN18020_0862(pglB)
CCUN: CCUN_1171(pglB)
CLX: CLAN_0354(pglB)
CAVI: CAV_0416(pglB)
SMUL: SMUL_2464
SHAL: SHALO_2210
SULJ: SJPD1_2224
 » show all
Taxonomy
Reference
1  [PMID:20007322]
  Authors
Maita N, Nyirenda J, Igura M, Kamishikiryo J, Kohda D
  Title
Comparative structural biology of eubacterial and archaeal oligosaccharyltransferases.
  Journal
J Biol Chem 285:4941-50 (2010)
DOI:10.1074/jbc.M109.081752
  Sequence
[cjr:CJE1268]
Reference
2  [PMID:21677752]
  Authors
Lizak C, Gerber S, Numao S, Aebi M, Locher KP
  Title
X-ray structure of a bacterial oligosaccharyltransferase.
  Journal
Nature 474:350-5 (2011)
DOI:10.1038/nature10151
  Sequence
[cla:Cla_1253]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.99.19
IUBMB Enzyme Nomenclature: 2.4.99.19
ExPASy - ENZYME nomenclature database: 2.4.99.19
BRENDA, the Enzyme Database: 2.4.99.19

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