KEGG   ENZYME: 2.4.99.6Help
Entry
EC 2.4.99.6                 Enzyme                                 

Name
N-acetyllactosaminide alpha-2,3-sialyltransferase;
cytidine monophosphoacetylneuraminate-beta-galactosyl(1->4)acetylglucosaminide alpha2->3-sialyltransferase;
alpha2->3 sialyltransferase (ambiguous);
SiaT (ambiguous);
CMP-N-acetylneuraminate:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-glycoprotein alpha-2,3-N-acetylneuraminyltransferase;
neolactotetraosylceramide alpha-2,3-sialyltransferase;
CMP-N-acetylneuraminate:beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein alpha-(2->3)-N-acetylneuraminyltransferase
Class
Transferases;
Glycosyltransferases;
Transferring other glycosyl groups
BRITE hierarchy
Sysname
CMP-N-acetyl-beta-neuraminate:beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R (2->3)-N-acetyl-alpha-neuraminyltransferase (configuration-inverting)
Reaction(IUBMB)
CMP-N-acetyl-beta-neuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = CMP + N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R [RN:R04596 R09297]
Reaction(KEGG)
R04596 R09297(G);
(other) R04656 R06026(G) R06037(G) R06153(G)
Show
Substrate
CMP-N-acetyl-beta-neuraminate [CPD:C00128];
beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R [CPD:C00694]
Product
CMP [CPD:C00055];
N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R
Comment
The enzyme recognizes the sequence beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl (known as type 2 histo-blood group precursor disaccharide) in non-reducing termini of glycan moieties in glycoproteins and glycolipids. The enzyme from chicken brain was shown to act on neolactotetraosylceramide, producing ganglioside LM1 [2].
History
EC 2.4.99.6 created 1984, modified 1986 (EC 2.4.99.10 created 1986, incorporated 2017)
Pathway
ec00513  Various types of N-glycan biosynthesis
ec00514  Other types of O-glycan biosynthesis
ec00515  Mannose type O-glycan biosynthesis
ec00533  Glycosaminoglycan biosynthesis - keratan sulfate
ec00601  Glycosphingolipid biosynthesis - lacto and neolacto series
ec01100  Metabolic pathways
Orthology
K00781  neolactotetraosylceramide alpha-2,3-sialyltransferase (sialyltransferase 6)
K03792  neolactotetraosylceramide alpha-2,3-sialyltransferase (sialyltransferase 10)
Genes
HSA: 10402(ST3GAL6) 6487(ST3GAL3)
PTR: 456832(ST3GAL3) 460547(ST3GAL6)
PPS: 100977718(ST3GAL6) 100982060(ST3GAL3)
GGO: 101126091(ST3GAL3) 101133620(ST3GAL6)
PON: 100171770(ST3GAL6) 100441500(ST3GAL3)
NLE: 100583585(ST3GAL6) 100596717(ST3GAL3)
MCC: 699096(ST3GAL6) 703599(ST3GAL3)
MCF: 102115716(ST3GAL6) 102124772(ST3GAL3)
CSAB: 103224957(ST3GAL3) 103228470(ST3GAL6)
RRO: 104654524(ST3GAL6) 104669039(ST3GAL3)
RBB: 108516678(ST3GAL6) 108535692(ST3GAL3)
CJC: 100389235(ST3GAL6) 100411797(ST3GAL3)
SBQ: 101030408 101041573(ST3GAL3)
MMU: 20441(St3gal3) 54613(St3gal6)
RNO: 304023(St3gal6) 64445(St3gal3)
CGE: 100689187(St3gal3) 100771326(St3gal6)
NGI: 103732633(St3gal6) 103743864(St3gal3)
HGL: 101706310(St3gal6) 101716398(St3gal3)
CCAN: 109681562 109681564(St3gal3) 109696030(St3gal6)
OCU: 100339165(ST3GAL6) 100353106(ST3GAL3)
TUP: 102481523(ST3GAL6) 102486753(ST3GAL3)
CFA: 478535(ST3GAL6) 610736(ST3GAL3)
AML: 100472631(ST3GAL3) 100481119(ST3GAL6)
UMR: 103670807(ST3GAL3) 103673244(ST3GAL6)
ORO: 101366681(ST3GAL3) 101383996(ST3GAL6)
FCA: 101081911(ST3GAL3) 101091552(ST3GAL6)
PTG: 102952023(ST3GAL6) 102966623(ST3GAL3)
AJU: 106970220(ST3GAL3) 106982524(ST3GAL6)
BTA: 444859(ST3GAL3) 444860(ST3GAL6)
BOM: 102265994(ST3GAL6) 102284552(ST3GAL3)
BIU: 109556395(ST3GAL3) 109564929(ST3GAL6)
PHD: 102337048(ST3GAL6) 102343639(ST3GAL3)
CHX: 102168794(ST3GAL3) 102175162(ST3GAL6)
OAS: 101106812(ST3GAL6) 101112269(ST3GAL3)
SSC: 100739492(ST3GAL6) 396619(ST3GAL3)
CFR: 102516778(ST3GAL6) 102517093(ST3GAL3)
CDK: 105087739(ST3GAL6) 105090902(ST3GAL3)
BACU: 103001584(ST3GAL6) 103003034(ST3GAL3)
LVE: 103078993(ST3GAL6) 103083644(ST3GAL3)
OOR: 101286804(ST3GAL6) 101287105(ST3GAL3)
ECB: 100053104(ST3GAL3) 100062218(ST3GAL6)
EPZ: 103561946(ST3GAL6) 103567339
EAI: 106824252(ST3GAL6) 106847915(ST3GAL3)
MYB: 102245915(ST3GAL3) 102260204(ST3GAL6)
MYD: 102754272(ST3GAL6) 102773348(ST3GAL3)
HAI: 109377051(ST3GAL6) 109383404(ST3GAL3)
RSS: 109457885(ST3GAL3) 109460793(ST3GAL6)
PALE: 102883140(ST3GAL3) 102889744(ST3GAL6)
LAV: 100668469(ST3GAL3) 100672752(ST3GAL6)
MDO: 100017812(ST3GAL6) 100025401(ST3GAL3)
SHR: 100917951(ST3GAL3) 100923762(ST3GAL6)
OAA: 100078221(ST3GAL3) 100082119
GGA: 395138(ST3GAL6) 424572(ST3GAL3)
MGP: 100541741(ST3GAL6) 100543009(ST3GAL3)
CJO: 107317545(ST3GAL3) 107320596(ST3GAL6)
APLA: 101802079(ST3GAL6) 101804175(ST3GAL3)
ACYG: 106033989(ST3GAL3) 106038338(ST3GAL6)
TGU: 100221242(ST3GAL3) 100222736(ST3GAL6)
GFR: 102034732(ST3GAL3) 102043783(ST3GAL6)
FAB: 101807588(ST3GAL3) 101815960(ST3GAL6)
PHI: 102099818(ST3GAL6) 102104267(ST3GAL3)
PMAJ: 107208043(ST3GAL3) 107210567(ST3GAL6)
CCW: 104687090(ST3GAL6) 104695266(ST3GAL3)
FPG: 101915399(ST3GAL6) 101917466(ST3GAL3)
FCH: 102055195(ST3GAL3) 102060181(ST3GAL6)
CLV: 102083691(ST3GAL6) 102089431(ST3GAL3)
EGZ: 104125511(ST3GAL3) 104126021(ST3GAL6)
AAM: 106484925(ST3GAL3) 106490642(ST3GAL6)
ASN: 102377903(ST3GAL6) 102387225(ST3GAL3)
AMJ: 102570018(ST3GAL6) 102570899(ST3GAL3)
PSS: 102458402(ST3GAL3) 102462369(ST3GAL6)
CMY: 102937505(ST3GAL3) 102947450(ST3GAL6)
CPIC: 101932642(ST3GAL6) 101943704(ST3GAL3)
ACS: 100556902(st3gal3) 100557291(st3gal6)
PVT: 110073793(ST3GAL3) 110074621(ST3GAL6)
PBI: 103048808(ST3GAL3) 103051166(ST3GAL6)
GJA: 107110020(ST3GAL6) 107120284(ST3GAL3)
XLA: 100462908(st3gal-iii) 108709199 108715547(st3Gal-III) 398745(st3gal6.L) 398746(st3gal3.S)
XTR: 402789(st3gal3) 402790(st3gal6)
DRE: 407659(st3gal3b)
IPU: 108271839(st3gal3) 108280530
AMEX: 103026988(st3gal3) 103044556
TRU: 445973(st3gal3)
LCO: 104920950(st3gal3) 104940288
NCC: 104948573 104964780(st3gal3)
MZE: 101484929(st3gal3)
OLA: 100049527(st3gal3)
XMA: 102237009(st3gal3)
PRET: 103479140(st3gal3)
NFU: 107397021(st3gal3)
CSEM: 103395857 103399833(st3gal3)
HCQ: 109518900(st3gal3)
BPEC: 110174824(st3gal3)
SFM: 108923813(st3gal3)
LCM: 102351151(ST3GAL3) 102356485(ST3GAL6)
CMK: 103174609(st3gal3) 103180110(st3gal6)
APLC: 110982962
 » show all
Taxonomy
Reference
1  [PMID:7204397]
  Authors
Van den Eijnden DH, Schiphorst WE.
  Title
Detection of beta-galactosyl(1 leads to 4)N-acetylglucosaminide alpha(2 leads to 3)-sialyltransferase activity in fetal calf liver and other tissues.
  Journal
J Biol Chem 256:3159-62 (1981)
Reference
2  [PMID:7130178]
  Authors
Basu M, Basu S, Stoffyn A, Stoffyn P.
  Title
Biosynthesis in vitro of sialyl(alpha 2-3)neolactotetraosylceramide by a sialyltransferase from embryonic chicken brain.
  Journal
J Biol Chem 257:12765-9 (1982)
Other DBs
ExplorEnz - The Enzyme Database: 2.4.99.6
IUBMB Enzyme Nomenclature: 2.4.99.6
ExPASy - ENZYME nomenclature database: 2.4.99.6
BRENDA, the Enzyme Database: 2.4.99.6
CAS: 77537-85-0

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