KEGG ENZYME: 2.5.1.101

ENZYME: 2.5.1.101

Entry

EC 2.5.1.101                Enzyme

Name

N,N'-diacetyllegionaminate synthase;
neuB (gene name);
legI (gene name)

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

phosphoenolpyruvate:2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose 1-(2-carboxy-2-oxoethyl)transferase

Reaction(IUBMB)

2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O = N,N'-diacetyllegionaminate + phosphate [RN:R10304]

Reaction(KEGG)

R10304

Substrate

2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose [CPD:C20424];
phosphoenolpyruvate [CPD:C00074];
H2O [CPD:C00001]

Product

N,N'-diacetyllegionaminate [CPD:C20418];
phosphate [CPD:C00009]

Comment

Requires a divalent metal such as Mn2+. Isolated from the bacteria Legionella pneumophila and Campylobacter jejuni, where it is involved in the biosynthesis of legionaminic acid, a virulence-associated, cell surface sialic acid-like derivative.

History

EC 2.5.1.101 created 2012

Pathway

ec00520

Amino sugar and nucleotide sugar metabolism

ec01100

Metabolic pathways

Orthology

K18430

N,N'-diacetyllegionaminate synthase

Genes

CITZ:

E4Z61_12350(neuB)

PMAI:

CF386_03205(neuB)

PTW:	TUM18999_39430

PSOA:

PSm6_37510(neuB)

MLQ:	ASQ50_04385

MARJ:

MARI_04460(legI)

MSAN:

LPB19_15080(neuB)

SSPA:

K0I31_07170

COZ:	A3Q34_06165

PTU:	PTUN_a3149(neuB)

ASP:	AOR13_2481

ASQ:	AVL57_11080

AAW:	AVL56_10220

ALE:	AV939_10100

ALZ:	AV940_09870

PMAW:

MACH26_32710

TTU:	TERTU_1310

LPN:	lpg0752(neuB)

LPH:	LPV_0878(neuB)

LPO:	LPO_0833(neuB)

LPU:	LPE509_02459

LPM:	LP6_0732(neuB)

LPF:	lpl0789(neuB)

LPP:	lpp0818(neuB)

LPC:	LPC_2540(neuB)

LPA:

lpa_01160

LPE:

lp12_0762

LCD:	clem_09455(neuB_2)

LWA:	SAMEA4504053_1066(spsE)

LANT:

TUM19329_12800(neuB)

LCAD:

PXX05_08225(neuB)

MEIY:

MIN45_P1358

MCAU:

MIT9_P1308

FPH:

Fphi_1167

FPI:	BF30_1411(neuB)

FPX:	KU46_147(neuB)

FHA:	CDV26_09130(neuB)

FRC:	KX01_1747(neuB)

TCX:

Tcr_1458

HTR:	EPV75_07835(neuB)

HMAR:

HVMH_1511(spsE)

TAO:	THIAE_07350

THIO:

AYJ59_06075

THIG:

FE785_07450(neuB)

TXA:	HQN79_08330(neuB)

TLH:	NR989_02480

TSE:	THMIRHAS_08930(spsE)

TANI:

J8380_09295(neuB)

TEE:

Tel_08595

TBN:

TBH_C0740

EPS:	L0Y14_02440 L0Y14_02510

HAHE:

ENC22_10850

OME:	OLMES_5580

AALL:

I6G90_07895

ARIV:

KYK33_12495

SUTR:

L0B52_04055(neuB)

THIN:

CRN91_03145(neuB) CRN91_03215

THO:	SP60_02330

DEE:	HQN60_14860 HQN60_14875

CARI:

FNU76_04765(neuB)

TVL:	FAZ95_31075(neuB)

PIG:	EGT29_04955

RHOB:

HTY51_14425(neuB)

POS:	DT070_03545(neuB)

RTEA:

HK414_20340(neuB)

PAQA:

K9V56_020865(neuB)

JAJ:	EKL02_02185(neuB)

TIN:

Tint_2652

THI:

THI_3181

METR:

BSY238_2009(neuB)

ARES:

IWH25_01425(neuB)

DECH:

GBK02_12550(neuB)

ACOM:

CEW83_19880(neuB)

EMX:	FKV68_30710(neuB)

RHT:	NT26_3141(neuB)

TALZ:

RPMA_09945

DEI:	C4375_03855(neuB)

DEA:	FPZ08_13230(neuB)

DOY:	JI749_13415(neuB)

DRH:	JI748_11510(neuB)

MHEY:

H2LOC_005785

RBM:

TEF_17315

BSB:	Bresu_0507

RPON:

G3256_17745

RPEL:

N7U68_00670

RMAI:

MACH21_19430

FYT:	QF092_14210(neuB)

BOO:	E2K80_11695

PAED:

G5B38_20080

AMYL:

QBD29_01545

FAQ:	G5B39_16690(neuB)

MMR:	Mmar10_1974

SLAN:

GV829_10185(neuB)

PMAS:

NCF86_12570

ALI:	AZOLI_p60097

MAGX:

XM1_0956

TTB:	MACH01_31150

MAGQ:

MGMAQ_1137

NACI:

NUH88_13130(neuB)

MGM:

Mmc1_0562

PEL:	SAR11G3_00118

HCP:

HCN_1148

HCB:	HCBAA847_0824

TDN:	Suden_0595

SPAL:

FM071_00635(neuB)

SSEI:

FJR45_00975(neuB) FJR45_04420(neuB)

SMAS:

HUE87_09365(neuB)

SMAX:

FJR03_03345(neuB)

SAQT:

GJV85_00945(neuB)

SULR:

B649_08560

CJE:	Cj1327(neuB2)

CJB:	BN148_1327(neuB2)

CJI:	CJSA_1263(neuB2)

CJS:

CJS3_1421

CJP:	A911_06450

CJEJ:

N564_02255

CJEU:

N565_02345

CJEN:

N755_02405

CJEI:

N135_01415

CJER:

H730_07590

CJY:	QZ67_01463(legI_2)

CJQ:	UC78_1270(legI)

CJL:	PJ17_07020

CJW:	PJ18_06820

CJR:	CJE1516(ptmC)

CJD:	JJD26997_0385(ptmC)

CJZ:	M635_02330

CJX:	BN867_13120

CCO:	CCC13826_2303(legI)

CCOC:

CCON33237_1630(legI)

CLA:	CLA_1235(legI)

CLL:	CONCH_0304

CCOL:

BN865_08100c

CCC:	G157_02185

CCQ:	N149_1290(legI)

CCF:

YSQ_02215

CCY:

YSS_07200

CCOI:

YSU_02250

CCOF:

VC76_06660(legI)

CCOO:

ATE51_00910(legI)

CAJ:	CIG1485E_0029(legI)

CIS:	CINS_1194(legI)

CVO:	CVOL_1214(legI)

CSM:	CSUB8521_1417(legI)

CSF:	CSUB8523_1517(legI)

CHV:	CHELV3228_1655(legI)

CLX:	CLAN_1241(legI)

CAVI:

CAV_1241(legI)

CAMZ:

CVIC12175_0322(legI)

CAMY:

CSUIS_1328(legI)

COJ:	CORN_1305(legI)

CUX:	CUP3940_0184(legI)

CARM:

CARM_1264(legI)

CMUC:

CMCT_0197(legI)

CNV:	CNZW441b_0374(legI)

CVU:	CVULP_0183(legI)

ABT:

ABED_0620

ATP:

ATR_1813

ACIB:

ACBT_0334

ACRE:

ACRYA_0150

ALAN:

ALANTH_0840

AELL:

AELL_2683

ACLO:

ACLO_2510

AVP:	AVENP_1995

ARC:

ABLL_0809

AROS:

NJU99_04380(neuB)

APAI:

APAC_2452

HBV:

ABIV_2490

HCJ:	HCR_18720(legI)

GLO:

Glov_3405

GSB:	GSUB_14715

PACE:

A6070_09825

PEF:	A7E78_05520 A7E78_05530

DFL:

DFE_2603

DSB:	LN040_14075

DDE:

Dde_3681

DAS:

Daes_1985

DPI:

BN4_12680

DEJ:	AWY79_10375

DDN:	DND132_0780

PSEF:

PSDVSF_07840(spsE)

PNW:	SYK_09520(legI)

PTHE:

LF599_11100

DSX:	GD604_13335

DVL:

Dvul_2590

CLIH:

KPS_002075

DOA:	AXF15_00580

DAL:

Dalk_1661

DTO:	TOL2_C35400(neuB)

DAO:	Desac_1879

BBAE:

FRD01_15935(neuB)

PSTI:

SOO65_03655

BSO:	BSNT_10307

BST:

GYO_4022

FPN:	ABE65_018195

RAX:	KO561_17085(neuB)

MEKU:

HUW50_13940(neuB) HUW50_13960

MEBZ:

LPC09_22465(neuB)

BHUI:

LOK74_08575(neuB)

PPY:

PPE_04401

PTA:	HPL003_04495

PDU:	PDUR_25200

PPEO:

ABE82_23145

PAIH:

ASL14_14800

PRZ:	GZH47_18500(neuB)

PLYC:

GXP70_28100(neuB)

PALO:

E6C60_3854

PKA:	PQ456_19785(neuB)

KEB:	GXN75_11660(neuB)

EFC:	EFAU004_01372

EFAU:

EFAU085_00958

EFU:	HMPREF0351_10916(neuB)

AUI:	APT62_03100

CARC:

NY10_960

CNO:	NT01CX_1784

CBL:

CLK_2101

CBK:

CLL_A1556

CBB:

CLD_1854

CBN:

CbC4_0996

CBT:

CLH_1466

CKL:	CKL_2109 CKL_2472

CKR:	CKR_1848 CKR_2186

CCB:	Clocel_2387

CEU:	A7L45_12145

CTAE:

BGI42_06575(neuB)

CFM:	BJL90_06975

CCHV:

BTM20_03650

CGAS:

J1C67_16000(neuB)

CMAH:

C1I91_08395(neuB)

CTAG:

LL095_12335(neuB)

CALE:

FDN13_00880(neuB)

CRS:	FQB35_02420(neuB)

CPRF:

K7H06_04875 K7H06_14850(neuB)

OCW:	OW730_17315(neuB)

CTH:

Cthe_2641

CTX:	Clo1313_0230

CCL:	Clocl_4180

SVJ:	NQ490_02935(neuB)

CMIC:

caldi_28170

DDL:	Desdi_3209

DMT:	DESME_12780

DOR:	Desor_0348

DAU:

Daud_0019

FWA:	DCMF_13725

TJR:	TherJR_2780

AWO:	Awo_c24840(neuB)

THEP:

DYI95_003445

CTHM:

CFE_2539

BPB:	bpr_I0428(neuB1)

RRK:	H8S51_002320

CSH:	Closa_2917

LXA:	OW255_01000(neuB)

LBW:	C3V36_05580(neuB)

CLE:	Clole_3572

CBAR:

PATL70BA_2643(legI)

VPY:	HZI73_23120

TEB:	T8CH_0143(legI)

CST:	CLOST_0367

TIT:

Thit_0481

TXY:	Thexy_1876

TSH:

Tsac_2668

MTA:

Moth_0756

MTHO:

MOTHE_c07000(legI)

MTHZ:

MOTHA_c07940(legI)

HOR:	Hore_16950 Hore_17000

HPK:	Hprae_0327

HALS:

D7D81_05095(neuB) D7D81_17935(neuB)

IFN:	GM661_06780(neuB) GM661_18095 GM661_18120(neuB)

AFT:	BBF96_11100

HPRF:

HLPR_26790(neuB)

CCHA:

ELD05_07925(neuB)

SPOA:

EQM13_03525(neuB)

LPIL:

LIP_2376

FCZ:	IMF26_04555(neuB)

XCL:	G4Z02_01770(neuB)

MSED:

E3O41_11625

ARI:	UM93_07605

ICA:	Intca_1261

TEH:	GKE56_00615

TED:	U5C87_11435

PLAK:

A1s21155_00145

NHI:	B1s21160_05740

PBAJ:

LRS13_09150

SYG:

sync_0174

SYW:	SYNW0448(neuB)

PMF:	P9303_01101(spsE)

PMH:	P9215_14811(spsE)

PMJ:	P9211_12451(spsE)

MPRO:

BJP34_35240

FGI:	OP10G_3269

RML:	FF011L_46220(neuB_1)

BREM:

PSR63_07865(neuB)

RLC:	K227x_18880(legI)

SNEP:

Enr13x_40700(legI)

GMR:	GmarT_17240(legI)

GIM:	F1728_06160(neuB)

GPN:	Pan110_17070(legI)

GFM:	Enr17x_19910(legI)

PHM:	PSMK_29840

PCOR:

KS4_08840(legI)

MCAD:

Pan265_05270(legI)

HBS:	IPV69_22560

UAM:	UABAM_06153

TPED:

TPE_0201

SCD:	Spica_0510

LIL:	LA_1609(spsE)

LIE:	LIF_A1296(spsE)

LIC:	LIC_12173(neuB)

LBJ:

LBJ_1133

LBL:

LBL_1187

LST:	LSS_16461 LSS_19373

LAJ:	A0128_07320 A0128_07375

LMAY:

DPV73_15970(neuB) DPV73_16020

LWL:	FHG68_08325

LKB:	LPTSP3_g21330

BPO:	BP951000_1153(spsE)

BPJ:	B2904_orf2564

BPIP:

BPP43_09345

BPW:

WESB_2577

GEX:	GETHOR_25590(spsE)

MSEA:

METESE_01910

FVA:	FV113G1_25420

FUL:	C4N20_01280(neuB)

BNO:	K8P02_14905(neuB)

BVU:

BVU_4040

BDH:	GV66_21475

TFO:	BFO_1066(neuB)

DUN:	FDZ78_02060(neuB)

DDB:	E7747_10475(neuB)

PII:	NF347_01300(neuB)

COPR:

Cop2CBH44_22070(legI)

ANF:

AQPE_0356

CHIH:

GWR21_01080(neuB) GWR21_20995(neuB)

CSAC:

SIO70_18305(neuB)

PGO:	FSB84_04825(neuB)

PEP:	AQ505_02055

PSTY:

BFS30_06850

PEJ:	FYC62_11660(neuB)

SHG:	Sph21_1815

SDJ:	NCTC13534_05285(spsE_1) NCTC13534_05286(spsE_2)

SCN:	Solca_4307

SLAS:

L2B55_03430(neuB)

MMAB:

HQ865_06375(neuB)

MGIK:

GO620_005080(neuB)

AGD:	FRZ59_07155(neuB) FRZ59_07170

OLI:	FKG96_05940(neuB)

EMAI:

KZP23_21780(neuB)

ALM:	AO498_13850

DFE:

Dfer_3363

DSN:	HWI92_21155

DYC:	NFI80_20695

DFQ:	NFI81_21770

DCN:	MUK70_26005

DPF:	ON006_22540

PACT:

CA264_12450

ASWU:

HUW51_14155(neuB)

MHAA:

Q3Y49_12135 Q3Y49_14225(neuB)

FPF:	DCC35_14845(neuB)

FBT:	D770_00730

FUV:	JR347_14630(neuB)

FMM:	LVD15_16140

FUA:	LVD17_25525

RCG:	N7E81_05775(neuB)

PRHO:

PZB74_21175

TPEL:

P0M28_29835

RSR:	T7867_07380

GFO:

GFO_0569

GRL:	LPB144_06590

GFL:

GRFL_2392

FIN:

KQS_03395

FPAL:

HYN49_06980

FSE:	DI487_03495

FEN:	J0383_14250(neuB)

FOK:	KK2020170_01100(neuB)

FAG:	K5I29_08095

COL:	AM608_07315

ZPR:

ZPR_1117

FBC:	FB2170_05795

MART:

BTR34_08470

MARB:

CJ263_08140(neuB)

LVN:	BWR22_10530

PSYD:

IMZ30_08095

NDO:

DDD_3554

NOB:	CW736_09215

NOJ:	EJ995_10795

NMF:

NMS_2733

POM:	MED152_05685

POLA:

BXQ17_13925

PHAL:

H9I45_03450

PPEC:

H9W90_01215

PBAT:

JL193_16055

PCEA:

J3359_01875

WIJ:	BWZ20_06350

TJE:	TJEJU_1144(legI)

LUL:	LPB138_01915

WFU:	AXE80_08450

FOP:	FNB79_09370

SALT:

AO058_07925

SMIS:

LDL76_16105

SEON:

BWZ22_01500

KOS:	KORDIASMS9_03609(legI)

KAN:	IMCC3317_05800(legI)

AFLA:

FHG64_14730

ANP:	FK178_08465

ALTI:

ALE3EI_0110

MUT:	GVT53_17455(neuB)

SAHN:

JRG66_01115(neuB)

AEST:

RBH94_01335

CJG:	NCTC13459_02715(spsE)

CANT:

NCTC13489_01541(spsE)

FTE:	Fluta_2184

CHYD:

H4K34_10465

PPH:

Ppha_0527

PMX:	PERMA_1627(neuB)

TTK:	TST_1473(neuB)

TNP:

Tnap_0417

MAEO:

JRV97_05655(neuB)

DDF:	DEFDS_0370

DAP:	Dacet_0450

DTP:	JZK55_10000

NLI:	G3M70_03950 G3M70_11130(neuB)

NWT:	NSPWAT_0140(neuB)

NVA:	G3M78_00515

NBN:	HYY55_00040(neuB)

MMQ:	MmarC5_0506

MMAK:

MMKA1_03760

MAE:

Maeo_0420

METJ:

FZP68_08655(neuB)

MEW:	MSWAN_1357 MSWAN_1360

MSJ:	MSSAC_2015

MSW:	MSSIT_2302

MBU:

Mbur_1586

MRTJ:

KHC33_01430

MEND:

L6E24_13055(neuB)

MESB:

L1S32_02480(neuB)

MFO:	Metfor_1307

SAIM:

K0C01_02745(neuB)

HMU:

Hmuk_1456

HALZ:

E5139_10440(neuB)

HAZP:

GBQ70_10435(neuB)

HPEL:

HZS54_05935

HALP:

DOS48_05315(neuB)

HAE:	halTADL_2399

NMG:	Nmag_0151(neuB)

NID:	NPIRD3C_0036

NIN:	NADRNF5_2117

NIW:	Nisw_04230

NUE:	C5F50_00695

NCT:

NMSP_0147

NIC:	DSQ20_00905

NDV:	NDEV_0150(NeuB) NDEV_0210(neuB2) NDEV_0212

» show all

Reference

1 [PMID:18275154]

Authors

Glaze PA, Watson DC, Young NM, Tanner ME

Title

Biosynthesis of CMP-N,N'-diacetyllegionaminic acid from UDP-N,N'-diacetylbacillosamine in Legionella pneumophila.

Journal

Biochemistry 47:3272-82 (2008)
DOI:10.1021/bi702364s

Sequence

[lpn:lpg0752]

Reference

2 [PMID:19282391]

Authors

Schoenhofen IC, Vinogradov E, Whitfield DM, Brisson JR, Logan SM

Title

The CMP-legionaminic acid pathway in Campylobacter: biosynthesis involving novel GDP-linked precursors.

Journal

Glycobiology 19:715-25 (2009)
DOI:10.1093/glycob/cwp039

Sequence

[cje:Cj1327]

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.101

IUBMB Enzyme Nomenclature:

2.5.1.101

ExPASy - ENZYME nomenclature database:

2.5.1.101

BRENDA, the Enzyme Database:

2.5.1.101

DBGET integrated database retrieval system