KEGG   ENZYME: 2.5.1.114Help
Entry
EC 2.5.1.114                Enzyme                                 

Name
tRNAPhe (4-demethylwyosine37-C7) aminocarboxypropyltransferase;
TYW2;
tRNA-yW synthesizing enzyme-2;
TRM12 (gene name);
taw2 (gene name)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
S-adenosyl-L-methionine:tRNAPhe (4-demethylwyosine37-C7)-[(3S)-3-amino-3-carboxypropyl]transferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 4-demethylwyosine37 in tRNAPhe = S-methyl-5'-thioadenosine + 7-[(3S)-3-amino-3-carboxypropyl]-4-demethylwyosine37 in tRNAPhe [RN:R10611]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
4-demethylwyosine37 in tRNAPhe
Product
S-methyl-5'-thioadenosine [CPD:C00170];
7-[(3S)-3-amino-3-carboxypropyl]-4-demethylwyosine37 in tRNAPhe
Comment
The enzyme, which is found in all eukaryotes and in the majority of Euryarchaeota (but not in the Crenarchaeota), is involved in the hypermodification of the guanine nucleoside at position 37 of tRNA leading to formation of assorted wye bases. This modification is essential for translational reading-frame maintenance. The eukaryotic enzyme is involved in biosynthesis of the tricyclic base wybutosine, which is found only in tRNAPhe.
History
EC 2.5.1.114 created 2013
Orthology
K07055  tRNA wybutosine-synthesizing protein 2
Genes
HSA: 55039(TRMT12)
PTR: 464377(TRMT12)
PPS: 100979722(TRMT12)
GGO: 101124627(TRMT12)
PON: 100447332(TRMT12)
NLE: 100582652(TRMT12)
MCC: 705848(TRMT12)
CSAB: 103237404(TRMT12)
RRO: 104662794(TRMT12)
RBB: 108538090(TRMT12)
CJC: 100385176(TRMT12)
SBQ: 101029907(TRMT12)
MMU: 68260(Trmt12)
RNO: 314999(Trmt12)
CGE: 100772520(Trmt12)
NGI: 103743903(Trmt12)
HGL: 101715645(Trmt12)
CCAN: 109688115(Trmt12)
OCU: 100352397(TRMT12)
TUP: 102500902(TRMT12)
CFA: 609411(TRMT12)
AML: 100468536(TRMT12)
UMR: 103661618(TRMT12)
ORO: 101384928(TRMT12)
FCA: 101085181(TRMT12)
PTG: 102970870(TRMT12)
AJU: 106986564(TRMT12)
BTA: 516516(TRMT12)
BOM: 102277834(TRMT12)
BIU: 109568571(TRMT12)
PHD: 102315090(TRMT12)
CHX: 102189221(TRMT12)
OAS: 101106695(TRMT12)
SSC: 100155406(TRMT12)
CFR: 102519623(TRMT12)
CDK: 105096435(TRMT12)
BACU: 103011113(TRMT12)
LVE: 103086079(TRMT12)
OOR: 101277734(TRMT12)
ECB: 100067346(TRMT12)
EPZ: 103564519(TRMT12)
EAI: 106839357(TRMT12)
MYB: 106723536(TRMT12)
MYD: 102770501(TRMT12)
RSS: 109440594(TRMT12)
PALE: 102896712(TRMT12)
LAV: 100658475(TRMT12)
TMU: 101347238
MDO: 100020165(TRMT12) 100023277
SHR: 100926005(TRMT12)
OAA: 100087951(TRMT12)
GGA: 422259(TRMT12)
MGP: 100538997(TRMT12)
CJO: 107312716(TRMT12)
ACYG: 106037483(TRMT12)
TGU: 100226909(TRMT12)
FAB: 101812632(TRMT12)
PHI: 102114676(TRMT12)
PMAJ: 107204035(TRMT12)
CCAE: 111928720(TRMT12)
CCW: 104691361(TRMT12)
FPG: 101916683(TRMT12)
FCH: 102054438(TRMT12)
CLV: 102096517
EGZ: 104129953(TRMT12)
AAM: 106484769(TRMT12)
ASN: 102382316(TRMT12)
AMJ: 102563349(TRMT12)
PSS: 102456684(TRMT12)
CMY: 102935765
CPIC: 101946914(TRMT12)
ACS: 107982969(trmt12)
PVT: 110083587(TRMT12)
PBI: 103062147(TRMT12)
GJA: 107124532(TRMT12)
XLA: 108698285
XTR: 100380191(trmt12)
NPR: 108799572(TRMT12)
DRE: 564925(trmt12)
SRX: 107740100(trmt12)
SANH: 107669089(trmt12)
SGH: 107602575(trmt12)
IPU: 108268801(trmt12)
AMEX: 103042921(trmt12)
TRU: 101061055(trmt12)
LCO: 104928726(trmt12)
NCC: 104955942(trmt12)
MZE: 101477717(trmt12)
OLA: 101163092(trmt12)
XMA: 102227936(trmt12)
PRET: 103471570(trmt12)
NFU: 107378195(trmt12)
KMR: 108231272(trmt12)
CSEM: 103390537(trmt12)
LCF: 108872492(trmt12)
SDU: 111232645(trmt12)
HCQ: 109524300(trmt12)
BPEC: 110159037(trmt12)
MALB: 109955755(trmt12)
SASA: 106611794
OTW: 112255681(trmt12)
ELS: 105029873(trmt12)
SFM: 108923167(trmt12)
LCM: 102350688(TRMT12)
CMK: 103177199(trmt12)
CIN: 100176207
SPU: 593691
APLC: 110982434
SKO: 100379063
CLEC: 106669520
ZNE: 110836641
FCD: 110859278
CEL: CELE_F40F9.10(F40F9.10)
CBR: CBG19352
BMY: Bm1_51625
TSP: Tsp_06313
CRG: 105332610
MYI: 110445483
OBI: 106883232
EPA: 110238807
ADF: 107333786
HMG: 100209695
OEU: 111376906
SCE: YML005W(TRM12)
NCS: NCAS_0G03740(NCAS0G03740)
NDI: NDAI_0G00730(NDAI0G00730)
TPF: TPHA_0G00810(TPHA0G00810)
TBL: TBLA_0B06040(TBLA0B06040)
TDL: TDEL_0G02320(TDEL0G02320)
KAF: KAFR_0H03230(KAFR0H03230)
CAL: CAALFM_C305890WA(TRM12)
CAUR: QG37_00338
SLB: AWJ20_4217(TRM12)
NCR: NCU10483
NTE: NEUTE1DRAFT14617(NEUTE1DRAFT_14617)
MGR: MGG_12102
SSCK: SPSK_00267
MAW: MAC_00071
MAJ: MAA_01780
CMT: CCM_05153
MBE: MBM_06394
ANI: AN0130.2
ANG: ANI_1_1248164(An18g03090)
ABE: ARB_03105
TVE: TRV_05817
PTE: PTT_04003
SPO: SPAC4G8.06c(trm12)
PYO: PY17X_0503900(PY05008)
MJA: MJ_1557
MMP: MMP0560
MMD: GYY_03680
MAE: Maeo_0636
MVO: Mvol_0432
MTH: MTH_1200
METC: MTCT_1093
MWO: MWSIV6_1593(taw2)
METE: tca_01157
MST: Msp_0297
MSI: Msm_1036
MRU: mru_1976
MEB: Abm4_1548
MMIL: sm9_2048
MEYE: TL18_09425
MOL: YLM1_1413
METH: MBMB1_1722(taw2)
MFC: BRM9_0347
MFI: DSM1535_0950(taw2)
MCUB: MCBB_2023(taw2)
MFV: Mfer_0394
MKA: MK0940
AFU: AF_1973
FPL: Ferp_1396
GAC: GACE_0368
GAH: GAH_01121
TAC: Ta0926
TVO: TVG1132799(TVG1132799)
PTO: PTO0680
FAI: FAD_0193
CDIV: CPM_1505
MEAR: Mpt1_c12780(taw2)
MARC: AR505_1645
ABI: Aboo_0011
PHO: PH0793(PH0793)
PAB: PAB1538
PFU: PF0500
PFI: PFC_01620
PYN: PNA2_1434
PYS: Py04_0683
TKO: TK0071
TON: TON_0748
TGA: TGAM_0679
TSI: TSIB_1665
THE: GQS_01185
THA: TAM4_598
THM: CL1_1935
TLT: OCC_03207
THS: TES1_0720
TNU: BD01_0979
TEU: TEU_10690
PPAC: PAP_02950
MAC: MA_2064
MBAR: MSBR2_0721
MBAK: MSBR3_0463
MMA: MM_3150
MMAC: MSMAC_2878
METM: MSMTP_2061
MTHE: MSTHC_0281
MTHR: MSTHT_0440
MHOR: MSHOH_1874
MBU: Mbur_0655
MMET: MCMEM_0806
MMH: Mmah_1140
MPY: Mpsy_3091
MTP: Mthe_0736
MCJ: MCON_1956
MHI: Mhar_0127
MHU: Mhun_0829
MLA: Mlab_0923
MEMA: MMAB1_1318
MPI: Mpet_0984
MBN: Mboo_1668
MPL: Mpal_1885
MPD: MCP_0205
MEZ: Mtc_2504
RCI: RCIX1355
HAL: VNG_2475C
HSL: OE_4468F(taw2)
HHB: Hhub_3706(taw2)
HALH: HTSR_2016
HHSR: HSR6_2092
HMA: rrnAC2902
HHI: HAH_0171
NPH: NP_1360A(taw2)
NMO: Nmlp_1176(taw2)
HUT: Huta_1011
HTI: HTIA_0874
HMU: Hmuk_1200
HTU: Htur_0202
NMG: Nmag_2032(taw2)
NAT: NJ7G_0351
SALI: L593_09850
STO: STK_21320
SSO: SSO2223
SOL: Ssol_0041
SSOA: SULA_0039
SSOL: SULB_0040
SSOF: SULC_0039
SAI: Saci_0030
SID: M164_0039
SII: LD85_0039
SIH: SiH_0039
SIR: SiRe_0039
SIC: SiL_0039
MSE: Msed_0744
MCN: Mcup_1354
AHO: Ahos_2318
LOKI: Lokiarch_25700(taw2_1) Lokiarch_30240(taw2_2)
 » show all
Taxonomy
Reference
1  [PMID:19717466]
  Authors
Umitsu M, Nishimasu H, Noma A, Suzuki T, Ishitani R, Nureki O
  Title
Structural basis of AdoMet-dependent aminocarboxypropyl transfer reaction catalyzed by tRNA-wybutosine synthesizing enzyme, TYW2.
  Journal
Proc Natl Acad Sci U S A 106:15616-21 (2009)
DOI:10.1073/pnas.0905270106
  Sequence
[mja:MJ_1557]
Reference
2  [PMID:22761755]
  Authors
Rodriguez V, Vasudevan S, Noma A, Carlson BA, Green JE, Suzuki T, Chandrasekharappa SC
  Title
Structure-function analysis of human TYW2 enzyme required for the biosynthesis of a highly modified Wybutosine (yW) base in phenylalanine-tRNA.
  Journal
PLoS One 7:e39297 (2012)
DOI:10.1371/journal.pone.0039297
Reference
3  [PMID:20382657]
  Authors
de Crecy-Lagard V, Brochier-Armanet C, Urbonavicius J, Fernandez B, Phillips G, Lyons B, Noma A, Alvarez S, Droogmans L, Armengaud J, Grosjean H
  Title
Biosynthesis of wyosine derivatives in tRNA: an ancient and highly diverse pathway in Archaea.
  Journal
Mol Biol Evol 27:2062-77 (2010)
DOI:10.1093/molbev/msq096
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.114
IUBMB Enzyme Nomenclature: 2.5.1.114
ExPASy - ENZYME nomenclature database: 2.5.1.114
BRENDA, the Enzyme Database: 2.5.1.114

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