KEGG   ENZYME: 2.5.1.46Help
Entry
EC 2.5.1.46                 Enzyme                                 

Name
deoxyhypusine synthase;
spermidine:eIF5A-lysine 4-aminobutyltransferase (propane-1,3-diamine-forming)
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
[eIF5A-precursor]-lysine:spermidine 4-aminobutyltransferase (propane-1,3-diamine-forming)
Reaction(IUBMB)
[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine (overall reaction) [RN:R05329];
(1a) spermidine + NAD+ = dehydrospermidine + NADH [RN:R07477];
(1b) dehydrospermidine + [enzyme]-lysine = N-(4-aminobutylidene)-[enzyme]-lysine + propane-1,3-diamine [RN:R07479];
(1c) N-(4-aminobutylidene)-[enzyme]-lysine + [eIF5A-precursor]-lysine = N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + [enzyme]-lysine [RN:R07480];
(1d) N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + NADH + H+ = [eIF5A-precursor]-deoxyhypusine + NAD+ [RN:R07478]
Reaction(KEGG)
Substrate
[eIF5A-precursor]-lysine [CPD:C07281];
spermidine [CPD:C00315];
NAD+ [CPD:C00003];
dehydrospermidine [CPD:C15853];
[enzyme]-lysine [CPD:C07281];
N-(4-aminobutylidene)-[enzyme]-lysine [CPD:C15854];
N-(4-aminobutylidene)-[eIF5A-precursor]-lysine [CPD:C15854];
NADH [CPD:C00004];
H+ [CPD:C00080]
Product
[eIF5A-precursor]-deoxyhypusine [CPD:C07282];
propane-1,3-diamine [CPD:C00986];
dehydrospermidine [CPD:C15853];
NADH [CPD:C00004];
N-(4-aminobutylidene)-[enzyme]-lysine [CPD:C15854];
N-(4-aminobutylidene)-[eIF5A-precursor]-lysine [CPD:C15854];
[enzyme]-lysine [CPD:C07281];
NAD+ [CPD:C00003]
Comment
The eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity. This enzyme catalyses the first reaction of hypusine formation from one specific lysine residue of the eIF5A precursor. The reaction occurs in four steps: NAD+-dependent dehydrogenation of spermidine (1a), formation of an enzyme-imine intermediate by transfer of the 4-aminobutylidene group from dehydrospermidine to the active site lysine residue (Lys329 for the human enzyme; 1b), transfer of the same 4-aminobutylidene group from the enzyme intermediate to the e1F5A precursor (1c), reduction of the e1F5A-imine intermediate to form a deoxyhypusine residue (1d). Hence the overall reaction is transfer of a 4-aminobutyl group. For the plant enzyme, homospermidine can substitute for spermidine and putrescine can substitute for the lysine residue of the eIF5A precursor. Hypusine is formed from deoxyhypusine by the action of EC 1.14.99.29, deoxyhypusine monooxygenase.
History
EC 2.5.1.46 provisional version created 1999 as EC 1.1.1.249 deleted 1999, revised and reinstated 2001 as EC 2.5.1.46
Orthology
K00809  deoxyhypusine synthase
Genes
HSA: 1725(DHPS)
PTR: 455746(DHPS)
PPS: 100977998(DHPS)
GGO: 101136731(DHPS)
PON: 100443233(DHPS)
NLE: 100597140(DHPS)
MCC: 716213(DHPS)
MCF: 102138960(DHPS)
CSAB: 103233979(DHPS)
RRO: 104661101(DHPS)
RBB: 108539610(DHPS)
CJC: 100396022(DHPS)
SBQ: 101050408(DHPS)
MMU: 330817(Dhps)
RNO: 288923(Dhps)
CGE: 100755665(Dhps)
NGI: 103751894(Dhps)
HGL: 101708097(Dhps)
CCAN: 109681277(Dhps)
OCU: 100352059(DHPS)
TUP: 102485533(DHPS)
CFA: 476702(DHPS)
AML: 100474462(DHPS)
UMR: 103682157(DHPS)
ORO: 101368037(DHPS)
FCA: 101083659(DHPS)
PTG: 102959277(DHPS)
AJU: 106985534(DHPS)
BTA: 444988(DHPS)
BOM: 102270034(DHPS)
BIU: 109561548(DHPS)
PHD: 102336679(DHPS)
CHX: 102171505(DHPS)
OAS: 101107277(DHPS)
SSC: 100519413(DHPS)
CFR: 102505713(DHPS)
CDK: 105104065(DHPS)
BACU: 103000331(DHPS)
LVE: 103087338(DHPS)
OOR: 101284267(DHPS)
ECB: 100063550(DHPS)
EPZ: 103542773(DHPS)
EAI: 106826924(DHPS)
MYB: 102255457(DHPS)
MYD: 102755857(DHPS)
HAI: 109394256(DHPS)
RSS: 109454957(DHPS)
PALE: 102898526(DHPS)
LAV: 100656596(DHPS)
TMU: 101343153
MDO: 100010290(DHPS)
SHR: 100920525(DHPS)
OAA: 103170595(DHPS)
GGA: 107057627(DHPS)
CJO: 107307164(DHPS)
PHI: 102101132(DHPS)
FPG: 106112398(DHPS)
FCH: 106630863(DHPS)
AAM: 106486625(DHPS)
ASN: 102377261(DHPS)
AMJ: 102562780(DHPS)
PSS: 102453700(DHPS)
CMY: 102935067(DHPS)
CPIC: 101953196(DHPS)
ACS: 100553765(dhps)
PVT: 110077395(DHPS)
PBI: 103053909(DHPS)
GJA: 107120598(DHPS)
XLA: 108711712(dhps.L) 431985(dhps.S)
XTR: 394501(dhps)
NPR: 108794974(DHPS) 108800745
DRE: 406329(dhps)
SRX: 107718601 107753765(dhps)
CCAR: 109047440
IPU: 100528141(dhps)
AMEX: 103031482(dhps)
TRU: 101063502(dhps) 101074180
LCO: 104934332(dhps)
OLA: 101171006(dhps)
XMA: 102227615(dhps)
PRET: 103469305(dhps)
NFU: 107376607(dhps)
CSEM: 103392943(dhps) 103392944
HCQ: 109527046(dhps)
ELS: 105012887(dhps) 105012888
SFM: 108926075(dhps)
LCM: 102358518(DHPS)
CMK: 103187570(dhps)
CIN: 100187438
SPU: 579530(dhps) 582547
APLC: 110981409
SKO: 100373608
DME: Dmel_CG8005(CG8005)
DSI: Dsimw501_GD14061(Dsim_GD14061)
MDE: 101900686
AAG: 5574567
AME: 100578266
BIM: 100745513
SOC: 105201636
AEC: 105144800
ACEP: 105624652
PBAR: 105422536
HST: 105186729
CFO: 105259058
LHU: 105678597
PGC: 109862400
NVI: 100114756
TCA: 659163
DPA: 109546818
NVL: 108557196
BMOR: 100190959(Dhs)
PMAC: 106708105
PRAP: 111002931
PXY: 105385059
API: 100160249
DNX: 107172072
ZNE: 110839256
FCD: 110851734
TUT: 107368689
CEL: CELE_Y17G7B.4(dhps-1)
CBR: CBG21024
BMY: Bm1_16300
TSP: Tsp_06824
CRG: 105339234
MYI: 110453567
OBI: 106874794
LAK: 106160869
SHX: MS3_01302
EGL: EGR_00307
EPA: 110250271
ADF: 107332977
AQU: 100640245
ATH: AT5G05920(DHS)
CRB: 17881605
CPAP: 110819197
CIT: 102616285
TCC: 18585734
DZI: 111310078
EGR: 104436515
GMX: 100305453
VRA: 106764595
VAR: 108345960
CCAJ: 109818884
LJA: Lj1g3v3646510.1(Lj1g3v3646510.1) Lj1g3v3646510.2(Lj1g3v3646510.2)
ADU: 107490155
AIP: 107644469
LANG: 109351093
FVE: 101308495
PPER: 18769561
PAVI: 110750964
PXB: 103943952
ZJU: 107424009
CSV: 101220126
RCU: 8271139
JCU: 105650962
HBR: 110671250
POP: 7462832
VVI: 100247561
SLY: 101249422 543666(DHS)
CANN: 107859602
NTA: 107783753 107796463(DHS1)
NSY: 104231513
NTO: 104090458
SIND: 105173586
DCR: 108213029
BVG: 104896269
NNU: 104604419
OSA: 4334056
DOSA: Os03t0740600-01(Os03g0740600) Os09t0421100-00(Os09g0421100)
BDI: 100823824
ATS: 109756786(LOC109756786) 109782584(LOC109782584) 109782592(LOC109782592)
ZMA: 100191910(dhs2) 100282705(dhs1)
SITA: 101756334
PDA: 103721451
EGU: 105039714
MUS: 103987529
ATR: 18443737
APRO: F751_4451
SCE: YHR068W(DYS1)
ERC: Ecym_3222
KMX: KLMA_70371(DYS1)
NCS: NCAS_0H01680(NCAS0H01680)
NDI: NDAI_0C02010(NDAI0C02010)
TPF: TPHA_0D02020(TPHA0D02020)
TBL: TBLA_0C03540(TBLA0C03540)
TDL: TDEL_0E02840(TDEL0E02840)
KAF: KAFR_0K02250(KAFR0K02250)
CAL: CAALFM_C302180CA(CaO19.1626)
CAUR: QG37_07194
SLB: AWJ20_4378(DYS1)
NCR: NCU05418(dys-1)
NTE: NEUTE1DRAFT65655(NEUTE1DRAFT_65655)
MGR: MGG_09173
SSCK: SPSK_00293
TRE: TRIREDRAFT_54448(dhyS)
MAW: MAC_06555
MAJ: MAA_07630
CMT: CCM_04765
BFU: BCIN_07g02080(Bcdys1)
MBE: MBM_05481
ANI: AN8075.2
ANG: ANI_1_190144(An16g01480)
ABE: ARB_00858
TVE: TRV_07899
PTE: PTT_18821
CNE: CNJ02480
CNB: CNBJ0980
ABP: AGABI1DRAFT113512(AGABI1DRAFT_113512)
ABV: AGABI2DRAFT191839(AGABI2DRAFT_191839)
MGL: MGL_0173
DDI: DDB_G0285725(dhps)
DFA: DFA_09448(dhps)
EHI: EHI_006030(83.t00016) EHI_098350(77.t00015)
PYO: PY17X_1032400(PY01546)
PCB: PCHAS_103080(PC301479.00.0)
TAN: TA13570
TPV: TP02_0058
BBO: BBOV_III010890(17.m07937)
CPV: cgd2_3930
SMIN: v1.2.000432.t1(symbB.v1.2.000432.t1)
TPS: THAPSDRAFT_268986(dhs1)
SPAR: SPRG_01451
LIF: LINJ_20_0270(DHS20) LINJ_34_0350(DHS34)
CBU: CBU_0721
CBD: CBUD_0732
CBG: CbuG_1281
CBC: CbuK_1537
DMA: DMR_24290
DGG: DGI_1717
DRT: Dret_1312
ADE: Adeh_3097
SCL: sce5982 sce7004(dys)
SAT: SYN_02461
SFU: Sfum_3135
BBA: Bd2349(dys)
BBAT: Bdt_2302(dys)
BBW: BDW_08730
BBAC: EP01_07995
BEX: A11Q_1017
AOL: S58_72380
CCR: CC_0359
CAK: Caul_0257
CSE: Cseg_3779
PZU: PHZ_c3320
ZMO: ZMO1019
ZMN: Za10_0286
ZMM: Zmob_0291
ZMB: ZZ6_0291
ZMI: ZCP4_0299
SWI: Swit_4663
SPHD: HY78_01820
SPHM: G432_03450
STAX: MC45_03740
SPHI: TS85_12070
SSAN: NX02_16895
SPKC: KC8_01335
PUB: SAR11_0205(dys2)
CYA: CYA_2657
CYB: CYB_2420
TEL: tlr0818
GVI: glr1045
GLJ: GKIL_1895
ANA: alr3804
NAZ: Aazo_1485
CALH: IJ00_03960
CTHE: Chro_1131
RCA: Rcas_3399
CAU: Caur_0545
CAG: Cagg_3460
HAU: Haur_3346
TRA: Trad_0088
TTH: TT_C1205
TTJ: TTHA1570
TAQ: TO73_0420
MRB: Mrub_2079
PNL: PNK_1142(dys)
PUV: PUV_04440
OTE: Oter_1628
MIN: Minf_2205(dys1)
RBA: RB1381(dys1)
PSL: Psta_0402
TTF: THTE_3185
IPA: Isop_1096
KST: KSMBR1_3542(dys-1)
ACA: ACP_1729
ABAS: ACPOL_0481
SUS: Acid_1177
EMI: Emin_1464
GAU: GAU_2131
GBA: J421_3735
MBAS: ALGA_4366
CPI: Cpin_0490
FLN: FLA_5889
PHE: Phep_0287
MUC: MuYL_0599
CHU: CHU_1939
HSW: Hsw_2920
FLM: MY04_4887
GFO: GFO_3623
GFL: GRFL_1787
FJO: Fjoh_2810
FPS: FP1709
FIN: KQS_06770
COC: Coch_1873
ZPR: ZPR_4204
RAI: RA0C_1041
RAR: RIA_1448
RAG: B739_1077
RAE: G148_0821
RAT: M949_1544
MARM: YQ22_09765
CBAL: M667_06550
CBAT: M666_06570
DOK: MED134_05899(dhys)
DDO: I597_2169
MLT: VC82_1630
NDO: DDD_1957
EAO: BD94_0576
CHZ: CHSO_4280
TMAR: MARIT_0341
MARF: CJ739_902
FBA: FIC_00242
BPI: BPLAN_485
BMM: MADAR_463
BCP: BLBCPU_138(dys1)
BBG: BGIGA_478(dys1)
BLP: BPAA_486
BLU: K645_731
CTE: CT0011
CPC: Cpar_2043
CCH: Cag_0073
CLI: Clim_2480
PVI: Cvib_1729
PLT: Plut_2089
PPH: Ppha_2876
PAA: Paes_2235
CTS: Ctha_1417
IAL: IALB_0664(dys)
MRO: MROS_1683
NDE: NIDE3977
MJA: MJ_0814
MMP: MMP0137(dys)
MMD: GYY_00695
MAE: Maeo_1473
MVO: Mvol_1228
MTH: MTH_127
MMG: MTBMA_c05770(dys)
METC: MTCT_0092
MWO: MWSIV6_0542(dys)
METE: tca_00111
MST: Msp_0395(dys)
MSI: Msm_1615
MRU: mru_1053(dys)
MEB: Abm4_0746(dhys)
MMIL: sm9_1413(dys)
MEYE: TL18_03975
MOL: YLM1_0596
METH: MBMB1_0656(dys)
MFC: BRM9_1536(dys)
MFI: DSM1535_2240(dys)
MCUB: MCBB_0671(dys)
MFV: Mfer_0088
MKA: MK0737(dys1)
GAC: GACE_0691
GAH: GAH_02017
TAC: Ta0356
TVO: TVG0406345(TVG0406345)
PTO: PTO1252
FAI: FAD_0214
CDIV: CPM_0264
MARC: AR505_1375
ABI: Aboo_0041
PHO: PH1397(PH1397)
PAB: PAB0511(dys1)
PFU: PF0292
PFI: PFC_00525
PYN: PNA2_0805
PYS: Py04_0650
TKO: TK0671
TON: TON_0701
TGA: TGAM_1020(dhys)
TSI: TSIB_0958
THE: GQS_01375
THA: TAM4_660
THM: CL1_1999
TLT: OCC_07294
THS: TES1_1326
TNU: BD01_0653
TEU: TEU_07815
PPAC: PAP_08250
MAC: MA_0968 MA_3013(dys)
MBU: Mbur_1868
MMET: MCMEM_0098
MMH: Mmah_1888
MCJ: MCON_3059(dysA) MCON_3163(dysB)
MLA: Mlab_0154
MEMA: MMAB1_0754(dys) MMAB1_3348(dys)
MPI: Mpet_0152
MBN: Mboo_0184
MPD: MCP_0098(dys-1) MCP_1441(dys-2)
MEZ: Mtc_0598(dys-1) Mtc_1365(dys-2)
RCI: RCIX304(dys-1) RRC163(dys-2)
HAL: VNG_1432G(dhs)
HSL: OE_3059F(dhs)
HHB: Hhub_2865(dhs)
HALH: HTSR_0419
HHSR: HSR6_0404(dhs)
HMA: rrnAC1918(dhs)
HHI: HAH_2429(dhs)
NPH: NP_4578A(dhs)
NMO: Nmlp_3450(dhs)
HUT: Huta_2574
HMU: Hmuk_1329
HWA: HQ_2852A(dhs)
HWC: Hqrw_3242(dhs)
HVO: HVO_2297(dhs1) HVO_B0182(dhs2)
HME: HFX_2305(dhs) HFX_5233(dhs)
HLA: Hlac_1997
HTU: Htur_3109
NMG: Nmag_3738(dhs)
NAT: NJ7G_2657
SALI: L593_03400
APE: APE_0698.1(dys)
ACJ: ACAM_0515(dys)
IHO: Igni_0631
IIS: EYM_05545
HBU: Hbut_0040
STO: STK_12930(dys)
SSO: SSO0967
SOL: Ssol_1946
SSOA: SULA_1978
SSOL: SULB_1979
SSOF: SULC_1977
SAI: Saci_1316
SID: M164_1240
SII: LD85_1368
SIH: SiH_1201
SIR: SiRe_1119
SIC: SiL_1116
MSE: Msed_1753
MCN: Mcup_0476
AHO: Ahos_1418
PAI: PAE3487
PIS: Pisl_0783
PCL: Pcal_1171
PAS: Pars_1970
PYR: P186_0643
POG: Pogu_0152
TNE: Tneu_0861
CMA: Cmaq_0976
TUZ: TUZN_0371
TTN: TTX_0721(dys)
VDI: Vdis_2347
VMO: VMUT_0699
TPE: Tpen_0413
ASC: ASAC_0219
ACIA: SE86_01890
NGA: Ngar_c06230(dys1) Ngar_c06260(dys2)
NVN: NVIE_025230(dys1) NVIE_025240(dys2)
NCV: NCAV_1189(dys) NCAV_1306(HGP)
NDV: NDEV_1141 NDEV_1183(dys)
NEQ: NEQ402
NAA: Nps_02765
MARH: Mia14_0922
KCR: Kcr_0505
 » show all
Taxonomy
Reference
1  [PMID:2108161]
  Authors
Wolff EC, Park MH, Folk JE
  Title
Cleavage of spermidine as the first step in deoxyhypusine synthesis. The role of  NAD.
  Journal
J Biol Chem 265:4793-9 (1990)
Reference
2  [PMID:9188485]
  Authors
Wolff EC, Folk JE, Park MH.
  Title
Enzyme-substrate intermediate formation at lysine 329 of human deoxyhypusine synthase.
  Journal
J Biol Chem 272:15865-71 (1997)
DOI:10.1074/jbc.272.25.15865
Reference
3  [PMID:9285092]
  Authors
Chen KY, Liu AY.
  Title
Biochemistry and function of hypusine formation on eukaryotic initiation factor 5A.
  Journal
Biol Signals 6:105-9 (1997)
Reference
4  [PMID:10542236]
  Authors
Ober D, Hartmann T.
  Title
Deoxyhypusine synthase from tobacco. cDNA isolation, characterization, and bacterial expression of an enzyme with extended substrate specificity.
  Journal
J Biol Chem 274:32040-7 (1999)
DOI:10.1074/jbc.274.45.32040
  Sequence
Reference
5  [PMID:10611289]
  Authors
Ober D, Hartmann T.
  Title
Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase.
  Journal
Proc Natl Acad Sci U S A 96:14777-82 (1999)
DOI:10.1073/pnas.96.26.14777
  Sequence
Reference
6  [PMID:10028184]
  Authors
Wolff EC, Park MH.
  Title
Identification of lysine350 of yeast deoxyhypusine synthase as the site of enzyme intermediate formation.
  Journal
Reference
7  [PMID:10734052]
  Authors
Wolff EC, Wolff J, Park MH.
  Title
Deoxyhypusine synthase generates and uses bound NADH in a transient hydride transfer mechanism.
  Journal
J Biol Chem 275:9170-7 (2000)
DOI:10.1074/jbc.275.13.9170
Reference
8  [PMID:7673224]
  Authors
Joe YA, Wolff EC, Park MH.
  Title
Cloning and expression of human deoxyhypusine synthase cDNA. Structure-function studies with the recombinant enzyme and mutant proteins.
  Journal
J Biol Chem 270:22386-92 (1995)
DOI:10.1074/jbc.270.38.22386
  Sequence
[hsa:1725]
Reference
9  [PMID:7592594]
  Authors
Tao Y, Chen KY.
  Title
Molecular cloning and functional expression of Neurospora deoxyhypusine synthase cDNA and identification of yeast deoxyhypusine synthase cDNA.
  Journal
J Biol Chem 270:23984-7 (1995)
DOI:10.1074/jbc.270.41.23984
  Sequence
[ncr:NCU05418]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.46
IUBMB Enzyme Nomenclature: 2.5.1.46
ExPASy - ENZYME nomenclature database: 2.5.1.46
BRENDA, the Enzyme Database: 2.5.1.46
CAS: 127069-31-2

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