KEGG   ENZYME: 2.5.1.73Help
Entry
EC 2.5.1.73                 Enzyme                                 

Name
O-phospho-L-seryl-tRNA:Cys-tRNA synthase;
SepCysS;
Sep-tRNA:Cys-tRNA synthase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
O-phospho-L-seryl-tRNACys:hydrogen sulfide 2-aminopropanoate transferase
Reaction(IUBMB)
O-phospho-L-seryl-tRNACys + sulfide = L-cysteinyl-tRNACys + phosphate [RN:R08577]
Reaction(KEGG)
Substrate
O-phospho-L-seryl-tRNACys;
sulfide [CPD:C00283]
Product
L-cysteinyl-tRNA(Cys) [CPD:C03125];
phosphate [CPD:C00009]
Comment
In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [1]. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) and EC 2.5.1.73 [2].
History
EC 2.5.1.73 created 2009
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K06868  Sep-tRNA:Cys-tRNA synthetase
Genes
MJA: MJ_1678
MFE: Mefer_0975
MVU: Metvu_1276
MFS: MFS40622_0574
MIF: Metin_0086
MJH: JH146_1495
MIG: Metig_1345
MMP: MMP1240
MMQ: MmarC5_0351
MMX: MmarC6_1433
MMD: GYY_07035
MAE: Maeo_0681
MVO: Mvol_0651
MTH: MTH_1067
MMG: MTBMA_c14550(pscS)
METC: MTCT_0968
METE: tca_01031(iscS)
METH: MBMB1_0325
MFC: BRM9_1944(pscS)
MCUB: MCBB_0296
MFV: Mfer_0642
MKA: MK0229
FPL: Ferp_2023
GAC: GACE_1091
MAC: MA_0722
MBAR: MSBR2_2871
MMA: MM_1881
MMAC: MSMAC_1632
METM: MSMTP_2705
MHOR: MSHOH_3505
MTP: Mthe_0102
MCJ: MCON_0570(pscS)
MHI: Mhar_0427
MEMA: MMAB1_1094
MPI: Mpet_1028
MPD: MCP_0857
BARC: AOA65_1061
BARB: AOA66_0045
 » show all
Taxonomy
Reference
1  [PMID:17512006]
  Authors
Fukunaga R, Yokoyama S
  Title
Structural insights into the second step of RNA-dependent cysteine biosynthesis in archaea: crystal structure of Sep-tRNA:Cys-tRNA synthase from Archaeoglobus fulgidus.
  Journal
J Mol Biol 370:128-41 (2007)
DOI:10.1016/j.jmb.2007.04.050
  Sequence
[afu:AF_0028]
Reference
2  [PMID:18559341]
  Authors
Hauenstein SI, Perona JJ
  Title
Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei.
  Journal
J Biol Chem 283:22007-17 (2008)
DOI:10.1074/jbc.M801839200
Reference
3  [PMID:17142313]
  Authors
Yuan J, Palioura S, Salazar JC, Su D, O'Donoghue P, Hohn MJ, Cardoso AM, Whitman WB, Soll D
  Title
RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and  archaea.
  Journal
Proc Natl Acad Sci U S A 103:18923-7 (2006)
DOI:10.1073/pnas.0609703104
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.73
IUBMB Enzyme Nomenclature: 2.5.1.73
ExPASy - ENZYME nomenclature database: 2.5.1.73
BRENDA, the Enzyme Database: 2.5.1.73
CAS: 1239229-21-0

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