KEGG   ENZYME: 2.5.1.87Help
Entry
EC 2.5.1.87                 Enzyme                                 

Name
ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific];
RER2;
Rer2p;
Rer2p Z-prenyltransferase;
Srt1p;
Srt2p Z-prenyltransferase;
ACPT;
dehydrodolichyl diphosphate synthase 1
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
(2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate cistransferase (adding 10--55 isopentenyl units)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate = n diphosphate + ditrans,polycis-polyprenyl diphosphate (n = 10--55) [RN:R05556]
Reaction(KEGG)
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448];
isopentenyl diphosphate [CPD:C00129]
Product
diphosphate [CPD:C00013];
ditrans,polycis-polyprenyl diphosphate [CPD:C05859]
Comment
The enzyme is involved in biosynthesis of dolichol (a long-chain polyprenol) with a saturated alpha-isoprene unit, which serves as a glycosyl carrier in protein glycosylation [1]. The yeast Saccharomyces cerevisiae has two different enzymes that catalyse this reaction. Rer2p synthesizes a well-defined family of polyprenols of 13--18 isoprene residues with dominating C(80) (16 isoprene residues) extending to C(120), while Srt1p synthesizes mainly polyprenol with 22 isoprene subunits. Largest Srt1p products reach C(290) [2]. The enzyme from Arabidopsis thaliana catalyses the formation of polyprenyl diphosphates with predominant carbon number C(120) [4].
History
EC 2.5.1.87 created 2010
Pathway
ec00900  Terpenoid backbone biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K11778  ditrans,polycis-polyprenyl diphosphate synthase
K19177  dehydrodolichyl diphosphate syntase complex subunit NUS1
Genes
HSA: 116150(NUS1) 79947(DHDDS)
PTR: 456662(DHDDS) 462965(NUS1) 741304
PPS: 100972313(DHDDS) 100982942(NUS1) 100988367
GGO: 101125977(DHDDS) 101137725(NUS1)
PON: 100171948(DHDDS) 100460016(NUS1)
NLE: 100602333(NUS1) 100603801(DHDDS)
MCC: 714635(DHDDS) 715015(NUS1)
MCF: 101926129(DHDDS) 102126512(NUS1)
CSAB: 103225956(DHDDS) 103240491(NUS1)
RRO: 104654169(NUS1) 104675752(DHDDS)
RBB: 108518187(DHDDS) 108523008(NUS1)
CJC: 100405741(DHDDS) 100412462(NUS1) 100415318
SBQ: 101028238(NUS1) 101046544(DHDDS)
MMU: 52014(Nus1) 67422(Dhdds)
RNO: 294400(Nus1) 298541(Dhdds)
CGE: 100761045 100761477(Nus1) 100767025(Dhdds)
NGI: 103736296(Nus1) 103737115(Dhdds)
HGL: 101700845(Nus1) 101711794(Dhdds)
CCAN: 109683062(Dhdds) 109693829(Nus1)
OCU: 100339242(NUS1) 100358963(DHDDS)
TUP: 102478223(NUS1) 102482449(DHDDS)
CFA: 484102(NUS1) 612157(DHDDS)
AML: 100463534(DHDDS) 100466277(NUS1)
UMR: 103663529(NUS1) 103666770(DHDDS)
ORO: 101375137(DHDDS) 101380578(NUS1)
FCA: 101085905(DHDDS) 101094180(NUS1)
PTG: 102955679(NUS1) 102966133(DHDDS)
AJU: 106968326(NUS1) 106977611(DHDDS)
BTA: 100849670(NUS1) 523264(DHDDS)
BOM: 102270031(NUS1) 102271246(DHDDS)
BIU: 109563493(NUS1) 109573388(DHDDS)
PHD: 102315363(DHDDS) 102322553(NUS1)
CHX: 102169144(DHDDS) 102173744(NUS1)
OAS: 101102568(NUS1) 101110654(DHDDS)
SSC: 100154658(NUS1) 100524683(DHDDS)
CFR: 102506402(NUS1) 102518547(DHDDS)
CDK: 105088343(NUS1) 105100689(DHDDS)
BACU: 102998703(DHDDS) 103004192(NUS1)
LVE: 103082209(NUS1) 103088197(DHDDS)
OOR: 101271325(NUS1) 101277244(DHDDS)
ECB: 100057242(DHDDS) 100067138(NUS1)
EPZ: 103549504(NUS1) 103550540(DHDDS)
EAI: 106839917(DHDDS) 106847018(NUS1)
MYB: 102238802(DHDDS) 102244874(NUS1)
MYD: 102758520(DHDDS) 102764339(NUS1)
HAI: 109386757(DHDDS) 109396468(NUS1)
RSS: 109449199(DHDDS) 109452447(NUS1)
PALE: 102885564(NUS1) 102891420(DHDDS)
LAV: 100666506(NUS1) 100675778(DHDDS)
MDO: 100012356(DHDDS) 100023437(NUS1)
SHR: 100922631(DHDDS) 100934010(NUS1)
OAA: 100083017 100088240(NUS1)
GGA: 421733(NUS1)
MGP: 100541233(DHDDS) 100550429(NUS1)
CJO: 107311865(NUS1) 107324014(DHDDS)
APLA: 101791531(NUS1) 101804205(DHDDS)
ACYG: 106047572(NUS1) 106047921(DHDDS)
TGU: 100187707(NUS1) 100230447(DHDDS)
GFR: 102038851(DHDDS) 102040482(NUS1)
FAB: 101816671(DHDDS) 101820130(NUS1)
PHI: 102105638(DHDDS) 102108008(NUS1)
PMAJ: 107202140(NUS1) 107214256(DHDDS)
CCAE: 111927186(NUS1) 111939089(DHDDS)
CCW: 104691119(NUS1) 104694634(DHDDS)
FPG: 101919832(DHDDS) 101924551(NUS1)
FCH: 102047823(NUS1) 102049798(DHDDS)
CLV: 102094487(NUS1) 102098335(DHDDS)
EGZ: 104131963(NUS1) 104134887(DHDDS)
AAM: 106485593(DHDDS) 106497998(NUS1)
ASN: 102374803(DHDDS) 102387052(NUS1)
AMJ: 102557872(DHDDS) 102561763(NUS1)
PSS: 102444519(DHDDS) 102445967(NUS1)
CMY: 102936640(NUS1) 102941423(DHDDS)
CPIC: 101946143(NUS1) 101949942(DHDDS)
ACS: 100552810(dhdds) 100553689(nus1)
PVT: 110083607(NUS1) 110086158(DHDDS)
PBI: 103054739(NUS1) 103067643(DHDDS)
GJA: 107108616(NUS1) 107110426(DHDDS)
XLA: 108716803(nus1.L) 108717920(nus1.S) 379162(dhdds.S) 398626(dhdds.L)
XTR: 100135207(dhdds) 548844(nus1)
NPR: 108792824(NUS1) 108800534(DHDDS)
DRE: 406468(dhdds) 436629(nus1)
IPU: 108256361(dhdds) 108257862(nus1)
AMEX: 103039433(nus1) 103040074(dhdds)
TRU: 101067613(dhdds) 101071314(nus1)
LCO: 104924709(dhdds) 104939621(nus1)
MZE: 101465746(dhdds) 101469329(nus1)
OLA: 101156768(dhdds) 101169897(nus1)
XMA: 102217942(nus1) 102232893(dhdds)
PRET: 103458032(nus1) 103477772(dhdds)
NFU: 107379325(dhdds) 107395992(nus1)
KMR: 108228908(nus1) 108240318(dhdds)
CSEM: 103380613(nus1) 103388088(dhdds)
LCF: 108899340(nus1) 108899452(dhdds)
SDU: 111216398(nus1) 111235772(dhdds)
HCQ: 109515435(dhdds) 109516760(nus1)
BPEC: 110156971(nus1) 110174299(dhdds)
MALB: 109954705(dhdds) 109966584(nus1)
ELS: 105017956(nus1) 105028368(dhdds)
SFM: 108919101(dhdds) 108928556(nus1)
LCM: 102351688(NUS1) 102361662(DHDDS)
CMK: 103179564(dhdds) 103182587(nus1)
DME: Dmel_CG10778(CG10778) Dmel_CG4775(Tango14)
DSI: Dsimw501_GD16139(Dsim_GD16139) Dsimw501_GD23075(Dsim_GD23075)
BMOR: 101738415 732991(Dhdds)
API: 100161888 100164640 100574241(ACYPI27127)
CEL: CELE_T01G1.4(T01G1.4)
SHX: MS3_05662
EGL: EGR_09410
ATH: AT1G11755(LEW1) AT2G17570(cPT1) AT2G23400 AT2G23410(CPT) AT5G58770(cPT4) AT5G58780(cPT5) AT5G58782(cPT6) AT5G58784 AT5G60500(cPT8) AT5G60510(cPT9)
LJA: Lj0g3v0314369.1(Lj0g3v0314369.1) Lj0g3v0326269.1(Lj0g3v0326269.1) Lj0g3v0326269.2(Lj0g3v0326269.2) Lj0g3v0326269.3(Lj0g3v0326269.3) Lj0g3v0350299.1(Lj0g3v0350299.1) Lj1g3v0131170.1(Lj1g3v0131170.1) Lj1g3v0131170.2(Lj1g3v0131170.2)
SLY: 101244138(CPT4) 101250227(SlCPTBP) 101253666(CPT3) 101256886(CPT7) 101267417 104644439
DOSA: Os01t0857200-01(Os01g0857200) Os02t0197700-01(Os02g0197700) Os03t0197000-01(Os03g0197000) Os06t0167400-01(Os06g0167400) Os07t0607700-01(Os07g0607700)
ATS: 109742971(LOC109742971) 109757670(LOC109757670) 109757672(LOC109757672) 109757676(LOC109757676) 109759695(LOC109759695) 109762622 109769504 109772847(LOC109772847)
APRO: F751_3077
SCE: YBR002C(RER2) YDL193W(NUS1) YMR101C(SRT1)
KMX: KLMA_30092(SRT1) KLMA_60115(RER2) KLMA_60506(NUS1)
NCS: NCAS_0E01380(NCAS0E01380) NCAS_0E03730(NCAS0E03730) NCAS_0F01440(NCAS0F01440)
NDI: NDAI_0A02900(NDAI0A02900) NDAI_0G01550(NDAI0G01550) NDAI_0H02320(NDAI0H02320)
TPF: TPHA_0A04470(TPHA0A04470) TPHA_0G02950(TPHA0G02950) TPHA_0P00910(TPHA0P00910)
TBL: TBLA_0A05890(TBLA0A05890) TBLA_0B08960(TBLA0B08960) TBLA_0F00550(TBLA0F00550) TBLA_0F02120(TBLA0F02120)
TDL: TDEL_0A02280(TDEL0A02280) TDEL_0C02380(TDEL0C02380) TDEL_0D04190(TDEL0D04190)
KAF: KAFR_0A00610(KAFR0A00610) KAFR_0F02540(KAFR0F02540) KAFR_0L01300(KAFR0L01300)
CAL: CAALFM_C112270WA(SRT1) CAALFM_C304940WA(CaO19.5963) CAALFM_C505330CA(RER2)
SLB: AWJ20_395(RER2) AWJ20_3988(NUS1)
NTE: NEUTE1DRAFT84674(NEUTE1DRAFT_84674) NEUTE1DRAFT91460(NEUTE1DRAFT_91460)
BFU: BCIN_02g03370(Bcnus1) BCIN_05g03890(Bcrer2)
ANG: ANI_1_1896184(An04g05250) ANI_1_888034(An03g06910)
CNE: CND03430
CNB: CNBD2910
ABP: AGABI1DRAFT110974(AGABI1DRAFT_110974) AGABI1DRAFT51492(AGABI1DRAFT_51492)
ABV: AGABI2DRAFT190174(AGABI2DRAFT_190174) AGABI2DRAFT197250(AGABI2DRAFT_197250)
MGL: MGL_1818
PYO: PY17X_0704500(PY05575)
PCB: PCHAS_093380(PC000683.01.0)
TAN: TA04455
TPV: TP03_0421
BBO: BBOV_IV002310(21.m02978)
 » show all
Taxonomy
Reference
1  [PMID:11442630]
  Authors
Sato M, Fujisaki S, Sato K, Nishimura Y, Nakano A
  Title
Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles  in dolichol synthesis.
  Journal
Genes Cells 6:495-506 (2001)
DOI:10.1046/j.1365-2443.2001.00438.x
  Sequence
Reference
2  [PMID:17345630]
  Authors
Poznanski J, Szkopinska A
  Title
Precise bacterial polyprenol length control fails in Saccharomyces cerevisiae.
  Journal
Biopolymers 86:155-64 (2007)
DOI:10.1002/bip.20715
  Sequence
Reference
3  [PMID:9858571]
  Authors
Sato M, Sato K, Nishikawa S, Hirata A, Kato J, Nakano A.
  Title
The yeast RER2 gene, identified by endoplasmic reticulum protein localization mutations, encodes cis-prenyltransferase, a key enzyme in dolichol synthesis.
  Journal
Mol Cell Biol 19:471-83 (1999)
DOI:10.1128/MCB.19.1.471
  Sequence
Reference
4  [PMID:10764783]
  Authors
Oh SK, Han KH, Ryu SB, Kang H
  Title
Molecular cloning, expression, and functional analysis of a cis-prenyltransferase from Arabidopsis thaliana. Implications in rubber biosynthesis.
  Journal
J Biol Chem 275:18482-8 (2000)
DOI:10.1074/jbc.M002000200
  Sequence
[ath:AT2G23410]
Reference
5  [PMID:10908715]
  Authors
Cunillera N, Arro M, Fores O, Manzano D, Ferrer A
  Title
Characterization of dehydrodolichyl diphosphate synthase of Arabidopsis thaliana, a key enzyme in dolichol biosynthesis.
  Journal
FEBS Lett 477:170-4 (2000)
DOI:10.1016/S0014-5793(00)01798-1
  Sequence
[ath:AT2G23410]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.87
IUBMB Enzyme Nomenclature: 2.5.1.87
ExPASy - ENZYME nomenclature database: 2.5.1.87
BRENDA, the Enzyme Database: 2.5.1.87

DBGET integrated database retrieval system