KEGG   ENZYME: 2.5.1.96Help
Entry
EC 2.5.1.96                 Enzyme                                 

Name
4,4'-diapophytoene synthase;
dehydrosqualene synthase;
DAP synthase;
C30 carotene synthase;
CrtM
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
farnesyl-diphosphate:farnesyl-diphosphate farnesyltransferase (15-cis-4,4'-diapophytoene forming)
Reaction(IUBMB)
2 (2E,6E)-farnesyl diphosphate = 15-cis-4,4'-diapophytoene + 2 diphosphate (overall reaction) [RN:R07652];
(1a) 2 (2E,6E)-farnesyl diphosphate = diphosphate + presqualene diphosphate [RN:R00702];
(1b) presqualene diphosphate = 15-cis-4,4'-diapophytoene + diphosphate [RN:R09793]
Reaction(KEGG)
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448];
presqualene diphosphate [CPD:C03428]
Product
15-cis-4,4'-diapophytoene [CPD:C16144];
diphosphate [CPD:C00013];
presqualene diphosphate [CPD:C03428]
Comment
Requires Mn2+. Typical of Staphylococcus aureus and some other bacteria such as Heliobacillus sp.
History
EC 2.5.1.96 created 2011
Pathway
ec00906  Carotenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K10208  4,4'-diapophytoene synthase
Genes
SAU: SA2349(crtM)
SAV: SAV2562(crtM)
SAW: SAHV_2546(crtM)
SAH: SaurJH1_2638
SAJ: SaurJH9_2584
SAM: MW2483(crtM)
SAS: SAS2448
SAC: SACOL2577(crtM)
SAX: USA300HOU_2557(crtM)
SAA: SAUSA300_2499(crtM)
SAE: NWMN_2462(crtM)
SAD: SAAV_2628(crtM)
SUQ: HMPREF0772_10627(crtM)
SUZ: MS7_2569(crtM)
SUX: SAEMRSA15_24650(crtM)
SUW: SATW20_26840(crtM)
SUG: SAPIG2613
SUF: SARLGA251_23370(crtM)
SAUA: SAAG_00383
SAUE: RSAU_002403(crtM)
SAUS: SA40_2316(crtM)
SAUU: SA957_2400(crtM)
SAUG: SA268_2478(crtM)
SAUZ: SAZ172_2662(crtM)
SAUF: X998_2545
SAB: SAB2435c(crtM)
SUY: SA2981_2499(crtM)
SAUB: C248_2620(crtM)
SAUC: CA347_2638(crtM)
SAUR: SABB_03587(crtM)
SAUI: AZ30_13425
SAUD: CH52_06190
SAMS: NI36_12820
SHA: SH0490(crtM)
SCA: SCA_2274
SLN: SLUG_01850(crtM)
SPAS: STP1_1064
SXO: SXYL_00053(crtM)
SCAP: AYP1020_1733(crtM)
 » show all
Taxonomy
Reference
1  [PMID:12426357]
  Authors
Umeno D, Tobias AV, Arnold FH
  Title
Evolution of the C30 carotenoid synthase CrtM for function in a C40 pathway.
  Journal
J Bacteriol 184:6690-9 (2002)
DOI:10.1128/JB.184.23.6690-6699.2002
  Sequence
Reference
2  [PMID:16020541]
  Authors
Pelz A, Wieland KP, Putzbach K, Hentschel P, Albert K, Gotz F.
  Title
Structure and biosynthesis of staphyloxanthin from Staphylococcus aureus.
  Journal
J Biol Chem 280:32493-8 (2005)
DOI:10.1074/jbc.M505070200
  Sequence
[sae:NWMN_2462]
Reference
3  [PMID:16269684]
  Authors
Ku B, Jeong JC, Mijts BN, Schmidt-Dannert C, Dordick JS
  Title
Preparation, characterization, and optimization of an in vitro C30 carotenoid pathway.
  Journal
Appl Environ Microbiol 71:6578-83 (2005)
DOI:10.1128/AEM.71.11.6578-6583.2005
  Sequence
Reference
4  [PMID:18276850]
  Authors
Liu CI, Liu GY, Song Y, Yin F, Hensler ME, Jeng WY, Nizet V, Wang AH, Oldfield E
  Title
A cholesterol biosynthesis inhibitor blocks Staphylococcus aureus virulence.
  Journal
Science 319:1391-4 (2008)
DOI:10.1126/science.1153018
  Sequence
[sau:SA2349]
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.96
IUBMB Enzyme Nomenclature: 2.5.1.96
ExPASy - ENZYME nomenclature database: 2.5.1.96
BRENDA, the Enzyme Database: 2.5.1.96

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