KEGG   ENZYME: 2.6.1.97Help
Entry
EC 2.6.1.97                 Enzyme                                 

Name
archaeosine synthase;
ArcS;
TgtA2;
MJ1022 (gene name);
glutamine:preQ0-tRNA amidinotransferase (incorrect)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
BRITE hierarchy
Sysname
L-glutamine:7-cyano-7-carbaguanine aminotransferase
Reaction(IUBMB)
L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O = L-glutamate + archaeine15 in tRNA
Substrate
L-glutamine [CPD:C00064];
7-cyano-7-carbaguanine15 in tRNA;
H2O [CPD:C00001]
Product
L-glutamate [CPD:C00025];
archaeine15 in tRNA
Comment
In Euryarchaeota the reaction is catalysed by ArcS [1,2]. In Crenarchaeota, which do not have an ArcS homologue, the reaction is catalysed either by a homologue of EC 6.3.4.20, 7-cyano-7-deazaguanine synthase that includes a glutaminase domain (cf. EC 3.5.1.2), or by a homologue of EC 1.7.1.13, preQ1 synthase [2]. The enzyme from the Euryarchaeon Methanocaldococcus jannaschii can also use arginine and ammonium as amino donors.
History
EC 2.6.1.97 created 2012
Orthology
K07557  archaeosine synthase
Genes
MJA: MJ_1022
MFE: Mefer_0631
MVU: Metvu_0618
MFS: MFS40622_1635
MIF: Metin_0871
MJH: JH146_0921
MIG: Metig_0768
MMP: MMP1595
MMQ: MmarC5_1814
MMX: MmarC6_1075
MMD: GYY_08830
MAE: Maeo_1488
MVO: Mvol_1161
MTH: MTH_1665
METC: MTCT_1519
MWO: MWSIV6_0204(arcS1)
METE: tca_01613
MST: Msp_1063
MSI: Msm_0633
MRU: mru_0475
MEB: Abm4_0249
MMIL: sm9_0339(tgtA)
MEYE: TL18_01460
MOL: YLM1_0148
METH: MBMB1_0635
MFC: BRM9_1603
MCUB: MCBB_0612(arcS1)
MFV: Mfer_1055
MKA: MK1084
AFU: AF_0587
FPL: Ferp_1812
GAC: GACE_2024
GAH: GAH_00618
TAC: Ta0924
TVO: TVG1126727(TVG1126727)
PTO: PTO0724
FAI: FAD_0222
CDIV: CPM_0742
MEAR: Mpt1_c07520(arcS)
MARC: AR505_0438
ABI: Aboo_0923
PHO: PH0135(PH0135)
PAB: PAB0082
PFU: PF0071
PFI: PFC_07845
PYN: PNA2_0664
PYS: Py04_0227
TKO: TK2156
TON: TON_1505
TGA: TGAM_2122
TSI: TSIB_1831
THE: GQS_07960
THA: TAM4_168
THM: CL1_1211
TLT: OCC_09014
THS: TES1_1781
TNU: BD01_1969
TEU: TEU_01525
PPAC: PAP_09270
MAC: MA_4632(tgt)
MBAR: MSBR2_0044
MBAK: MSBR3_0025
MMA: MM_1292
MMAC: MSMAC_0023
METM: MSMTP_0025
MTHE: MSTHC_1485
MTHR: MSTHT_1801
MHOR: MSHOH_0023
MBU: Mbur_1772
MMET: MCMEM_0029
MMH: Mmah_1951
MPY: Mpsy_1799
MTP: Mthe_1661
MCJ: MCON_0338
MHI: Mhar_0738
MHU: Mhun_1119
MLA: Mlab_1208
MBG: BN140_2026(tgt1)
MEMA: MMAB1_2626(tgt)
MPI: Mpet_2092
MBN: Mboo_1314
MPL: Mpal_1830
MPD: MCP_1516
MEZ: Mtc_1748
RCI: LRC280(tgt-1)
HAL: VNG_1957G(tgtA2)
HSL: OE_3748R(arcS)
HHB: Hhub_3274(arcS)
HALH: HTSR_1955(tgtA2)
HHSR: HSR6_2030(tgtA2)
HSU: HLASF_1960(tgtA2)
HSF: HLASA_1946(tgtA2)
HMA: rrnAC3261(tgt)
HHI: HAH_0506(tgt2)
NPH: NP_2506A(arcS)
NMO: Nmlp_3874(arcS)
HUT: Huta_2177
HTI: HTIA_2062
HMU: Hmuk_1433
HVO: HVO_2008(arcS)
HME: HFX_2104
HLA: Hlac_2157
HTU: Htur_0714
NMG: Nmag_2683(arcS)
NAT: NJ7G_3957
SALI: L593_08915
IHO: Igni_1353
TPE: Tpen_1279
LOKI: Lokiarch_13630(arcS)
 » show all
Taxonomy
Reference
1  [PMID:20129918]
  Authors
Phillips G, Chikwana VM, Maxwell A, El-Yacoubi B, Swairjo MA, Iwata-Reuyl D, de Crecy-Lagard V
  Title
Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA.
  Journal
J Biol Chem 285:12706-13 (2010)
DOI:10.1074/jbc.M110.102236
  Sequence
[mja:MJ_1022]
Reference
2  [PMID:22032275]
  Authors
Phillips G, Swairjo MA, Gaston KW, Bailly M, Limbach PA, Iwata-Reuyl D, de Crecy-Lagard V
  Title
Diversity of archaeosine synthesis in crenarchaeota.
  Journal
ACS Chem Biol 7:300-5 (2012)
DOI:10.1021/cb200361w
  Sequence
[mja:MJ_1022]
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.97
IUBMB Enzyme Nomenclature: 2.6.1.97
ExPASy - ENZYME nomenclature database: 2.6.1.97
BRENDA, the Enzyme Database: 2.6.1.97

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