KEGG   ENZYME: 2.7.1.160Help
Entry
EC 2.7.1.160                Enzyme                                 

Name
2'-phosphotransferase;
yeast 2'-phosphotransferase;
Tpt1;
Tpt1p;
2'-phospho-tRNA:NAD+ phosphotransferase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
BRITE hierarchy
Sysname
2'-phospho-[ligated tRNA]:NAD+ phosphotransferase
Reaction(IUBMB)
2'-phospho-[ligated tRNA] + NAD+ = mature tRNA + ADP-ribose 1'',2''-phosphate + nicotinamide
Substrate
2'-phospho-[ligated tRNA];
NAD+ [CPD:C00003]
Product
mature tRNA;
ADP-ribose 1'',2''-phosphate [CPD:C19851];
nicotinamide [CPD:C00153]
Comment
Catalyses the final step of tRNA splicing in the yeast Saccharomyces cerevisiae [2]. The reaction takes place in two steps: in the first step, the 2'-phosphate on the RNA substrate is ADP-ribosylated, causing the relase of nicotinamide and the formation of the reaction intermediate, ADP-ribosylated tRNA [6]. In the second step, dephosphorylated (mature) tRNA is formed along with ADP ribose 1''-2''-cyclic phosphate. Highly specific for oligonucleotide substrates bearing an internal 2'-phosphate. Oligonucleotides with only a terminal 5'- or 3'-phosphate are not substrates [1].
History
EC 2.7.1.160 created 2006
Orthology
K10669  2'-phosphotransferase
Genes
HSA: 83707(TRPT1)
PTR: 451288(TRPT1)
PPS: 100985392(TRPT1)
GGO: 101147538(TRPT1)
PON: 100457497(TRPT1)
NLE: 100598276(TRPT1)
MCC: 717911(TRPT1)
MCF: 102130373(TRPT1)
CSAB: 103233411(TRPT1)
RRO: 104673027(TRPT1)
RBB: 108542605(TRPT1)
CJC: 100410369(TRPT1)
SBQ: 101047098(TRPT1)
MMU: 107328(Trpt1)
RNO: 293704(Trpt1)
CGE: 100756285(Trpt1)
NGI: 103738804(Trpt1)
HGL: 101701513(Trpt1)
CCAN: 109683282(Trpt1)
OCU: 100356527(TRPT1)
TUP: 102492696(TRPT1)
CFA: 483765(TRPT1)
AML: 100474877(TRPT1)
UMR: 103679419(TRPT1)
ORO: 101379093(TRPT1)
FCA: 105259622(TRPT1)
PTG: 102962279(TRPT1)
AJU: 106981717(TRPT1)
BTA: 510729(TRPT1)
BOM: 102277874(TRPT1)
BIU: 109554485(TRPT1)
PHD: 102328322(TRPT1)
CHX: 102179356(TRPT1)
OAS: 101122992(TRPT1)
SSC: 100521427(TRPT1)
CFR: 102521618(TRPT1)
CDK: 105101537(TRPT1)
BACU: 103009681(TRPT1)
LVE: 103086666(TRPT1)
OOR: 101271974(TRPT1)
ECB: 100055143(TRPT1)
EPZ: 103542353(TRPT1)
EAI: 106833633(TRPT1)
MYB: 102261357(TRPT1)
MYD: 102773847(TRPT1)
HAI: 109384211(TRPT1)
RSS: 109438194(TRPT1)
PALE: 102880757(TRPT1)
LAV: 100669704(TRPT1)
TMU: 101354027
MDO: 100028474(TRPT1)
SHR: 100921638(TRPT1)
OAA: 100079825(TRPT1)
GGA: 107049849(TRPT1)
MGP: 104915994(TRPT1)
CJO: 107307908(TRPT1)
ASN: 102373360(TRPT1)
AMJ: 102559246(TRPT1)
PSS: 102447359(TRPT1)
CMY: 102932919(TRPT1)
ACS: 100564547(trpt1)
PVT: 110083356(TRPT1)
PBI: 103063200(TRPT1)
GJA: 107115198(TRPT1)
XLA: 108713910(trpt1.L)
XTR: 100216285(trpt1)
NPR: 108794219(TRPT1)
DRE: 503604(trpt1)
IPU: 108268836(trpt1)
AMEX: 103041850(trpt1)
TRU: 101079304(trpt1)
LCO: 104925531(trpt1)
NCC: 104955892(trpt1)
MZE: 101469051(trpt1)
OLA: 101156179(trpt1)
XMA: 102222191(trpt1)
PRET: 103471438(trpt1)
NFU: 107377490(trpt1)
KMR: 108231258(trpt1)
CSEM: 103390823(trpt1)
LCF: 108872565(trpt1)
SDU: 111232368(trpt1)
HCQ: 109515894(trpt1)
BPEC: 110158836(trpt1)
MALB: 109955905(trpt1)
OTW: 112255942(trpt1)
ELS: 105026703(trpt1)
SFM: 108925372(trpt1)
LCM: 102351021(TRPT1)
CIN: 100175594
SPU: 581663
APLC: 110975217
DME: Dmel_CG33057(CG33057)
DSI: Dsimw501_GD12992(Dsim_GD12992)
MDE: 101896994
SOC: 105208010
AEC: 105150949
ACEP: 105619678
PBAR: 105424005
HST: 105191613
DQU: 106746242
CFO: 105251088
LHU: 105668008
PGC: 109853417
NVI: 100123064
MDL: 103574226
TCA: 660925
NVL: 108569228
PMAC: 106710917
PXY: 105392332
FCD: 110861330
SHX: MS3_03942
EPA: 110235733
ADF: 107338159
AQU: 100632909
ATH: AT2G45330(emb1067) AT5G23600
CRB: 17890043
THJ: 104819580
CPAP: 110820906
CIT: 102609292
TCC: 18614316
EGR: 104442248
VRA: 106779437
VAR: 108346356
CCAJ: 109802235
LJA: Lj3g3v2735060.1(Lj3g3v2735060.1)
ADU: 107485037
AIP: 107625947
LANG: 109350416
PPER: 18787573
PMUM: 103331773
PAVI: 110749028
PXB: 103934727
CSV: 101214846
CMO: 103483358
MCHA: 111016108
CMAX: 111472467
CMOS: 111439569
CPEP: 111776504
JCU: 105643132
JRE: 108985810
VVI: 100245349
SLY: 101250158
SPEN: 107008178
SOT: 102600248
CANN: 107873043
NSY: 104247732
INI: 109149063
SIND: 105173884
HAN: 110937752
CCAV: 112526349
DCR: 108210228
BVG: 104905394
SOE: 110803117
OSA: 9267091
DOSA: Os06t0134050-01(Os06g0134050)
OBR: 102709288
BDI: 100838114
ATS: 109764554(LOC109764554) 109769434(LOC109769434) 109784468(LOC109784468)
SBI: 8068396
SITA: 101764864
PDA: 103723773
EGU: 105054773
MUS: 103980623
DCT: 110102207
ATR: 18445875
PPP: 112286316
SCE: YOL102C(TPT1)
ERC: Ecym_3590
KMX: KLMA_80269(TPT1)
NCS: NCAS_0C04770(NCAS0C04770)
NDI: NDAI_0G04120(NDAI0G04120)
TPF: TPHA_0P01070(TPHA0P01070)
TBL: TBLA_0B08270(TBLA0B08270)
TDL: TDEL_0D05360(TDEL0D05360)
KAF: KAFR_0C01470(KAFR0C01470)
CAL: CAALFM_C300350WA(TPT1)
CAUR: QG37_05871
SLB: AWJ20_1517(TPT1)
NCR: NCU06254
NTE: NEUTE1DRAFT62747(NEUTE1DRAFT_62747)
MGR: MGG_12911
SSCK: SPSK_02602
MAW: MAC_03746
MAJ: MAA_05844
CMT: CCM_02606
BFU: BCIN_10g03170(Bctpt1)
MBE: MBM_02383
CIM: CIMG_11231(CIMG03943)
PBN: PADG_11258(PADG_01445)
ABE: ARB_05604
TVE: TRV_07218
PTE: PTT_13014
CNB: CNBE0230
ABP: AGABI1DRAFT100698(AGABI1DRAFT_100698) AGABI1DRAFT70670(AGABI1DRAFT_70670)
ABV: AGABI2DRAFT149451(AGABI2DRAFT_149451) AGABI2DRAFT206430(AGABI2DRAFT_206430)
SMIN: v1.2.004831.t1(symbB.v1.2.004831.t1) v1.2.018195.t3(symbB.v1.2.018195.t3) v1.2.023881.t1(symbB.v1.2.023881.t1) v1.2.032499.t1(symbB.v1.2.032499.t1)
SPAR: SPRG_04019
 » show all
Taxonomy
Reference
1  [PMID:11705403]
  Authors
Steiger MA, Kierzek R, Turner DH, Phizicky EM.
  Title
Substrate recognition by a yeast 2'-phosphotransferase involved in tRNA splicing and by its Escherichia coli homolog.
  Journal
Biochemistry 40:14098-105 (2001)
DOI:10.1021/bi011388t
Reference
2  [PMID:9915792]
  Authors
Spinelli SL, Kierzek R, Turner DH, Phizicky EM.
  Title
Transient ADP-ribosylation of a 2'-phosphate implicated in its removal from ligated tRNA during splicing in yeast.
  Journal
J Biol Chem 274:2637-44 (1999)
DOI:10.1074/jbc.274.5.2637
  Sequence
[sce:YOL102C]
Reference
3  [PMID:9148937]
  Authors
Culver GM, McCraith SM, Consaul SA, Stanford DR, Phizicky EM.
  Title
A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae.
  Journal
J Biol Chem 272:13203-10 (1997)
DOI:10.1074/jbc.272.20.13203
  Sequence
[sce:YOL102C]
Reference
4  [PMID:2050693]
  Authors
McCraith SM, Phizicky EM.
  Title
An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule.
  Journal
J Biol Chem 266:11986-92 (1991)
Reference
5  [PMID:14504659]
  Authors
Hu QD, Lu H, Huo K, Ying K, Li J, Xie Y, Mao Y, Li YY.
  Title
A human homolog of the yeast gene encoding tRNA 2'-phosphotransferase: cloning, characterization and complementation analysis.
  Journal
Cell Mol Life Sci 60:1725-32 (2003)
DOI:10.1007/s00018-003-3107-7
  Sequence
[hsa:83707]
Reference
6  [PMID:15611300]
  Authors
Steiger MA, Jackman JE, Phizicky EM.
  Title
Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.
  Journal
RNA 11:99-106 (2005)
DOI:10.1261/rna.7194605
Reference
7  [PMID:15611301]
  Authors
Sawaya R, Schwer B, Shuman S.
  Title
Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1.
  Journal
RNA 11:107-13 (2005)
DOI:10.1261/rna.7193705
Reference
8  [PMID:15811369]
  Authors
Kato-Murayama M, Bessho Y, Shirouzu M, Yokoyama S.
  Title
Crystal structure of the RNA 2'-phosphotransferase from Aeropyrum pernix K1.
  Journal
J Mol Biol 348:295-305 (2005)
DOI:10.1016/j.jmb.2005.02.049
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.160
IUBMB Enzyme Nomenclature: 2.7.1.160
ExPASy - ENZYME nomenclature database: 2.7.1.160
BRENDA, the Enzyme Database: 2.7.1.160
CAS: 126905-00-8

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