EC                Enzyme                                 

myosin-light-chain kinase;
[myosin-light-chain] kinase;
ATP:myosin-light-chain O-phosphotransferase;
calcium/calmodulin-dependent myosin light chain kinase;
myosin kinase;
myosin light chain kinase;
myosin light chain protein kinase;
myosin light-chain kinase (phosphorylating);
smooth-muscle-myosin-light-chain kinase;
Transferring phosphorus-containing groups;
Protein-serine/threonine kinases
BRITE hierarchy
ATP:[myosin light chain] O-phosphotransferase
ATP + [myosin light chain] = ADP + [myosin light chain] phosphate [RN:R03150]
ATP [CPD:C00002];
myosin light chain [CPD:C01003]
ADP [CPD:C00008];
myosin light chain phosphate [CPD:C03875]
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
EC created 1986 as EC, transferred 2005 to EC
K00907  myosin-light-chain kinase
HSA: 340156(MYLK4) 4638(MYLK) 85366(MYLK2) 91807(MYLK3)
PTR: 101057233(MYLK) 465143(MYLK3) 471840(MYLK4) 742591(MYLK2)
PPS: 100974667(MYLK3) 100978891(MYLK2) 100979422(MYLK) 100995666(MYLK4)
GGO: 101142619(MYLK3) 101143745(MYLK2) 101151517(MYLK) 101151667(MYLK4)
PON: 100171951(MYLK3) 100444714(MYLK4) 100452913(MYLK) 100453783(MYLK2)
NLE: 100585981(MYLK2) 100591151(MYLK) 100594745(MYLK4) 100604194 115831086(MYLK3)
MCC: 707361(MYLK4) 712830(MYLK2) 715422(MYLK) 716132(MYLK3)
MCF: 102122026(MYLK4) 102130711(MYLK) 102132754(MYLK3) 102145766(MYLK2)
CSAB: 103222295(MYLK4) 103228167(MYLK) 103232215(MYLK3) 103248051(MYLK2)
RRO: 104653717(MYLK2) 104655407(MYLK) 104655430(MYLK3) 104661238(MYLK4)
RBB: 108514260(MYLK4) 108517416(MYLK) 108524142(MYLK2) 108534775(MYLK3)
CJC: 100391801(MYLK2) 100392653(MYLK3) 100392656(MYLK4) 100409440(MYLK)
SBQ: 101029220(MYLK) 101038668(MYLK2) 101049056(MYLK4) 101050910(MYLK3) 104653493
MMU: 107589(Mylk) 213435(Mylk3) 228785(Mylk2) 238564(Mylk4)
MCAL: 110290127(Mylk2) 110300419(Mylk3) 110308670(Mylk4) 110311174(Mylk)
MPAH: 110319274(Mylk2) 110329346(Mylk) 110334129(Mylk4) 110337577(Mylk3)
RNO: 100911165(Mylk4) 103689985 117558(Mylk2) 288057(Mylk) 291926(Mylk3)
MUN: 110547228(Mylk) 110550095(Mylk2) 110552995(Mylk3) 110558635(Mylk4)
CGE: 100755963(Mylk2) 100756400(Mylk4) 100758406(Mylk) 100766446(Mylk3)
NGI: 103731078(Mylk2) 103737011(Mylk4) 103741992(Mylk) 103745443(Mylk3)
HGL: 101697894(Mylk3) 101701713(Mylk4) 101704236(Mylk) 101712444(Mylk2)
CCAN: 109677703 109691764(Mylk2) 109700226(Mylk4) 109701077(Mylk) 109703135(Mylk3)
OCU: 100009051(MYLK2) 100009143(MYLK) 100348694(MYLK3) 100353972(MYLK4)
TUP: 102468205(MYLK3) 102476504(MYLK) 102489117(MYLK2) 102498032(MYLK4)
CFA: 475345(MYLK3) 477187(MYLK2) 488012(MYLK) 488190(MYLK4)
VVP: 112907401(MYLK4) 112909931(MYLK2) 112913506(MYLK) 112914947(MYLK3)
AML: 100469244(MYLK2) 100473453(MYLK4) 100474254 100481263(MYLK)
UMR: 103665161(MYLK4) 103673818(MYLK3) 103674343(MYLK2) 103678824(MYLK)
UAH: 113252202(MYLK3) 113252350(MYLK) 113258196(MYLK2) 113264229(MYLK4)
ORO: 101368074(MYLK4) 101370711(MYLK3) 101378973(MYLK) 101381609(MYLK2)
FCA: 101088408(MYLK4) 101091744(MYLK3) 101095813(MYLK2) 494213(MYLK)
PTG: 102951994(MYLK2) 102962607(MYLK3) 102968305(MYLK4) 102972928(MYLK)
PPAD: 109249481(MYLK4) 109266499(MYLK) 109270987(MYLK3) 109272590(MYLK2)
AJU: 106965987(MYLK3) 106976155(MYLK4) 106984088(MYLK) 106986013(MYLK2)
BTA: 338037(MYLK) 518025(MYLK4) 526379(MYLK3) 533378(MYLK2)
BOM: 102264349(MYLK4) 102269080(MYLK) 102275601(MYLK3) 102276600(MYLK2)
BIU: 109555858(MYLK) 109567347(MYLK2) 109572727(MYLK3) 109577322(MYLK4)
BBUB: 102395214(MYLK3) 102402899(MYLK) 102412646(MYLK4) 102415047(MYLK2)
CHX: 102181454(MYLK4) 102181980(MYLK3) 102182147(MYLK2) 102190641(MYLK)
OAS: 101111897(MYLK2) 101113784(MYLK3) 105603910 443334(MYLK)
SSC: 100157526(MYLK4) 100524288(MYLK2) 110260971(MYLK3) 396848(MYLK)
CFR: 102505869(MYLK2) 102511426(MYLK3) 102515628(MYLK4) 102518144(MYLK)
CDK: 105086549(MYLK4) 105090282(MYLK) 105091178(MYLK3) 105100824(MYLK2)
BACU: 103005866(MYLK3) 103008421(MYLK) 103017148(MYLK2) 103020198 103020477(MYLK4)
LVE: 103077707(MYLK4) 103079981(MYLK2) 103083551(MYLK3) 103090456(MYLK)
OOR: 101275794(MYLK4) 101282343(MYLK) 101287286(MYLK3) 101289244(MYLK2)
DLE: 111165019(MYLK3) 111171109 111171669(MYLK) 111181276(MYLK4) 111184432(MYLK2)
PCAD: 102982196(MYLK3) 102982293(MYLK2) 102982405(MYLK) 102984346(MYLK4)
ECB: 100050092(MYLK4) 100053698(MYLK2) 100056559(MYLK3) 100070482(MYLK)
EPZ: 103540989 103547465(MYLK) 103548123(MYLK4) 103559162(MYLK2)
EAI: 106823711(MYLK) 106833720(MYLK2) 106842841(MYLK4) 106848229(MYLK3)
MYB: 102257771(MYLK3) 102258252(MYLK) 102259949(MYLK2) 102262704(MYLK4)
MYD: 102755283(MYLK2) 102759237(MYLK) 102765242(MYLK4) 102769513(MYLK3)
MNA: 107527244(MYLK) 107527759(MYLK2) 107535858(MYLK3) 107542256(MYLK4)
HAI: 109383050(MYLK3) 109384471(MYLK2) 109387190 109388085 109394028(MYLK4)
DRO: 112296778(MYLK3) 112297606(MYLK4) 112303707(MYLK2) 112320512(MYLK)
PALE: 102878549(MYLK3) 102890778(MYLK) 102891799(MYLK2) 102894361(MYLK4)
RAY: 107512268(MYLK3) 107512864(MYLK2) 107516607(MYLK4) 107518034(MYLK)
MJV: 108385316 108393093(MYLK2) 108396719(MYLK) 108402114(MYLK3)
LAV: 100656405(MYLK2) 100665634(MYLK4) 100677183(MYLK)
MDO: 100010869(MYLK2) 100015876(MYLK3) 100019648(MYLK) 100021377(MYLK4)
SHR: 100915288(MYLK2) 100918090(MYLK4) 100925131(MYLK3) 100930073(MYLK)
PCW: 110196726(MYLK4) 110207445(MYLK3) 110208127(MYLK) 110211415(MYLK2)
OAA: 100079091(MYLK4) 100091283(MYLK) 100092442(MYLK3) 114806177(MYLK2)
GGA: 396356(MYLK2) 396445(MYLK) 415748(MYLK3) 420893(MYLK4) 428278(MYLKSML)
MGP: 100538449 100542497(MYLK2) 100543918(MYLK4) 100547989(MYLK) 100551067(MYLK3)
CJO: 107309576(MYLK4) 107316813(MYLK) 107319273(MYLK3) 107323244(MYLK2) 107325036
NMEL: 110388525 110393482(MYLK4) 110400604(MYLK) 110404229(MYLK3) 110408280(MYLK2)
APLA: 101793167(MYLK3) 101794935(MYLK) 101799935(MYLK4) 101800032 101803931(MYLK2)
ACYG: 106029897(MYLK) 106039596(MYLK3) 106046900 106047119(MYLK2) 106048980(MYLK4)
TGU: 100218159(MYLK2) 100222880(MYLK4) 100226590(MYLK) 100232455(MYLK3)
LSR: 110468290(MYLK) 110469015(MYLK2) 110470711(MYLK4) 110475603 110475887(MYLK3)
SCAN: 103814310(MYLK) 103816608(MYLK3) 103818174(MYLK4) 103818839(MYLK2) 103821775
GFR: 102037734(MYLK4) 102037824(MYLK2) 102038683(MYLK3) 102044716(MYLK)
FAB: 101807258(MYLK) 101807804 101809550(MYLK3) 101813259(MYLK2) 101819869(MYLK4)
PHI: 102101059 102104582(MYLK2) 102106790(MYLK) 102111091(MYLK4) 102112254(MYLK3)
PMAJ: 107207555(MYLK) 107209810(MYLK3) 107213081(MYLK2) 107213960(MYLK4) 107215054
CCAE: 111923845(MYLK4) 111932252(MYLK) 111934649(MYLK3) 111938138(MYLK2) 111940063
CCW: 104687441(MYLK3) 104687839(MYLK2) 104694020(MYLK) 104698150(MYLK4) 104698538
ETL: 114058107(MYLK) 114060388(MYLK3) 114063040(MYLK2) 114070606 114071985
FPG: 101910574 101917440(MYLK3) 101922032(MYLK2) 101922066 101922502(MYLK)
FCH: 102050025(MYLK) 102051042(MYLK2) 102051053 102054950(MYLK4) 102058744(MYLK3)
CLV: 102085170 102088141 102093425(MYLK2) 102094050(MYLK4) 102095697(MYLK3)
EGZ: 104122823(MYLK4) 104125557(MYLK3) 104128132(MYLK) 104130019(MYLK2) 104132337
NNI: 104011279(MYLK3) 104018366(MYLK2) 104018798(MYLK) 104018914 104021845(MYLK4)
ACUN: 113476670(MYLK4) 113482370(MYLK) 113484301(MYLK3) 113489147
PADL: 103913791(MYLK3) 103919008(MYLK4) 103919603(MYLK) 103921929 103922199(MYLK2)
AAM: 106482002(MYLK4) 106483357(MYLK3) 106495651 106497378(MYLK2) 106499178(MYLK)
ASN: 102369318(MYLK2) 102371849(MYLK3) 102381743(MYLK) 102384108(MYLK4) 102387857
AMJ: 102557905 102560523(MYLK2) 102561520(MYLK) 102568638(MYLK3) 102574082(MYLK4)
PSS: 102448460(MYLK4) 102458914(MYLK3) 102461334(MYLK2) 102461616(MYLK)
CPIC: 101934934(MYLK4) 101935997(MYLK3) 101945225 101950238(MYLK2) 101951260(MYLK)
ACS: 100557929(mylk) 100559335(mylk3) 100562117(mylk2) 100567803(mylk4)
PVT: 110076324(MYLK3) 110083010(MYLK) 110086708(MYLK2) 110090626(MYLK4)
PMUR: 107284832(MYLK4) 107288928(MYLK) 107290115(MYLK3) 107300163
TSR: 106543664(MYLK) 106555816
PMUA: 114599828(MYLK2) 114600967(MYLK4) 114602252(MYLK3) 114603926(MYLK)
GJA: 107106205 107108885(MYLK3) 107113908(MYLK) 107116916(MYLK2)
XTR: 100489678(mylkl) 100497991(mylk4) 100498081(xkr7) 100498243(mylk3) 101730432(mylk)
NPR: 108785969 108786956(MYLK2) 108787381(MYLK4) 108801018(MYLK) 108802215 108802964(MYLK3)
DRE: 100003770(mylk5) 449707(mylk4b) 492576(mylkb) 558685(mylk2) 561635(mylk3) 566845(mylk4a) 569510(mylka)
SPU: 581963
APLC: 110985015
DME: Dmel_CG42347(sqa)
DER: 6542982
DSI: Dsimw501_GD10055(Dsim_GD10055) Dsimw501_GD25583(Dsim_GD25583)
DSR: 110179249
DPE: 6593039
DMN: 108159863
DWI: 6640809
DAZ: 108617147
DNV: 108653226
DHE: 111592113
MDE: 101900981
LCQ: 111684550
AAG: 5569428
AME: 413190
BIM: 100741566
BTER: 100649174
OBB: 114879979
AEC: 105151728
ACEP: 105618829
PBAR: 105424529
HST: 105190746
DQU: 106748004
CFO: 105256936
LHU: 105670307
PGC: 109858935
OBO: 105284545
PCF: 106790573
NVI: 100118913
MDL: 103570475
TCA: 659444
DPA: 109539715
BMOR: 101741803
BMAN: 114242701
PMAC: 106720800
HAW: 110374133
TNL: 113500367
API: 100163829
DNX: 107173153
AGS: 114121990
BTAB: 109031131
ZNE: 110826815
PVM: 113830372
TUT: 107363672
DPTE: 113799848
CEL: CELE_ZC373.4(mlck-1)
CBR: CBG01967
BMY: Bm1_37415
CRG: 105338083
MYI: 110463894
OBI: 106880214
SHX: MS3_00667
EGL: EGR_05091
EPA: 110232237
PYO: PY17X_1021700(PY07390)
 » show all
1  [PMID:6894756]
Adelstein RS, Klee CB.
Purification and characterization of smooth muscle myosin light chain kinase.
J Biol Chem 256:7501-9 (1981)
2  [PMID:156362]
Hathaway DR, Adelstein RS.
Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity.
Proc Natl Acad Sci U S A 76:1653-7 (1979)
3  [PMID:4853304]
Pires E, Perry SV, Thomas MA.
Myosin light-chain kinase, a new enzyme from striated muscle.
FEBS Lett 41:292-6 (1974)
4  [PMID:3881420]
Nunnally MH, Rybicki SB, Stull JT.
Characterization of chicken skeletal muscle myosin light chain kinase. Evidence for muscle-specific isozymes.
J Biol Chem 260:1020-6 (1985)
5  [PMID:3897230]
Edelman AM, Takio K, Blumenthal DK, Hansen RS, Walsh KA, Titani K, Krebs EG.
Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.
J Biol Chem 260:11275-85 (1985)
6  [PMID:12390014]
Mal TK, Skrynnikov NR, Yap KL, Kay LE, Ikura M.
Detecting protein kinase recognition modes of calmodulin by residual dipolar couplings in solution NMR.
Biochemistry 41:12899-906 (2002)
7  [PMID:10231558]
Sobieszek A.
Enzyme kinetic characterization of the smooth muscle myosin phosphorylating system: activation by calcium and calmodulin and possible inhibitory mechanisms of antagonists.
Biochim Biophys Acta 1450:77-91 (1999)
8  [PMID:9054394]
Sobieszek A, Borkowski J, Babiychuk VS.
Purification and characterization of a smooth muscle myosin light chain kinase-phosphatase complex.
J Biol Chem 272:7034-41 (1997)
9  [PMID:10098974]
Fujita K, Ye LH, Sato M, Okagaki T, Nagamachi Y, Kohama K.
Myosin light chain kinase from skeletal muscle regulates an ATP-dependent interaction between actin and myosin by binding to actin.
Mol Cell Biochem 190:85-90 (1999)
Other DBs
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IUBMB Enzyme Nomenclature:
ExPASy - ENZYME nomenclature database:
BRENDA, the Enzyme Database:
CAS: 51845-53-5

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