KEGG   ENZYME: 2.7.11.18Help
Entry
EC 2.7.11.18                Enzyme                                 

Name
myosin-light-chain kinase;
[myosin-light-chain] kinase;
ATP:myosin-light-chain O-phosphotransferase;
calcium/calmodulin-dependent myosin light chain kinase;
MLCK;
MLCKase;
myosin kinase;
myosin light chain kinase;
myosin light chain protein kinase;
myosin light-chain kinase (phosphorylating);
smooth-muscle-myosin-light-chain kinase;
STK18
Class
Transferases;
Transferring phosphorus-containing groups;
Protein-serine/threonine kinases
BRITE hierarchy
Sysname
ATP:[myosin light chain] O-phosphotransferase
Reaction(IUBMB)
ATP + [myosin light chain] = ADP + [myosin light chain] phosphate [RN:R03150]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
myosin light chain [CPD:C01003]
Product
ADP [CPD:C00008];
myosin light chain phosphate [CPD:C03875]
Comment
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
History
EC 2.7.11.18 created 1986 as EC 2.7.1.117, transferred 2005 to EC 2.7.11.18
Orthology
K00907  myosin-light-chain kinase
Genes
HSA: 340156(MYLK4) 4638(MYLK) 85366(MYLK2) 91807(MYLK3)
PTR: 101057233(MYLK) 465143(MYLK3) 471840(MYLK4) 742591(MYLK2)
PPS: 100974667(MYLK3) 100978891(MYLK2) 100979422(MYLK) 100995666(MYLK4)
GGO: 101142619(MYLK3) 101143745(MYLK2) 101151517(MYLK) 101151667(MYLK4)
PON: 100171951(MYLK3) 100444714(MYLK4) 100452913(MYLK) 100453783(MYLK2)
NLE: 100585981(MYLK2) 100591151(MYLK) 100594745(MYLK4) 100604194(MYLK3)
MCC: 707361(MYLK4) 712830(MYLK2) 715422(MYLK) 716132(MYLK3)
MCF: 102122026(MYLK4) 102130711(MYLK) 102132754(MYLK3) 102145766(MYLK2)
CSAB: 103222295(MYLK4) 103228167(MYLK) 103232215(MYLK3) 103248051(MYLK2)
RRO: 104653717(MYLK2) 104655407(MYLK) 104655430(MYLK3) 104661238(MYLK4)
RBB: 108514260(MYLK4) 108517416(MYLK) 108524142(MYLK2) 108534775(MYLK3)
CJC: 100391801(MYLK2) 100392653(MYLK3) 100392656(MYLK4) 100409440(MYLK)
SBQ: 101029220(MYLK) 101038668(MYLK2) 101049056(MYLK4) 101050910(MYLK3) 104653493
MMU: 107589(Mylk) 213435(Mylk3) 228785(Mylk2) 238564(Mylk4)
RNO: 100911165(Mylk4) 103689985 117558(Mylk2) 288057(Mylk) 291926(Mylk3)
CGE: 100755963(Mylk2) 100756400(Mylk4) 100758406(Mylk) 100766446(Mylk3)
NGI: 103731078(Mylk2) 103737011(Mylk4) 103741992(Mylk) 103745443(Mylk3)
HGL: 101697894(Mylk3) 101701713(Mylk4) 101704236(Mylk) 101712444(Mylk2)
CCAN: 109677703 109691764(Mylk2) 109700226(Mylk4) 109701077(Mylk) 109703135(Mylk3)
OCU: 100009051(MYLK2) 100009143(MYLK) 100348694(MYLK3) 100353972(MYLK4)
TUP: 102468205(MYLK3) 102476504(MYLK) 102489117(MYLK2) 102498032(MYLK4)
CFA: 475345(MYLK3) 477187(MYLK2) 488012(MYLK) 488190(MYLK4)
AML: 100469244(MYLK2) 100473453(MYLK4) 100474254 100481263(MYLK)
UMR: 103665161(MYLK4) 103673818(MYLK3) 103674343(MYLK2) 103678824(MYLK)
ORO: 101368074(MYLK4) 101370711(MYLK3) 101378973(MYLK) 101381609(MYLK2)
FCA: 101088408(MYLK4) 101091744(MYLK3) 101095813(MYLK2) 494213(MYLK)
PTG: 102951994(MYLK2) 102962607(MYLK3) 102968305(MYLK4) 102972928(MYLK)
AJU: 106965987(MYLK3) 106976155(MYLK4) 106984088(MYLK) 106986013(MYLK2)
BTA: 338037(MYLK) 518025(MYLK4) 526379(MYLK3) 533378(MYLK2)
BOM: 102264349(MYLK4) 102269080(MYLK) 102275601(MYLK3) 102276600(MYLK2)
BIU: 109555858(MYLK) 109567347(MYLK2) 109572727(MYLK3) 109577322(MYLK4)
PHD: 102331525(MYLK2) 102337475(MYLK) 102337545(MYLK3)
CHX: 102181454(MYLK4) 102181980(MYLK3) 102182147(MYLK2) 102190641(MYLK)
OAS: 101111897(MYLK2) 101113784(MYLK3) 105603910(MYLK4) 443334(MYLK)
SSC: 100157526(MYLK4) 100524288(MYLK2) 110260971(MYLK3) 396848(MYLK)
CFR: 102505869(MYLK2) 102511426(MYLK3) 102515628(MYLK4) 102518144(MYLK)
CDK: 105086549(MYLK4) 105090282(MYLK) 105091178(MYLK3) 105100824(MYLK2)
BACU: 103005866(MYLK3) 103008421(MYLK) 103017148(MYLK2) 103020198 103020477(MYLK4)
LVE: 103077707(MYLK4) 103079981(MYLK2) 103083551(MYLK3) 103090456(MYLK)
OOR: 101275794(MYLK4) 101282343(MYLK) 101287286(MYLK3) 101289244(MYLK2)
ECB: 100050092(MYLK4) 100053698(MYLK2) 100056559(MYLK3) 100070482(MYLK)
EPZ: 103540564 103540989 103547465(MYLK) 103548123(MYLK4) 103559162(MYLK2)
EAI: 106823711(MYLK) 106833720(MYLK2) 106842841(MYLK4) 106848229(MYLK3)
MYB: 102257771(MYLK3) 102258252(MYLK) 102259949(MYLK2) 102262704(MYLK4)
MYD: 102755283(MYLK2) 102759237(MYLK) 102765242(MYLK4) 102769513(MYLK3)
HAI: 109383050(MYLK3) 109384471(MYLK2) 109387190 109388085 109394028(MYLK4)
RSS: 109447452(MYLK3) 109451058(MYLK) 109456288(MYLK2) 109458606(MYLK4)
PALE: 102878549(MYLK3) 102890778(MYLK) 102891799(MYLK2) 102894361(MYLK4)
LAV: 100656405(MYLK2) 100665634(MYLK4) 100677183(MYLK)
MDO: 100010869(MYLK2) 100015876(MYLK3) 100019648(MYLK) 100021377(MYLK4)
SHR: 100915288(MYLK2) 100918090(MYLK4) 100925131(MYLK3) 100930073(MYLK)
OAA: 100079091(MYLK4) 100091283 100092442(MYLK3)
GGA: 396356(MYLK2) 396445(MYLK) 415748(MYLK3) 420893(MYLK4) 428278(MYLKSML)
MGP: 100538449 100542497(MYLK2) 100543918(MYLK4) 100547989(MYLK) 100551067(MYLK3)
CJO: 107309576(MYLK4) 107316813(MYLK) 107319273(MYLK3) 107323244(MYLK2) 107325036
APLA: 101793167(MYLK3) 101794935(MYLK) 101799935(MYLK4) 101800032 101803931(MYLK2)
ACYG: 106029897(MYLK) 106039596(MYLK3) 106046900 106047119(MYLK2) 106048980(MYLK4)
TGU: 100222880(MYLK4) 100226590(MYLK) 100232455(MYLK3)
GFR: 102037734(MYLK4) 102037824(MYLK2) 102038683(MYLK3) 102044716(MYLK)
FAB: 101807258(MYLK) 101807804 101809550(MYLK3) 101813259(MYLK2) 101819869(MYLK4)
PHI: 102101059 102104582(MYLK2) 102106790(MYLK) 102111091(MYLK4) 102112254(MYLK3)
PMAJ: 107207555(MYLK) 107209810(MYLK3) 107213081(MYLK2) 107213960(MYLK4) 107215054
CCAE: 111923845(MYLK4) 111932252(MYLK) 111934649(MYLK3) 111938138(MYLK2) 111940063
CCW: 104687441(MYLK3) 104687839(MYLK2) 104694020(MYLK) 104698150(MYLK4) 104698538
FPG: 101910574 101917440(MYLK3) 101922032(MYLK2) 101922066 101922502(MYLK)
FCH: 102050025(MYLK) 102051042(MYLK2) 102051053 102054950(MYLK4) 102058744(MYLK3)
CLV: 102085170 102088141 102093425(MYLK2) 102094050(MYLK4) 102095697(MYLK3)
EGZ: 104122823(MYLK4) 104125557(MYLK3) 104128132(MYLK) 104130019(MYLK2) 104132337
AAM: 106482002(MYLK4) 106483357(MYLK3) 106495651 106497378(MYLK2) 106499178(MYLK)
ASN: 102369318(MYLK2) 102371849(MYLK3) 102381743(MYLK) 102384108(MYLK4) 102387857
AMJ: 102557905 102560523(MYLK2) 102561520(MYLK) 102568638(MYLK3) 102574082(MYLK4)
PSS: 102448460(MYLK4) 102458914(MYLK3) 102461334(MYLK2) 102461616(MYLK)
CPIC: 101934934(MYLK4) 101935997(MYLK3) 101945225 101950238(MYLK2) 101951260(MYLK)
ACS: 100557929(mylk) 100559335(mylk3) 100562117(mylk2) 100567803(mylk4)
PVT: 110076324(MYLK3) 110083010(MYLK) 110086708(MYLK2) 110090626(MYLK4)
GJA: 107106205 107108885(MYLK3) 107113908(MYLK) 107116916(MYLK2)
XTR: 100489678(mylk) 100497991(mylk4) 100498081(xkr7) 100498243(mylk3) 101730432
NPR: 108785969 108786956(MYLK2) 108787381(MYLK4) 108801018(MYLK) 108802215 108802964(MYLK3)
DRE: 100003770(mylk5) 449707(mylk4b) 492576(mylkb) 558685(mylk2) 561635(mylk3) 566845(mylk4a) 569510(mylka)
SPU: 581963
APLC: 110985015
DME: Dmel_CG42347(sqa)
DSI: Dsimw501_GD10055(Dsim_GD10055) Dsimw501_GD25583(Dsim_GD25583)
MDE: 101900981
AAG: 5569428
AME: 413190
BIM: 100741566
BTER: 100649174
AEC: 105151728
ACEP: 105618829
PBAR: 105424529
HST: 105190746
DQU: 106748004
CFO: 105256936
LHU: 105670307
PGC: 109858935
PCF: 106790573
NVI: 100118913
MDL: 103570475
TCA: 659444
DPA: 109539715
BMOR: 101741803
PMAC: 106720800
HAW: 110374133
API: 100163829
DNX: 107173153
ZNE: 110826815
TUT: 107363672
CEL: CELE_ZC373.4(mlck-1)
CBR: CBG01967
BMY: Bm1_37415
CRG: 105338083
MYI: 110463894
OBI: 106880214
SHX: MS3_00667
EGL: EGR_05091
EPA: 110232237
PYO: PY17X_1021700(PY07390)
 » show all
Taxonomy
Reference
1  [PMID:6894756]
  Authors
Adelstein RS, Klee CB.
  Title
Purification and characterization of smooth muscle myosin light chain kinase.
  Journal
J Biol Chem 256:7501-9 (1981)
Reference
2  [PMID:156362]
  Authors
Hathaway DR, Adelstein RS.
  Title
Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity.
  Journal
Proc Natl Acad Sci U S A 76:1653-7 (1979)
DOI:10.1073/pnas.76.4.1653
Reference
3  [PMID:4853304]
  Authors
Pires E, Perry SV, Thomas MA.
  Title
Myosin light-chain kinase, a new enzyme from striated muscle.
  Journal
FEBS Lett 41:292-6 (1974)
DOI:10.1016/0014-5793(74)81232-9
Reference
4  [PMID:3881420]
  Authors
Nunnally MH, Rybicki SB, Stull JT.
  Title
Characterization of chicken skeletal muscle myosin light chain kinase. Evidence for muscle-specific isozymes.
  Journal
J Biol Chem 260:1020-6 (1985)
Reference
5  [PMID:3897230]
  Authors
Edelman AM, Takio K, Blumenthal DK, Hansen RS, Walsh KA, Titani K, Krebs EG.
  Title
Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.
  Journal
J Biol Chem 260:11275-85 (1985)
Reference
6  [PMID:12390014]
  Authors
Mal TK, Skrynnikov NR, Yap KL, Kay LE, Ikura M.
  Title
Detecting protein kinase recognition modes of calmodulin by residual dipolar couplings in solution NMR.
  Journal
Biochemistry 41:12899-906 (2002)
DOI:10.1021/bi0264162
Reference
7  [PMID:10231558]
  Authors
Sobieszek A.
  Title
Enzyme kinetic characterization of the smooth muscle myosin phosphorylating system: activation by calcium and calmodulin and possible inhibitory mechanisms of antagonists.
  Journal
Biochim Biophys Acta 1450:77-91 (1999)
Reference
8  [PMID:9054394]
  Authors
Sobieszek A, Borkowski J, Babiychuk VS.
  Title
Purification and characterization of a smooth muscle myosin light chain kinase-phosphatase complex.
  Journal
J Biol Chem 272:7034-41 (1997)
DOI:10.1074/jbc.272.11.7034
Reference
9  [PMID:10098974]
  Authors
Fujita K, Ye LH, Sato M, Okagaki T, Nagamachi Y, Kohama K.
  Title
Myosin light chain kinase from skeletal muscle regulates an ATP-dependent interaction between actin and myosin by binding to actin.
  Journal
Mol Cell Biochem 190:85-90 (1999)
Other DBs
ExplorEnz - The Enzyme Database: 2.7.11.18
IUBMB Enzyme Nomenclature: 2.7.11.18
ExPASy - ENZYME nomenclature database: 2.7.11.18
BRENDA, the Enzyme Database: 2.7.11.18
CAS: 51845-53-5

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