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Entry
EC 2.7.7.69                 Enzyme                                 

Name
GDP-L-galactose phosphorylase;
VTC2;
VTC5
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
BRITE hierarchy
Sysname
GDP:alpha-L-galactose 1-phosphate guanylyltransferase
Reaction(IUBMB)
GDP-beta-L-galactose + phosphate = beta-L-galactose 1-phosphate + GDP
Reaction(KEGG)
(other) R07678
Show
Substrate
GDP-beta-L-galactose;
phosphate [CPD:C00009]
Product
beta-L-galactose 1-phosphate [CPD:C15926];
GDP [CPD:C00035]
Comment
The enzyme catalyses a reaction of the Smirnoff-Wheeler pathway, the major route to ascorbate biosynthesis in plants.
History
EC 2.7.7.69 created 2010
Pathway
ec00053  Ascorbate and aldarate metabolism
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K14190  GDP-L-galactose phosphorylase
Genes
RSS: 109440458 109440708
ATH: AT4G26850(VTC2) AT5G55120(VTC5)
ALY: ARALYDRAFT_492115 ARALYDRAFT_655221 ARALYDRAFT_918651
CRB: 17875360 17878615
CSAT: 104717545 104722245 104726045 104730704 104731426 104761532
EUS: EUTSA_v10013622mg EUTSA_v10025206mg
BRP: 103834830 103851903 103861660 103866673(VTC2)
BNA: 106361354 106418494 106420809 106421390 106429510 106444877 106445470
BOE: 106308684 106312359 106327758
THJ: 104811624 104813430
CPAP: 110820751
LJA: Lj0g3v0129419.1(Lj0g3v0129419.1) Lj0g3v0268149.1(Lj0g3v0268149.1)
CSV: 101205337
CMO: 103490128
SLY: 101055572(GGP) 101255942(SlGGP-LIKE)
DOSA: Os12t0190000-01(Os12g0190000) Os12t0612100-01(Os12g0612100)
ATS: 109736377(LOC109736377) 109741548(LOC109741548) 109780373(LOC109780373) 109781055(LOC109781055)
ZMA: 100272546(si946084h12) 100283852
MNG: MNEG_9746
APRO: F751_3621
 » show all
Taxonomy
Reference
1  [PMID:17462988]
  Authors
Linster CL, Gomez TA, Christensen KC, Adler LN, Young BD, Brenner C, Clarke SG.
  Title
Arabidopsis VTC2 Encodes a GDP-L-Galactose Phosphorylase, the Last Unknown Enzyme in the Smirnoff-Wheeler Pathway to Ascorbic Acid in Plants.
  Journal
J Biol Chem 282:18879-85 (2007)
DOI:10.1074/jbc.M702094200
  Sequence
[ath:AT4G26850]
Reference
2  [PMID:18463094]
  Authors
Linster CL, Adler LN, Webb K, Christensen KC, Brenner C, Clarke SG
  Title
A second GDP-L-galactose phosphorylase in arabidopsis en route to vitamin C. Covalent intermediate and substrate requirements for the conserved reaction.
  Journal
J Biol Chem 283:18483-92 (2008)
DOI:10.1074/jbc.M802594200
  Sequence
Reference
3  [PMID:17877701]
  Authors
Dowdle J, Ishikawa T, Gatzek S, Rolinski S, Smirnoff N
  Title
Two genes in Arabidopsis thaliana encoding GDP-L-galactose phosphorylase are required for ascorbate biosynthesis and seedling viability.
  Journal
Plant J 52:673-89 (2007)
DOI:10.1111/j.1365-313X.2007.03266.x
  Sequence
Reference
4  [PMID:18516687]
  Authors
Muller-Moule P
  Title
An expression analysis of the ascorbate biosynthesis enzyme VTC2.
  Journal
Plant Mol Biol 68:31-41 (2008)
DOI:10.1007/s11103-008-9350-4
  Sequence
[ath:AT4G26850]
Other DBs
ExplorEnz - The Enzyme Database: 2.7.7.69
IUBMB Enzyme Nomenclature: 2.7.7.69
ExPASy - ENZYME nomenclature database: 2.7.7.69
BRENDA, the Enzyme Database: 2.7.7.69

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