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Entry
EC 2.8.2.22                 Enzyme                                 

Name
aryl-sulfate sulfotransferase;
arylsulfate-phenol sulfotransferase;
arylsulfotransferase;
ASST;
arylsulfate sulfotransferase;
arylsulfate:phenol sulfotransferase;
astA (gene name)
Class
Transferases;
Transferring sulfur-containing groups;
Sulfotransferases
BRITE hierarchy
Sysname
aryl-sulfate:phenol sulfotransferase
Reaction(IUBMB)
an aryl sulfate + a phenol = a phenol + an aryl sulfate
Reaction(KEGG)
(other) R02996
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Substrate
aryl sulfate [CPD:C00850];
phenol [CPD:C15584]
Product
phenol [CPD:C15584];
aryl sulfate [CPD:C00850]
Comment
The enzyme, characterized from bacteria that colonize the human and mouse intestine, catalyses the transfer of a sulfate group from a phenol sulfate ester to other phenolic compounds. Activity is enhanced by Mg2+ and Mn2+ [1]. Unlike EC 2.8.2.9, tyrosine-ester sulfotransferase and EC 2.8.2.1, aryl sulfotransferase, the enzyme does not act on 3'-phosphoadenylyl sulfate or adenosine 3',5'-bisphosphate [1].
The level of sulfation of polyphenols depends on the positions of the hydroxyl groups [3-5]. Hydroxy groups of tyrosine residues in peptides such as angiotensin can also act as acceptors [2]. The reaction proceeds according to a ping pong bi bi mechanism [6].
History
EC 2.8.2.22 created 1990
Orthology
K01023  arylsulfate sulfotransferase
Genes
ECG: E2348C_3334
ECC: c3785(asst)
ECP: ECP_3130
ECI: UTI89_C3473(asst)
ECV: APECO1_3375(assT)
ECM: EcSMS35_3333
ECQ: ECED1_3704(assT)
EUM: ECUMN_3523(assT)
ECZ: ECS88_3432(assT)
ELO: EC042_3329(assT)
ESE: ECSF_2880
ENA: ECNA114_3129(asst)
ELC: i14_3479(asst)
ELD: i02_3479(asst)
ELF: LF82_506
ECOJ: P423_17180
ECOS: EC958_3441(assT)
EFE: EFER_2980(assT)
STY: STY3370(assT)
STT: t3112(assT)
STM: STM4098
SEY: SL1344_3165(assT)
SEF: UMN798_3471(assT)
SENR: STMDT2_30851(assT)
SENI: CY43_16635
SPT: SPA3060(assT)
SEK: SSPA2856
SEI: SPC_3266(assT)
SEC: SCH_3136(astJ)
SHB: SU5_089
SENS: Q786_15545
SED: SeD_A3545
SEG: SG3088
SEL: SPUL_3203
SET: SEN3034
SENA: AU38_15435
SENO: AU37_15440
SENV: AU39_15440
SENQ: AU40_17395
SENL: IY59_16040
SEEP: I137_15285
SENE: IA1_15430
ECLO: ENC_41780
ECLZ: LI64_00125
CRO: ROD_35871(assT)
ETR: ETAE_0524(assT)
ETD: ETAF_0475
ETE: ETEE_2284
PPUH: B479_01490
PPUT: L483_00795
PPUN: PP4_02300
PEN: PSEEN0187
PSEC: CCOS191_0184(assT)
SON: SO_3872(assT)
SPL: Spea_4022
SHL: Shal_0236
SWD: Swoo_1752
SWP: swp_4147
FBL: Fbal_0078
AMED: B224_6011
RGE: RGE_17750
CJU: C8J_0813
CJM: CJM1_0839(astA) CJM1_0840
CJEJ: N564_02065
CJEU: N565_02155
CJEN: N755_02180
CJY: QZ67_00935(assT)
CJQ: UC78_0827(assT_1) UC78_0828(assT_2)
CFT: CFF04554_0434(astA)
CFV: CFVI03293_0433(astA)
CFX: CFV97608_0436(astA)
CFZ: CSG_5150
CAMP: CFT03427_0445(astA)
CCV: CCV52592_0844(astA1) CCV52592_1149(astA3)
CCO: CCC13826_0901(astA2)
CCOC: CCON33237_0098(astA1) CCON33237_1797(astA2)
CLA: Cla_0560(astA)
CLR: UPTC16701_0560(astA)
CLM: UPTC16712_0566(astA)
CLQ: UPTC4110_0568(astA)
CLN: UPTC3659_0594(astA1) UPTC3659_0595(astA2)
CLL: CONCH_0566(astA)
CIS: CINS_0482(astA)
CVO: CVOL_0497(astA)
CPEL: CPEL_0515(astA)
CAMR: CAQ16704_0592(astA1) CAQ16704_0593(astA2)
CSM: CSUB8521_0597(astA)
CSF: CSUB8523_0584(astA)
CGRA: CGRAC_2066 CGRAC_2074(assT)
CSPF: CSF_0054(astA1) CSF_0055(astA2) CSF_0056(astA3) CSF_0057(astA4) CSF_0468(astA5)
CPIN: CPIN18020_0377(astA1) CPIN18020_0597(astA2)
SMUL: SMUL_0054(assT)
SHAL: SHALO_0031
GME: Gmet_1382
GEO: Geob_2732
GEM: GM21_1436
BEO: BEH_12040
LSG: lse_1957(astA)
LIV: LIV_1951
LPL: lp_1381(astA)
LPJ: JDM1_1159(astA)
LPT: zj316_1433(astA)
LPS: LPST_C1109(astA)
LPZ: Lp16_1060
LBR: LVIS_2143
LHD: HUO_04080
BPRL: CL2_18210
MBJ: KQ51_01780(assT)
SIJ: SCIP_0304
PBU: L21SP3_01066(assT)
PBP: STSP1_01607(assT)
BPW: WESB_2441
BIP: Bint_2909
ABAS: ACPOL_3964
BVU: BVU_0583
 » show all
Taxonomy
Reference
1  [PMID:3460636]
  Authors
Kim DH, Konishi L, Kobashi K.
  Title
Purification, characterization and reaction mechanism of novel arylsulfotransferase obtained from an anaerobic bacterium of human intestine.
  Journal
Biochim Biophys Acta 872:33-41 (1986)
DOI:10.1016/0167-4838(86)90144-5
Reference
2
  Authors
Kobashi, K., Kim, D.-H. and Morikawa, T.
  Title
A novel type of arylsulfotransferase.
  Journal
J Protein Chem 6:237-244 (1987)
Reference
3  [PMID:2347024]
  Authors
Koizumi M, Shimizu M, Kobashi K
  Title
Enzymatic sulfation of quercetin by arylsulfotransferase from a human intestinal  bacterium.
  Journal
Chem Pharm Bull (Tokyo) 38:794-6 (1990)
Reference
4  [PMID:1806284]
  Authors
Koizumi M, Akao T, Kadota S, Kikuchi T, Okuda T, Kobashi K
  Title
Enzymatic sulfation of polyphenols related to tannins by arylsulfotransferase.
  Journal
Chem Pharm Bull (Tokyo) 39:2638-43 (1991)
Reference
5  [PMID:7820100]
  Authors
Konishi-Imamura L, Sato M, Dohi K, Kadota S, Namba T, Kobashi K
  Title
Enzymatic sulfation of glycosides and their corresponding aglycones by arylsulfate sulfotransferase from a human intestinal bacterium.
  Journal
Biol Pharm Bull 17:1018-22 (1994)
Reference
6  [PMID:8576095]
  Authors
Lee NS, Kim BT, Kim DH, Kobashi K
  Title
Purification and reaction mechanism of arylsulfate sulfotransferase from Haemophilus K-12, a mouse intestinal bacterium.
  Journal
J Biochem 118:796-801 (1995)
Reference
7  [PMID:17202651]
  Authors
Kim B, Hyun YJ, Lee KS, Kobashi K, Kim DH
  Title
Cloning, expression and purification of arylsulfate sulfotransferase from Eubacterium A-44.
  Journal
Biol Pharm Bull 30:11-4 (2007)
Other DBs
ExplorEnz - The Enzyme Database: 2.8.2.22
IUBMB Enzyme Nomenclature: 2.8.2.22
ExPASy - ENZYME nomenclature database: 2.8.2.22
BRENDA, the Enzyme Database: 2.8.2.22
CAS: 158254-86-5

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