KEGG   ENZYME: 2.8.2.23Help
Entry
EC 2.8.2.23                 Enzyme                                 

Name
[heparan sulfate]-glucosamine 3-sulfotransferase 1;
heparin-glucosamine 3-O-sulfotransferase;
3'-phosphoadenylyl-sulfate:heparin-glucosamine 3-O-sulfotransferase;
glucosaminyl 3-O-sulfotransferase;
heparan sulfate D-glucosaminyl 3-O-sulfotransferase;
isoform/isozyme 1 (3-OST-1, HS3ST1)
Class
Transferases;
Transferring sulfur-containing groups;
Sulfotransferases
BRITE hierarchy
Sysname
3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfotransferase
Reaction(IUBMB)
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate [RN:R05798]
Reaction(KEGG)
Substrate
3'-phosphoadenylyl sulfate [CPD:C00053];
[heparan sulfate]-glucosamine [CPD:C17141]
Product
adenosine 3',5'-bisphosphate [CPD:C00054];
[heparan sulfate]-glucosamine 3-sulfate [CPD:C11553]
Comment
This enzyme differs from the other [heparan sulfate]-glucosamine 3-sulfotransferases [EC 2.8.2.29 ([heparan sulfate]-glucosamine 3-sulfotransferase 2) and EC 2.8.2.30 ([heparan sulfate]-glucosamine 3-sulfotransferase 3)] by being the most selective for a precursor of the antithrombin-binding site. It has a minimal acceptor sequence of: -> GlcNAc6S-> GlcA-> GlcN2S*+/-6S-> IdoA2S-> GlcN2S-> , the asterisk marking the target (symbols as in {iupac/2carb/38::2-Carb-38}) using +/- to mean the presence or absence of a substituent, and > to separate a predominant structure from a minor one. Thus Glc(N2S > NAc) means a residue of glucosamine where the N carries a sulfo group mainly but occasionally an acetyl group.) [1-4]. It can also modify other precursor sequences within heparan sulfate but this action does not create functional antithrombin-binding sites. These precursors are variants of the consensus sequence: -> Glc(N2S > NAc)+/-6S-> GlcA-> GlcN2S*+/-6S-> GlcA > IdoA+/-2S-> Glc(N2S/NAc)+/-6S-> [5]. If the heparan sulfate substrate lacks 2-O-sulfation of GlcA residues, then enzyme specificity is expanded to modify selected glucosamine residues preceded by IdoA as well as GlcA [6].
History
EC 2.8.2.23 created 1992, modified 2001
Pathway
ec00534  Glycosaminoglycan biosynthesis - heparan sulfate / heparin
Orthology
K01024  [heparan sulfate]-glucosamine 3-sulfotransferase 1
K08104  [heparan sulfate]-glucosamine 3-sulfotransferase 5
Genes
HSA: 222537(HS3ST5) 9957(HS3ST1)
PTR: 471144(HS3ST1) 744298(HS3ST5)
PPS: 100967734(HS3ST1) 100979280(HS3ST5)
GGO: 101134668(HS3ST1) 101154208(HS3ST5)
PON: 100445561(HS3ST5) 100449950(HS3ST1)
NLE: 100592585(HS3ST5) 100601459(HS3ST1)
MCC: 693684(HS3ST5) 713374(HS3ST1)
MCF: 101865986(HS3ST1) 102141292(HS3ST5)
CSAB: 103240530(HS3ST5) 103246360(HS3ST1)
RRO: 104657111(HS3ST5) 104657730(HS3ST1)
RBB: 108512071(HS3ST5) 108529481(HS3ST1)
CJC: 100392281(HS3ST1) 100402689(HS3ST5)
SBQ: 101038172(HS3ST5) 101043463(HS3ST1)
MMU: 15476(Hs3st1) 319415(Hs3st5)
RNO: 294449(Hs3st5) 84406(Hs3st1)
CGE: 100759378(Hs3st1) 100763965(Hs3st5)
NGI: 103728889(Hs3st1) 103733777(Hs3st5)
HGL: 101698728(Hs3st1) 101710328(Hs3st5)
CCAN: 109677593(Hs3st5) 109694636(Hs3st1)
OCU: 100343879(HS3ST1) 100346980(HS3ST5)
TUP: 102468225(HS3ST5) 102481838(HS3ST1)
CFA: 403672(HS3ST1) 481968(HS3ST5)
AML: 100478675(HS3ST5) 105241055(HS3ST1)
UMR: 103658356(HS3ST1) 103676972(HS3ST5)
ORO: 101364972(HS3ST5) 101380812(HS3ST1)
FCA: 101088412(HS3ST5) 101090103(HS3ST1)
PTG: 102952040(HS3ST5) 102965928(HS3ST1)
AJU: 106968920(HS3ST5) 106975621(HS3ST1)
BTA: 538691(HS3ST1) 540355(HS3ST5)
BOM: 102279879(HS3ST5) 102279923(HS3ST1)
BIU: 109560679(HS3ST1) 109563804(HS3ST5)
PHD: 102325819(HS3ST5) 102327107(HS3ST1)
CHX: 102169088(HS3ST1) 102176690(HS3ST5)
OAS: 101104909(HS3ST1) 101107448(HS3ST5)
SSC: 100155403(HS3ST5) 100628223(HS3ST1)
CFR: 102503844(HS3ST5) 102515681(HS3ST1)
CDK: 105085126(HS3ST1) 105099225(HS3ST5)
BACU: 103015413(HS3ST1) 103016478(HS3ST5)
LVE: 103068414(HS3ST5) 103089700(HS3ST1)
OOR: 101277155(HS3ST5) 101277987(HS3ST1)
ECB: 100056091(HS3ST1) 100066938(HS3ST5)
EPZ: 103561316(HS3ST5)
EAI: 106847041(HS3ST5) 106847606(HS3ST1)
MYB: 102239991(HS3ST5) 102241744(HS3ST1)
MYD: 102756820(HS3ST1) 102773229(HS3ST5)
HAI: 109375997(HS3ST1) 109384729(HS3ST5)
RSS: 109452137(HS3ST1) 109453290(HS3ST5)
PALE: 102879059(HS3ST5) 102886681(HS3ST1)
LAV: 100673247(HS3ST5) 100673479(HS3ST1)
MDO: 100012960(HS3ST1) 100022809(HS3ST5)
SHR: 100917710(HS3ST1) 100926311(HS3ST5)
OAA: 100079950(HS3ST5) 100087772(HS3ST1)
GGA: 422840(HS3ST1) 423822(HS3ST1L) 428621(HS3ST5)
MGP: 100541206 100541289(HS3ST5) 100546598(HS3ST1)
CJO: 107311893(HS3ST5) 107314022(HS3ST1) 107315944
APLA: 101791819(HS3ST5) 101793237(HS3ST1) 101804190
ACYG: 106032639(HS3ST5) 106035306(HS3ST1) 106038121
TGU: 100217958 100218001 100221445(HS3ST5) 100221819(HS3ST1)
GFR: 102031527(HS3ST1) 102038656(HS3ST5) 102043838
FAB: 101809457(HS3ST5) 101814866(HS3ST1) 101816899
PHI: 102102605(HS3ST1) 102104930(HS3ST5) 102109527
PMAJ: 107202537(HS3ST5) 107203500(HS3ST1) 107206970
CCAE: 111927167(HS3ST5) 111928184(HS3ST1) 111931213
CCW: 104690727(HS3ST1) 104691254(HS3ST5) 104696091
FPG: 101919371 101923794(HS3ST5) 101923963(HS3ST1)
FCH: 102053103(HS3ST1) 102054204 102057031(HS3ST5)
CLV: 102091303(HS3ST1) 102094670 102096271(HS3ST5)
EGZ: 104129170 104131984(HS3ST5) 104134473(HS3ST1)
AAM: 106489636(HS3ST5) 106494700 106498671(HS3ST1)
ASN: 102376753(HS3ST1) 102378310(HS3ST5) 102385617
AMJ: 102560943(HS3ST5) 102573417(HS3ST1L1) 102574400(HS3ST1)
PSS: 102444953 102452315(HS3ST5) 102452655(HS3ST1) 102460581
CMY: 102930016 102930942(HS3ST1) 102936568(HS3ST5) 102947282
CPIC: 101935011 101940499(HS3ST5) 101942391(HS3ST1) 101947422
ACS: 100560134 100562373(hs3st1) 100563170 100566144(hs3st5)
PVT: 110072143 110079727(HS3ST1) 110088773(HS3ST5)
PBI: 103049756(HS3ST1) 103051973 103053046 103058998(HS3ST5)
GJA: 107107831 107118809(HS3ST1) 107123623 107124746(HS3ST5)
XLA: 108696134 108697406 108716820(hs3st5.L) 398971(hs3st1.L) 399064(hs3st1.S)
XTR: 100125092 100145790(hs3st5) 496940(hs3st1)
NPR: 108785216(HS3ST1) 108788983 108789380(HS3ST5)
DRE: 557111(hs3st1l1) 565201(hs3st1)
IPU: 108255327 108255574(hs3st5) 108260334(hs3st1) 108262815(HS3ST1)
AMEX: 103023335(hs3st1) 103029341(hs3st5) 103036825 103039507
TRU: 101061220 101065122 101076430(hs3st1) 101079258(hs3st5)
LCO: 104921922 104923456 104928689(hs3st1) 104932424(hs3st5)
MZE: 101469297 101475274 101475421(hs3st1) 101477610(hs3st5)
OLA: 101165781(hs3st5) 101166804(hs3st1) 101169760 101174369
XMA: 102216507(hs3st5) 102220745 102232490 102232809(hs3st1)
KMR: 108228841(hs3st5) 108231121(hs3st1) 108242749 108246557
CSEM: 103380640(hs3st5) 103387477 103390605(hs3st1) 103397870
SDU: 111220657 111227346 111232616(hs3st1) 111240260(hs3st5)
HCQ: 109515940(hs3st1) 109523019
BPEC: 110155521(hs3st5) 110158917(hs3st1) 110161364 110172130
MALB: 109951332 109960951 109966559(hs3st5) 109974538(hs3st1)
ELS: 105007233 105017884(hs3st5) 105025937 105029317(hs3st1)
SFM: 108924148(hs3st1) 108925805 108929368(hs3st5)
LCM: 102345335(HS3ST5) 102346461(HS3ST1) 102352685 102364972
CMK: 103175792(hs3st1) 103180481 103182576(hs3st5) 103187765
SPU: 594559
DME: Dmel_CG33147(Hs3st-A)
DSI: Dsimw501_GD11407(Dsim_GD11407)
MDE: 101898689
AAG: 5570723
AME: 412956
BIM: 105680199
BTER: 100645554
SOC: 105207583
AEC: 105149682
ACEP: 105619000
PBAR: 105432185
HST: 105192616
DQU: 106750173
CFO: 105258568
LHU: 105677087
PGC: 109857918
PCF: 106791895
NVI: 100123415
MDL: 103578076
TCA: 660198
DPA: 109533375
BMOR: 101740692
PMAC: 106720254
PRAP: 110991581
HAW: 110369990
API: 100167476
CLEC: 106674312
FCD: 110844017
TUT: 107367708
BMY: Bm1_42350
TSP: Tsp_02737
ADF: 107354956
AQU: 100641408
 » show all
Taxonomy
Reference
1  [PMID:3139669]
  Authors
Kusche M, Backstrom G, Riesenfeld J, Petitou M, Choay J, Lindahl U.
  Title
Biosynthesis of heparin. O-sulfation of the antithrombin-binding region.
  Journal
J Biol Chem 263:15474-84 (1988)
Reference
2  [PMID:8900197]
  Authors
Shworak NW, Fritze LM, Liu J, Butler LD, Rosenberg RD.
  Title
Cell-free synthesis of anticoagulant heparan sulfate reveals a limiting converting activity that modifies an excess precursor pool.
  Journal
J Biol Chem 271:27063-71 (1996)
DOI:10.1074/jbc.271.43.27063
Reference
3  [PMID:8900198]
  Authors
Liu J, Shworak NW, Fritze LM, Edelberg JM, Rosenberg RD.
  Title
Purification of heparan sulfate D-glucosaminyl 3-O-sulfotransferase.
  Journal
J Biol Chem 271:27072-82 (1996)
DOI:10.1074/jbc.271.43.27072
  Sequence
[hsa:9957]
Reference
4  [PMID:9346953]
  Authors
Shworak NW, Liu J, Fritze LM, Schwartz JJ, Zhang L, Logeart D, Rosenberg RD.
  Title
Molecular cloning and expression of mouse and human cDNAs encoding heparan sulfate D-glucosaminyl 3-O-sulfotransferase.
  Journal
J Biol Chem 272:28008-19 (1997)
DOI:10.1074/jbc.272.44.28008
  Sequence
[hsa:9957] [mmu:15476]
Reference
5  [PMID:10026187]
  Authors
Zhang L, Yoshida K, Liu J, Rosenberg RD.
  Title
Anticoagulant heparan sulfate precursor structures in F9 embryonal carcinoma cells.
  Journal
J Biol Chem 274:5681-91 (1999)
DOI:10.1074/jbc.274.9.5681
Reference
6  [PMID:11375390]
  Authors
Zhang L, Lawrence R, Schwartz JJ, Bai X, Wei G, Esko JD, Rosenberg RD.
  Title
The effect of precursor structures on the action of glucosaminyl 3-O-sulfotransferase-1 and the biosynthesis of anticoagulant heparan sulfate.
  Journal
J Biol Chem 276:28806-13 (2001)
DOI:10.1074/jbc.M100204200
Other DBs
ExplorEnz - The Enzyme Database: 2.8.2.23
IUBMB Enzyme Nomenclature: 2.8.2.23
ExPASy - ENZYME nomenclature database: 2.8.2.23
BRENDA, the Enzyme Database: 2.8.2.23
CAS: 183257-54-7

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